Drug Design Development and Therapy
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2022
·
IF 4.319
Yi Zhang,Xiujin Shi,Jialun Han,Wenxing Peng,Zhenwei Fang,Yang Zhou,Xiaoyu Xu,Jie Lin,Fucheng Xiao,Limin Zhao,Yang Lin
Introduction As the primary immune cells, macrophages play a key role in atherosclerotic progression. M2 macrophage polarization has been reported to promote tissue repair and attenuate plaque formation upon the expression of anti-inflammatory factors. Convallatoxin (CNT) is a natural cardiac glycoside with anti-inflammatory pharmacological properties. However, whether CNT protects against atherosclerosis (AS) and underlying mechanisms is unknown. This work was designed to explore the potential effects of CNT on atherosclerosis.Methods In this study, Apolipoprotein E deficiency (ApoE−/-) mice fed with high-fat diet were established, and CNT (50 or 100 μg/kg) were intragastrically administrated for 12 weeks every day. In vitro, RAW264.7 macrophages stimulated with ox-LDL were treated with CNT (50 or 100 nM) for 24 h. The specific PPARγ antagonist, GW9662, was used to block the PPARγ signaling pathway in vitro. Then, the atherosclerotic lesions, macrophage polarization markers, inflammatory cytokines and PPARγ signaling pathway were examined in further examinations.Results Our results showed that the atherosclerotic lesions were reduced by CNT, as demonstrated by the downregulation of serum lipid level and aortic plaque area in AS mice. Furthermore, we found that CNT treatment promoted the expression of M2 macrophage markers (Arg1, Mrc1, Retnla and Chi3l3), and decreased the levels of pro-inflammatory cytokines (IL-6 and TNF-α), accompanied by the increase of anti-inflammatory factor (IL-10) in aortic vessels of AS mice. In ox-LDL-induced RAW264.7 cells, CNT administration also facilitated macrophages polarizing towards M2 subtype and inhibited inflammatory responses. Furthermore, both the in vivo and in vitro experiments showed CNT could increase the expression of PPARγ, Integrin αv and Integrin β5, and GW9662 could block CNT-induced M2 macrophage polarization.Conclusion Taken together, these data suggest that CNT may promote M2 macrophage polarization to exert an anti-atherosclerotic effect, partially through activating PPARγ-Integrin αvβ5 signaling pathway.
研究领域:
心血管疾病
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免疫学
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药理学
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细胞生物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2025
·
IF 1.9
Menglu Li,Ruotong He,Ceyao Yang,Hao Chen,Chaoling Wu,Xiaomiao Zhang,Xin Fu,Chengbo Lu,Liu Yang,Shi Liu,Yi Zhang
Lilium brownii F.E.Brown var. viridulum Baker (Longya Baihe), a traditional medicinal and edible plant. In order to study the activity of its polysaccharide extracts, a three-factor three-level Box-Behnken response surface design was employed to optimize the extraction process. The optimal extraction conditions include: liquid-solid ratio of 32:1 mL/g, extraction temperature of 70 °C, and extraction time of 3.5 h. The purified Longya Baihe polysaccharide (LP), obtained via DEAE-52 cellulose chromatography, was structurally characterized by molecular weight distribution, monosaccharide composition and infrared spectrum. The results show that LP was a macromolecular polysaccharide composed of glucose and mannose (molar ratio of 1:1.56), with an average molecular mass (Mn) of 24.006 kDa. In vitro assays demonstrated its excellent antioxidant and anti-inflammatory activities. LP significantly inhibited lipopolysaccharide (LPS)-induced activation of the nuclear factor kappa-B (NF-κB) pathway in RAW264.7 cells, downregulated the overexpression of nitric oxide (NO), interleukin (IL)-6, inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α, and enhancing the levels of the anti-inflammatory factor interleukin (IL)-10. This study suggests LP holds promise as a natural antioxidant and anti-inflammatory agent for potential development and application.
研究领域:
植物化学
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天然产物化学
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生物活性多糖
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食品科学与技术
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生药学
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炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Agronomy-Basel
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2025
·Vol. 15
·期 9
·2211
·
IF 3.4
Zheng Zhang,Jingfang Wei,Leifeng Xu,Jun Ming,Panpan Yang
Lilium browniivar.viridulumis a popular natural health product that combines medicine and food. However, the lack of high-quality and efficient propagation technology has been a significant impediment to the development of theL. browniivar.viridulumindustry. In this study, bulb scales ofL. browniivar.viridulumwere used as materials to investigate the induction of bulblets from scales, as well as the proliferation and enlargement of the induced bulblets. An in vitro regeneration system forL. browniivar.viridulumwas successfully established. The most effective methods for scale disinfection were found to be 75% alcohol disinfection for 30 s and 10% Sodium Hypochlorite (NaClO) disinfection for 15 min. The recommended medium for scale differentiation was Murashige and Skoog (MS) + 0.05 mg·L−11-Naphthaleneacetic acid (NAA) + 0.25 mg·L−1Thidiazuron (TDZ) + 0.05 mg·L−1kinetin (KIN) + 30 g·L−1sucrose + 6 g·L−1Activated Charcoal (AC). The recommended medium for bulblet proliferation was MS + 1.5 mg·L−16-benzylaminopurine (6-BA) + 0.5 mg·L−1NAA + 30 g·L−1sucrose. The recommended medium for bulblet enlargement was Modified MS Medium (potassium dihydrogen phosphate (KH2PO4, KDP) doubled) + 90 g·L−1sucrose. Bulblets with diameters of 1.5 cm were transplanted, and the germination rate was 100%. This study established a systematic in vitro regeneration system forL. browniivar.viridulum, which will provide the foundation for industrial production ofL. browniivar.viridulumbulbs and improvement through genetic transformation.
研究领域:
植物学
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植物组织培养
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园艺学
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植物繁殖
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）