FOOD AND AGRICULTURAL IMMUNOLOGY
·
2020
·
IF 0
Guanqiong Na,Xiaofei Hu,Yaning Sun,Guangxu Xing,Yunrui Xing,Gaiping Zhang
Carprofen (CAR) is a non-steroidal anti-inflammatory drug commonly used to treat various musculoskeletal diseases and infections in food animals. While CAR residues in food animal tissues caused by veterinary medicine abuse are a potential danger to human health. In this study, a high-affinity monoclonal antibody (mAb) against CAR was prepared, and then an immunochromatographic strip for rapidly screening CAR in bovine muscle was developed to help enforce regulatory compliance. The visual detection limit (cut-off value) by the naked eye of the fabricated test strip was 12.5 ng g−1. Scanning by the strip reader, the IC50 of the test strip was calculated as 1.743 ng g−1, and the limit of detection (LOD, IC10) was 0.283 ng g−1. The proposed method provided an efficient tool for monitoring CAR in bovine muscle. Meantime, our work suggested great potential for the analysis of CAR residues in other food matrixes.
研究领域:
兽医学
·
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
CHEMICAL RESEARCH IN TOXICOLOGY
·
2021
·
IF 3.739
Jiaxing He,Mingyue Liu,Lingfeng Yin,Ziqi Deng,Junhong Pang,Haoxian He,Li Dang,Ming-De Li
Carprofen (CP), one kind of a nonsteroidal anti-inflammatory drug, exhibits phototoxic side effects in physiology, while its phototoxic mechanism is ambiguous. To uncover CP’s photophysical and photochemical reaction processes, femtosecond to nanosecond transient absorption spectroscopies were employed to directly detect excited states and transient intermediates of CP upon UV irradiation in pure acetonitrile (MeCN), MeCN/water 1:1, and acid/alkaline buffer solutions. The transient absorption data together with DFT calculations were integrated to elucidate mechanisms for photochemical reactions of CP in different solutions. The associated photophysical and photochemical reaction pathways are dependent on various solution environments. In a pure MeCN solvent, CP is excited to a singlet state (S1) and rapidly interacts with the solvent to proceed solvent rearrangement (SR). It then undergoes vibrational cooling (VC) and proceeds intersystem crossing (ISC) to produce the lowest triplet state (3CP). 3CP finally decays to the ground state. While in a MeCN/water 1:1 solution, deprotonated S1 of CP experiences SR and VC processes, and then it is promoted to a deprotonated triplet state (3CP–). 3CP– undergoes the parallel reactions: dechlorination to a phenyl radical (2CP–) and decarboxylation to a T1 anion (3CP–(de-CO2)). Finally, both intermediates produce the radical anion species 2CP–(de-CO2). In a pH = 7.4 (MeCN/PBS 1:1) solution, 3CP– can be converted into 2CP–(de-CO2) more quickly. Interestingly, we found that the dechlorination step can be promoted in an alkaline solution. Phenyl and chlorine radicals produced in an aqueous solution may be the root cause of the drug’s harmful side effects on the human body. This may be useful to guide the design of related CP drugs with minimal phototoxicity in the pharmaceutical process.
