INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xianglong Li,Haoxian Huang,Yachuan Yuan,Mingdong Wang,Xuanyu Cao,Yanbin Feng,Song Xue
Excessive CO 2 emissions have led to increasingly severe climate and environmental challenges. Within the framework of carbon neutrality, biological carbon fixation is a promising approach to improve the ecological environment and optimize energy systems. Salicylic acid decarboxylase (SAD) catalyzes the conversion of CO 2 and phenolic compounds into salicylic acid derivatives. However, the efficiency of CO 2 fixation in this process is low and is limited by the difficulty of product release during carboxylation. In this study, the engineered SAD, with the tunnel microenvironment remodeled to increase the turnover number ( k cat ), exhibited heightened CO 2 fixation efficiency. The mutant K23A/Y64T introduces a new tunnel architecture that indirectly enlarges the bottleneck radius of the native tunnel. These changes in the tunnel microenvironment facilitated product release, which was supported by evidence from both the catalytic activity and random acceleration molecular dynamics (RAMD). Kinetic analysis revealed that K23A/Y64T exhibited a 26-fold increase in catalytic efficiency ( k cat / K m ) with a K m consistent with that of the wild type, and the CO 2 conversion efficiency increased 14-fold. Dynamic cross-correlation matrices (DCCM) illustrated that α1 with K23A and loop5 with Y64T performed anticorrelated movements, enabling a change in the tunnel microenvironment between the two regions. Unlike conventional approaches targeting substrate–enzyme interactions, this study demonstrates that protein tunnel remodeling can intrinsically enhance carboxylation activity and holds potential for broad substrate applicability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Bingbing Liu,Lin Jiang,Lvyang Lin,Wulong Yang,Mian Li,Jianping Lin,Yiqi Jiang,Zehua Bao,Mianbin Wu
Xylitol is industrially synthesized by d-xylose, which is more expensive than glucose. However, de novo xylitol biosynthesis from glucose remains challenging in Escherichia coli. To address these challenges, this study first developed a biosynthetic pathway from glucose via an arabitol intermediate with arabitol phosphate dehydrogenase (APDH). Chassis optimization through knocking out key genes (rpiA/B, pgi, tktA, tktB, xylB) enhanced flux and increased arabitol yield to 1.61 g/L. Subsequently, the arabitol-to-xylitol route was established using arabitol dehydrogenase (ArDH2) and xylitol dehydrogenase (XDH1) enzymes, showing a 52.1% conversion rate in vitro. The heterologous AXA module (APDH, ArDH2, XDH1) was multicopy integrated into the optimized chassis W3P-5ΔB. The strain W3P4A produced 5.04 g/L arabitol and 1.89 g/L xylitol in shake-flasks. Fed-batch fermentation with a plasmid-carrying strain achieved 5.11 g/L xylitol and 18.5% (g/g) yield from glucose. This work establishes a new platform for xylitol bioproduction from glucose and provides a foundational framework for industrial-scale implementation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2026
·
IF 2.6
Tianyi Zhu,Jian Zhang,Danyi He,Yiwen Yang,Hailong Wang,Jianbo Wang,Longhua Guo,Lei Li,Jianrong Chen,Yanbo Zeng
Oxytetracycline (OTC) residues have seriously threatened human health, as they may trigger allergic responses, hepatotoxicity, and the development of bacterial resistance. Detecting OTC in samples is therefore important. This work developed a pyrene-based fluorescent molecularly imprinted polymer (Py-FMIP) to rapidly and selectively detect OTC. N-allyl-4-(pyren-1-yl) butanamide (Al-Py), a new alkenyl functional monomer, was synthesized using 1-pyrenebutyric acid as the raw material with simple synthesis process. Py-FMIP was then obtained with Al-Py as a functional monomer and OTC as a template molecule. The Al-Py and Py-FMIP absolute fluorescence quantum yields were 74.05% and 69.27%, separately, suggesting the high fluorescence performances of two materials. The fluorescence of Py-FMIP was strongly quenched by OTC through inner filter effect. The prepared Py-FMIP sensor responded to OTC within 1 min, its linear detection range was 0.5–150 μM, and its detection limit was 0.05 μM (S/N = 3). The prepared Py-FMIP sensor also exhibited notable selectivity toward OTC. The recoveries of the Py-FMIP fluorescent sensor were 96.37%–104.60% in milk and tap water, with relative standard deviations lower than 5.13%.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Guo Hui,Jing Ding,Jiaxin Li,Shenglan Zhao,Yiting Deng,Peilong Ma,Longteng Wang
