BIOMEDICAL CHROMATOGRAPHY
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2023
·Vol. 37
·期 12
·e5743
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IF 1.8
Wei-Han Yang,Jing-Wen Hao,Nai-Dong Chen,Jiao Li
The determination of monosaccharides is crucial for studying the structure of polysaccharides and the composition of free monosaccharides in living organisms. Based on previous derivatization gas chromatography–mass spectrometry (GC–MS) methods, we aimed to develop a novel analytical protocol for better quantifying monosaccharides. In this study, sugar alcohol acetylation, saccharonitrile acetylation, silylation and a combination of sugar alcohols acetylation and saccharonitrile acetylation were compared. The optimal method was verified with the monosaccharide determination of four polysaccharides and four free monosaccharides from Dendrobium . The results showed that the novel combined derivatization method was superior to the other three methods in terms of content analysis of monosaccharides. Furthermore, it possessed good linearity (all calibration curves showed relative coefficients ≥ 0.999), sensitivity, precision (relative standard deviation < 2%), and accuracy (recovery, 95.7–105%). Finally, the novel method established in this study was successfully employed in determining the monosaccharide composition of four polysaccharides and four free monosaccharide samples from Dendrobium .
研究领域:
分析化学
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植物化学
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药学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2022
·
IF 5.089
Huixing Xu,Xin Ye,Xiaoyu Shi,Heng Zhong,Daoping He,Binbin Jin,Fangming Jin
Catalytic production of lactic acid (LA) from biomass has attracted increasing attention due to the wide applications of LA. However, the industrial-scale production of LA is seriously limited by the complex synthetic process of efficient catalyst. In this work, we applied commercially available ZnO as a simple and facile heterogeneous catalyst to catalyze the conversion of glucose into LA under hydrothermal conditions. The ZnO possesses abundant strong Lewis acid and base sites and acts as bifunctional catalyst to promote the cascade reactions from glucose to LA, leading to a high yield of 41.5%. The robustness of catalytic process and the wide scope of applicable substrates like monosaccharides and disaccharides further extend the application of hydrothermal biomass conversion using facile catalysts.
研究领域:
化学工程
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可再生能源
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催化化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Jianping Li,Xionghui Ma,Mengxi Li,Yun Zhang
A novel glycoprotein imprinting strategy was proposed and was applied to the detection of the carbohydrate antigen 19-9. The glycosylated complex of glycoprotein was used as template to construct a glycosyl imprinted sensor. The eluted imprinted cavities showed good affinity for template glycosyls and glycoproteins carrying template glycosyl. The effect of template saccharide structure on glycosyl imprinted sensors is further discussed. More complex template structures can lead to better sensor performance including selectivity and sensitivity. As a result, the polysaccharide imprinted sensor showed preeminent linear response to CA19-9 in the range of 0.1–5 U/mL, with a detection limit of 0.028 U/mL (3δ/K), while the linear of the monosaccharide imprinted sensor was 1–60 U/mL and the detection limit was 0.17 U/mL. The complex structure on the template surface provides more possibilities for the recognition of the template molecules, consequently, led to the significant anti-interference capability of the polysaccharide imprinted sensor. Furthermore, recoveries ranging from 93.0% to 103.5% were achieved when human serum samples were assayed using the polysaccharide imprinted sensor.
研究领域:
生物化学
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医学诊断
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分子生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 26
·39702-39711
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IF 5.19
Qiao Yongzhen,Yang Weisheng,Wang Xiu,Jiao Liang,Yang Yiqin,Wang Shumei,Bian Huiyang,Dai Hongqi
When old corrugated cardboard (OCC) is returned to the paper mill for repulping and reuse, the starch, which is added to the paper surface as a reinforcement agent, is dissolved into the pulping wastewater. Most of the OCC pulping wastewater is recycled to save precious water resources; however, during the water recycling process, the accumulation of dissolved starch stimulates microbial reproduction, which causes poor water quality and putrid odor. This problem seriously affects the stability of the papermaking process and product quality. In this study, phosphomolybdic acid (H 3 PMo 12 O 40 , abbreviated as PMo 12 ) was utilized to catalyze the waste starch present in papermaking wastewater to monosaccharides, realizing the resource utilization of waste starch. The results showed that the optimized yield of total reducing sugar (78.68 wt%) and glycolic acid (12.83 wt%) was achieved at 145 °C with 30 wt% PMo 12 at pH 2, which is equivalent to 91.51 wt% starch recovered from wastewater for resource utilization. In addition, the regeneration of the reduced PMo 12 was realized by applying a potential of 1 V for 2 h. Overall, this study has theoretical significance and potential application value for resource utilization of waste starch in OCC pulping process and cleaner management of OCC waste paper.