研究领域:
药理学
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光化学
·
理论化学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2023
·
IF 5.7
Florea Dumitrascu,Mino R. Caira,Speranta Avram,Catalin Buiu,Ana Maria Udrea,Ilinca Margareta Vlad,Irina Zarafu,Petre Ioniță,Diana Camelia Nuță,Marcela Popa,Mariana-Carmen Chifiriuc,Carmen Limban
Introduction One of the promising leads for the rapid discovery of alternative antimicrobial agents is to repurpose other drugs, such as nonsteroidal anti-inflammatory agents (NSAIDs) for fighting bacterial infections and antimicrobial resistance. Methods A series of new carbazole derivatives based on the readily available anti-inflammatory drug carprofen has been obtained by nitration, halogenation and N-alkylation of carprofen and its esters. The structures of these carbazole compounds were assigned by NMR and IR spectroscopy. Regioselective electrophilic substitution by nitration and halogenation at the carbazole ring was assigned from H NMR spectra. The single crystal X-ray structures of two representative derivatives obtained by dibromination of carprofen, were also determined. The total antioxidant capacity (TAC) was measured using the DPPH method. The antimicrobial activity assay was performed using quantitative methods, allowing establishment of the minimal inhibitory/bactericidal/biofilm eradication concentrations (MIC/MBC/MBEC) on Gram-positive ( Staphylococcus aureus, Enterococcus faecalis ) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa ) strains. Computational assays have been performed to assess the drug- and lead-likeness, pharmacokinetics (ADME-Tox) and pharmacogenomics profiles. Results and discussion The crystal X-ray structures of 3,8-dibromocarprofen and its methyl ester have revealed significant differences in their supramolecular assemblies. The most active antioxidant compound was 1i , bearing one chlorine and two bromine atoms, as well as the CO2Me group. Among the tested derivatives, 1h bearing one chlorine and two bromine atoms has exhibited the widest antibacterial spectrum and the most intensive inhibitory activity, especially against the Gram-positive strains, in planktonic and biofilm growth state. The compounds 1a (bearing one chlorine, one NO2 and one CO2Me group) and 1i (bearing one chlorine, two bromine atoms and a CO2Me group) exhibited the best antibiofilm activity in the case of the P. aeruginosa strain. Moreover, these compounds comply with the drug-likeness rules, have good oral bioavailability and are not carcinogenic or mutagenic. The results demonstrate that these new carbazole derivatives have a molecular profile which deserves to be explored further for the development of novel antibacterial and antibiofilm agents.
研究领域:
药理学
·
微生物学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhao-Xing Yan,Ling-Xi Zhao,Ning Liu,Na Li,Ru-Song Zhao
Non-steroidal anti-inflammatory drugs (NSAIDs) have gained significant attention as emerging pollutants and are increasingly detected in aquatic environments. Therefore, it is imperative to investigate the efficient removal of NSAIDs from water systems. In this study, a magnetic LDH with three-dimensional hollow structure (Fe 3 O 4 @ZIF-67@Co-Ni LDH) was synthesized using ZIF-67 as a template for the removal of carprofen (CPF) and flurbiprofen (FLU) from water environments. The adsorption process of CPF and FLU on Fe 3 O 4 @ZIF-67@Co-Ni LDH and the competitive adsorption between these two NSAIDs were investigated. The adsorption mechanism was explored by experimental analysis and theoretical calculation. Results demonstrated that the pseudo-second-order kinetic model and Liu isotherm model can better fit the kinetic and isotherm data, and according to the thermodynamic data analysis, the adsorption process was exothermic, spontaneous and stable. The adsorption capacity of CPF and FLU on Fe 3 O 4 @ZIF-67@Co-Ni LDH can reach 856.2 mg/g and 797.5 mg/g. Fe 3 O 4 @ZIF-67@Co-Ni LDH exhibited high adsorption capacity for CPF and FLU in actual environment water, with stronger affinity towards CPF when both NSAIDs coexisted simultaneously. The three-dimensional hollow structure enhanced the adsorption capacity of Fe 3 O 4 @ZIF-67@Co-Ni LDH, making it a promising adsorbent for NSAIDs.
研究领域:
环境科学
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水处理
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Ming Zhang,Yan Chen,Shuaiqi Feng,Yanqin He,Zhifang Liu,Ning Zhang,Qingpeng Wang
The inflammatory microenvironment is a central driver of tumor metastasis, intimately associated with the promotion of epithelial–mesenchymal transition (EMT) and immune suppression. Here, transferrin-modified carprofen platinum(IV) nanoparticles Tf-NPs@CPF2–Pt(IV) with promising antiproliferative and antimetastatic properties were developed, which activated by inhibiting inflammation, suppressing EMT, and activating immune responses besides causing DNA injury. The nanoparticles released the active ingredient CPF2–Pt(IV) in a sustained manner and offered enhanced pharmacokinetic properties compared to free CPF2–Pt(IV) in vivo. Additionally, they possessed satisfactory tumor targeting effects via the transferrin motif. Serious DNA damage was induced with the upregulation of γ-H2AX and P53, and the mitochondria-mediated apoptotic pathway Bcl-2/Bax/caspase3 was initiated. Inflammation was alleviated by inhibiting COX-2 and MMP9 and decreasing inflammatory cytokines TNF-α and IL-6. Subsequently, the EMT was reversed by inhibiting the Wnt/β-catenin pathway. Furthermore, the antitumor immunity was provoked by blocking the immune checkpoint PD-L1 and increasing CD3+ and CD8+ T lymphocytes in tumors.
