COLLOIDS AND SURFACES B-BIOINTERFACES
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2017
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IF 3.887
Yibang Zhang,Weibeng Ng,Jianguo Hu,Salma Saleh Mussa,Yanru Ge,Huaxi Xu
The primary objective of this study was to develop, evaluate and compare the effectiveness and stability of ethosomal carbomer gels in different solvents. The optimal ethosomal formulation was isolated to create ethosomal gels using carbomer in either pure water (water gel) or PBS containing 30% ethanol (PBS gel). In vitro release of the ethosomal gels were tested using Franz apparatus on hydrophilic and hydrophobic artificial membranes. In vitro stability of two ethosomal gels was systematically evaluated. Transdermal antigen delivery of ethosomal gel was finally performed on the skin of hair removal mice. Both solvent and concentration effects on the in vitro release performance of ethosomal gel of carbomer have been confirmed. Penetration depth has been found to be dependent on the nature of the membranes such that penetration rate is higher in the hydrophobic membrane than the hydrophilic ones. Furthermore, in vitro stability test indicated that ethosomal PBS gel was more stable than ethosomal water gel. In vivo immunoassay confirmed that the ethosomal PBS gel could deliver the antigenic molecules into the skin of mice and stimulate specific IgG secretion. Using the same solvent for lipid vesicular formulation when making polymeric hydrogel may help to provide a more conducive environment for lipid vesicles and hence enhance their roles in transdermal antigen delivery.
研究领域:
药剂学
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免疫学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2022
·
IF 6.51
Xier Pan,Yushu Li,Weilin Pang,Yaqi Xue,Zhuxian Wang,Cuiping Jiang,Chunyan Shen,Qiang Liu,Li Liu
Poly(vinyl alcohol) (PVA) and carbomer were used as the hydrogel system to fabricate glabridin-loaded hydrogel-forming microneedles (HFMNs) by chemical cross-linking (CCMNs) and physical cross-linking (PCMNs). The properties and drug permeation effects of glabridin-loaded HFMNs with different methods were compared. They both owned excellent shapes, mechanical and insertion properties. PCMNs showed collapsed shapes during swelling due to the low cross-linking rate and high porosity, which probably results in resealing of skin pores during transdermal drug delivery. However, CCMNs could rapidly swell within 2 h with slightly bending. The infrared spectra indicate that CCMNs and PCMNs might form the hydrogel network by generating hydrogen and covalent bonds, respectively. The in vitro release studies showed that cumulative permeation amount within 24 h (1654 μg/cm 2 ) of CCMNs significantly higher than that (372 μg/cm 2 ) achieved by PCMNs and that (118 μg/cm 2 ) achieved by glabridin-loaded gel. The skin barrier recovery test suggests the desirable security of both microneedles (MNs), notwithstanding the presence of mild erythema in the mouse skin applied CCMNs. These results indicate that CCMNs were more desirable for glabridin delivery using PVA and carbomer as a skeleton of the hydrogel network.
研究领域:
药剂学
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生物材料
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皮肤科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2017
·
IF 3.649
Jiao Yu,Kawai Wan,Xun Sun
The skin is an important site for local or systemic application of drugs. However, most of the drugs have poor permeability through the skin’s outermost layer, stratum corneum. The aim of this study was to develop a method to enable transdermal delivery of morin (3, 5, 7, 2, 4-pentahydroxyflavone), which is a poorly water-soluble drug with anti-inflammatory properties obtained from natural products. Morin phospholipid complex (MPC) was prepared and then loaded in Carbopol 940 hydrogel (MPC-gel), which can significantly increase the transdermal flux of morin based on the in vitro skin penetration data presented in this paper. To further enhance permeation, different compositions of penetration enhancers were dispersed in the gel and screened. After applied onto the mouse skin, MPC-gel showed apparent reduction of ear swelling in 2, 4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis (ACD). Further determination of cytokines levels, histopathological analysis and T lymphocytes proliferation indicates that the MPC-gel is potent enough to reduce the inflammatory response mediated by the DNFB in ACD mice model. Collectively, we anticipate that such an approach may provide a new treatment for topical ACD.
