Frontiers in Chemistry
·
2021
·
IF 5.221
Chen Lujia,Chen Meijuan,Zhou Yuping,Ye Changsheng,Liu Ruiyuan
Preparation of near-infrared (NIR) emissive fluorophore for imaging-guided PDT (photodynamic therapy) has attracted enormous attention. Hence, NIR photosensitizers of two-photon (TP) fluorescent imaging and photodynamic therapy are highly desirable. In this contribution, a novel D-π-A structured NIR photosensitizer (TTRE) is synthesized. TTRE demonstrates near-infrared (NIR) emission, good biocompatibility, and superior photostability, which can act as TP fluorescent agent for clear visualization of cells and vascular in tissue with deep-tissue penetration. The PDT efficacy of TTRE as photosensitizer is exploited in vitro and in vivo. All these results confirm that TTRE would serve as potential platform for TP fluorescence imaging and imaging-guided photodynamic therapy.
研究领域:
生物医学工程
·
分子影像
·
诊疗学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Qian Zhou,Huitian Du,Zhen Chen,Yuan Yu,Junfeng Ren,Shenghao Han,Jihui Fan,Zhiyong Pang
Near-infrared two-photon absorption (TPA) molecules designed based on intramolecular charge-transfer (CT) interactions have been widely investigated during the last few decades; however, studies on supramolecular TPA materials based on intermolecular CT interactions are quite rare. In this work, cocrystals of acridine and 1,2,4,5-tetracyano-benzene were successfully prepared by self-assembly. The cocrystals show yellow emission centered at ∼540 nm with a solid-state photoluminescence quantum yields (PLQY) of 22.48%. Two-photon excited fluorescence (TPEF) measurements show that the cocrystals display wide-window TPEF that is absent in their individual components. Optical absorption analysis indicate that the fluorescence is originated from excitation from the ground states to CT exciton states instead of exciton states of donor or acceptor molecules. This work may facilitate further two-component nonlinear optical material design and applications.
研究领域:
材料科学
·
光学
·
化学
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产品关联
DOI
高影响因子
Materials Horizons
·
2018
·Vol. 6
·期 3
·546-553
·
IF 13.183
Bo Situ,Meng Gao,Xiaojing He,Shiwu Li,Bairong He,Fengxia Guo,Chunmin Kang,Shan Liu,Lei Yang,Meijuan Jiang,Yanwei Hu,Ben Zhong Tang,Lei Zheng
High-resolution imaging of lipids within the artery is of great value to obtain fundamental knowledge about their roles in atherosclerosis, but ideal probes are still lacking. In this study, we report a smart probe, namely, IND with aggregation-induced emission property for two-photon visualization of lipids within cell lines and arteries. Taking advantage of high lipid specificity, deep tissue penetrability, and excellent two-photon imaging performance, IND is capable of high-resolution imaging of lipids in cultured cells and in situ mapping of three-dimensional lipid distributions in mouse atherosclerotic plaques without tedious histological preparations. Importantly, we find that IND exhibits distinct emissive properties in the molecular monomer and dimer states; also, we uncover its remarkable applications for single-excitation dual-color imaging of lipid/water in the tissue microenvironment to reveal the ultra-structure of atherosclerotic plaques. The findings described herein provide a simple tool with minimal disruptive procedures for studying atherosclerosis at the tissue level and a powerful strategy for the development of imaging-based diagnostic techniques with color switching capability.
