ACS Nano
·
2018
·
IF 13.709
Wenfei Chen,Ying Wang,Ming Qin,Xudong Zhang,Zhirong Zhang,Xun Sun,Zhen Gu
The facultative anaerobe Salmonella strain VNP20009 selectively colonizes into tumors following systemic injection due to its preference for the hypoxia in the tumor cores. However, the phase 1 clinical trial of VNP20009 has been terminated mainly due to its weak antitumor effects and exhibition of dose-dependent toxicity. Here, we leveraged the advantages of VNP20009 biotherapy together with polydopamine-mediated photothermal therapy in order to enhance the antitumor efficacy toward malignant melanoma. VNP20009 was coated with polydopamine via oxidation and self-polymerization, which was then injected into tumor-bearing mice via the tail vein. Polydopamine-coated VNP20009 targeted hypoxic areas of the solid tumors, and near-infrared laser irradiation of the tumors induced heating due to polydopamine. This combined approach eliminated the tumors without relapse or metastasis with only one injection and laser irradiation. More importantly, we found both VNP and pDA potentiate the therapeutic ability of each other, resulting in a superior anticancer effect.
研究领域:
肿瘤学
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生物医学工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Sha Ni,Changsheng Qu,Xinhong Gan,Liang Ding,Weiyi Xia,Ying Teng,Yingge Shu,Wenjie Ren
Plant-microorganism combined bioremediation is a highly efficient and environmentally sustainable method for the remediation of contaminated soils. Despite its potential, the synergistic effects of wetland plants and anaerobic microbial consortium on the removal of chlorinated hydrocarbons (CAHs) from soil remain inadequately understood. In this study, an anaerobic bacterial consortium, capable of completely dechlorinating trichloroethylene (TCE), was enriched and screened from long-term CAH-contaminated soil. Subsequently, the combined effects of the wetland plant Typha angustifolia L. and the anaerobic bacterial consortium on the removal of TCE from soil were investigated, along with the underlying microbial mechanisms. The results demonstrated that the anaerobic bacterial consortium was able to completely convert 0.5 mM TCE to vinyl chloride (VC) within 7 days, and subsequently degrade VC to ethylene within 48 days. The integration of Typha angustifolia L. with the anaerobic bacterial consortium significantly enhanced both the removal and complete dechlorination of TCE from the soil compared to either treatment alone. Furthermore, this combination substantially increased the diversity and richness of soil bacterial communities, enriched dechlorinating microorganisms, and elevated the relative abundance of dehalogenating enzymes and peroxidases involved in pollutant degradation. In addition, the combination resulted in a more complex soil bacterial community, closer microbial interactions, and a more stable microbial co-occurrence network. This study extends the scope of plant-microorganism combined bioremediation for CAH- contaminated soils.
研究领域:
环境科学
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微生物学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Ming Li,Kang Lv,Shiqiang Wu,Shuiliang Chen
The anodophilic bacteria in the anodes of microbial fuel cells (MFCs) used to catalyze carbon oxidation are anaerobes and require anaerobic conditions; their bioelectrocatalytic activity will be greatly suppressed upon direct exposure to O2. In this study, an aerotolerant bioanode was fabricated for the first time by immobilization of anodophilic bacteria for use in MFCs operating under aerobic conditions. The fabrication of the aerotolerant bioanode was realized through the electrochemically induced penetration and propagation of anodophilic bacteria in a three-dimensional hydrogel scaffold. Under the protection of the hydrogel scaffold, the anodophilic bacteria exhibited excellent bioelectrocatalytic activity under continuous O2 aeration and delivered a current density comparable to that under anaerobic conditions. The MFC equipped with the aerotolerant bioanode has the potential to be applied to traditionally aerobic wastewater treatment (WWT) technology. This study offers new insight into the application of MFCs for WWT.
