MOLECULES
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2019
·Vol. 24
·期 6
·1105
·
IF 3.06
Jiancheng Wang,Cuilin Cheng,Chao Xin,Zhenyu Wang
Fenugreek (Trigonella Foenum-Graecum) seeds flavonoids (FSF) have diverse biological activities, while the antidepressant-like effect of FSF has been seldom explored. The aim of this study was to evaluate the antidepressant-like effect of FSF and to identify the potential molecular mechanisms. LC-MS/MS was used for the determination of FSF. Chronic restraint stress (CRS) was used to establish the animal model of depression. Observation of exploratory behavior in the forced swimming test (FST), tail suspension test (TST) and sucrose preference test (SPT) indicated the stress level. The serum corticosterone (CORT) level was measured. The monoamine neurotransmitters (5-HT, NE and DA) and their metabolites, as well as monoamine oxidase A (MAO-A) enzyme activity in the prefrontal cortex, hippocampus and striatum, were evaluated. The protein expression levels of KLF11, SIRT1, MAO-A were also determined by western blot analysis. The results showed that FSF treatment significantly reversed the CRS-induced behavioral abnormalities, including reduced sucrose preference and increased immobility time. FSF administration markedly restored CRS induced changes in concentrations of serum corticosterone, prefrontal cortex neurotransmitters (NE, 5-HT and DA), hippocampus neurotransmitters (NE, 5-HT and DA) and striatum neurotransmitters (NE). FSF treatment exhibited significant inhibition of MAO-A activity in the prefrontal cortex and hippocampus. FSF also significantly down-regulated the KLF11, SIRT1 and MAO-A protein expression levels in the prefrontal cortex and hippocampus. These findings indicate that FSF could exhibit an antidepressant-like effect by down-regulating the KLF11/SIRT1-MAO-A pathways, inhibiting MAO-A expression and activity, as well as up-regulating monoamine neurotransmitters levels.Keywords:flavonoids;depression;monoamine neurotransmitters;monoamine oxidase A;KLF11;SIRT1
研究领域:
药理学
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神经科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Xuchun Qiu,Yibing Zhang,Jiarui Gao,Yiming Cui,Kejun Dong,Kun Chen,Yanhong Shi
Thimerosal (THI) has become a significant source of organic mercury pollutants in aquatic ecosystems, but there is limited information regarding its adverse effects on fish. In this study, zebrafish embryos were exposed to THI at 0 (control), 5.0, and 50 ng/L from 0–5 days post fertilization (dpf), and variations in their survival, development, behavior, free amino acid contents, and the biochemical responses involved in monoaminergic systems were examined. Although THI exposure did not significantly affect the survival, heart rate, or hatching time of zebrafish embryos, it substantially increased swimming velocity (136–154 % of the control) and reduced exploratory behavior (141–142 % of the control) in zebrafish larvae at 5 dpf. Exposure also significantly altered the amino acid contents (51–209 % of the control) and monoamine levels (70–154 % of the control) in zebrafish larvae, some of which displayed significant correlations with behavioral traits. THI significantly elevated dopamine receptor gene expression and monoamine oxidase activity in zebrafish larvae. Adding extra phenylalanine or tryptophan to the E3 medium facilitates the recovery of zebrafish larvae from the abnormal behaviors induced by THI. These findings reveal for the first time that THI exposure at the level of ng/L is sufficient to induce neurobehavioral toxic effects in the early life stages of zebrafish, and disrupting amino acid homeostasis is a critical underlying mechanism. This study provides valuable insights into the toxicity of THI to fish and highlights the importance of assessing its potential risks to aquatic ecosystems.
研究领域:
环境毒理学
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水生生物学
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神经科学
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发育生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Qiaoqiao Ci,Hong Yang,Yuanzhi Bian,Zheng Li,Jinhua Liu,JiFu Wei,Naidi Yang,Juhong Chen,Lin Li,Chengwu Zhang
Currently, the development of simple, fast, low-cost, and environmentally friendly MAO-A specific detection methods remains a huge challenge. Herein, a simple in situ fluorescent copolymer dots (FCDs)-based kit is constructed for the specific detection of monoamine oxidase-A in cell, tissue and human prostate cancer. The developed kit is depended on the high reaction activity between epinephrine and polyethyleneimine to form FCDs and the selective catalyzing activity of MAO-A on epinephrine. The gained FCDs are identified and characterized by high-resolution TEM, absorption and fluorescence spectrum. The present kit achieves highly sensitive detection of MAO-A with a limit of detection (LOD) of 20 ng/mL as well as screening of inhibitor with an IC50 (the concentration responding to 50% inhibition) of 1.071 μM by using clorgyline as a model. The MAO-A and MAO-B as the two isoforms of MAO are discriminated successfully, demonstrating the excellent selectivity of our kit. Noteworthy, the proposed method is applicable for measuring MAO-A not only from serum samples, but also for endogenous MAO-A in cells, mouse tissues and human prostate tumors with satisfied results. The proposed kit will be a great potential tool for human prostate cancer (PCa) diagnosis and the development of anti-PCa drug screening.
