FOOD CHEMISTRY
·
2025
·
IF 9.8
Jie Wu,Ling Wu,Mengmeng Guo,Wen Hao,Lin Cai,Guozhen Fang,Shuo Wang
Enrofloxacin (ENR) residues in animal derived foods are harmful to human health. A molecularly imprinted electrochemiluminescence sensor was constructed for ENR detection utilizing a self-enhanced quasi-three-dimensional luminescent-composite of boron nitride quantum dots/nitrogen-doped mesoporous carbon crosslinked with copper nanowires (BNQDs/NMC-CuNWs). One-dimensional CuNWs with near-infrared electrochemiluminescence (NIR-ECL) properties were inserted into the electroactive layer of two-dimensional NMC to form quasi-three-dimensional NMC-CuNWs, which inhibited the aggregation of CuNWs and increased the electron and active ion transport rates. BNQDs were also embedded into the NMC-CuNWs network as co-reactant accelerators and antioxidants. A molecularly imprinted polymer containing bifunctional monomers enabled the specific detection of ENR. The detection range of ENR was 1.00 × 10 −9 –5.00 × 10 −5 mol L −1 , with a low limit of detection (3.33 × 10 −10 mol L −1 ). The recovery rate of ENR was 83.92 %–94.56 %, which is consistent with the high-performance liquid chromatography results. This work offers new ideas for detecting hazardous substances in the NIR-ECL field.
研究领域:
食品安全检测
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Tao Lu,Zhiguang Suo,Min Wei,Huali Jin,Yong Liu,Baoshan He,Renyong Zhao
β-lactoglobulin (β-LG) is a major allergen in milk what can provoke intense allergic reactions. Therefore, an accurate and efficient method is required for sensitive detection of β-LG. A label-free electrochemical aptasensor utilizing Au/Ag@ZIF-8 and dual amplification strategy has been designed for the detection of β-LG. Au/Ag@ZIF-8 nanocomposite is used as the electrode modification material, which has abundant relative surface area and exceptional conductivity behavior, and it can also serve to immobilize DNA strands. By combining RecJf exonuclease and hybridization chain reaction (HCR) dual amplification strategy, an apparent enhancement of electrochemical signal is obtained. β-LG undergoes target cycling in the effect of RecJf exonuclease, which dislodges complementary strand cDNA and further triggers HCR to provide a large number of sites for methylene blue embedding. The detection range of this aptasensor is 0.01–1000 ng/mL in the optimal detection conditions, with a limit of detection (LOD) of 5.37 pg/mL. The constructed electrochemical aptasensor possesses the superiorities of good specificity, preeminent accuracy and satisfied stability in the detection of β-LG. Moreover, it also demonstrates satisfactory detection performance in the detection of real samples, which indicates that the electrochemical aptasensor has a broad application prospect in protecting food safety and health.
研究领域:
食品安全检测
·
生物传感技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Chi Zhang,Gaiping Li,Baoxian Ye,Lina Zou,Weihang Wang,Yanli Ji
Based on ExoIII-assisted nucleic acid circulation and catalytic hairpin assembly (CHA) reaction, a dual-amplification electrochemical biosensors strategy for sensitive detection of tobramycin was presented. When the target was present, the trigger 1 (T1) was released to unfold hairpin DNA (H1), which allowed the ExoIII-assisted nucleic acid circulation to proceed smoothly and create a lot of trigger 2(T2). Following that, the CHA reaction between H2 and H3 proceeded under the catalysis of T2 and produced plenty of H2-H3 complex. Signal probe labeled with ferrocene was immobilized on the electrode ahead by adenine (PolyA). Finally, the H2-H3 complex combined with the probe and the DNA strand labeled with ferrocene (C2) fell off the electrode surface. The above process significantly reduced the electrochemical signal, so the biosensor has a low detection limit of 0.11 nM. Moreover, this biosensor has a satisfactory regenerability.
