SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Xin Shang,Jiayang He,Chunli Yang,Siyu Zuo,Ruo Yuan,Wenju Xu
Development of logic AND-gated multivariate assay systems of DNA-scaffolded green- and red-fluorescent Ag nanoclusters ( g- AgNC and r- AgNC) is promising and remains challenging. By using two DNA segments ( T o and T r) as dual-targeting inputs, herein we report an innovative strategy that utilizes targets-recycled amplification to create a sensitive AND-gated light-up ratiometric sensor with reversely changed fluorescence of g- AgNC and r- AgNC. The design is based on that: two hairpins ( H o and H r) are individually recognizable to T o and T r, and two hybridized duplexes are resultantly implemented for transient dissipative base pairing, i.e. H o· H r, releasing T o and T r for repeated recycling. In an original stem-loop hairpin reporter (HR), one exposed overhang is to host g -AgNC. When HR is unfolded by the cooperative sticky toeholds in H o∙ H r, its liberated stem merging with the g -AgNC template is for r- AgNC clustering. Only when meeting simultaneously the overexpressed T o and T r in SARS-CoV-2, the AND-gated ratio fluorescence of r- AgNC over g- AgNC can be lighted-up in a typical assay, enabling highly specific-inputs binding and sensitive dose-dependence down to 0.29 pM. This enzyme-free and label-free AND-logic ratiometirc strategy illustrates the potential of using multicolor AgNCs emitters for more accurate multivariate assay.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
ACS Nano
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2024
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IF 15.8
Xinru Yuan,Jia-Xin Wang,Yunlong Li,Hao Huang,Jiahong Wang,Tongyu Shi,Yuhao Deng,Qiyu Yuan,Rui He,Paul K. Chu,Xue-Feng Yu
Increasing modal variations of stimulus-responsive materials ensure the high capacity and confidentiality of information storage and encryption systems that are crucial to information security. Herein, thermochromic perovskite microcapsules (TPMs) with dual-variable and quadruple-modal reversible properties are designed and prepared on the original oil-in-fluorine (O/F) emulsion system. The TPMs respond to the orthogonal variations of external UV and thermal stimuli in four reversible switchable modes and exhibit excellent thermal, air, and water stability due to the protection of perovskites by the core–shell structure. Benefiting from the high-density information storage TPMs, multiple information encryptions and decryptions are demonstrated. Moreover, a set of devices are assembled for a multilevel information encryption system. By taking advantage of TPMs as a “private key” for decryption, the signal can be identified as the corresponding binary ASCII code and converted to the real message. The results demonstrate a breakthrough in high-density information storage materials as well as a multilevel information encryption system based on switchable quadruple-modal TPMs.
研究领域:
材料科学
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信息安全
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Chunli Yang,Yanan Shi,Yuqing Zhang,Jiayang He,Zhihan Zhang,Xinyue Jia,Ruo Yuan,Wenju Xu
Constructing label-free bivariate fluorescence biosensor would be intriguing and desired for the recognizable and accurate detection of two specific DNA segments, yet the design of functional DNA structures with low overlapped interference might be challenging. Herein in this work, a double-faced J anus D NA n ano a rchitecture ( J DNA) with bi-responsive recognition regions on opposite sides was assembled, which consisted of two substrate strands and two template strands for loading green-/red-emissive Ag nanoclusters ( g AgNC and r AgNC) as bivariate signaling reporters. Of note, the hybridized double helix in the middle rationally oriented two flank faces and stabilized the rigid conformation of J DNA, while the template sequences of bicolor clusters were blocked to minimize non-specific background leakage. Upon inputting two targets, the discernible hairpins lost their hairpin structures due to forming two dsDNA complexes. They were executed to simultaneously invade J DNA for activating two individual target-recycled strand displacement (TRSD) events, guiding signal transduction and efficient amplification. Consequently, the clustering templates were unlocked via the tailored conformation switch of J DNA, in which g AgNC and r AgNC were in situ synthesized in two diagonal positions, thereby significantly emitting bi-responsive signal without cross interference. Benefited from the logic integration of double-faced J DNA and TRSD, a label-free, sensitive and specific bivariate fluorescence approach was developed, which would open a new avenue for the potential application in biosensing and bioanalysis.
研究领域:
生物化学
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纳米生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Zhengchao Ji,Beihan Sun,Ting Yang,Xuan Li,Zhenwei Zhang,Mengfan Bao,Liping Zhao,Hongyin Lou,Yanyan Li,Guoxiang Sun,Jing Huang
Ethnopharmacological relevance YiXinShu capsule (YXSC), originally from the classical TCM formula named “Sheng-Mai-San”, has been extensively utilized in clinic for the treatment of cardiovascular diseases. However, there were few reports about the quality assessment of YXSCs both internationally and domestically. Aim of the study The objective was to develop a multi-strategy platform incorporating systematic quantitative fingerprint analysis and antioxidant activity determination, with chemometric analysis and bivariate correlation analysis as the auxiliary approaches, to assess and monitor the quality of YXSCs. Materials and methods Firstly, according to the Chinese Pharmacopoeia (2020 edition), 12 key indicator components from seven herb medicines were quantified by HPLC method. Then, three-dimensional fingerprints comprising five-wavelength fusion fingerprint (FWF-FP), electrochemical fingerprint (EC-FP) and Differential Scanning Calorimetry fingerprint (DSC-FP) were established to assess and monitor YXSCs using systematically quantified fingerprint method (SQFM) and principal component analysis (PCA). Moreover, by integrating the analysis of the three-dimensional fingerprints, the quality of YXSCs from different batches was effectively screened. Finally, the antioxidant activity of this TCM was assessed through DPPH and ABTS methods, and the L-ascorbic acid equivalent antioxidant capacity (AEAC) values were compared to evaluate the antioxidant activities of the two methods. A Partial Least Squares (PLS) model was used to develop the spectrum-activity relationship between FWF-FP and AEAC, and a bivariate correlation analysis (BCA) was used to assess the correlation between FWF-FP and EC-FP. Results The key indexes including tanshinone I, t ol , t oe , A tp , first exothermic peak, and second exothermic peak can differentiate between various batches of YXSCs based on their three-dimensional fingerprint profiles. The integration evaluation results from 42 batches of YXSCs were categorized into 2–5 grades, indicating good quality consistency across different batches. In vitro studies have indicated a significant antioxidant activity capacity of YXSCs. The PLS model revealed that 37 out of the 41 fingerprint peaks exhibited antioxidant activity. The overall trend of BCA was consistent with PLS model results. Conclusion This research presents a scientific and holistic strategy for the quality consistency evaluation of YXSCs, thereby offering an effective approach for the thorough evaluation of TCMs.
研究领域:
药理学
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中药学
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分析化学
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质量控制
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生物统计学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）