研究领域:
环境科学
·
微生物学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2025
·
IF 5.4
Min Ru,Renyan Huang,Lu Wang,Zuwei Luo,Ying Huang,Renchuan You,Ruoxuan Peng,Shuqin Yan,Qiang Zhang,Shengjie Ling,Weilin Xu
In response to the difficulties posed by anaerobic bacterial infections in wound healing, particularly in light of the increasing prevalence of antibiotic-resistant strains and the complex nature of wound environments, this study introduces a meticulously planned oxygen-generating tissue engineering scaffold to address these obstacles. This scaffold is realized from silk fibroin (SF), poly(vinyl alcohol) (PVA), and calcium peroxide (CPO) by ice crystal-induced self-assembly combined with the ice template method, aiming at a synergistic balance of hydrophilicity, structural stability, mechanocompatibility with biological tissues, controllable oxygen-releasing behavior, and antianaerobic bacterial properties. In particular, the oxygen-generating scaffold containing 2.5% CPO exhibits excellent antianaerobic properties by destroying C. perfringens through a mechanism that disrupts bacterial cell membranes and DNA, as well as promotes the generation of reactive oxygen species. Meanwhile, significant cytotoxic or hemolytic effects were avoided. Subsequent results demonstrated that the oxygen-generating scaffold accelerated the healing process of C. perfringens-infected wounds with results similar to those of amoxicillin. These results emphasize the promise of this new platform in clinical practice for the treatment of anaerobic bacterial infections and establishing a basis for advanced therapeutic strategies.
研究领域:
生物材料
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组织工程
·
感染生物学
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伤口修复
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抗菌治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Xuran Liu,Xianli Yang,Jingnan Yang,Yanxin Wu,Dongbo Wang
The quality and quantity of short chain fatty acids (SCFAs) production from anaerobic fermentation of sewage sludge is generally constrained by low organics solubilization and poor substrate-availability. This study provided an environmentally friendly strategy with zero valent iron (ZVI) activating sulfite pretreatment to overcome this limitation and advance the sludge anaerobic fermentation. Experimental results showed that the maximal SCFAs production and acetate proportion promoted by 5.7 and 2.75 times respectively at 500 mg S/L and the ZVI/sulfite ratio of 1.25. Mechanism analysis elucidated that the free radicals of S O 4 - · and ·OH produced from ZVI-sulfite pretreatment accelerated the disintegration of sludge-flocs and decreased the value of α-helix and ratio of α-helix/ (β-sheet + random coil) from 21.64 % and 44.23 % to 17.80 % and 38.67 %, respectively, which provided more easily- and bio- available substance for functional anaerobes. The ZVI-sulfite pretreatment enhanced the ability of functional anaerobes for SCFAs production processes as hydrolysis, acidogenesis and acetogenesis and reduced the SCFAs consumption as methanogenesis, which ultimately promoted SCFAs accumulation. Microbial community illustrated that ZVI-sulfite pretreatment enriched the relative abundance of functional anaerobes related to hydrolysis, acidogenesis and acetogenesis bioprocesses. PICRUSt analysis demonstrated that the pretreatment boosted the functional genes abundance involved in pathway of butyrate and acetate formation. Moreover, the enrichment genes of acetate formation were consistent with the increase of acetate proportion.
研究领域:
环境工程
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微生物学
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生物技术
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污水处理
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2023
·
IF 4.6
Mohamed S. Selim,Nesreen A. Fatthallah,Shimaa A. Higazy,Xiang Chen,Zhifeng Hao
For the first time, we developed a novel nanocomposite of RGO decorated with α-Mn 2 O 3 nanorods (NRs) for inhibiting microbially influenced corrosion . RGO sheets with 2 nm thick were effectively synthesized by the modified Hummers technique and then a hydrothermal reduction procedure. The fabricated RGO/α-Mn 2 O 3 nanocomposite exhibited controlled morphology, geometry, surface exposure, and orientation. Single crystal α-Mn 2 O 3 NRs with mean diameter of 50 nm and length of 1 μm were produced via the hydrothermal procedure to yield γ-manganite which was calcined at 700 °C. Stable RGO/α-Mn 2 O 3 nanocomposite was successfully prepared via an efficient two-phase ultrasonication approach. The structures of the developed materials and nanocomposite were detected via different characterization techniques. Cost-effectiveness, long-term antimicrobial activity, and biocorrosion inhibition are the major advantages of the produced RGO/α-Mn 2 O 3 nanocomposite. The nanocomposite's biocorrosion-protection was influenced by its high surface area and controlled size and morphology. Its antimicrobial mechanism against different anaerobic and aerobic microbial strains was deeply highlighted. Field emission SEM images showed that the blade-like RGO/α-Mn 2 O 3 nanocomposite cruelly attached into the microbial cell walls forming morphology disruption, cell wrapping, and microbial killing. This nanocomposite exhibited ultrahigh potency against the anaerobic sulfate-reducing bacteria (SRB) strain Desulfovibrio halophilus SL 890 at 150 mg/L. Its highest potency against most of the tested aerobic strains was achieved at 2 μg/mL for Kocuria rhizophila EMCC 1246, Bacillus subtilis EMCCN 1152, Pseudomonas aeruginosa NRRL B-800, Staphylococcus aureus ATCC 25923, Aspergillus brasiliensis ATCC 9642 , and Candida albicans ATCC 24433. The designed RGO/α-Mn 2 O 3 nanocomposite provide a new platform for microbial growth inhibition.
