INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 8
·7111
·
IF 5.6
Yanyun Lin,Xingjuan Wu,Kaicheng Wang,Sen Shang,Yubin Gong,Hongwei Zhao,Dai Wu,Peng Zhang,Xiaoyun Lu
Understanding the vibrational information encoded within the terahertz (THz) spectrum of biomolecules is critical for guiding the exploration of its functional responses to specific THz radiation wavelengths. This study investigated several important phospholipid components of biological membranes—distearoyl phosphatidylethanolamine (DSPE), dipalmitoyl phosphatidylcholine (DPPC), sphingosine phosphorylcholine (SPH), and lecithin bilayer—using THz time-domain spectroscopy. We observed similar spectral patterns for DPPC, SPH, and the lecithin bilayer, all of which contain the choline group as the hydrophilic head. Notably, the spectrum of DSPE, which has an ethanolamine head group, was different. Interestingly, density functional theory calculations confirmed that the absorption peak common to DSPE and DPPC at approximately 3.0 THz originated from a collective vibration of their similar hydrophobic tails. Accordingly, the cell membrane fluidity of RAW264.7 macrophages with irradiation at 3.1 THz was significantly enhanced, leading to improved phagocytosis. Our results highlight the importance of the spectral characteristics of the phospholipid bilayers when studying their functional responses in the THz band and suggest that irradiation at 3.1 THz is a potential non-invasive strategy to increase the fluidity of phospholipid bilayers for biomedical applications such as immune activation or drug administration.Keywords:phospholipid bilayers;THz absorption spectra;cell membrane fluidity;phagocytosis
研究领域:
生物物理学
·
医学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2014
·
IF 4.287
Zhengpeng Yang,Chunjing Zhang,Lina Huang
The planar and rough phospholipid bilayers at the surfaces of quartz crystal and titania-modified quartz crystal were fabricated via the surface modification, respectively, and characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and piezoelectric measurement. The formation of calcium phosphate on planar and rough phospholipid bilayers was investigated in detail using in situ quartz crystal microbalance (QCM) and X-ray diffraction (XRD) techniques. The obtained results showed that the calcium phosphate precipitation was closely related to the roughness and surface potential of phospholipid bilayers. Compared with planar phospholipid bilayers, the rough phospholipid bilayers exhibited a higher deposition rate of calcium phosphate. The presence of anionic phosphatidylserine (PS) in phosphatidylcholine (PC)/PS phospholipid induced PC/PS surface with negative charge, thus showing significantly enhanced calcium phosphate precipitation.
研究领域:
生物材料学
·
生物物理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ao Huang,David Julian McClements,Xiuting Hu,Shunjing Luo,Chengmei Liu,Jiangping Ye
This study prepares food-grade nanodiscs using Tween 80 and lecithin. Initially, the formation process of the nanodiscs was observed, starting with the transformation of the hollow vesicle structure of lecithin into a notched vesicle structure, followed by the development of solid core nanodiscs, and ultimately into a cylindrical structure. The results from ITC indicated that hydrophobic interaction was the primary driving force behind the self-assembly of the nanodiscs with the binding ratio of lecithin to Tween 80 being 10:1. Owing to curvature effects, lecithin self-assembled into planar regions, while Tween 80 self-assembled into the edge regions. In comparison to hydrated liposomes, nanodiscs exhibited a smaller size, along with significant changes in molecular ordering, surface hydrophobicity, and microenvironment polarity of the phospholipid bilayers. Additionally, nanodiscs demonstrated superior encapsulation properties. This study elucidated the self-assembly process and structure of nanodiscs using food-grade ingredients, emphasizing their potential as delivery systems for food applications.