研究领域:
癌症生物学
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分析化学
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生物医学工程
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药理学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Shuai Lan,ChenSa Zhang,YuLin Li,Sibo Shen,Dongjun Wang,Kui Niu
In order to mimic the emissive mechanism of green fluorescent protein (GFP) by using chemical reagents, we systematically explored the micelle-triggered fluorescent system formed by non-conjugated simple amine molecules and cyclopentanol (CPA). In comparison with its amphiphilic ring-type homologues and linear homologues, we found that the fluorescent properties of the prepared systems were highly sensitive to surrounding environment. The present works help us get deeper cognition on the abnormal fluorescence mechanism, and provide a novel way to design highly efficient luminescent systems via green, facile and low-cost procedures, which was fundamentally different from the traditional method. Simultaneously, fluorescent micelles exhibit specific quenching of Fe3+, which can be combined with chestnut lignin to prepare Fe3+ detection and adsorption materials.
研究领域:
生物化学
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材料科学
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环境科学
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DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2020
·
IF 2.897
Jiao Yang,Qingyi Huang,Huan Liu,Xiong Zhou,Zhuoquan Huang,Quansheng Peng,Chunhong Liu
Environmental endocrine disruptors 4-nonylphenol (NP) and 4-tert-octylphenol (OP) may cast huge harm to human health. We used a rat model to observe the influence of NP or/and OP exposure on anxiety-related behaviors and the underlying mechanisms. Eighty male Sprague-Dawley (SD) rats were randomly divided into 10 groups: control group (corn oil), NP groups [30, 90, 270 mg/kg], OP groups [40, 120, 360 mg/kg] and NO groups [(mixed with the corresponding NP, OP alone exposed low, medium and high dose according to the natural environment exists NP:OP = 4:1]. The rats were orally administered every other day for 30 days. The neurobehaviors of rats were evaluated by open-field test (OFT) and elevated plus-maze test (EPM), and the concentrations of 5-HT, monoamine oxidase (MAOA), serotonin transporter (SERT), vesicular monoamine transporter 2 (VAMT2), 5-hydroxytryptamine 1A (5-HT 1A ), 5-hydroxytryptamine 2A (5-HT 2A )，and 5-hydroxytryptamine 2C (5-HT 2C ) in the rat prefrontal cortex were analyzed by ELISA . OFT and EPM tests showed that NP or/and OP exposure induced anxiety-related behaviors in rats. 5-HT levels were significantly increased compared with the control group. The levels of MAOA, SERT, VAMT2, 5-HT 1A , 5-HT 2A, and 5-HT 2C in the prefrontal cortex reduced in different degrees by high-doses NP or/and OP exposure. In summary, NP or/and OP exposure might cause anxiety-related behaviors in rats through regulating neurotransmitter 5-HT levels by altering the expression of 5-HT decomposition enzyme MAOA, transporters SERT and VMAT2, and 5-HT receptors 5-HT 1A , 5-HT 2A, and 5-HT 2C .
研究领域:
毒理学
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神经科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Yi-Kao Hu,Ayzohra Ablat,Xiao-Lin Bai,Xiang Yin,Chao Ma,Xun Liao
Lycium ruthenicum Murr is an important edible and medicinal plant widely cultivated in Northwest China. Our previous study found that its fruit possessed rich polyphenols exhibiting significant neuroprotective effects, inspiring us to investigate the active constituents of its rootstocks, which have never been reported. In this study, we rapidly identified four phenolamides (1–4) possessing monoamine oxidase B (MAO-B) inhibitory effect by using MAO-B-functionalized cellulose filter paper-based ligand fishing, and their structures were elucidated with NMR and MS. Compounds 1, 2, and 4 exhibited moderate inhibitory effect on MAO-B with IC50 values of 71.44 ± 1.81, 28.97 ± 1.20, and 25.58 ± 0.74 μM, respectively, and enzyme kinetic analysis, molecular docking, and molecular dynamics further revealed the inhibition mechanism. All four phenolamides displayed a neuroprotective effect against 1-methyl-4-phenylpyridinium -induced SH-SY5Y cells model, among which 2 and 4 significantly reduced intracellular MAO-B activity and improved the ATP content. Western blot revealed that compounds 2 and 4 alleviated the oxidative stress through up-regulation of the expression of Nrf2 and HO-1 protein, promoting mitophagy via activating the PINK1/Parkin pathway. Moreover, compounds 2 and 4 extended the lifespan, dopamine and ATP levels, locomotor behavior, and olfactory ability of the PINK1B9 flies, a drosophila model of Parkinson’s disease. Meanwhile, compounds 1–4 showed potent antioxidative activities on DPPH, ABTS+, and PTIO radical scavenging assays. These results revealed the health-promoting effects of the rootstocks in preventing and treating neurodegenerative diseases, laying the scientific foundation for the exploitation of this plant byproduct for the development of functional food formulations and pharmaceutical products.
