Journal of Functional Foods
·
2024
·
IF 3.8
Xiling Wang,Yanan Cheng,Bing Zheng,Yi Chen,Jianhua Xie,Xiaobo Hu,Xiaoting Qin,Jiajun Song,Yue Qiu,Qiang Yu
The traditional ‘nine-steam-nine-bask’ method of Polygonatum cyrtonema Hua (PCH) has been controversial due to nine-cycle requirement. This study employed in vitro digestion to investigate changes in bioactive substances, bioaccessibility, and antioxidant activity of PCH at different steam-bask times. It revealed the correlation between bioactive substances and antioxidant activity in PCH, and validated the ‘nine-steam-nine-bask’ process’s efficacy. In undigested samples, total phenol content (TPC), reducing sugar content (RSC), and DPPH and ABTS activities increased with more cycles, peaking in the nine-cycle sample at 7.627 mg GAE/g DW, 465.333 mg GE/g DW, 17.698, and 36.986 μmol VCE/g DW. Similar trends were observed throughout digestion. Total sugar (755.96 mg GE/g DW) and total saponin content (1.35 mg DE/g DW) plateaued during gastrointestinal digestion. Correlation analysis further revealed close relationships between bioactive substances and antioxidant activity. Multivariate statistical analysis confirmed that nine cycles maximized the distinction between steam-bask and non-steam-bask PCH.
研究领域:
药理学
·
食品科学
·
中医药
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 24
·12883-12895
·
IF 6.317
Wei Liu,Taili Shao,Lei Tian,Zhengrui Ren,Lan Gao,Zhiyan Tang,Zheng Fang,Pingchuan Yuan,Chunyan Liu,Jikun Li,Guodong Wang,Jun Han
The chemical structure and pharmacological activity of Polygonatum cyrtonema Hua polysaccharides have garnered significant attention in recent years. In this study, a homogeneous polysaccharide, PCP1, was extracted from P. cyrtonema Hua rhizomes and purified. Monosaccharide composition analysis showed that PCP1 is primarily composed of fructose, glucose, and mannose. Chemical structure analysis showed that the main chain of PCP1 is composed mainly of →1)-β-D-Fruf-(2→ and →1,6)-β-D-Fruf-(2→, with small amounts of →6)-α-D-Glcp-(1→, →4)-β-D-Manp-(1→, and β-D-Glcp-(1→. The side chain is β-D-Fruf-(2→ linked at C-6 of →1,6)-β-D-Fruf-(2→. In vivo experiments showed that PCP1 mitigates liver pathological damage, improves abnormal lipid metabolism and oxidative stress, promotes the production of short-chain fatty acids, and balances the composition of the intestinal microbiota in non-alcoholic fatty liver disease (NAFLD) mice. Thus, PCP1 can be used as a natural ingredient in functional foods for the treatment of NAFLD.
研究领域:
药理学
·
生物化学
·
营养学
·
胃肠病学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 7
·2200823
·
IF 3.1
Gaodan Liu,Simin Feng,Minghui Sui,Bilian Chen,Peilong Sun
In this study, quartz sand with particularly sharp nanoscale edges acted like a nanoscale knife physically cut cells of Polygonatum cyrtonema Hua into nanosized particles and was synergized with natural deep eutectic solvent to extract steroidal saponins of Polygonatum cyrtonema Hua. The natural deep eutectic solvent (choline chloride-lactic acid)-synergistic quartz sand-assisted extraction was optimized using response surface methodology. The steroidal saponins purified with AB-8 macroporous resin were identified using ultra-high-performance liquid chromatography-triple time of flight mass spectrometry. The results showed that the experimental total saponins content value (36.97 ± 0.12 mg dioscin equivalent/g dry weight) at optimal extraction conditions with a temperature of 68°C, a rotational speed of 20 400 rpm, shear time of 4.3 min, the liquid-solid ratio of 38 ml/g, was close to the maximum possible theoretical value (36.64 mg dioscin equivalent/g dry weight). A total of 20 steroidal saponins were identified, among which the content of (25 R )-Kingianoside E was the highest (102.66 ± 3.47 mg/g). Furthermore, a new steroid saponin (3 β ,25 S )-26-( β -D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl 4- O - β -D-glucopyranosyl- β -D-galactopyranoside+Glc was found for the first time. These results revealed that natural deep eutectic solvent-synergistic quartz sand-assisted extraction was an efficient and green method to extract a variety of steroidal saponins.
研究领域:
药学化学
·
分析化学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yanyan Zhang,Shengjun Ma,Haixia Li,Peizi Liu,Keyi Chen,Wei Cai
Research on Polygonatum cyrtonema Hua has focused extensively on its main active components, yet antioxidant peptides remained less explored. This study optimized the preparation process of antioxidant peptides from P. cyrtonema via protein hydrolysis using response surface methodology. The peptide mixture was separated into four fractions using ultrafiltration and G-25 gel chromatography, based on antioxidant capacity. Notably, the F2 exhibited significantly higher Fe 3 ⁺ reducing power (17.17%) and ABTS· radical scavenging rate (30.1%) compared to other fractions at the same concentration. Characterization by Nano-LC-Q Exactive Plus quadrupole Orbitrap MS revealed that F2 contained 11,704 peptides, markedly more than the endogenous (2,454) and crude protein peptides (2,334). Bioinformatics and molecular docking identified three peptides—QFLPF, FVPF, and FPNLF—as having the highest antioxidant potential. These peptides increased cell viability in oxidative damage-induced HepG2 cells by 6.39%, 14.51%, and 9.98%, respectively, compared to the model group. This study lays the groundwork for future mechanistic investigations into the antioxidant peptides of P. cyrtonema.
研究领域:
药理学
·
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）