研究领域:
环境科学
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资源利用
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造纸工业
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DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 8
·1755
·
IF 4.35
Zhili Sheng,Junmei Liu,Bao Yang
Astragalus membranaceusis a functional food with multiple bioactivities. It presents differentiated health benefits due to origins. Polysaccharides (APS) are the leading bioactive macromolecules ofA. membranaceus, which are highly related to its health benefits. However, the effect of origin on the structural characteristics of APSs remains unclear. In this work, polysaccharides from four origins were isolated and identified by NMR. The results showed APSs of four origins had identical monosaccharide composition and glycosidic linkage. Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides. However, the level of methyl ester in pectins varied to a large extent. The molecular weight profiles of APSs were also different. Inner Mongolia APS had the largest percentage of 20–40 kDa polysaccharides. Molecular weight and methyl ester level were two important parameters determining the difference of APSs from four origins. These results were helpful to recognize the origin-related quality ofA. membranaceus.Keywords:glycosidic linkage;monosaccharide composition;NMR;pectin
研究领域:
植物学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2015
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IF 3.391
Chengzhu Ni,Binhe Zhu,Nani Wang,Muhua Wang,Suqing Chen,Jiajie Zhang,Yan Zhu
Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity ( r ⩾ 0.9994) and afforded low limits of detection ranging from 1.55 to 10.17 μg L −1 for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% ( n = 6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.
研究领域:
分析化学
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植物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Yuanhao Qiu,Zahra Batool,Rui Liu,Guoqing Sui,Bulei Sheng,Xinhua Zheng,Dan Xu
Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods. The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 10 3 Da, 1.18 × 10 4 Da and 4.23 × 10 4 Da, respectively. Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively. In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man. Immunological tests indicated that both PCPW and PCPS2 could increase NO production of RAW264.7 cells, and promote splenocyte proliferation. All three polysaccharides proved to be activators of NF-κB. Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.
研究领域:
植物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Separations
·
2023
·Vol. 11
·期 1
·2
·
IF 2.6
Meijuan Zhao,Fengyan Kuang,Yingyue Zhang,Guangping Lv
Monosaccharide composition analysis is essential to the structural characterization and research into the biological activity of polysaccharides. In this study, a systematic comparison was performed among commonly used monosaccharide composition analysis methods, including colorimetric and chromatographic methods. These were tested on 16 aldoses, ketoses, alditols, amino sugars, and uronic acids. Furthermore, the effect of hydrolysis methods was also investigated. The results showed that the phenol sulfuric acid method is greatly affected by the type of monosaccharide that is used as the reference substance. The determination of uronic acid using sulfuric acid carbazole is less affected by neutral sugars than that method using m-hydroxybiphenyl. The high-performance thin-layer chromatography (HPTLC) method can simultaneously analyze multiple samples and accurately determine the type of uronic acid. High-performance liquid chromatography (HPLC) can provide a good qualitative and quantitative analysis of aldose, amino sugars, and uronic acids, while gas chromatography–mass spectrometry (GC-MS) can detect aldose, ketose, and alditols. Fructose was detected in a large amount in inulin andCodonopsis pilosulaafter one-step hydrolysis, while it was totally destroyed in two-step hydrolysis. The release of galacturonic acid significantly increased after two-step hydrolysis in pectin andLycium barbarum, which indicated that one-step hydrolysis is not enough for acidic polysaccharides. The results of this study are beneficial for selecting appropriate hydrolysis and analysis methods in order to accurately analyze the monosaccharide compositions of natural polysaccharides.Keywords:polysaccharide;monosaccharide composition;hydrolysis condition;detection method
研究领域:
分析化学
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生物化学
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食品科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2023
·
IF 5.9
Shaochao Sun,Junqiang Zhang,Min Hong,Jialong Wen,Jiliang Ma,Runcang Sun
The utilization of photocatalysis for the simultaneous production of valuable fine chemicals and fuels from biomass-derived feedstocks holds significant promise; however, its practical implementation remains constrained. In this study, we present the development of an Fe-doped gallium nitride (GaN) catalyst treated by calcination (Fe@GaN-X) for the selectively conversion of biomass-based monosaccharides and xylan into lactic acid and carbon monoxide (CO). Fe@GaN-400 exhibited broader visible light absorption, lower resistance, and reduced photoluminescence intensity in comparison to pristine GaN, thereby affording exceptional photocatalytic activity (lactic acid yield: 71.38%; CO evolution rate: 385.74 μmol g −1 h −1 ). When monosaccharides and xylan were used as substrates, the Fe@GaN-400 system demonstrated outstanding photocatalytic activity, particularly in the case of xylan system (CO evolution rate = 923.21 μmol g −1 h −1 ). Additionally, the experimental results revealed the generation of distinctive reactive species, encompassing holes (h + ), superoxide anion (·O 2 - ), hydroxyl radical (·OH) and singlet oxygen ( 1 O 2 ) within this catalytic system. Remarkably, the ·O 2 - and h + exhibit heightened selectivity towards CO and lactic acid, respectively. This work establishes a novel pathway for the co-production of fine chemicals and fuels through the photocatalytic conversion of biomass.