研究领域:
癌症研究
·
纳米技术
·
药理学
·
免疫学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Gaoxiang Yu,Yijie Chen,Ningning Yang,Haojie Zhang,Xuzi Zhang,Yibo Geng,Jiayi Zhao,Zhuliu Chen,Chengji Dong,Lidan Lin,Jianjun Qi,Xuanlong Zhang,Xiaoqiong Jiang,Weiyang Gao,Yuepiao Cai,Xiangyang Wang,Jian Ding,Jian Xiao,Kailiang Zhou
Preventing and treating avascular necrosis at the distal end of the flaps are critical to surgery success, but current treatments are not ideal. A recent study shows that apoptotic bodies (ABs) generated near the site of apoptosis can be taken up and promote cell proliferation. The study reveals that ABs derived from fibroblast-like cells in the subcutaneous connective tissue (FSCT cells) of skin flaps promoted ischaemic flap survival. It is also found that ABs inhibited cell death and oxidative stress and promoted M1-to-M2 polarization in macrophages. Transcriptome sequencing and protein level testing demonstrated that ABs promoted ischaemic flap survival in endothelial cells and macrophages by inhibiting ferroptosis via the KEAP1-Nrf2 axis. Furthermore, microRNA (miR) sequencing data and in vitro and in vivo experiments demonstrated that ABs inhibited KEAP1 by delivering miR-339-5p to exert therapeutic effects. In conclusion, FSCT cell-derived ABs inhibited ferroptosis, promoted the macrophage M1-to-M2 transition via the miR-339-5p/KEAP1/Nrf2 axis and promoted ischaemic flap survival. These results provide a potential therapeutic strategy to promote ischaemic flap survival by administering ABs.
研究领域:
外科手术
·
细胞生物学
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分子生物学
·
组织工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2024
·
IF 3.5
Xianyao Tao,Xiaoyun Pan,Gang Zhao,Mingyu Xue,Yongjun Rui
In clinical flap practice, there are a lot of studies being done on how to promote the survival of distal random flap necrosis in the hypoxic and ischemic state. As a traditional Chinese medicine, dihydromyricetin (DHM) is crucial in preventing oxidative stress and apoptosis in a number of disorders. In this work, we examined the impact of DHM on the ability to survive of ischemia flaps and looked into its fundamental mechanism. Our results showed that DHM significantly increased the ischemic flaps' survival area, encouraged angiogenesis and blood flow, reduced oxidative stress and apoptosis, and stimulated KEAP1-Nrf2 (Kelch-like ECH-associated protein 1-nuclear factor erythroid 2-related factor) signaling pathways. Adeno-associated virus (AAV) upregulation of KEAP1 expression also negated the favorable effects of DHM on flap survival. By activating KEAP1-Nrf2 signaling pathways, DHM therapy promotes angiogenesis while reducing oxidative stress and apoptosis.
研究领域:
药理学
·
外科手术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Integrative Neuroscience
·
2024
·Vol. 23
·期 6
·116
·
IF 2.5
Fan Xv,Li-zhen Ma,Xin Li,Jin-bao Zhao,Shu-yuan Liu,Han-ding Mao,Jun Ma,Ling Xing,Li-feng Wang,Wei-jia Zhi,Qing Song
Background: The effects of heat acclimation (HA) on the hypothalamus after exertional heatstroke (EHS) and the specific mechanism have not been fully elucidated, and this study aimed to address these questions. Methods: In the present study, rats were randomly assigned to the control, EHS, HA, or HA + EHS groups (n = 9). Hematoxylin and eosin (H&E) staining was used to examine pathology. Tandem mass tag (TMT)-based proteomic analysis was utilized to explore the impact of HA on the protein expression profile of the hypothalamus after EHS. Bioinformatics analysis was used to predict the functions of the differentially expressed proteins. The differential proteins were validated by western blotting. An enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines in the serum. Results: The H&E staining (n = 5) results revealed that there were less structural changes in hypothalamus in the HA + EHS group compared with the EHS group. Proteomic analysis (n = 4) revealed that proinflammatory proteins such as argininosuccinate synthetase (ASS1), high mobility group protein B2 (HMGB2) and vimentin were evidently downregulated in the HA + EHS group. The levels of interleukin (IL)-1β, IL-1, and IL-8 were decreased in the serum samples (n = 3) from HA + EHS rats. Conclusions: HA may alleviate hypothalamic damage caused by heat attack by inhibiting inflammatory activities, and ASS1, HMGB2 and vimentin could be candidate factors involved in the exact mechanism.