研究领域:
植物化学
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神经科学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
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2024
·
IF 4.1
Xuchun Qiu,Zhen Su,Jiarui Gao,Yiming Cui,Kejun Dong,Kun Chen,Ru-Jin Zhao,Songmei Wang,Tao Wu,Yanhong Shi
Thimerosal (THI) is the most widely used form of organic mercury in pharmaceutical and personal care products, and has become a major source of ethylmercury pollution in aquatic ecosystems. However, knowledge about its potential risk to aquatic species is limited. In this study, zebrafish were exposed to THI for 7 days, and variations in their behavioral traits, brain monoaminergic neurotransmitter contents, and related gene expression were investigated. After the 7-day exposure, THI reduced locomotor activity and thigmotaxis in males but not females. Exposure to THI increased the social interaction between females but decreased that between males. The THI exposure also significantly reduced the serotonin (5-HT), 5-hydroxyindoleacetic acid, dopamine (DA), and 3,4-dihydroxyphenylacetic acid contents in the brain of males, but only significantly decreased the DA content in females. Correlation analysis revealed that the neurochemical alterations in the brain of zebrafish play critical roles in the behavioral abnormalities induced by THI exposure. Moreover, THI also significantly altered the expression of some genes associated with the synthesis, metabolism, and receptor binding of 5-HT and DA in the brain of zebrafish. The differences in these gene expressions between female and male zebrafish exposed to THI seem to be an important mechanism underlying their sex-specific responses to this chemical. This is the first report on the sex-specific effects of THI on behaviors and brain monoaminergic neurotransmitter contents in zebrafish, which can further improve our understanding of its toxic effects on teleost.
研究领域:
环境毒理学
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神经生物学
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生态学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Yuanyuan Zhang,Jing Wang,Wanxiang Yi,Aliya Tiemuer,Hui Yu,Yi Liu,Dan Su
Monoamine oxidase B (MAO-B) activity has been observed abnormally elevated in Parkinson’s disease patients and can be used as a potential biomarker. Herein, we report an activatable frequency upconversion luminescence (FUCL) MAO-B nano-probe (NIR-MNP) capable of highly specific and real-time MAO-B imaging in biological samples. The turn-on FUCL effect is beneficial to obtaining high-quality imaging and penetrating tissue to achieve in vivo imaging, making it stand out from the existing probes. Moreover, we used NIR-MNP to confirm the role of MAO-B inhibitor in delaying Parkinson’s disease (PD) progression in mouse PD models through bio-imaging technique in vivo. Finally, our study may offer a visual way help researchers to gain further insight into the involvement of MAO-B in PD onset and progression in animals.
研究领域:
医学
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神经科学
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生物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Qilin Yang,Shuangping Liu,Aibao Sun,Xin Li,Tiantian Liu,Xiao Han,Jian Mao
Amine oxidases have strong capabilities for the degradation of biogenic amines (BAs) in fermented foods. However, their application is limited by substrate specificity, and high concentrations of ethanol and salt can hinder their effectiveness. This study presents a novel approach utilizing comparative genomics, protein clustering analysis, and full homology modeling to identify three amine oxidases: KCYOBN from the salt-resistant Bacillus subtilis, and LYYOBN1 and LYYOBN2 from the ethanol-resistant Bacillus cereus. These enzymes are highly similar in structure and exhibit broad substrate specificities. KCYOBN maintains over 84% relative activity at 20% (w/v) NaCl, while LYYOBN1 and LYYOBN2 retain over 32 and 21% relative activity at 25% vol ethanol. Variations in key residues are one of the reasons for the differences in tolerance. In fermented foods, KCYOBN degraded 45.97% of the total BAs in cooking wine and 38.33% in fish sauce. LYYOBN1 achieved the highest degradation rate of 32.93% in huangjiu. LYYOBN2 exhibited degradation rates of 30.00% in soy sauce and 35.14% in wine with no significant impact on flavor compounds. The significance of this work lies in the identification of the novel salt-resistant KCYOBN and ethanol-resistant LYYOBN1 and LYYOBN2 through this new method, which can simultaneously degrade multiple BAs and have a broad application potential.
