JOURNAL OF FLUORESCENCE
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2025
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IF 3.1
Li Chen,Niu Shengjie,Yu Siwen,Li Hang,Zhang Shengrui,Cao Xiaoyan,Li Lihua,Dai Huiping,Sun Jiangjiang,Li Hua,Wang Qin
The freshness of food is directly connected with safety, hygiene, and human health. Accurate quantification of biogenic amine level is crucial for assessing the food quality. Therefore, a dual-excitation ratiometric fluorescent probe MCCN based on hemicyanine dye for the examination of biogenic amines was constructed. After the addition of biogenic amines to the MCCN solution, an elimination reaction occurred after a nucleophilic addition reaction to form Schiff base MC-CA , resulting in colorimetric and ratiometric fluorescence responses. The probe MCCN solution was blue. After successively adding biogenic amines (cadaverine, n-butylamine and spermidine), the fluorescence emission intensity of probe MCCN at 709 nm reduced under excitation at 650 nm, meanwhile a new fluorescence emission peak emerged at 545 nm gradually increased under excitation at 450 nm, accompanying by a visible color variation from blue to yellow. The probe MCCN also displayed remarkable selectivity toward biogenic amines over other analytes. Based on the above excellent characteristics, the probe MCCN was successfully utilized to detect biogenic amines in the extract supernatant of shrimp and fish during the spoilage process. In addition, photographing the MCCN -loaded food extract supernatant enabled the correlation of color parameters with storage time, affording an accurate and simple method to reveal food spoilage process. The excellent sensing performance makes the MCCN probe a convenient and accurate screening platform for real-time assessing food freshness.
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PubMed
高影响因子
DYES AND PIGMENTS
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2025
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IF 4.2
Boyuan Li,Wenjie Zhao,Ningning Wang,Lingbo Qu,Lin Zhang,Zhaohui Li
Hydrogen peroxide (H 2 O 2 ), a common additive in food industry, poses significant health risks when overused. Traditional detection methods face challenges such as reliance on bulky instrumentation, and poor portability. To address these limitations, we developed a novel hemicyanine-based probe (BIY) for rapid, portable, and visual H 2 O 2 detection in food. The probe possesses a rationally designed acceptor-π-acceptor architecture, which transforms into a donor-π-acceptor structure upon H 2 O 2 -triggered boronate ester cleavage. This transformation elicits a fluorescence activation phenomenon at 490 nm based on the enhanced intramolecular charge transfer mechanism. This method exhibited excellent sensitivity and selectivity. A smartphone-assisted sensing platform was further developed to quantify H 2 O 2 via RGB analysis, achieving on-site results comparable to those of laboratory instruments. The probe has validated its practical utility in milk samples with good recoveries, showing great potential in food industry.
研究领域:
分析化学
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食品科学
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生物传感
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荧光光谱分析
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便携式检测技术
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Lu Fan,Yulong Jin,Aiguo Zhang,Youzhen Feng,Kai Wang
In this study, A fluorescent/photoacoustic dual-modal probe HD-MAO for hemicyanine derivatives was successfully designed and synthesized, achieving high-sensitivity and high-specificity detection of monoamine oxidase A (MAO-A). Through the ingenious combination of the specific MAO-A recognition group and the hemipanocyanine fluorophore, the probe produced a significant enhancement of the intramolecular charge transfer (ICT) effect after enzymatic digestion, showing the synergistic activation characteristics of fluorescence (∼730 nm) and photoacoustic (∼700 nm) signals, with a detection limit of 0.806 mg/L. In the diet-induced obese mouse model, HD-MAO successfully revealed a significant increase in liver MAO-A activity, which was highly consistent with the serum test results, providing molecular imaging evidence for the early diagnosis of metabolic diseases. The probe behaves stably under physiological conditions and shows no cross-reaction to common interferents. Serum detection experiments further confirm its clinical applicability. This study not only provides an innovative tool for the research on MAO-A-related mechanisms, but also opens up a new approach for the early diagnosis and treatment monitoring of metabolic diseases.