研究领域:
生理学
·
蛋白质组学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STAR Protocols
·
2024
·
IF 1.3
Siyao Zhou,Shumin Duan,Hongbin Yang
Summary To exclude the influence of motion on in vivo calcium imaging, animals usually need to be fixed. However, the whole-body restraint can cause stress in animals, affecting experimental results. In addition, some brain regions are prone to bleeding during surgery, which lowers the success rate of calcium imaging. Here, we present a protocol for calcium imaging using heparin-treated fiber in head-fixed mice. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, fiber photometry, and data analysis. For complete details on the use and execution of this protocol, please refer to Du et al. 1
研究领域:
神经科学
·
生物医学工程
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STAR Protocols
·
2026
·Vol. 7
·期 3
·104750
·
IF 1.4
Zhuoyun Tong,Yiming Zhu,Haimei Lv,Shuangshuang Liu,Chunliang Xu
Summary Plasma cells are essential for long-term humoral immunity and are enriched in the bone marrow. Here, we present a protocol for selective labeling of plasma cells within a single mouse femur, using an intrafemoral antibody injection approach. We describe steps for the direct injection of fluorochrome-conjugated antibodies into the medullary cavity. We then detail procedures for bone marrow cell collection and analysis by flow cytometry. This protocol enables investigation of bone marrow plasma cell retention, redistribution, and mobilization. For complete details on the use and execution of this protocol, please refer to Zhu et al. 1
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STAR Protocols
·
2025
·Vol. 7
·期 1
·104268
·
IF 1.3
Jiahui Song,Wubin Zhao,Junkai Zhang,Kewei Hu,Bohao Ma,Shilan Wang,Yichao Zhao,Jun Pu
Summary The parabiotic mouse model is widely utilized across various research fields due to its characteristic shared circulation system. Here, we present a protocol for constructing and validating parabiotic mouse models by flow cytometric analysis of immune cells. We describe steps for cohabitation, parabiosis surgery, sampling, and flow cytometric validation. This protocol improves reliability and allows significantly improved short- and long-term survival rates compared to existing approaches. For complete details on the use and execution of this protocol, please refer to Zhao et al. 1
研究领域:
免疫学
·
实验医学
·
转化研究
·
小鼠模型
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Chengji Dong,Gaoxiang Yu,Xianhui Ma,Zhuliu Chen,Ningning Yang,Xuankuai Chen,Yingying Lai,Jiayi Zhao,Yanlan Gu,Kailiang Zhou,Weiyang Gao,Jian Ding,Xuwei Zhu
Background How to improve flap necrosis in distal flaps is still a popular topic in clinical flap research. Zingerone (ZIN), as a plant extract, has therapeutic effects on many diseases. The purpose of our study was to explore whether ZIN can promote the survival of ischaemic skin flaps and its specific mechanism. Methods An ischemic flap model was established in the dorsal region of mice. The changes of angiogenesis, oxidative stress, apoptosis and Keap1-Nrf2 related proteins in the skin flap were detected by immunofluorescence, western blot and qPCR. Overexpression of Keap1 by adeno-associated virus (AAV) was used to study the specific pathway and mechanism. Results The results showed that ZIN can induce angiogenesis and reduce oxidative stress and apoptosis to promote flap survival. Increased expression of Keap1 reversed the therapeutic effect of ZIN. Conclusion ZIN activates Nrf2 by inhibiting Keap1 activation, thus promoting angiogenesis and inhibiting oxidative stress and apoptosis.
研究领域:
外科手术
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
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