Plants-Basel
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2021
·Vol. 10
·期 6
·1112
·
IF 3.935
Yan Yang,Liangfang Dai,Decai Wu,Limin Dong,Yisheng Tu,Jiankun Xie,Xiangdong Luo
Huperzia serratais a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation ofH. serratais considered a new way to relieve the resource pressure ofH. serrata. In this study, three different genotypic wildH. serratawere used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regeneratedH. serratawere investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium forH. serratawas the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1Naphthalene acetic acid (NAA) and 0.1 mg·L−12,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitroH. serratahad higher antioxidant activity than that of wildH. serrata. This study provides a reliable in vitroH. serrataculture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.Keywords:genotypes;Huperzia serrata;Huperzine A;micropropagation;sterilization;antioxidant
研究领域:
植物学
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药用植物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Guifang Qin,Jie Lei,Sihui Li,Yongmei Jiang,Lei Qiao,Mengdie Ren,Qiong Gao,Changwei Song,Shaobin Fu,Jinquan Zhou,Shiji Xiao,Maosheng Zhang,Gang Wang
In this study, ultrasonic-assisted deep eutectic solvents (DESs-UAE) was applied for efficient and environmentally friendly extraction of amentoflavone (AME) and robustaflavone (ROB) from Selaginella uncinata. Firstly, proline-lactic acid (Pro-Lac) was screened as the optimal DES from the 30 DESs. The maximum extraction rates of AME and ROB were obtained adopting the response surface methodology (RSM). Under the optimal conditions of molar ratio of 1:4, water content of 33 %, and extraction time of 30 min, the extraction yields of AME and ROB were 709.72 ± 11.31 μg/g and 145.13 ± 9.77 μg/g, respectively. Compared with the traditional extraction methods, the DESs-UAE extraction could enhance the extraction rates and save extraction time. At the same time, 23 flavonoids were identified by UPLC-Q-TOF-MS, of which 6 components appeared for the first time in S. uncinata. In addition, three antioxidant experiments (DPPH method, ABTS method, and hydroxyl radical scavenging method) were carried out, and the results showed that the S. uncinata extracts had potential antioxidant activity. Furthermore, S. uncinata extracts exhibited obvious growth inhibitory effect on A549, HCT-116, HT-29, sw1990, HepG2 and HeLa cancer cells. In conclusion, deep eutectic solvent, as an promising solvent, is much more effective and simple extraction of bioactive constituents from S. uncinata with high safety and low toxicity.
研究领域:
药物化学
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分析化学
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植物科学
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生物技术
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肿瘤学
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Ling Zhang,Qi-Bin Yang,Jia-Dong Shao,Can-Qi Liu,Lei Wu,Kong-Kai Zhu,Lin-Fu Liang
Chemical investigation on Palhinhaea cernua by HSQC-based small molecule accurate recognition technology (SMART) resulted in the rapid discovery of four triterpenes, including a new triterpene ( 1 ) with a rare picane-type carbon framework. The chemical structure of 1 was elucidated by analysis of spectral data. Moreover, the absolute configuration of 1 was established by TDDFT-ECD and QM-NMR calculations. Compounds 1 and 2 displayed significant tyrosinase inhibitory effects (IC 50 = 123.6 and 184.6 μM), which were similar to the positive control ascorbic acid (IC 50 = 128.1 μM). Docking study disclosed the multiple stabilizing interactions between 1 and oxy-tyrosinase. Moreover, molecular dynamics (MD) simulations and binding free energy analyses further validated the stable accommodation of compound 1 within the protein binding pocket.