研究领域:
药剂学
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免疫学
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皮肤病学
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产品关联
DOI
PubMed
高影响因子
Materials Today Communications
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2025
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IF 4.5
Liang Sun,Baiqiang Luo,Zequn Sun,Xiuyan Ren,Jiajun Xu
With the extensive development and application of hydrogel strain sensors, people have begun to pay attention to hydrogel strain sensors with water-resistant properties and multi-dimensional complex shapes, such as underwater sensors and printable sensors. Here, we successfully designed a novel multifunctional hydrogel, which was not only an anti-swelling hydrogel by double hydrophobic crosslinking for underwater strain sensor, but also carbomer-modified printable hydrogels for multi-dimensional complex strain sensors. The structure, mechanical, anti-swelling and sensing properties of the modified polyacrylamide hydrogel were also systematically analyzed. The experimental results demonstrated that the hydrogel exhibited excellent anti-swelling properties, with a maximum stress of up to 570 kPa after swelling for 48 h. Meanwhile, by introducing carbomer, the hydrogel was imbued with Bingham fluid characteristics, allowing it to be printed into various strain sensors and still exhibited high deformation sensitivity even after swelling for 48 h. Notably, the aforementioned hydrogel was also successfully applied to underwater signal transmission, where it not only monitored the swimming movements of a small wooden figure, as swinging arms and bending knees, but also measured Morse code through finger movements and sent out emergency signals. Collectively, the study would hopefully provide a novel strategy for underwater signal transmission and sensing rescue.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
ACS Applied Engineering Materials
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2023
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IF 0
Yu-cheng Zhang,Ya-qi Xue,Takayo Ogawa,Satoshi Wada,Jin-Ye Wang
Manufacturing biocompatible materials with higher-order structure has great significance because they can mimic the extracellular medium of the human organism and are a novel strategy for tissue regeneration. In this study, a device with stiffness-gradient characteristics based on two biocompatible materials, alginate with presolidification and photocurable acrylamide-containing supporting bath, was designed and constructed by the 3D printing technique. The presolidification can avoid rapid diffusion of alginate in aqueous solutions, improve mechanical properties without the introduction of heterogeneous gel precursor, and endow gradient stiffness by the controlled diffusion of calcium ions. Besides, a photocurable supporting bath was combined to manufacture a device with a dual-gradient structure by a 4-step procedure, including 3D printing, removal of the inner hydrogel, solidification of alginate, and curing of the supporting bath. A cylinder-like container was manufactured as the template, and the wall of the resultant container with two types of gradient structures showed parabola-like stiffness changes (open upward), resulting from calcium ion diffusion-controlled gradient solidification and alginate diffusion-controlled gradient photocuring. Moreover, the resultant device exhibited lower cytotoxicity to both adherent and suspension cells than containers manufactured with alginate. Because of the high water uptake of the photocured supporting bath, the removal of toxic metabolic products together with cell culture medium from the container leads to better cell compatibility. This diffusion-controlled device is also applicable to other additive manufacturers with biomedical significance.