研究领域:
微生物学
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食品科学
·
酶工程
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DOI
PubMed
高影响因子
Genes & Diseases
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2018
·
IF 0
Dan Huang,Lu Zhang,Jun-qing Yang,Ying Luo,Ting Cui,Ting-ting Du,Xin-hui Jiang
Inflammation drives the development of depression and may affect neurotransmitters and thus neurocircuits increase the risk of depression. To investigate the influence of inhibition of inflammatory pathways on the biogenic amine neurotransmitters metabolism in depressive rats, sertraline, and meloxicam, the inhibitors of arachidonic acid - cyclooxygenase-2/lipoxygenase (AA-COX-2/5-LO) pathways, were given to depressive rats. After the development of depression model by chronic unpredictable mild stress (CUMS) for 6 weeks, Successful modeling rats were selected and randomly divided into CUMS group and medication administration group. After given medicine, The biogenic amine neurotransmitters in rat cortex and hippocampus were measured by high-performance liquid chromatography equipped with an electrochemical detector (HPLC-ECD). Compared with the normal group, the concentration of norepinephrine (NE) significantly decreased and the concentrations of Tyrosine (Tyr), Tryptophan (Trp), 3,4-dihydroxyphenyl acetic acid (DOPAC), 3-methoxy-4-hydroxyphenylglycol (MHPG), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) significantly increased in the CUMS group. Sertraline significantly inhibited the elevation of 5-HIAA. Meloxicam inhibited the decrease of NE level in CUMS-induced rat and the increase of Trp, MHPG, and 5-HIAA level in a dose-dependent manner. Caffeic acid inhibited the decrease of NE and the increase of Trp and MHPG in a dose-dependent manner. The inhibition of AA-COX-2/5-LO pathways can improve the behaviors of depression rats and suppress CUMS-induced changes in biogenic amines. Compared with the single-dose lipoxygenase (5-LO) or Cyclooxygenase-2 (COX-2) inhibitor, the combination treatment with meloxicam 1 mg/kg and caffeic acid 10 mg/kg have no significant improvement in CUMS-induced depression behavior and the level of cortical monoamine neurotransmitters and their metabolites.
研究领域:
神经科学
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药理学
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精神病学
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产品关联
DOI
PubMed
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 13
·2394-2405
·
IF 3.614
Xu-Wei Qi,Yi-Ming Liu,Yi-Kao Hu,Hao Yuan,Emmanuel Ayodeji Ayeni,Xun Liao
A novel strategy of performing ligand fishing with enzyme-modified open tubular microchannel was proposed for screening bioactive components present in medicinal plants. Monoamine oxidase B was immobilized onto the surface of the microchannel for the first time to specifically extract its ligands when the plant's extracts solution flows through the channel. The thermal and the storage stability of immobilized monoamine oxidase B were significantly enhanced after immobilization. Crocin I and Ⅱ were extracted from Crocus sativus , and tiliroside was extracted from Edgeworthia gardneri . All the three compounds were inhibitors of the enzyme with the half-maximal inhibitory concentration values of 26.70 ± 0.91, 19.88 ± 2.78, and 15.65 ± 0.85 μM, respectively. The enzyme inhibition kinetics and molecular docking were investigated. This is the first report on the inhibitory effects of tiliroside and crocin Ⅱ. The novel ligand fishing method proposed in this work possesses advantages of rapidness, high efficiency, and tiny sample consumption compared to routine ligand fishing, with promising potential for screening active natural products in complex mixtures.
研究领域:
生物化学
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药理学
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植物化学
✓ 已验证
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2025
·
IF 4.2
Yujing Zhou,Jue Xu,Xiankang Fan,Qiang Xia,Changyu Zhou,Yangyang Hu,Hongbing Yan,Yangying Sun,Daodong Pan
In this study, the effect of pulsed electric field (PEF) pretreatment synergistic mixed bacterial agent ( Lactiplantibacillus plantarum PDD-1 and Lactococcus lactis subsp. lactis JCM5805) fermentation on the flavor and quality of air-dried goose meat and its molecular mechanism were investigated. The results showed that PEF combined with the mixed bacteria fermentation increased activity of monoamine oxidase (232.57 U/mg protein), decreased TBARS (0.3 mg/kg) and TVB-N (15.75 mg/100 g) content, and the hardness and chewiness in air-dried goose. The improvement in flavor due to the synergistic fermentation following PEF pretreatment was primarily attributed to increased levels of aromatic compounds such as Heptanal (green, sweet), (Z)-3-Hexenyl acetate (green fruity), 3-(methylthio)-propanol (onion, bouillion-like note), alongside decreased levels of off-flavor compounds like methyl phenylacetate and 5-methyl-2-acetylfuran, as determined by the e-nose and GC-IMS. Colony counts and high-throughput sequencing demonstrated that Lactococcus and Staphylococcu s predominated as the primary bacterial species, with different bioma onion, bouillion-like note rkers present in different air-dried goose groups. This is of strategic importance to develop the unique flavor and quality guarantee of air-dried goose.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）