Gentamicin, a widely used veterinary antibiotic in animal husbandry, poses significant food safety risks due to its persistence in livestock products, soil, and water systems. Chronic exposure to gentamicin residues is associated with severe health effects, including ototoxicity, nephrotoxicity, and neuromuscular toxicity. To address this challenge, we developed a fluorescent sensing strategy by synthesizing a core–shell nanocomposite, Fe₃O₄@SiO₂@N-GQDs@MIPs (termed the MIPs probe). This probe was constructed by integrating nitrogen-doped graphene quantum dots (N-GQDs) with magnetic Fe₃O₄ nanoparticles, and the overall detection platform is referred to as the MIP sensor. The sensor operates via static quenching, enabling specific and sensitive detection of gentamicin with a linear range of 3–900 nM and a detection limit of 0.274 nM. The sensor was successfully applied to detect gentamicin in milk samples, demonstrating excellent recovery rates (94.0–103.3%) and precision (RSD < 2.2%). This work presents a robust, efficient, and field-deployable platform for monitoring antibiotic residues in food products, offering significant improvements over conventional chromatographic and immunological methods.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 7.7
Huikai Lin,Caiyi Du,Ningyi Xu,Yiru Wang,Chunyan Sun
CRISPR/Cas12a can activate trans-cleavage activity upon single-stranded DNA binding, making it widely applicable in highly sensitive fluorescent biosensors. Sensors utilizing monochromatic fluorescence output modes are susceptible to interference from multiple factors, resulting in unstable detection methods. To address this, we designed a ratio-based fluorescence sensor for detecting tetracyclines (TCs) based on the synergistic signal amplification of nucleic acid aptamer (42TET) and zirconium-based metal-organic framework (PCN-224) with CRISPR/Cas12a. PCN-224 adsorbs single-stranded DNA, quenching its FAM-labeled fluorescence while simultaneously emitting its own fluorescent signal. This dual-fluorescence output reduces background interference and enhances detection accuracy. By designing an activator as a complementary sequence (cDNA) to the nucleic acid aptamer, the system binds to 42TET in the presence of tetracycline, releasing the cDNA to activate the CRISPR/Cas12a system. Through this system's signal amplification capability, highly sensitive detection of tetracycline is achieved, with a detection limit reaching 8.7 nM. It possesses the same detection capabilities as traditional HPLC detection methods. This method is simple, rapid, efficient, and highly quantitative, offering broad prospects for future applications.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Chenyan Lu,Dajie Zou,Ning Zhang,Huye Li,Yanyan Jia,Huan Wang
In this study, the hexane extract of Asters tataricus was used as the carbon source, and ethylenediamine and 4-bromophenylene diamine were selected as dopants to prepare nitrogen and bromine co-doped carbon dots (N,Br-CDs) by the hydrothermal synthesis method. The obtained fluorescent probe has a uniform particle size distribution, good water solubility and high stability, with a fluorescence quantum yield of 48.43%. Under 420 nm excitation conditions, N,Br-CDs exhibited the strongest fluorescence emission at 513 nm, indicating that the sensing system was in the “ON” state. Subsequently, TA was introduced as a quencher to induce static quenching of the N,Br-CDs system, significantly weakening the fluorescence intensity and switching the sensing system from “ON” to “OFF”. Finally, after EM was introduced into the N,Br-CDs/TA system, EM preferentially formed a complex with TA and weakened its quenching effect on the quantum dots, thereby achieving fluorescence recovery and returning the sensing system from the “OFF” state to the “ON” state. Based on the above mechanism, an “ON-OFF-ON” fluorescence sensing system with N,Br-CDs as the luminescent substrate was constructed for the sensitive detection of TA and EM. The results showed that the system exhibited good linear response to TA, with the linear ranges being 1.50–22.94 μM and 34.88–348.42 μM, respectively, and the limits of detection being 0.2168 μM and 2.1797 μM, respectively (LOD, S/N = 3). The linear range for EM was 1.93–377.08 μM, with a detection limit of 0.6693 μM. Furthermore, the sensor system was capable of quantitatively determining TA and EM in human serum, Huangshui River water, and milk matrices, demonstrating its potential for broad application in environmental monitoring and bioanalysis.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2026