研究领域:
生物医学工程
·
成像科学
·
心血管疾病
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Tingting Yang,Junyong Sun,Wu Yao,Feng Gao
To understand the relationship between endoplasmic reticulum (ER) stress and the level of hypochlorous acid (HOCl), it is highly desired to develop an ER-targetable HOCl probe to monitoring the HOCl level. In this study, a new turn-on type of two-photon ER-targetable fluorescent probe (ER-NPA) is designed for fluorescent sensing and imaging HOCl in ER using 4-hydroxy-1,8-naphthalimide as the fluorophore , p-aminophenyl ether as the recognition receptor, and methyl sulfonamide as ER-targetable group. The probe ER-NPA exhibits some intrinsic features such as excellent water solubility, good biocompatibility , significantly fast and enhanced fluorescence response to HOCl within 60 s, and detection limit as low as 6.2 nM for HOCl determination. The probe ER-NPA is successfully applied to real-time monitoring of exogenous and endogenous HOCl fluctuations in HeLa cells and zebrafish during ER stress. The developed probe ER-NPA may provide a new strategy to reveal the chemical biology roles of HOCl in ER stress-related diseases.
研究领域:
生物化学
·
医学
·
分子影像学
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产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 27
·16730-16736
·
IF 3.108
Ruiqing Feng,Longlong Li,Bing Li,Jinhui Li,Dan Peng,Yitao Yu,Qiuhong Mu,Ning Zhao,Xiaoqiang Yu,Zhenhua Wang
We have synthesized two-photon organic molecule probes (IMT-E and IMT-M), which selectively stain endogenous RNA in the nucleolus and cytoplasm of living cells in a short incubation time. Using these probes, bright two-photon excitation fluorescence (TPEF) images of living cells have been developed. Titrations and digest tests of ribonuclease indicate the markedly higher affinity of these probes for RNA, especially when using IMT-E. Upon binding to RNA, the fluorescence intensity of IMT-E increases by about 15-fold, showing that IMT-E is a turn-on probe for the detection of RNAs. MTT assays demonstrate that the mitochondria of cells maintain their electron mediating ability after being stained with the probes. These results demonstrate that IMT-E is an attractive two-photon turn-on fluorescent probe for visualizing RNA in living cells.
研究领域:
生物化学
·
分子生物学
·
细胞生物学
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 64
·36910-36914
·
IF 2.936
Long Xu,Yan Meng,Caixia Xu,Ping Chen
Solution-processed organic–inorganic halide lead perovskites have attracted increasing attention due to their great potential in low-cost, effective, and versatile light emission applications and large-scale portable optoelectronic devices. In this paper, formamidinium lead tribromide perovskite thin films composited with polyethylene oxide (PEO) were fabricated by a solution processing method. Great enhancement of photoluminescence was observed and more attractively, two-photon-pumped random lasing action could be achieved at room temperature when pumped by a nanosecond pulse laser with excitation wavelength centered at 1064 nm. Evident transition from spontaneous upconversion emission to random lasing action was investigated by monitoring the log–log light emission slope and peak width at half height. The lasing threshold is at around 1.1 mJ cm−2, which is comparable to that of other two-photon upconversion random lasers. The efficient random lasing emission originates from the multiple random scattering provided by the micrometer-scale rugged morphology and polycrystalline grain boundaries. Compared with conventional lasers that normally serve as a coherent light source, the perovskite random lasers show promise in fabricating low-cost thin-film lasing devices for flexible and speckle-free imaging applications.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Cheng-Yu Li,Di Cao,Ya-Feng Kang,Yi Lin,Ran Cui,Dai-Wen Pang,Hong-Wu Tang
We present an analytical platform by combining near-infrared optical tweezers with two-photon excitation for fluorescence detection of H5N1 virus gene sequences. A heterogeneous enrichment strategy, which involved polystyrene (PS) microsphere and quantum dots (QDs), was adopted. The final hybrid-conjugate microspheres were prepared by a facile one-step hybridization procedure by using PS microspheres capturing target DNA and QDs tagging, respectively. Quantitative detection was achieved by the optical tweezers setup with a low-cost 1064 nm nanosecond pulse laser for both optical trapping and two-photon excitation for the same hybrid-conjugate microsphere. The detection limits for both neuraminidase (NA) gene sequences and hemagglutinin (HA) gene sequences are 16–19 pM with good selectivity for one-base mismatch, which is approximately 1 order of magnitude lower than the most existing fluorescence-based analysis method. Besides, because of the fact that only signal from the trapped particle is detected upon two-photon excitation, this approach showed extremely low background in fluorescence detection and was successfully applied to directly detect target DNA in human whole serum without any separation steps and the corresponding results are very close to that in buffer solution, indicating the strong anti-interference ability of this method. Therefore, it can be expected to be an emerging alternative for straightforward detecting target species in complex samples with a simple procedure and high-throughput.