研究领域:
生物材料
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组织工程
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增材制造
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细胞培养
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DOI
Marine Drugs
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2021
·Vol. 19
·期 6
·344
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IF 5.118
Qiong Xiao,Guo Chen,Yong-Hui Zhang,Fu-Quan Chen,Hui-Fen Weng,An-Feng Xiao
Some commonly used surfactants in cosmetic products raise concerns due to their skin-irritating effects and environmental contamination. Multifunctional, high-performance polymers are good alternatives to overcome these problems. In this study, agarose stearate (AS) with emulsifying, thickening, and gel properties was synthesized. Surfactant-free cosmetic formulations were successfully prepared from AS and carbomer940(CBM940) mixed systems. The correlation of rheological parameter with skin feeling was determined to study the usability of the mixed systems in cosmetics. Based on rheological analysis, the surfactant-free cosmetic cream (SFC) stabilized by AS-carbomer940showed shear-thinning behavior and strongly synergistic action. The SFC exhibited a gel-like behavior and had rheological properties similar to commercial cosmetic creams. Scanning electron microscope images proved that the AS-CBM940network played an important role in SFC’s stability. Oil content could reinforce the elastic characteristics of the AS-CBM940matrix. The SFCs showed a good appearance and sensation during and after rubbing into skin. The knowledge gained from this study may be useful for designing surfactant-free cosmetic cream with rheological properties that can be tailored for particular commercial cosmetic applications. They may also be useful for producing medicine products with highly viscous or gel-like textures, such as some ointments and wound dressings.Keywords:agarose stearate;carbomer940;surfactant-free cosmetics;rheological behavior;sensory evaluation
研究领域:
化妆品科学
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材料科学
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生物学
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产品关联
DOI
PubMed
高影响因子
Materials Today Communications
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2022
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IF 3.662
Yibang Zhang,Fangyin Cao,Aftab Ullah
Transcutaneous immunization is becoming an attractive non-invasive vaccination strategy, though it is still challenging. This study evaluates the potential of transcutaneous delivery of antigens in mice through niosomal and elastic niosomal carbomer hydrogels. Ovalbumin was encapsulated into niosomes prepared by Span 80/cholesterol/stearylamine through reverse-phase evaporation. Ethanol was added into niosomal mixtures for elastic niosomes formation, then embedded in carbopol 934 hydrogel. Encapsulation efficiency and other physiochemical properties of the prepared niosomal vesicles such as particle size, stability, in vitro release and cytotoxicity were determined. In vivo immunizations of niosomal and elastic niosomal gel were carried out in mice. The optimal niosomal formulation loads 48.6 ± 5.2% ovalbumin with a vesicle size below 300 nm. A simple BCA method was used to determine the encapsulation efficiency of niosome. The cumulative permeation of proteins of niosomal gel was 15~20%. Both gels were stable at room temperature; however, elastic niosomal gel deteriorated at 60 ℃. Compared to free antigen, the topical administration of niosomal or elastic niosomal gel to mice displayed significant IgG titers. From the findings, compared to elastic niosomal gel, the niosomal hydrogel platform exhibited enhanced antigen delivery. Therefore, niosomal hydrogel may be considered a promising transcutaneous antigen delivery carrier.
研究领域:
药剂学
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免疫学
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药物递送系统
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产品关联
DOI
高影响因子
Materials Today Bio
·
2022
·
IF 10.761
Zesong Ruan,Chunlei Zhang,Tingwang Shi,Zhiyuan Luo,Yuna Zhang,Zanxia Cao,Rentai Huang,Yunfeng Chen,Daxiang Cui
Restoring skin integrity after wound infection remains a tougher health challenge due to the uncontrolled antibiotic-resistant pathogens caused by antibiotic abuse. Herein, an injectable hydrogel with dual antibacterial and anti-inflammatory activities composed of gold nanoclusters (GNCs) and carbomer (CBM) is developed for wound dressing to overcome multidrug-resistant infection. Firstly, both experimental investigations and molecular dynamics simulation validate the protonation state of 6-mercaptohexanoic acid (MHA) ligands play an important role in its antibacterial action of GNCs. The self-organizing GNCs-CBM composite hydrogel is then spontaneously cross-linked by the dimeric hydrogen bonds (H-bonds) between the MHA ligands and the acrylic acid groups of CBM. Benefitting from the dimeric H-bonds, the hydrogel becomes thickening enough as an ideal wound dressing and the GNCs exist in the hydrogel with a high protonation level that contributes to the enhanced bactericidal function. In all, by combining bactericidal and immunomodulatory actions, the GNCs-CBM hydrogel demonstrated excellent synergy in accelerating wound healing in animal infection models. Hence, the dimeric H-bonds strengthening strategy makes the GNCs-CBM hydrogel hold great potential as a safe and effective dressing for treating infected wounds.