研究领域:
生物化学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiao Fang Zhang,Na Li,Cui Ye,Jia Yu Liang,Nian Bing Li,Hong Qun Luo
Uracil-DNA glycosylase (UDG), one of the base-excision repair enzymes, is closely relevant for the diseases of the organism. Here, we proposed a resonance Rayleigh scattering (RRS) strategy for sensitively detecting the active UDG based on a dual amplification DNA machine without label under the catalysis of exonuclease III (Exo III). In our study, a double-stranded DNA complex S1-S2 containing triggers and peculiar uracil bases was employed. To achieve the dual amplification strategy, we adopted hairpin probes HP1 and HP2. The unique hairpin probes can partially hybridize with S1 and S2, respectively. With the excision reaction of UDG, the uracil bases were excised from S2 in double-stranded S1-S2, resulting in a lower melting temperature of the S1-S2. Then, the S1-S2 was dissociated to single-stranded S1 and S2 with abasic sites. Subsequently, the liberated strands separately hybridized with hairpins to initiate the Exo III-catalyzed dual amplification. In the presence of K + , countless c-myc sequences which were produced by the amplification reaction formed G-quadruplex structures. Finally, with the addition of Mg 2+ , long and continuous G-wire structures were obtained, causing an obvious enhancement of RRS intensity. The proposed RRS DNA machine obtained the limit of detection as low as 1.0 × 10 −5 U/mL for the active UDG assay. Moreover, qualitative analysis of the active UDG was successfully achieved in HeLa cells lysate. The DNA machine is a potential tool to be used in UDG clinical diagnosis or functional study.
研究领域:
分子生物学
·
分析化学
·
生物医学工程
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 57
·46301-46306
·
IF 3.84
Jing Fang Guo,Rong Mei Fang,Cheng Zhi Huang,Yuan Fang Li
In order to eliminate the scattered light induced by the signal amplifier in fluorescence anisotropy (FA) assays, a nanosized metal–organic framework MIL-101, ranging from 80–500 nm, has been synthesized through a hydrothermal method with the addition of glycerol. We chose the 100 nm MIL-101 to enhance FA for label-free detection of the respiratory syncytial virus (RSV) gene sequence and the DNA-intercalating dye SYBR Green I (SGI) as the fluorophore, based on the different affinities of MIL-101 toward ssDNA and dsDNA. The nanosized MIL-101 has a negligible scattering effect owing to its smaller particle size, so all of the experimental data of FA values were smaller than the maximum initial anisotropy of 0.4. As a specific advantage, a dual amplification result of not only an increase in the FA value of SGI/ssDNA (r1) but also a decrease in the FA value of SGI/dsDNA (r2) was presented at the same time. Consequently, a larger FA value change Δr (Δr, Δr = r1 − r2) was obtained and contributed to improve the sensitivity. In addition, the quantitative detection of the target DNA (T) was achieved according to the relationship between Δr and the concentration of T. In the presence of MIL-101, the Δr is 7-fold higher than that without MIL-101 and achieved the sensitive and selective detection of RSV DNA.
研究领域:
生物化学
·
分析化学
·
病毒学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Fan Jia,Dong Liu,Na Dong,Yuye Li,Shuyun Meng,Tianyan You
Signal amplification is one of the most effective ways to develop the high-performance electrochemical sensors . However, it can be more complicated for ratiometric detections. Herein, a ratiometric electrochemical aptasensor for aflatoxin B1 (AFB1) was proposed by taking advantage of a dual-amplification strategy by coupling of DNA walker (DW) with hybridization chain reaction (HCR). The special binding of AFB1 with ferrocene (Fc)-labelled aptamer triggers DW on hairpin DNA (hDNA) tracks to produce abundant double-stranded DNA (dsDNA). HCR-based strand amplification occurs on these dsDNA to absorb more methylene blue (MB). Then current ratio of MB (I MB ) and Fc (I Fc ) is designed as a yardstick to detect AFB1. Our experiments reveal that the interaction between Fc and MB (i.e., steric hindrance , electron mediator) varies. In addition to steric hindrance, the presence of MB also acts as electron mediator, thereby facilitating the electron transfer between Fc and electrode. Such combined effect consequently depresses the efficiency of dual-amplification strategy to improve the detection. The developed ratiometric electrochemical aptasensor allows the accurate detection of AFB1 in the 0.003–3 pg mL −1 range. Our work has shed light on the amplification strategy for ratiometric sensing, and provided a new route in integrating different amplification strategies.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Liang-Liang Wang,Wen-Qian Chen,Yu-Ru Wang,Lu-Peng Zeng,Ting-Ting Chen,Guan-Yu Chen,Jing-Hua Chen
Exosomal microRNAs (miRNAs) have been explored as an extremely promising biomarker of liquid biopsy for the diagnosis, treatment and prognosis of diseases such as cancer, in which sensitive and selective detection is significant. Herein, we describe the construction and testing of an electrochemical biosensor for the sensitive detection of exosomal miRNAs. It is based on synthetizing numerous long single-stranded DNAs (ssDNAs), which are produced by dual amplification reactions of target-triggered cyclic strand displacement reaction (TCSDR) and primer exchange DNA amplification reaction (PEDAR). In the first signal amplification step, target miRNAs are captured by the hairpin DNA strands (capture probes, Cp) that are immobilized on electrode. After strand unfolding with target capture, primer probes (Pp) enable to hybridize with Cp. And then target miRNAs were displaced for starting the TCSDR process that enable the introduction of numerous primers in Pp. In the second signal amplification step, the primers associated with PEDAR produce copious amounts of elongated ssDNAs. These ssDNAs absorb abundant quantities of methylene blue (MB) that enables the highly sensitive and label-free detection of exosomal miRNAs. This dual amplification process is characterized by a low limit of detection (LOD) of 3.04 aM. In addition, the electrochemical biosensor exhibits good selectivity for miR-21 detection, and shows benefits of simple operation, low cost, portability. Overall, the electrochemical biosensor provides a promising platform for the early diagnosis and screening of tumor biomarkers and the development of devices for point-of-care testing (POCT).
