Foods
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2023
·Vol. 12
·期 2
·404
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IF 5.2
Xingguang Peng,Hongsheng Liu,Xuying Li,Huaibin Wang,Kejia Zhang,Shuangqi Li,Xianyang Bao,Wei Zou,Wenwen Yu
In vitro digestion methods that can accurately predict the estimated GI (eGI) values of complex carbohydrate foods, including biscuits, are worth exploring. In the current study, standard commercial biscuits with varied clinical GI values between 9~30 were digested using both the INFOGEST and single-enzyme digestion protocols. The digestion kinetic parameters were acquired through mathematical fitting by mathematical kinetics models. The results showed that compared with the INFOGEST protocol, theAUR180deduced from digesting using either porcine pancreatin or α-amylase showed the best potential in predicting the eGI values. Accordingly, mathematical equations were established based on the relations between theAUR180and the GI values. When digesting using porcine pancreatin,GI=1.834 + 0.009×AUCR180(R2=0.952), and when digesting using only α-amylase,GI=6.101 + 0.009×AUCR180(R2=0.902). TheAUR180represents the area under the curve of the reducing-sugar content normalized to the total carbohydrates versus the digestion time in 180 min. The in vitro method presented enabled the rapid and accurate prediction of the eGI values of biscuits, and the validity of the formula was verified by another batch of biscuits with a known GI, and the error rate of most samples was less than 30%.Keywords:biscuits;in vitro digestion;INFOGEST;single enzyme;glycemic index
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Xin Li,Pengfei Lv,Yixin Yao,Quan Feng,Alfred Mensah,Dawei Li,Qufu Wei
Enzymatic biofuel cell (EBFC) uses enzymes to convert the chemical energy of renewable biofuels into electrical energy, which shows great promise in the continuous power supply of implantable devices. Meanwhile, integrating carbon nanotubes (CNTs) with high conductivity and superior structural properties of bacterial cellulose (BC) as backbone to construct CNTs-BC based nanocomposites has proven to be an effective strategy. Herein, a highly flexible electrode of amidoxime-modified BC (AOBC)/carboxylated multi-walled CNTs (c-MWCNTs) were prepared by vacuum filtration with excellent laccase (LAC) immobilization. Subsequently, we developed a novel EBFC that intramolecular electron transfer was achieved by an alternative pathway for generating electricity within a single enzyme molecule of LAC. Sufficient power was efficiently extracted from unconventional fuels in single-LAC biofuel cells, including persistent pollutants (e.g., bisphenol A, hydroquinone and pyrocatechol). The open circuit voltage generated by EBFC was 0.14 V and the power density reached 1.897 W m −3 . This novel single-enzymatic biofuel cell exhibited a potential for recycling of waste water as fuel.
研究领域:
生物技术
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
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2015
·
IF 3.545
Mengzhen Dai,Ting Huang,Long Chao,Qingji Xie,Yueming Tan,Chao Chen,Wenhua Meng
Horseradish peroxidase (HRP)-catalyzed polymerization of l -DOPA (vs. dopamine) in the presence of H 2 O 2 (and uricase (UOx)) was exploited to immobilize mono-/bi-enzymes for hydroquinone-mediated amperometric biosensing of H 2 O 2 and uric acid (UA). The relevant polymeric biocomposites (PBCs) were prepared in phosphate buffer solution containing HRP and l -DOPA (or plus UOx) after adding H 2 O 2 . The mono-/bi-enzyme amperometric biosensors were prepared simply by casting some of the PBCs on Au-plated Au (Au plate /Au) electrodes, followed by coating with an outer-layer chitosan (CS) film for each. UV–vis spectrophotometry , scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were used for film characterization and/or process monitoring. The HRP immobilized by enzyme catalysis well preserved its bioactivity, as confirmed by UV–vis spectrophotometry . Under optimized conditions, the monoenzyme CS/HRP-poly( l -DOPA) (PD)/Au plate /Au electrode potentiostated at −0.1 V responded linearly to H 2 O 2 concentration from 0.001 to 1.25 mM with a sensitivity of 700 μA mM −1 cm −2 and a limit of detection (LOD) of 0.1 μM, and the bienzyme CS/UOx–HRP–PD/Au plate /Au electrode at −0.1 V responded linearly to UA concentration from 0.001 to 0.4 mM with a sensitivity of 349 μA mM −1 cm −2 and a LOD of 0.1 μM. The mono-/bi-enzyme biosensors based on biosynthesized PD performed better than many reported analogues and those based on similarly biosynthesized polydopamine.
研究领域:
生物化学
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分析化学
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生物医学工程
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DOI
PubMed
高影响因子
Science Advances
·
2022
·Vol. 8
·期 18
·
IF 14.957
Xinyu Wang,Jicong Zhang,Ke Li,Bolin An,Yanyi Wang,Chao Zhong
There is an increasing trend of combining living cells with inorganic semiconductors to construct semi-artificial photosynthesis systems. Creating a robust and benign bio-abiotic interface is key to the success of such solar-to-chemical conversions but often faces a variety of challenges, including biocompatibility and the susceptibility of cell membrane to high-energy damage arising from direct interfacial contact. Here, we report living mineralized biofilms as an ultrastable and biocompatible bio-abiotic interface to implement single enzyme to whole-cell photocatalytic applications. These photocatalyst-mineralized biofilms exhibited efficient photoelectrical responses and were further exploited for diverse photocatalytic reaction systems including a whole-cell photocatalytic CO2 reduction system enabled by the same biofilm-producing strain. Segregated from the extracellularly mineralized semiconductors, the bacteria remained alive even after 5 cycles of photocatalytic NADH regeneration reactions, and the biofilms could be easily regenerated. Our work thus demonstrates the construction of biocompatible interfaces using biofilm matrices and establishes proof of concept for future sustainable photocatalytic applications.
研究领域:
生物技术
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材料科学
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环境科学
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光化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）