研究领域:
材料科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2022
·
IF 7.033
Xin Zhang,Yang Zhang,Ning Wang,Yetong Shen,Qing Chen,Lu Han,Bo Hu
NCs (1:1:4, 6 μL) to synergistically reduce the viability of HCT116 cells to 15.63± 2.90%.Conclusion: By using NIR-irradiation, the “transitive” nanoheaters can remotely control the activity of both bacteria (germinated from spore) and cancer cells. This discovery provides basis and a feasible plan for controllable safer treatment of bacteria therapy, especially anaerobes with spores in hypoxic areas of the malignant solid tumors.
研究领域:
癌症研究
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生物医学工程
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纳米技术
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治疗递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shahab Uddin,Sun Shao Yan,Wei-Zhong Zhang,Qian-Wen Zhao,Jia-Xu Chen,Zhaorong Yue,Yang Li,Xin Wang,Hongyu Li
Periodontitis, a progressive inflammatory condition, causes periodontal tissue destruction, pain, chewing difficulties, and tooth loss, primarily due to pathogenic oral biofilms dominated by low abundance periodontopathic bacteria. Accurate diagnosis is hindered by the limited bacterial presence in saliva, necessitating effective enrichment techniques. This study introduces novel amoxicillin-loaded chitosan-coated magnetic nanocarriers (AMX-CS-MNCs) designed for rapid capture, enrichment, and DNA extraction of Gram-positive and Gram-negative anaerobic periodontal pathogens, including Porphyromonas gingivalis and Parvimonas micra , from artificial and clinical saliva samples collected at Kangmei Dental Hospital, facilitating highly sensitive PCR-based detection. The chitosan coating enhances biocompatibility and dispersibility, while amoxicillin acts as a broad-spectrum targeting ligand. Comprehensive characterization using X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), zeta-potential analysis, dynamic light scattering (DLS), vibrating sample magnetometry (VSM), and energy-dispersive X-ray spectroscopy (EDS) confirms successful functionalization and drug loading. AMX-CS-MNCs demonstrate exceptional bacterial capture efficiency (>90 %) at low concentrations following preconcentration, integrating with PCR to achieve a limit of detection (LOD) of 10 CFU/mL—a 1000-fold improvement over conventional PCR. Validation with patient samples demonstrated robust DNA amplification, highlighting this platform's potential for early, sensitive, and selective periodontitis clinical diagnosis and targeted therapy.
研究领域:
纳米技术
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生物医学工程
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微生物学
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牙科医学
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分子诊断学
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药物递送
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 25
·2214105
·
IF 19
Peipei Zhu,Cheng Zhou,Jiafei Chen,Qiang Chu,Feiyu Li,Yike Fu,Xiang Li
The combination of precise tumor depletion and immunotherapy presents great potential to exterminate cancer disease. Anaerobic bacteria can trigger innate and adaptive immune responses in tumors to facilitate the antitumor effects. However, bacteria injected intravenously induce systemic inflammation and are likely to be eliminated in the blood. Herein, live bacteria, Propionibacterium acnes ( P. acnes ), are integrated with ZnAl layered double hydroxides (LDHs) to construct an effective system enabling synergistic inhibition in both tumor growth and metastasis. In this system, P. acnes carries LDHs to reach and penetrate the hypoxic tumor tissue. The metabolism of colonized P. acnes and the pH-responsive degradation of LDHs produce reactive nitrogen species and reactive oxygen species to trigger inevitable cell death. Furthermore, the decomposition of LDHs exposes P. acnes , and thus the immune response is activated to prohibit the reoccurrence of distant tumors. The biohybrid P. acnes @LDHs, developed in this study, possesses remarkable anticancer outcomes both in vitro and in vivo.
