ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yanxia Ma,Xianglin Liao,Yingtong Zhao,Liyu Qiu,Yao Yao,Shizhong Wang,Xixiang Yang,Xiaogang Hu
Tetracycline is extensively used as an antibiotic in animal husbandry, and there arose an increase in antibiotic resistance genes in the environment, posing a threat to human health. Motivated by this, a magnetic molecularly imprinted material based on synergistic recognition (1 + 1>2) was constructed and coupled with high-performance liquid chromatography to detect ultra-trace tetracycline in complicated samples. In this case, the molecularly imprinted polymers were synthesized via a “one-pot” method and acted as recognition elements on the surface of silica-coated ferroferric oxide particles. Aptamers and β-cyclodextrin, as functional monomers , had a synergistic effect on the recognition of tetracycline and the synergistic recognition factor was 1.7. Meanwhile, the material exhibited high selectivity to tetracycline with an imprinting factor of 7.6. In addition, compared to being modified on the surface, the stability of the aptamers was effectively improved by cross-linking in the molecularly imprinted polymer framework. Relevant experimental conditions, such as buffers, concentration of magnesium ions and adsorption time, were optimized. As a result, the method showed a limit of detection of 1.0 μg L −1 and the linearity range of 0.005–0.5 mg L −1 , as well as certain reproducibility and stability. Furthermore, when applied for the analysis of animal feed samples, a significant reduction of matrix interference was observed with satisfactory recoveries (85.0–111.5%), which emphasized the good accuracy and practicability of the established method. For these advantages, the proposed method represents a versatile and powerful tool for the separation and detection of small molecule compounds in complicated real samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Wenbin Zhang,Yuzeng Li,Nurimangul Muntiza,Wenquan Ji,Zhen Fan,Qinran Li,Jin Zhao,Hongfeng Zhang,Qiliang Deng,Donglan Sun,Tianjun Liu
A dual-epitopes imprinted strategy for cytochrome c selective recognition assisted with γ-cyclodextrin host-guest interaction via N-terminal and C-terminal epitope's simultaneous imprinting and reversible addition-fragmentation chain transfer (RAFT) polymerization was developed. N-terminal and C-terminal nonapeptides of Cyt c (GI-9 and AE-9) were used simultaneously as the epitope to achieve collaborative recognition for cytochrome c. As a supramolecule, γ-cyclodextrin can encapsulate the aromatic functional groups of amino acid residues to capture the peptide and improve the corresponding spatial orientation for epitope or cytochrome c recognition by host-guest interaction. After the γ-cyclodextrin modification and dual-epitopes immobilization, the imprinted polymer was synthesized by RAFT polymerization with 4-cyano-4-(phenyl-carbonothioylthio) pentanoic acid as a chain transfer agent. After the template removal, the obtained dual-epitopes imprinted particles showed well binding ability to AE-9 (26.50 mg·g −1 , IF= 4.13), GI-9 (7.36 mg·g −1 , IF= 2.18) and cytochrome c (79.56 mg·g −1 , IF= 3.27). With the successive addition of RAFT agent, the imprinting factor rising of epitope peptide and cytochrome c further illustrated the regulation of imprinted polymer chains. The imprinted particles had the advantage for cytochrome c recognition compared to other proteins and good reusability with 82.60 % repeated reproduction rate after six cycles of adsorption and desorption. Furthermore, the selective recognition for cytochrome c in bovine serum proved its potentiality to be applied in complex biological samples. It indicated that the combination of dual-templates epitope imprinting, γ-CD host-guest interaction and RAFT polymerization provided an efficient method for collaborative protein recognition with well selectivity, reusability and stability.