研究领域:
生物成像
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分子诊断
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酶检测
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代谢疾病
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生物医学光学
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探针开发
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PubMed
高影响因子
DYES AND PIGMENTS
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2025
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IF 4.2
Xin-Ke Shao,Lu Fan,Jie-Li Zhou,Kai Wang
The development of novel molecular probes for real-time monitoring of aminopeptidase N (APN) activity is crucial for understanding inflammatory bowel disease (IBD) progression. Here, we present a new probe, HDAPN, a near-infrared (NIR) activatable fluorescent probe based on a hemicyanine scaffold, designed for selective APN detection. Structural characterization confirmed the molecular integrity of the probe, while spectroscopic studies demonstrated its exceptional photostability and response. HDAPN exhibits a remarkable "turn-on" response (720 nm emission) upon APN-mediated cleavage, with outstanding sensitivity (LOD = 0.82 ng/mL) and linearity under physiological conditions. The probe showed minimal cross-reactivity with interferents (e.g., ROS, metal ions, and other enzymes) and successfully detected LPS-induced APN upregulation in RAW264.7 macrophages. In DSS-induced colitis models, HDAPN enabled non-invasive visualization of APN activity, with fluorescence intensity correlating with disease severity (2-fold increase in severe colitis). Comprehensive toxicity evaluations confirmed its biosafety in vitro and in vivo . These findings highlight the potential of HDAPN as a robust tool for studying APN-associated pathophysiology and therapeutic development.
研究领域:
生物化学
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分子成像
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炎症性肠病
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荧光探针
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酶学
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生物医学光学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Jing-Jing Qiao,Lu Fan,Jie-Li Zhou,Kai Wang
Cysteine (Cys) plays pivotal roles in redox homeostasis and metabolic regulation, with its dysregulation implicated in obesity-related hepatic disorders. Here, we developed a sulfur-substituted hemicyanine-based nanoprobe (Cys-NP) for photoacoustic imaging of hepatic Cys dynamics. The Cys-responsive molecular probe (Cys-MP) was encapsulated in mPEG-DSPE nanoparticles to overcome aqueous solubility limitations, yielding monodisperse nanoparticles with an average diameter of 28.7 nm and excellent colloidal stability. Sulfur substitution endowed the probe enhanced photoacoustic signals compared to oxygenated analogs, while selective Cys recognition triggered a bathochromic shift and yielded a detection limit of 0.13 μM. In high-fat-diet-induced obese mice, Cys-NP revealed significantly elevated hepatic Cys levels correlating with histopathological steatosis and hypercholesterolemia. This work establishes a nanotechnology-enabled strategy for mapping redox metabolism in metabolic diseases, offering both mechanistic insights and diagnostic potential.
研究领域:
纳米技术
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分子成像
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代谢性疾病
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氧化还原生物学
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生物医学工程
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PubMed
高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Wuyan Hou,Lichao Li,Chang Liu,Boying Ding,Jiahao Cui,Longni Chen,Yuan Qiu,Zhiqiang Jiao,Juan Zhang,Shougen Yin,Xiaoming Wu
As an essential component in blue perovskite light-emitting diodes (PeLEDs), functionally tailored hole transport materials (HTMs) play a decisive role in reducing the hole injection barrier, mitigating interfacial defects, and thus enhancing device performance. Herein, we report two solution-processable D-π-A hemicyanine dyes, which exhibit asymmetric/symmetric configurations and contain electron-withdrawing groups and Lewis base donors. As multifunctional HTMs, two dyes not only present high hole mobilities with improved energy level alignment in PeLEDs, but also enable the perovskite defects passivation and n phases manipulation. Interestingly, the symmetric dye achieves dual-channel hole injection and multisite anchoring for the quasi-2D perovskite (PEACsPbBr2.1Cl0.9). The passivated perovskite film forms an augmented distribution of large n phases and suppressed small n phases, thereby promoting radiative exciton recombination. Consequently, symmetric dye-treated PeLED exhibits an impressive blue electroluminescence (EL) with a maximum luminance reaching 5842 cd m–2, a peak external quantum efficiency (EQE) of 13.71% and an improved operational half lifetime (T50) of 6.7 min at 100 cd m–2, demonstrating its performance advantages over the reported PEACsPb(BrxCl1–x)3 PeLEDs. This work provides an innovative perspective for designing multifunctional HTMs by conformation engineering and gains insight into the interfacial contact for high performance perovskite optoelectronics.
研究领域:
材料科学
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光电子学
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纳米技术
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应用物理学
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化学工程
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高影响因子
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