研究领域:
生物技术
·
分子生物学
·
光学分析
✓ 已验证
DOI
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 22
·36037-36047
·
IF 3.8
Dexing Zhu,Jian Zhang,Qiao Xu,Yaguo Li
Three-dimensional printing enables the fabrication of silica glass optics with complex structures. However, shrinkage remains a significant obstacle to high-precision 3D printing of glass optics. Here we 3D-printed Dammann gratings (DGs) with low lateral shrinkage (<4%) using a two-photon polymerization (2PP) technique. The process consists of two steps: patterning two-photon polymerizable glass slurry with a 515 nm femtosecond laser to form desired structures and debinding/sintering the structures into transparent and dense silica glass. The sintered structures exhibited distinct shrinkage rates in the lateral against longitudinal directions. As the aspect ratio of the structures increased, the lateral shrinkage decreased, while the longitudinal shrinkage increased. Specifically, the structure with an aspect ratio of approximately 60 achieved a minimal lateral shrinkage of 1.1%, the corresponding longitudinal shrinkage was 61.7%. The printed DGs with a surface roughness below 20 nm demonstrated good beam-shaping performance. The presented technique opens up possibilities for rapid prototyping of silica diffractive optical elements.
研究领域:
光学
·
材料科学
·
制造技术
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2024
·Vol. 36
·期 31
·2403775
·
IF 27.4
Yupeng Liu,Dengke Cheng,Bingzhe Wang,Junxiang Yang,Yiming Hao,Jing Tan,Qijun Li,Songnan Qu
Achieving thermochromic afterglow (TCAG) in a single material for advanced information encryption remains a significant challenge. Herein, TCAG in carbon dots (CDs)-inked paper (CDs@Paper) is achieved by tuning the temperature-dependent dual-mode afterglow of room temperature phosphorescence (RTP) and thermally activated delayed fluorescence (TADF). The CDs are synthesized through thermal treatment of levofloxacin in melting boric acid with postpurification via dialysis. CDs@Paper exhibit both TCAG and excitation-dependent afterglow color properties. The TCAG of CDs@Paper exhibits dynamic color changes from blue at high temperatures to yellow at low temperatures by adjusting the proportion of the temperature-dependent TADF and phosphorescence. Notably, two-photon afterglow in CDs-based afterglow materials and time-dependent two-photon afterglow colors are achieved for the first time. Moreover, leveraging the opposite emission responses of phosphorescence and TADF to temperature, CDs@Paper demonstrate TCAG with temperature-sensing capabilities across a wide temperature range. Furthermore, a CDs@Paper-based 3D code containing color and temperature information is successfully developed for advanced dynamic information encryption.
研究领域:
材料科学
·
光学
·
信息加密技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Chenglin Zheng,Feng Jin,Yuanyuan Zhao,Meiling Zheng,Jie Liu,Xianzi Dong,Zhong Xiong,Yanzhi Xia,Xuanming Duan
Light-driven micro/nano-actuators are one of the most important topics in the biomedical micro-electromechanical systems (MEMS) field. Currently, their development is hampered due to the difficulties in designing and fabricating biocompatible light-driven microactuators with the dimensions less than one hundred micrometres and a response time in the order of seconds. In this work, gel photoresists were prepared by embedding photothermal surface-modified Fe 3 O 4 nanoparticles (NPs) into a mixture that included a photoinitiator, photosensitizer, monomers, crosslinkers and solvents. Macroscopic poly( N -isopropylacrylamide) (PNIPAM)/nano-Fe 3 O 4 hydrogels were prepared by ultraviolet photopolymerization of gel photoresists, which showed good temperature-responsive volume changes and light-triggered bending deformation. Then the two-photon polymerization (TPP) microfabrication properties of gel photoresists with 0, 0.48 and 0.95 wt% Fe 3 O 4 NPs were investigated in detail. Importantly, after the TPP microfabrication and subsequent solvent-exchange procedure, a double-armed near-infrared (NIR)-light-driven three-dimensional (3D) hydrogel microcantilever with a size of ∼26 μm was successfully fabricated. The hydrogel microactuator had a fast response time of ∼0.033 s in water under NIR radiation and showed good reversibility. Furthermore, the distance between the two arms of the hydrogel microcantilever could be manipulated by controlling the laser focus and incident laser power.
