Liu Zhi-Min,Zheng Ying-Ying,Jing Yan-Feng,Li Jie,Zhan Hai-Jun,Shen Qi
A facile, one-step synthesis of ionic liquid-functionalized graphene (IL-GR) nanocomposite using graphene oxide (GO) and amine-terminated ionic liquid (NH2-IL) was first presented through an epoxide ring-opening reaction, then acetylcholinesterase (AChE) was immobilized onto
IL-GR nanocomposite film modified glassy carbon electrode to prepare a highly sensitive AChE biosensor. The morphologies and electrochemistry properties of the composite were investigated by using scanning electron microscopy, transmission electron microscopy and cyclic voltammetry, respectively.
The nanocomposite not only provided a biocompatible microenvironment to keep the bioactivity of AChE, but also exhibited a strong synergetic effect on improving the sensing properties of phoxim. The immobilized AChE showed favorable affinity to acetylthiocholine (ATCl) and could catalyze the
hydrolysis of ATCl with a Kapp
m
value of 0.143 mM. The proposed biosensor exhibited a detection limit of 6.0 × 10–11 M (S/N = 3) towards phoxim. Besides, the biosensor is easy to prepare, have good stability, and will have potential application
in organophosphate pesticides.
Fungal diseases caused by Botrytis cinerea and insect infestations by aphids often occur simultaneously during plant growth. A co-delivery nanosystem for dual-pesticide may enhance pesticide efficiency and reduce the required dosage of pesticides through synergistic effects. Therefore, in this study, we developed a dual pH-responsive nanocomposite AZOX/Thi@BMMs@MIL-88B (ATBM) based on bimodal mesoporous silica (BMMs) and metal organic framework MIL-88B, which was loaded with fungicide azoxystrobin (AZOX) and insecticide thiamethoxam (Thi) to control grey mould and aphid pests. The loading capacities of the two pesticides in the nanocomposite were 18.56 % (AZOX) and 37.38 % (Thi). The nanoparticles (NPs) were spindle-shaped with a hydrated particle size of 771.60 ± 12.55 nm and possessed excellent pH-responsive controlled release behavior. The NPs also demonstrated good wettability and stability due to the entrapment of nanocarriers. Fluorescence tracking experiments verified that the NPs could be effectively absorbed and translocated by pathogenic fungal mycelium and pea seedlings. The bioactivities of ATBM against B. cinerea and A. pisum increased by 9.58 % (AZOX) and 39.48 % (Thi), respectively, compared to the commercialized technical control (TC). This nanocomplex promoted the growth of pea seedlings through the introduction of iron ions and by activating the oxidative stress system, and the excellent biosafety of NPs could minimize environmental risk. In summary, this hybrid bi-carrier dual-pesticide nanopesticide-fertilizer showed significant promise for application in sustainable agriculture.
危险品化学品经营许可证（带存储）