研究领域:
生物标志物检测
·
电化学
·
癌症诊断
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Lv Yuxiong,Sun Yingying,Liu Wenjie,Yu Jianna,Jing Guoxing,Liu Wen,Xia Huaiyue,Li Dongyan,Li Wenshan
Detection of ultralow concentration of tetracycline (TC) plays a key role in food safety and human health. Herein, we fabricated an enzyme-free dual-amplification assay for sensitive detection of TC in milk. The sensing system ingeniously combined Mg 2+ -dependent DNAzyme (MNAzyme) cleavage and catalytic hairpin assembly (CHA). Through the binding of TC and specific aptamer (Apt), DNA1 was released from the Apt-DNA1 complex. Then the separated DNA1 would hybridize with DNA2 to activate the catalytic activity of MNAzyme, which subsequently cleaved the substrate H0 and generated a new unit to trigger the following CHA reaction between H1 and H2, thereby releasing the G-rich sequence of H1 to induce the formation of G-quadruplex–hemin DNAzyme as a colorimetric signal readout. Under optimal conditions, the absorption intensity exhibited a significant linear correlation with the logarithm of the target TC concentration over a range of 4 orders of magnitude dynamic range from 1 pM to 10 nM. The limit of detection was calculated to be 0.89 pM and the method showed high selectivity for TC. More impressively, the proposed method was employed for TC determination in the milk sample.
研究领域:
分析化学
·
食品科学
·
生物技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2019
·
IF 6.944
Lin Shi,Yudie Sun,Lan Mi,Tao Li
Portable chemiluminescence (CL) imaging with a smartphone has shown a great promise for point-of-care testing of diseases, especially for acute myocardial infarction (AMI), which may occur abruptly. A challenge remains how to improve the imaging sensitivity that usually is several orders of magnitude lower than those of counterpart methodologies using the sophisticated equipment. Toward this goal, here, we report the target-triggered in situ growth of AuNP@hairpin-DNA nanoprobes into spherical nucleic acid enzymes (SNAzymes), which serve as both nanolabels and amplifiers for portable CL imaging of microRNAs (miRNAs) with an ultrahigh sensitivity comparable to that of the instrumental measurement under same conditions. A G-quadruplex (G4) DNA dense layer is dynamically produced on the gold nanocore via a DNAzyme machine-driven hairpin cleaving and captures the cofactor hemin to form the SNAzymes with higher peroxidase activity and stronger nuclease resistance than the commonly used G4 DNAzymes. The matured SNAzymes are then utilized as catalytic labels in a luminol-artesunate CL system for miRNA imaging with a smartphone as the portable detector. In this way, two AMI-related miRNAs, miRNA-499 and miRNA-133a, are successfully detected in real patients’ serum with a naked eye-visualized CL change at 10 fM, showing a 5 order of magnitude improvement on the sensitivity of visualizing the same disease markers in clinical circulating blood as compared to the reported strategy. In addition, a good selectivity of our developed CL imaging platform is demonstrated. These unique features make it promising to employ this portable imaging platform for clinical AMI diagnosis.
研究领域:
生物技术
·
生物化学
·
纳米技术
·
即时诊断
·
医疗器械
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）