研究领域:
分子印迹技术
·
蛋白质科学
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Kaicheng Zhang,Ming Yan,Yue Li,Faguang Ma,Yilin Wu
Membrane-based imprinted sites for achieving specific molecule transport and precise recognition have great potential to revolutionize nanofiltration technology . Nonetheless, how to efficiently prepare imprinted membrane structures with accurate identification - ultrafast molecular transport - high stability in mobile phase remains a key issue and serious challenge. Herein, we have developed a dual-activation strategy to constructing nanofluid-functionalized membranes with double imprinted nanoscale channels (NMDINCs), realizing ultrafast transport performance as well as structure&size-exclusion selectivity in allusion to particular compounds. The resultant NMDINCs, founded on principal nanofluid-functionalized construction companied by the boronate affinity sol-gel imprinting systems, illustrated that delicate regulation towards polymerization framework as well as functionalization belonging to distinctive membrane structures was crucial for realizing ultrafast molecules transport combined with prominent molecules selectivity. The synergistic recognition of covalent bonds and non-covalent bonds driven by two functional monomers effectively realized the selective recognition to template molecules, leading to the high selective separation factors of Shikimic acid (SA)/ Para hydroxybenzoic acid（PHA）, SA/ P nitrophenol（PN）and catechol（CL）for 8.9, 8.14 and 7.23, respectively. The dynamic consecutive transport outcomes exhibited that numerous SA-dependent recognition sites could still keep reactivity under pump-driven permeation pressure for appreciable time, forcefully proving the successful construction as to high-efficiency membrane-based selective separation system. It is anticipated that this strategy as to the in situ introduction of nanofluid-functionalized construction into porous membrane would hold great promise in preparing high-intensities membrane-founded discriminating separation systems, which was equipped with prominent consecutive permeability as well as excellent selectivity.
研究领域:
材料科学
·
化学工程
·
纳米技术
·
分离技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Hai Chi,Yue Xiao,Xuefei Wen,Houqi Ning,Xingzhong Zhang,Hongrui Chen,Jie Tang
This study developed an electrochemical sensor incorporating a dual recognition strategy that integrates molecularly imprinted polymer (MIP) and aptamers for the ultra-sensitive detection of zearalenone (ZEN) in grains. Exploiting the high specific surface area of the Cu-Zr bimetallic metal-organic framework-818 (MOF-818), aptamers were covalently immobilized on the olefin-functionalized MOF-818 surface via thioenol reaction. Employing 3-thiopheneacetic acid functionalized gold nanoparticles (3-TAA@AuNPs) as functional monomers, nanoscale molecularly imprinted cavities were electro-polymerized onto the modified electrode surface in the presence of the ZEN template. This synergistic recognition system integrates the aptamer's specific binding capability with the spatial complementarity of MIP, displaying an excellent linear response over a concentration range of 10 −13 to 10 −5 g/L and a detection limit as low as 2.8 × 10 −14 g/L. Analysis of real samples exhibited ZEN recoveries ranging from 96.5 % to 105.0 % (RSD <5.2 %) in corn and wheat matrices, confirming its practical applicability for rapid screening of fungal toxins in agricultural commodities.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
纳米技术
·
电化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Acta Pharmaceutica Sinica B
·
2024
·
IF 14.7
Yutian Lei,Yuan Shen,Feng Chen,Rui He,Zhang Zhang,Ying Zhou,Jin-Chen Yu,Jacques Crommen,Zhengjin Jiang,Qiqin Wang
The dynamic tracking of antibody‒drug conjugates (ADCs) in serum is crucial. However, a versatile bioanalytical platform is lacking due to serious matrix interferences, the heterogeneity and complex biotransformation of ADCs, and the recognition deficiencies of traditional affinity technologies. To overcome this, a multiepitope recognition technology (MERT) was developed by simultaneously immobilizing CDR and non-CDR ligands onto MOF@AuNPs. MERT’s excellent specificity, ultrahigh ligand density, and potential synergistic recognition ability enable it to target the different key regions of ADCs to overcome the deficiencies of traditional technologies. The binding capacity of MERT for antibodies is ten to hundred times higher than that of the mono-epitope or Fc-specific affinity technologies. Since MERT can efficiently capture target ADCs from serum, a novel bioanalytical platform based on MERT and RPLC‒QTOF-MS has been developed to monitor the dynamic changes of ADCs in serum, including the fast changes of drug-to-antibody ratio from 3.67 to 0.22, the loss of payloads (maytansinol), and the unexpected hydrolysis of the succinimide ring of the linker, which will contribute to clarify the fate of ADCs and provide a theoretical basis for future design. In summary, the MERT-based versatile platform will open a new avenue for in-depth studies of ADCs in biological fluids.
研究领域:
药学
·
生物分析化学
·
生物技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）