CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2017
·Vol. 45
·期 6
·830-836
·
IF 0.795
Hong-Cai ZHAO,Pu ZHANG,She-Hong LI,Hong-Xia LUO
A large area graphene platform electrode (GPE) was fabricated by transferring chemical vapor deposition (CVD) grown graphene on Cu foils onto polyethylene terephthalate (PET) substrates with the aid of polymethyl methacrylate (PMMA). Cobalt hexacyanoferrate (CoHCF) was electrodeposited on GPE by cyclic voltammetry. The morphology and electrochemical properties of CoHCF/GPE were studied by scanning electron microscope (SEM), electrochemical impedance spectroscopy (EIS) and the electrochemical measurements. The CoHCF/GPE showed favorable sensing effect toward H 2 O 2 . A novel non-enzyme H 2 O 2 sensor was constructed. Under the optimal experimental conditions, the proposed sensor responded to the addition of H 2 O 2 very quickly (about 2 s) with a wide linear rang (0.005–1.2 mM) and a low detection limit (7.1 nM). This sensor was easy to be fabricated and showed excellent stability and anti-interference ability.
研究领域:
电化学
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生物传感器
·
纳米材料
·
分子诊断
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转化医学
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即时检测
✓ 已验证
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Xinlian Zhang,Xi Chen,Yuhan Yao,Xiaona Shang,Huijun Lu,Wang Zhao,Sixiu Liu,Jianmin Chen,Guodong Sui
Acute toxicity assay presents vital significance in modern environmental monitoring, including online detection and in-situ assay for emergency events. Although photobacteria related detection methods were established and verified in the past decades with combination of photomultiplier tube (PMT), the price and size of PMT sensor hampered application of rapid acute toxicity assay and detection system miniaturization, especially in the resource-limited occasions. Wide application of smartphones with great low-light performance cameras could be used in photobacteria-based toxicity assay instead of the PMT methods. Herein a box-type portable detection system had been successfully established, including a disc-chip for detection, detection device, and smartphones with a high-performance camera. The system performed well showing stable temperature and rotation control. Results captured by CMOS-based camera presented a linear relationship with PMT-based detection method. An image progress algorithm was also established and tested by series diluted zinc sulfate solution as a reference substance. The system also performed well for toxicity analysis for real Atmospheric particle matter sample. The system could be used in some environmental monitoring scenarios as an alternative solution.
研究领域:
环境科学
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毒理学
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生物传感器
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微流控技术
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即时检测
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Yingyi Yao,Weisheng Guo,Jian Zhang,Yudong Wu,Weihua Fu,Tingting Liu,Xiaoli Wu,Hanjie Wang,Xiaoqun Gong,Xing-jie Liang,Jin Chang
Ultrasensitive and quantitative fast screening of cancer biomarkers by immunochromatography test strip (ICTS) is still challenging in clinic. The gold nanoparticles (NPs) based ICTS with colorimetric readout enables a quick spectrum screening but suffers from nonquantitative performance; although ICTS with fluorescence readout (FICTS) allows quantitative detection, its sensitivity still deserves more efforts and attentions. In this work, by taking advantages of colorimetric ICTS and FICTS, we described a reverse fluorescence enhancement ICTS (rFICTS) with bimodal signal readout for ultrasensitive and quantitative fast screening of carcinoembryonic antigen (CEA). In the presence of target, gold NPs aggregation in T line induced colorimetric readout, allowing on-the-spot spectrum screening in 10 min by naked eye. Meanwhile, the reverse fluorescence enhancement signal enabled more accurately quantitative detection with better sensitivity (5.89 pg/mL for CEA), which is more than 2 orders of magnitude lower than that of the conventional FICTS. The accuracy and stability of the rFICTS were investigated with more than 100 clinical serum samples for large-scale screening. Furthermore, this rFICTS also realized postoperative monitoring by detecting CEA in a patient with colon cancer and comparing with CT imaging diagnosis. These results indicated this rFICTS is particularly suitable for point-of-care (POC) diagnostics in both resource-rich and resource-limited settings.
研究领域:
生物医学工程
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临床诊断
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纳米生物技术
·
肿瘤学
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即时检测
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Haiming Qiu,Yong Yao,Yongkang Dong,Jiajun Tian
Immunosensors capable of ultralow-concentration and single-molecule detection of biomarkers are garnering attention for the early diagnosis of cancer. Herein, a fiber-optic Fabry–Perot interferometer (FPI)–based immunosensor was used for the first time for single-molecule detection of progastrin-releasing peptide (ProGRP). The cascaded FPI structure of the immunosensor introduces a high-order harmonic Vernier effect (HVE). A piece of a side-polished D-shaped hollow-core photonic crystal fiber (HCPCF) was used as a sensing FPI, on which the biomarker was deposited to detect ProGRP. Compared with traditional FPIs with open-cavity structures, this structure provided a larger contact area and improved the sensitivity of the immunosensor. The polished side surface of the D-shaped HCPCF was modified using a gold nanoparticle–graphene oxide (AuNP@GO) nanointerface to enhance refractive index (RI) modulation via antigen–antibody binding and achieve selective energy enhancement of the binding site . The antigen binding changes the RI of the D-shaped HCPCF and the effective RI of the transmitted light in the sensing FPI, thereby changing the spectrum of the immunosensor. Experimental results showed that the high-order HVE and AuNP@GO nanointerface considerably improved the immunosensor sensitivity, exhibiting a liquid RI sensitivity of 583,000 nm/RIU. After functionalization with an anti-ProGRP antibody, the limit of detection of the immunosensor for ProGRP reached 17.1 ag/mL; moreover, the immunosensor could perform detection at the single-molecule level. The proposed novel immunosensor overcomes the sensitivity limitations of optical devices and achieves single-molecule detection of a protein.
研究领域:
光学传感器
·
生物医学工程
·
癌症诊断
·
纳米生物技术
·
即时检测
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）