Nanoscale
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2022
·Vol. 14
·期 21
·7807-7816
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IF 8.307
Hongyu Du,Yunqing Wang,Panpan Zhang,Rongchao Mei,Yunxia Ji,Xizhen Zhao,Zhiyang Zhang,Jiping Ma,Lingxin Chen
Nanoplastics (NPs) as emerging marine pollutants can be taken up by seafood organisms. It is crucial to quantitatively assess NP's distribution behavior in organisms to elucidate concentration dependent biological effects. Such a knowledge gap has remained due to the lack of reliable NP models and analytical methods. Herein, surface enhanced Raman scattering (SERS)-labeled NP models were developed and their bioavailability, distribution and accumulation in Ruditapes philippinarum, a typical marine bivalve, were quantitatively studied. Taking advantage of the sensitive and characteristic SERS signals of the NP models, distribution could be quickly and accurately obtained by the Raman imaging technique. Moreover, quantitative analysis of NPs could be performed by the detection of gold element contents via inductively coupled plasma mass spectroscopy (ICP-MS) detection. ICP-MS results revealed that after 3 days exposure of monodispersed NPs (100 nm, 0.2 mg L−1), the digestive gland accumulated 86.7% of whole-body NPs followed by gill (5.2%), mantle (5.1%), foot (1.3%), exhalant siphon (1.1%), and adductor (0.6%). Upon 11 days depuration, 98.7% of NPs in the digestive gland were excreted, whereas the clearance ratios in other organs were much lower. NP aggregates (around 1.5 μm) demonstrated similar distribution and clearance trends to the monodispersed ones. However, the accumulation amount in each organ was 15.2% to 77.6% lower. Surface adherence and passive ingestion routes resulted in NP accumulation, which contributed to the comparable NP abundance in these organs. Additionally, boiling treatment (mimicking a cooking process) did not decrease the NP amount in these organs. This work provided a dual-mode and quantitative analysis protocol for NPs for the first time, and suggested the risk of NP uptake by humans via bivalve seafood diets.
研究领域:
环境科学
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海洋生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Wenting Li,Xinai Zhang,Xuetao Hu,Yongqiang Shi,Zhihua Li,Xiaowei Huang,Wen Zhang,Di Zhang,Xiaobo Zou,Jiyong Shi
A novel fluorescence sensing platform was fabricated for visual detection of cadmium ions (Cd 2+ ) with excellent stability and portability. In this protocol, dual-emission ratiometric fluorescence probe were constructed based on silicon oxide-coated copper nanoclusters (CuNCs@SiO 2 ) as a signal reference and cadmium telluride quantum dots (CdTe QDs) as signal response, thereby greatly improving the accuracy of test results. The level of Cd 2+ can be reported within a wide linear range from 0.010 mg·L −1 to 2.0 mg·L −1 with a sensitive detection limit of 1.1 μg·L −1 (2.75 μg·kg −1 ) and a quick sample-to-answer monitoring time of 6 min, which was quite qualified for regularly monitoring Cd 2+ . Moreover, aiming to attain portable analysis, the smartphone as colorimetric reader and analyzer were also utilized for rapidly analyzing Cd 2+ by capturing the change in fluorescence color. Additionally, benefiting from the strong combination of 1, 10-phenanthroline (Phen) and Cd 2+ , the fluorescence probe showed excellent anti-interference activities for Cd 2+ assay in complex oyster matrix. Overall, the sensing platform had significant stability, specificity and sensitivity, offering a promising potential for conveniently evaluating the quality of marine bivalves polluted with Cd 2+ .
研究领域:
分析化学
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环境科学
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生物技术
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 13
·19890-19897
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IF 5.19
Zhao Anran,Miao Jingjing,Liu Liru,Pan Luqing
Retinoid X receptor (RXR) has been found to be a major target in various processes of endocrine disruption from the exposure to organotin compounds (OTCs), including imposex in gastropod mollusks. It was also reported in bivalves that OTCs caused intersex and skewed sex ratio. In order to evaluate the effect of these ligand-like OTCs, we constructed a reconstituted yeast system (CfRE system) based on GAL4 yeast two-hybrid principle using scallop Chlamys farreri retinoid X receptor ( Cf RXR a ) and retinoid X response element (RXRE) to investigate the ligand-induced transactivation of Cf RXR a . Responses of Cf RXR a to 9-cis retinoic acid (9cRA) and tested four OTCs showed concentration-dependent response which is comparable with reported RXRa in vitro assay of human and gastropods. The detective limits of the CfRE system were found to be 100 nM for 9cRA and 10–1000 nM for the tested OTCs. While the tested non-Sn endocrine disrupting chemicals, including Benzo[a]pyrene, 2,4-Dichlorophenol, Nonylphenol, and Tetrabromobisphenol A, showed no effect on Cf RXR a response. The present assay system may provide a valuable tool for screening assessments of unidentified environmental ligand chemicals on bivalve mollusks. It is also useful for comparison of sensitivity differences among species exposed to EDCs.
研究领域:
分子生物学
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环境科学
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毒理学
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2021
·
IF 4.964
Wei Shi,Shuge Sun,Yu Han,Yu Tang,Weishang Zhou,Weixia Zhang,Xueying Du,Lin Huang,Guangxu Liu
By employing external fertilization (broadcast spawning) as a mating strategy, the gametes and subsequent fertilization of various marine invertebrates are directly subjected to pollution. Although microplastics (MPs) are ubiquitous in marine environments, their potential effects on the fertilization of broadcast spawners remain largely unknown. Therefore in this study, the impacts of polystyrene MPs on the fertilization success of broadcast spawning bivalve ( Tegillarca granosa ) were investigated. In order to reveal the underlying mechanisms affecting fertilization, the sperm swimming performance, sperm ATP status, sperm viability, DNA integrity, gamete collision probability, gamete fusion efficiency, enzymatic antioxidants, and key ion transport enzyme activities were analyzed. The results showed that MPs weakened the sperm swimming performance through reducing ATP production and cell viability, thus leading to the decreased probability of gamete collision. Furthermore, MPs affected ion transport in the gametes by inducing oxidative stress, which resulted in gamete fusion failure. In conclusion, this study demonstrates that MPs could significantly decrease the fertilization success of T. granosa through reducing gamete collision and lowering gamete fusion efficiency.
研究领域:
海洋生物学
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环境科学
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生殖生物学
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产品关联
DOI
PubMed
高影响因子
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