研究领域:
材料科学
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纳米技术
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生物医学工程
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药剂学
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伤口愈合
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感染性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 5
·1-14
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IF 3.246
Ma Qiuyan,Zhang Jing,Lu Bohong,Lin Huaqing,Sarkar Rajib,Wu Tao,Li Xuee
This research aimed to develop a novel drug delivery system to improve treatment of skin disorders. The system is comprised of a Carbopol 980-based nanoemulgel (NE-gel) containing a desonide (DES; 0.05%, w/w) nanoemulsion (NE), which has a small particle size, high encapsulation efficiency, good thermodynamic stability, good permeation ability, and high skin retention. DES-loaded NE (DES-NE) was prepared by high-pressure homogenization. The developed formulation was characterized by differential scanning calorimetry (DSC), X-ray diffraction, drug release, skin permeation, and drug retention. DES in vitro release and skin permeation studies with different formulations of artificial membrane and rat abdominal skin were performed with the Franz diffusion cell system. Confocal laser scanning microscopy (CLSM) was used to detect the localization and permeation pathways of drugs in the skin. Compared with commercially available gel (CA-gel) and NE, the NE-gel release process conformed to the Higuchi release model ( R 2 = 0.9813). NE-gel prolonged the drug release time and allowed for reduced administration dose and frequency. The unit cumulative permeation of NE and NE-gel through the skin for 12 h was 63.13 ± 2.78 and 42.53 ± 2.06 μg/cm 2 , respectively, values significantly higher ( p < 0.01) than that of the CA-gel (30.65 ± 1.25 μg/cm 2 ) and CA-cream (15.21 ± 0.97 μg/cm 2 ). The DES-NE and DES NE-gel skin drug retention was significantly higher than commercially available formulations ( p < 0.01). Hence, the prepared NE-gel is a potential vehicle for improved topical DES delivery for better treatment of skin disorders.
研究领域:
药剂学
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药物递送系统
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制药技术
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药学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Biomaterials
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2023
·Vol. 14
·期 5
·278
·
IF 4.8
Chengqun Yu,Abid Naeem,Yali Liu,Yongmei Guan
Oxidants play a crucial role in the development of oxidative stress, which is linked to disease progression. Ellagic acid is an effective antioxidant with applications in the treatment and prevention of several diseases, since it neutralizes free radicals and reduces oxidative stress. However, it has limited application due to its poor solubility and oral bioavailability. Since ellagic acid is hydrophobic, it is difficult to load it directly into hydrogels for controlled release applications. Therefore, the purpose of this study was to first prepare inclusion complexes of ellagic acid (EA) with hydroxypropyl-β-cyclodextrin and then load them into carbopol-934-grafted-2-acrylamido-2-methyl-1-propane sulfonic acid (CP-g-AMPS) hydrogels for orally controlled drug delivery. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) were used to validate ellagic acid inclusion complexes and hydrogels. There was slightly higher swelling and drug release at pH 1.2 (42.20% and 92.13%) than at pH 7.4 (31.61% and 77.28%), respectively. Hydrogels had high porosity (88.90%) and biodegradation (9.2% per week in phosphate-buffered saline). Hydrogels were tested for their antioxidant properties in vitro against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Additionally, the antibacterial activity of hydrogels was demonstrated against Gram-positive bacterial strains (Staphylococcus aureusandEscherichia coli) and Gram-negative bacterial strains (Pseudomonas aeruginosa).Keywords:hydrogels;polyphenol;phytoconstituents;inclusion complexation;anti-oxidants;bioavailability
研究领域:
药学
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材料科学
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生物技术
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化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Anan Guo,Lvqing Tang,Bing Yang,Niling Xie,Yandong Cui,Wen Sun,Yuan Li,Xiangting Li,Yang Wu,Yingshuai Liu