研究领域:
食品科学
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纳米技术
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胶体化学
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药物递送
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生物材料
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分子自组装
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Shaoying Dai,Tengda Wang,Jie Cui,Junfeng Xiang,Qing Shao,Yuchun Han,Yilin Wang
Seeking effective ways to maintain cellular homeostasis is crucial to the survival of organisms when they encounter osmotic stress. Glycine betaine (GB) is a widely generated natural osmolyte, but its endogenous production and action are limited. Herein, a kind of nonionic surfactant dodecyl-β-d-glucopyranoside (DG) and a common polymer polyethylene glycol (PEG) are proven to have the ability to enhance the osmotic stress (induced by sugar concentration changes) tolerance of cell and organism models, those are giant unilamellar vesicles (GUVs) and gram-negative Escherichia coli . DG or PEG only induces small size decrease and certain shape change of GUVs. Importantly, DG or PEG at the concentration 100 times lower than that of GB effectively increases the survival rate of bacteria under both hypoosmotic and hyperosmotic conditions. This intriguing result is attributed to the insertion of DG or adsorption of PEG in the lipid bilayer membrane, leading to enhanced membrane permeability. These exogenous substances can replace GB to facilely and highly efficiently augment adaptation of organisms to osmotic stress.
研究领域:
细胞生物学
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微生物学
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生物物理学
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DOI
PubMed
高影响因子
Nanomaterials
·
2025
·Vol. 15
·期 18
·1421
·
IF 4.3
Qin Li,Jian Chen,Xingwu Zhang,Chenjie Ruan,Weiwei Wu
With the rapid growth of nuclear energy, effective shielding of radioactive nuclear by-products is critical for safety and environmental protection. Gadolinium (Gd) is ideal for neutron shielding due to its exceptionally high thermal neutron capture cross-section. Despite significant progress in developing various Gd-based shielding materials, poor interfacial compatibility between Gd2O3and polymer matrices remains a significant limitation. In this study, we addressed this challenge by successfully modifying Gd2O3nanoparticles (Gd2O3@SIT-M) through the construction of a dual-layer molecular coating using electrostatic interactions. Initially, Gd2O3was functionalized with the silane coupling agent 3-(trihydroxysilyl) propyl-1-propane-sulfonic acid (SIT), followed by subsequent assembly of polyether amine M2070 onto this modified surface. The combined presence of hydrophilic sulfonic acid groups from SIT and amine-ether groups from M2070 endowed Gd2O3@SIT-M nanoparticles with excellent hydrophilicity, significantly reducing their aqueous contact angle to 14.34°. Consequently, this modification strategy notably enhanced the dispersion stability of Gd2O3nanoparticles in aqueous solutions and polymer matrices. The developed approach thus provides an effective pathway for fabricating advanced polymer-based neutron shielding materials with improved dispersibility, stability, and overall performance.
研究领域:
材料科学
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核工程
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纳米技术
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高分子化学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yibang Zhang,Dong Chen,Yang Liu,Aisha Labaran Ammani,Atena Ahmadian,Aftab Ullah
Protein-lipid interactions are pivotal in various biological processes, including membrane trafficking, signal transduction, and cellular compartmentalization. This study investigates how fatty acid modification alters the physicochemical properties of ovalbumin (OVA) and its interactions with lipid bilayers, with implications for controlled release applications. We hypothesize that modifying OVA with different fatty acids will significantly alter its surface hydrophobicity and membrane affinity, thereby influencing its potential for targeted drug delivery. OVA was chemically modified with four fatty acids (myristic, palmitic, stearic, and oleic acids) using N-hydroxysuccinimide (NHS) esters. Surface hydrophobicity was assessed via 8-Anilino-1-naphthalenesulfonic acid ammonium (ANS) fluorescence assays, revealing that modifications increased hydrophobicity, particularly with longer fatty acid chains. Interactions with liposomes were characterized using dynamic light scattering (DLS) and zeta potential measurements, indicating enhanced liposome binding and altered bilayer properties due to fatty acid modifications. Quartz crystal microbalance with dissipation (QCM-D) analysis confirmed the insertion of fatty acid chains into the lipid bilayer. In vitro release experiments demonstrated that fatty acid modification significantly affected the release kinetics of OVA, with oleic acid-modified OVA showing the lowest cumulative release. Our findings highlight the role of fatty acid structure in modulating protein-lipid interactions and underscore the potential of fatty acid-modified OVA for developing targeted drug delivery systems. Future work will explore the underlying mechanisms and evaluate the therapeutic potential of these modified proteins in vivo.
研究领域:
生物物理学
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生物化学
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药学
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分子生物学
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胶体与界面科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）