研究领域:
天然产物化学
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药物化学
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分子生物学
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计算化学
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酶抑制剂研究
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
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IF 10.7
Pei Lin,Qiqi Wang,Qi Wang,Jiayun Chen,Liangliang He,Zifei Qin,Shaoping Li,Jingyan Han,Xinsheng Yao,Yang Yu,Zhihong Yao
Allium Macrostemon Bge. (AMB) is a well-known homology of herbal medicine and food that has been extensively used for thousands of years to alleviate cardiovascular diseases. It contains a significant amount of polysaccharides, yet limited research exists on whether these polysaccharides are responsible for its cardiovascular protective effects. In this study, the anti-atherosclerosis effect of the crude polysaccharides of AMB (AMBP) was evaluated using ApoE −/− mice fed a high-fat diet, along with ox-LDL-induced Thp-1 foam cells. Subsequently, guided by the inhibitory activity of foam cells formation, a major homogeneous polysaccharide named AMBP80-1a was isolated and purified, yielding 11.1 % from AMB. The molecular weight of AMBP80-1a was determined to be 10.01 kDa. AMBP80-1a was firstly characterized as an agavin-type fructan with main chains consisting of →1)- β - d -Fru f -(2→ and →1,6)- β - d -Fru f -(2→ linked to an internal glucose moiety, with →6)- β - d -Fru f -(2→ and β - d -Fru f -(2→ serving as side chains. Furthermore, the bio-activity results indicated that AMBP80-1a reduced lipid accumulation and cholesterol contents in ox-LDL-induced Thp-1 foam cell. These findings supported the role of AMBP in alleviating atherosclerosis in vivo/vitro . AMBP80-1a, as the predominant homogeneous polysaccharide in AMB, was expected to be developed as a functional agent to prevent atherosclerosis.
研究领域:
药理学
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心血管疾病
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分子生物学
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营养保健品
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DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2023
·Vol. 37
·期 5
·e5611
·
IF 1.8
Zhiyu Fan,Gangqiang Yang,Xinjie Wu,Yanan Yang,Jinfang Xu
Biflavonoids are naturally occurring compounds consisting of two flavonoid moieties that have received substantial attention from researchers. Although many kinds of biflavonoids are typically distributed in Selaginella uncinata with hypoglycemic effect, their anti- α -glucosidase activities are not yet clear. In this study, a ligand fishing strategy for fast screening of α -glucosidase inhibitors from S. uncinata was proposed. α -Glucosidase was first immobilized on Fe 3 O 4 magnetic nanoparticles (MNPs) and then the α -glucosidase–functionalized MNPs were incubated with crude extracts of S. uncinata to fish out the ligands. Furthermore, considering the similarity and easy confusion of the structures of biflavonoids, the fragmentation patterns of different types of biflavonoids were studied. Based on this, 11 biflavonoids ligands with α -glucosidase inhibitory activities were accurately and quickly identified from S. uncinata with ultra-high-performance liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry. Furthermore, these ligands were confirmed to be potential inhibitors through the in vitro inhibitory assay and molecular docking.
研究领域:
药理学
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分析化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2022
·Vol. 395
·期 10
·1283-1295
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IF 3.195
Xiong Ziwei,Cui Yushun,Wu Jiahui,Shi Lingyu,Quan Wen,Yang Shilin,Feng Yulin
Pteris cretica L. var. nervosa is one of the most well-known Chinese medicines. Although it is widely used to treat jaundice hepatitis, the main ingredient for its treatment was not thoroughly explored until recently. Essentially, the purpose of this study is to find the monomer compound in Pteris cretica L. var. nervosa , which is most likely to be effective in treating liver injury. Through the model of LPS/D-gal-induced liver injury in mice, the best therapeutic site of the total extract was explored, the chemical components of the parts with the best therapeutic effect were separated, a total of 10 flavonoids were isolated, and the RAW264.7 cells induced by LPS were used as the experimental model to explore the preliminary anti-inflammatory activity of NO production in vitro. Finally, the anti-inflammatory activity and the highest content in this plant Luteolin-7-O-rutinoside (LUT) were selected, as the object of study in vivo. It was found that LUT could not only reduce alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, but also significantly reduce the release of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β), and inhibit PI3K/AKT/AMPK/NF-κB pathway. In addition, LUT can increase levels of SOD and GSH to reduce oxidative stress. It has an obvious therapeutic effect on acute liver injury induced by LPS/D-gal in mice. Therefore, infer LUT is a functional substance in Pteris cretica L. var. nervosa. Graphical abstract
研究领域:
药理学
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免疫学
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肝脏病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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