研究领域:
生物医学工程
·
材料科学
·
微机电系统(MEMS)
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Wanying Li,Bingqing Fang,Ming Jin,Yang Tian
Zinc ion (Zn2+) not only plays an important function in the structural, catalytic, transcription, and regulatory of proteins, but is also an essential ionic signal to regulate brain neurotransmitters pass process. In this work, we designed and synthesized an intramolecular charge transfer-based ratiometric two-photon fluorescence probe, P–Zn, for imaging and biosensing of Zn2+ in live cell, hippocampal tissue, and zebrafish. The developed probe demonstrated high two-photon absorption cross section (δ) of 516 ± 77 GM, which increased to 958 ± 144 GM after the probe was coordinated with Zn2+. Furthermore, this P–Zn probe quickly recognized Zn2+ with high selectivity, over other metal ions, amino acids, and reactive oxygen species. More interestingly, the initial emission peak of the present probe at 465 nm decreased with a new peak increased at 550 nm, leading to the ratiometric determination of Zn2+ with high accuracy. Finally, this two-photon fluorescence probe with high temporal resolution and remarkable analytical performance, as well as low-cytotoxicity, was successfully applied in imaging of live cells, hippocampal tissues, and zebrafishes. The present P–Zn probe combined with FLIM provided accurate mapping of Zn2+ distribution at single-cell level. More interestingly, the two-photon spectroscopic results demonstrated that the level of Zn2+ in hippocampal tissue of mouse with AD was higher than that in normal mouse brain.
研究领域:
生物化学
·
神经科学
·
医学影像
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 47
·18966-18976
·
IF 7.367
Dong-Yang Zhang,Yue Zheng,Hang Zhang,Liang He,Cai-Ping Tan,Jing-Hua Sun,Wei Zhang,Xingyun Peng,Qiuqiang Zhan,Liang-Nian Ji,Zong-Wan Mao
Nanohybrids can in most cases kill cancer cells more efficiently as compared with free photosensitizers. In this work, we constructed nanohybrid Ru1@CDs composed of carbon nanodots (CDs) and a phosphorescent Ru(II) complex (Ru1) for one- and two-photon photodynamic therapy of cancer. The photosensitizer and imaging agent Ru1 is decorated onto the nanocarrier CDs covalently. Ru1 and Ru1@CDs can penetrate into cancer cells through an energy-dependent mechanism and endocytosis, respectively. Both Ru1 and Ru1@CDs are capable of lysosome-targeted phosphorescence imaging and photodamage under either 450 nm (one-photon) or 810 nm (two-photon) excitation. Conjugation with CDs can increase the cellular uptake efficacy of Ru1. Mechanism investigations show that both Ru1 and Ru1@CDs can induce apoptosis through generation of reactive oxygen species and cathepsin-initiated apoptotic signaling pathways. Upon two-photon excitation, Ru1@CDs show better penetrability, as well as higher inhibitory effects on cancer cell growth in both 2D cell and 3D multicellular tumor spheroid models. Our work provides an effective strategy for the construction of multifunctional imaging and phototherapeutic nanohybrids for the treatment of cancer.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）