研究领域:
癌症生物学
·
免疫学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Yanzhuo Zhang,Rui He,Jing Zhao,Xiaozhuan Zhang,Alexandr V. Bildyukevich
For the first time, biochar was prepared by changing the polystyrene (PS) content in sludge, and the efficiency of antibiotics removal by biochar was evaluated after fermentation aging. Fermentation aging affects the efficiency of antibiotics removal by reducing the specific surface area and active sites of biochar. The antibiotics removal efficiency of different types of biochar after aging decreased by 5.95%–13.59%. Owing to the biotoxicity of biochar, the relative abundance of most communities decreased during fermentation, whereas Anaerolineae still increased (14.29% to 33.05% or 33.02%). However, controlled experiments confirmed that biochar was much less toxic to Scenedesmus obliquus than to antibiotics, with concentrations of 11.09 × 10 5 cells/mL and 0.188 × 10 5 cells/mL, respectively. With the positive effect of environmentally persistent free radicals (EPFRs) considered, increasing the PS content in sludge facilitated the removal of antibiotics by biochar. This study assesses the stability of biochar in removing antibiotics after long-term microbial aging.
研究领域:
环境科学
·
生物技术
·
微生物学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Min He,Jiajia Xue,Huan Geng,Hao Gu,Dafu Chen,Rui Shi,Liqun Zhang
Here, with the aim of inhibiting inflammation during guided tissue regeneration membrane (GTRM) implant surgery, coaxial electrospinning was used to fabricate drug-loaded core/sheath nanofiber GTRMs capable of controlled drug release. Various amounts of the anti-inflammatory agent metronidazole (MNA) were encapsulated into the core/sheath nanofibers (where PCL was the core, gelatin the sheath, and the gelatin shell was crosslinked with genipin) in order to establish the minimal drug content necessary to achieve the appropriate anti-inflammatory effect. By using TEM and SEM, the core/sheath structure was confirmed. In vitro drug disolution results showed that the core/sheath nanofibers exhibited sustained release profiles that were superior to those nanofibers produced by blending electrospinning. Additionally, the membrane significantly inhibited the colonization of anaerobic bacteria. Furthermore, with gelatin as a shell, the core/shell nanofiber membranes showed improved hydrophilicity, which resulted in better cell adhesion and proliferation without cytotoxicity. Therefore, in this study, a simple and effective coaxial electrospinning approach was demonstrated for the fabrication of anti-inflammatory GTRMs capable of providing controlled drug release.
研究领域:
材料科学
·
生物医学工程
·
药剂学
·
组织工程
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Yuanxin Zeng,Wenyi Dong,Hongjie Wang,Xiao Huang,Ji Li
To obtain high-quality VFAs production from primary sludge , a novel strategy that combined peroxymonosulfate (PMS) pretreatment and alkaline fermentation (i.e., PMS & pH9) was proposed in the study. The results showed that PMS & pH9 was efficient in sludge solubilization and hydrolysis, resulting in a maximal VFAs yield of 401.2 mg COD/g VSS, which was 7.3-, 2.1-, and 8.8-fold higher than the sole PMS, sole pH9, and control, respectively. Acetate comprised 87.6% of VFAs in this integration system. Mechanism investigations revealed that sulfate and free radicals produced by PMS play roles in improving VFAs yield under alkaline conditions. Besides, sulfate also aided in C3∼C5 VFAs converting to acetate under alkaline conditions depending on the increase of incomplete-oxidative sulfate-reducing bacteria (iso-SRB) (i.e., Desulfobulbus and Desulfobotulus ). Moreover, the relative abundances of acid-forming characteristic genera (i.e., Proteiniborus , Proteinilcasticum , and Acetoanaerobium ) were higher in PMS & pH9.
研究领域:
环境工程
·
生物技术
·
废水处理
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）