·
IF 6.1
Tai Ye,Shuying Yue,Long Bai,Haohao Chen,Chenyang Li,Rehemutula Wubuli,Kehua Dang,Min Yuan,Hui Cao,Ziqing Ye,Fei Xu
Co-immobilization of horseradish peroxidase (HRP) with G-quadruplex/hemin complex often suffer from low efficiency, intricate procedures, and reduced catalytic performance. To address these challenges, we developed a biomineralization strategy that integrates HRP and G-quadruplex/hemin into the single nanosheet structure. By engineering a split aptamer sequence into a G-rich domain, the nanosheets synergistically combine enhanced catalytic activity with better target-specific molecular recognition. Compared with either free HRP or it biomineralized counterpart alone, the prepared nanosheets exhibited markedly improved peroxidase-like activity. In the presence of enrofloxacin, specific aptamer-target binding facilitated the formation of a ternary complex on the electrode surface, enabling the catalysis of H 2 O 2 -mediated oxidation of TMB. Under the optimized conditions, the electrochemical aptasensor displayed a wide linear detection range of 1 nM–10 μM with a low detection limit of 0.53 nM. Beyond high selectivity, the sensor demonstrated excellent practical applicability in spiked milk and egg white samples. The generality of the developed platform was further confirmed by its successful extension to other antibiotics detection through replacing the split-aptamer pairs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2026
·
IF 3.1
Zheng Xinxiang,Xu Hui,Zhang Runke,Zhao Tengzhi,Wang Zhaofeng,Zhao Xingfei,Wang Hua
In this study, a dual-ratiometric optical probe utilizing europium-doped carbon dots (Eu-CDs) was developed for simultaneous fluorescent and colorimetric detection of tetracycline (TC). The Eu-CDs complex was synthesized through a hydrothermal approach. Upon coordination with Eriochrome Black T (EBT), the Eu-CDs complex exhibits a distinct magenta coloration. Introduction of TC triggers a ligand displacement reaction, resulting in color transition from magenta to blue, resulting in ratiometric colorimetric visual detection. Meanwhile, the TC-bound EBT-CDs@Eu complex emits ratiometric fluorescence change. A ratiometric fluorescence detection method was established for TC based on the absorbance-energy transfer-emission (AETE) mechanism, culminating in the successful design of a dual-ratiometric optical probe. The colorimetric assay demonstrated a detection limit (LOD) of 20.5 nM for TC. Furthermore, the practical applicability of this sensor was validated through successful TC detection in aquaculture samples, highlighting its potential for real-world monitoring applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 11.3
Quanfu Wang,Xingshun Li,Yatong Wang,Yanhua Hou
The persistent accumulation of poly(butylene adipate-co-terephthalate) (PBAT) in the environment poses severe ecological threats. While enzymatic degradation offers promise, most reported enzymes require elevated reaction temperatures. Furthermore, free enzymes often suffer from limitations such as poor stability and high production costs. This study identified and characterized an innovative cold-adapted arylesterase (CsAE). CsAE exhibited optimum activity at 20℃ while retaining 48.77 % residual activity at 0℃, demonstrating characteristics of a cold-adapted enzyme. The engineered bacterium BL21/pET-INPN-CsAE, constructed via INPN surface display technology, exhibited normal growth while maintaining outer membrane integrity. Compared to the free CsAE, the whole-cell catalyst exhibited markedly enhanced thermal stability, reusability, and long-term operational stability. Notably, the observed features on the PBAT film—including the appearance of pores and cracks, a decrease in the water contact angle, cleavage of ester bonds, a reduction in the amorphous phase, and the emergence of the degradation monomer terephthalic acid—collectively indicate that both CsAE and the engineered strain facilitated the degradation of PBAT. Bioremediation experiments confirmed that the engineered strain mediated a 20.46 % degradation of PBAT over 21 days at 15℃, demonstrating its efficacy under low-temperature conditions. Collectively, this work establishes INPN-mediated surface display of the cold-adapted CsAE as a sustainable biocatalytic strategy for PBAT remediation in cold environments.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 1