Adjuvants including aluminum adjuvant (Alum) and oil-water emulsion have been widely used in inactivated pseudorabies virus (PRV) vaccines to improve their performance, however, they are not sufficient to protect from PRV infection because of the weak immune response and poor Th1-type immune response. Divalent manganese ion (Mn 2+ ) has been reported to increase the cellular immune response significantly. In this work, a xanthan gum and carbomer-dispersed Mn 2+ -loaded tannic acid-polyethylene glycol (TPMnXC) nanoparticle colloid is developed and used as an adjuvant to improve the performance of the inactivated PRV vaccine. The good in vitro and in vivo biocompatibility of the developed TPMnXC colloid has been confirmed by the cell viability assay, erythrocyte hemolysis, blood routine analysis, and histological analysis of mouse organs and injection site. The TPMnXC-adjuvanted inactivated PRV vaccine (TPMnXC@PRV) significantly promotes higher and more balanced immune responses indicating with an increased specific total IgG antibody and IgG2a/IgG1 ratio, efficient splenocytes proliferation, and elevated Th1- and Th2-type cytokine secretion than those of control groups. Wild PRV challenge experiment is performed using mice as a model animal, achieving a protection rate of up to 86.67 %, which is much higher than those observed from the commercial Alum. This work not only demonstrates the high potentiality of TPMnXC in practical applications but also provides a new way to develop the Mn 2+ -loaded nanoadjuvant for veterinary vaccines.
研究领域:
兽医学
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免疫学
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纳米技术
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病毒学
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产品关联
DOI
PubMed
高影响因子
Burns & Trauma
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2021
·
IF 5.099
Wang Huanhuan,Liu Yang,Cai Kun,Zhang Bin,Tang Shijie,Zhang Wancong,Liu Wenhua
BackgroundPolysaccharide-based hydrogels have been developed for many years to treat burn wounds. Essential oils extracted from aromatic plants generally exhibit superior biological activity, especially antibacterial properties. Studies have shown that antibacterial hydrogels mixed with essential oils have great potential for burn wound healing. This study aimed to develop an antibacterial polysaccharide-based hydrogel with essential oil for burn skin repair.MethodsEucalyptus essential oil (EEO), ginger essential oil (GEO) and cumin essential oil (CEO) were employed for the preparation of effective antibacterial hydrogels physically crosslinked by carboxymethyl chitosan (CMC) and carbomer 940 (CBM). Composite hydrogels were prepared and characterized using antimicrobial activity studies, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, gas chromatography-mass spectrometery, rheological analysis, viscosity, swelling, water loss rate and water vapor transmission rate studies. In addition, the biocompatibility of hydrogels was evaluated in vivo by cytotoxicity and cell migration assays and the burn healing ability of hydrogels was tested in vivo using burn-induced wounds in mice.ResultsThe different essential oils exhibited different mixing abilities with the hydrogel matrix (CMC and CBM), which caused varying levels of reduction in essential oil hydrogel viscosity, swelling and water vapor transmission. Among the developed hydrogels, the CBM/CMC/EEO hydrogel exhibited optimal antibacterial activities of 46.26 ± 2.22% and 63.05 ± 0.99% against Staphylococcus aureus and Escherichia coli, respectively, along with cell viability (>92.37%) and migration activity. Furthermore, the CBM/CMC/EEO hydrogel accelerated wound healing in mouse burn models by promoting the recovery of dermis and epidermis as observed using a hematoxylin–eosin and Masson’s trichrome staining assay. The findings from an enzyme-linked immunosorbent assay demonstrated that the CBM/CMC/EEO hydrogel could repair wounds through interleukin-6 and tumor necrosis factor-α downregulation and transforming growth factor-β, vascular endothelial growth factor (VEGF) and epidermal growth factor upregulation.ConclusionsThis study successfully prepared a porous CBM/CMC/EEO hydrogel with high antibacterial activity, favorable swelling, optimal rheological properties, superior water retention and water vapor transmission performance and a significant effect on skin repair in vitro and in vivo. The results indicate that the CBM/CMC/EEO hydrogel has the potential for use as a promising burn dressing material for skin burn repair.
研究领域:
材料科学
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生物医学工程
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药学
·
伤口愈合
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生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）