研究领域:
化学工程
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环境科学
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材料科学
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产品关联
DOI
高影响因子
ENERGY & FUELS
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2016
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IF 2.835
Shurong Wang,Yurong Wang,Furong Leng,Junhao Chen
Sugars are among the main compounds in bio-oil produced by biomass pyrolysis. However, they can easily form coke, resulting in fast deactivation of the catalyst and severe blockage of the reactor during catalytic upgrading of bio-oil. Molecular distillation may be performed to retain sugars and pyrolytic lignin in the heavy fraction and thus to provide a primary stage for the subsequent sugar separation and utilization. A new method involving extraction, heat treatment, and column chromatography was introduced to separate further the aqueous phase obtained from the bio-oil heavy fraction by methanol–water extraction. Thus, monophenols and sugars were coextracted through these combined separation technologies. A fraction rich in monophenols was obtained by solvent extraction. Other impurities were further removed by heat treatment and column chromatography. An alcohol sedimentation method further separated the combined sugar-rich fraction into an ethanol-soluble fraction (SF-1) and an ethanol-insoluble fraction (SF-2). Gas chromatography–mass spectrometry, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and high-performance liquid chromatography were performed to characterize sugars in the obtained fractions. SF-1 mainly contained monosaccharides such as levoglucosan, glucose, and xylose, whereas SF-2 still had a small amount of cellobiose besides monosaccharides. The ultimate recovery rates for several identified monosaccharides were in the range of 75–86 wt %.
研究领域:
能源与环境科学
·
化学工程
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Baojie Zhu,Cunwu Chen,Jiaxian He,Shaoping Li,Jing Zhao
The interpretation of the chemical quality differences during Dendrobium huoshanense ( D. huoshanense ) traditional processing into ‘Fengdou’ is critical for scientifically evaluating the medicinal value, optimizing the processing methods, and ensuring the stability and predictability of its efficacy. In this study, the quality differences of polysaccharides during the processing of D. huoshanense were conducted utilizing high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), nuclear magnetic resonance (NMR) and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and high-performance anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD). The results showed that the concentration of non-starch D. huoshanense polysaccharides (NDHP) rose and exhibited greater stability during the processing. The molecular weight (Mw), molecular weight distribution, cumulative molar mass, radius of gyration (Rz), and monosaccharide composition molar ratio of NDHP did not have significant changes during the processing, and these parameters would be affected by the presence of starch. The NMR investigation indicated that the primary glycosidic linkages of β -1,4-Man p and β -1,4-Glc p , together with the acetyl group substitutions at O-2 and O-3 of 1,4-linked Man p , were unaltered. Additionally, the PACE and HPAEC-PAD profiles of acidic and enzymatic hydrolysates of NDHP were similar. These findings contribute to a deeper understanding of the changes in bioactive polysaccharides during the processing of D. huoshanense into ‘Fengdou’, providing theoretical support for improving its processing techniques.
研究领域:
中药学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Jing-Wen Hao,Nai-Dong Chen,Cun-Wu Chen,Fu-Cheng Zhu,De-Liang Qiao,Yong-Jun Zang,Jun Dai,Xiang-Wen Song,Han Chen
A rapid, green, low cost and nondestructive attenuated total reflection near infrared (ATR NIR) method was developed to quantify the total polysaccharide and the main monosaccharides mannose and glucose in Dendrobium huoshanense. Total 100 D. huoshanense samples from different places were analyzed using ATR NIR method. Potential outlying samples were initially removed from the collected NIR data using the PCA-Mahalanobis distance method. Spectral data preprocessing was studied in the construction of a partial least squares (PLS) model and six different signal pretreatment methods, including multiplicative scattering correction (MSC), standard normal transformation (SNV), first and second derivatives, the combination of MSC with the first derivative, and the combination of SNV with the first derivative, were compared. The results showed that the best signal pretreatment method was the spectral data pretreated by SNV combined with the first derivative due to it showed the lowest root-mean-square error of cross-validation (RMSECV), highest R 2 for both the polysaccharide and its main monosaccharides . In order to improve the performance of the model, the pretreated full spectrum was calculated by different wavelength selection method. The results showed that the optional wavelength selection model was the one simultaneously selecting the NIR wavelength ranges 7500–5750 cm −1 , 5250–4700 cm −1 , 4450–4300 cm −1 and 4200–4100 cm −1 because of the lowest RMSECV and the highest R 2 among the ten wavelength selection models. The external validation and the complete external validation confirmed the robustness and reliability of the developed NIR model. The contents of the total polysaccharide and the main monosaccharides are the essential quality assessment criterion for plant medicines while their traditional quantification methods involved sample destruction, tedious sample processing and non-environmentally friendly pretreatment, therefore, our study might provide an efficient technique tool for the rapid, green and nondestructive quantification of the total polysaccharide and the main monosaccharides for D. huoshanense and other rich-in-polysaccharide plant medicines.
研究领域:
植物学
·
分析化学
·
药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）