·60
·
IF 5.3
Yang Yu Zhu,Li Gui Yong,Zhang Pu,Cui Linyun,Liu Huan Li,Chen Shi,Dai Xin
In this study, N-CDs were combined with Eu 3+ to construct a ratiometric fluorescence sensor (N-CDs@Eu 3+ ) for the determination of tetracycline (TC). The nitrogen-doped carbon dots (N-CDs) with good water solubility, excellent fluorescence performance, and high stability successfully prepared by using the leaves of Mahonia fortunei leaf as the carbon source and ethylenediamine as the nitrogen source through a one-step thermal solvent method. In the presence of TC, the fluorescence intensity of N-CDs at 510 nm was quenched due to fluorescence resonance energy transfer (FRET), while the fluorescence intensity of Eu 3+ at 616 nm was significantly enhanced through the antenna effect (AE). This ratiometric fluorescence sensor enables rapid, sensitive and highly selective detection of TC, with a detection limit as low as 7.5 nM. Moreover, a distinct color change from green to red can be observed under a 365 nm UV lamp. With the assistance of a smartphone sensing platform, visual semi-quantitative monitoring of TC was achieved. This method was successfully applied to the detection of TC in milk, pork, and honey, with recoveries ranging from 90.6% to 105.0%. This research demonstrates that this sensor holds potential application value in the field of real-time visualization detection and semi-quantitative determination of TC in biomedical and food safety sensing fields. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 9.8
Xinge Ju,Guantong Liu,Xin Sun,Haiyang Lv,Zhanye Yang,Shutong Zhang,Yuan Tian,Bing Zhao
This work developed a dual-mode sensor based on aptamer regulation of silver core-cuprous oxide shell (Ag@Cu 2 O) nanozyme activity for kanamycin detection. The synthesized Ag@Cu 2 O nanoparticles served as both a surface-enhanced Raman scattering activity (SERS) substrate and a peroxidase-like catalytic activity. The aptamer adsorbed on the Ag@Cu 2 O surface enhanced the nanozyme activity through electrostatic interactions with positively charged 3,3′,5,5′-tetramethylbenzidine (TMB). This promoted the oxidation of TMB to blue oxidized 3,3′,5,5′-tetramethylbenzidine (ox-TMB) by hydrogen peroxide (H 2 O 2 ). Upon kanamycin binding, the colorimetric absorbance and SERS intensity decreased. The SERS mode achieved a linear range of 0.01–1 μM and the limit of detection (LOD) of 1.78 nM. The colorimetric mode provided a linear range of 0.1–1 μM and a LOD of 47.7 nM. Good recoveries (92.48–102.57%) and low relative standard deviations (RSD < 5.0%) were achieved in spiked honey and mutton samples. This dual-mode design offered potential for food safety monitoring of kanamycin residues.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Liang Zhu,Lejun Liao,Yan Huang,Zhou Nie,Chunyang Lei
The highly sensitive analysis of small-molecule targets holds profound significance across diverse fields, ranging from clinical diagnosis and environmental monitoring to food safety. Herein, we developed a label-free electrochemical CRISPR platform gated by allosteric transcription factors (aTFs) for the ultrasensitive detection of various small molecules. In this system, the specific binding of target analytes to their cognate aTFs induces the release of programmable DNA adaptors, which subsequently trigger Cas12a to trans-cleave DNA probes anchored to the electrode surface. Consequently, the truncated DNA probes serve as initiators to form electroactive G-quadruplex/hemin complexes in situ via terminal deoxynucleotidyl transferase (TdT)-mediated elongation, generating a robust electrochemical response signal. Using TetR as a model aTF, this integrated electrochemical CRISPR biosensor achieved tetracycline detection with picomolar sensitivity. Furthermore, the versatility of this platform was demonstrated by extending its application to p-hydroxybenzoic acid and copper ions through the simple substitution of the aTF modules. The practical utility of the assay was further demonstrated by the robust detection of tetracycline in complex matrices such as milk. Ultimately, this study not only provides a novel strategy for constructing universal, label-free electrochemical CRISPR platforms but also paves the way for the sensitive detection of low-abundance non-nucleic acid targets.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）