Analytical Methods
·
2024
·Vol. 16
·期 16
·2472-2477
·
IF 2.7
Chenhao Zhao,Xingdan Wang,Jing Wu,Yeli Hu,Qiwei Zhang,Qi Zheng
Sialic acids are a family of monosaccharides that share a nine-carbon backbone and a carboxyl group. A recent derivatization method based on 3-nitrophenylhydrazine (3-NPH) provides a mild chemical labeling technique for biomolecules containing carbonyl or carboxyl groups. In this study, we utilized 3-NPH to label sialic acids via a two-step derivatization process. The derivatized species can produce a common reporter ion corresponding to C1–C3 with two labels, and a fragment differentiating between Neu5Ac, Neu5Gc, and KDN. This method is compatible with O-acetylated sialic acids and provides high sensitivity to Neu5Gc and KDN, and since the utilization of dual labeling significantly enhances the hydrophobicity of derivatives, it can effectively mitigate matrix effects when combined with parallel reaction monitoring technology. Negative-ion tandem mass spectrometry (MS/MS) analysis reveals a distinctive fragmentation profile for the 4-O-acetylated species, while the other sialic acids yield similar MS/MS spectra with a high abundance of reporter ions. Using the reporter ion as a transition, this analytical strategy is effective for analyzing complex biological samples. For example, it was successfully employed to quantify sialic acids in the intestinal tissues of several carp species, demonstrating its potential in sialylation research.
研究领域:
分析化学
·
糖生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2015
·Vol. 22
·期 19
·14882-14890
·
IF 2.828
Shi Haiyan,Sheng Enze,Feng Lu,Zhou Liangliang,Hua Xiude,Wang Minghua
A highly sensitive direct dual-labeled time-resolved fluoroimmunoassay (TRFIA) to detect parathion and imidacloprid simultaneously in food and environmental matrices was developed. Europium (Eu 3+ ) and samarium (Sm 3+ ) were used as fluorescent labels by coupling separately with L 1 -Ab and A 1 P 1 -Ab. Under optimal assay conditions, the half-maximal inhibition concentration (IC 50 ) and limit of detection (LOD, IC 10 ) were 10.87 and 0.025 μg/L for parathion and 7.08 and 0.028 μg/L for imidacloprid, respectively. The cross-reactivities (CR) were negligible except for methyl-parathion (42.4 %) and imidaclothiz (103.4 %). The average recoveries of imidacloprid ranged from 78.9 to 104.2 % in water, soil, rice, tomato, and Chinese cabbage with a relative standard deviation (RSD) of 2.4 to 11.6 %, and those of parathion were from 81.5 to 110.9 % with the RSD of 3.2 to 10.5 %. The results of TRFIA for the authentic samples were validated by comparison with gas chromatography (GC) analyses, and satisfactory correlations (parathion: R
2 = 0.9918; imidacloprid: R
2 = 0.9908) were obtained. The results indicate that the dual-labeled TRFIA is convenient and reliable to detect parathion and imidacloprid simultaneously in food and environmental matrices.
研究领域:
食品安全检测
·
环境监测
·
免疫学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Enze Sheng,Haiyan Shi,Liangliang Zhou,Xiude Hua,Lu Feng,Tong Yu,Minghua Wang
Europium (Eu 3+ ) and samarium (Sm 3+ ) were used as fluorescent labels to develop a highly sensitive dual-labeled time-resolved fluoroimmunoassay (TRFIA) for detect clothianidin and diniconazole in food samples. Under the optimized assay conditions, 50% inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of clothianidin were 5.08 and 0.021 μg/L, and 13.14 and 0.029 μg/L for diniconazole. The cross-reactivities (CRs) were negligible except dinotefuran (9.4%) and uniconazole (4.28%). The recoveries of clothianidin and diniconazole ranged from 79.3% to 108.7% in food samples. The results of TRFIA for the authentic samples were validated by gas chromatography (GC) analyses, and a satisfactory correlations were obtained. These results indicated that the method was an alternative tool for simultaneous detection of clothianidin and diniconazole in food samples.
研究领域:
分析化学
·
食品安全
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DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Tian Wei,Yongyi Zhang,Jianxin Huang,Jinyi Yang,Yuanxin Tian,Yudong Shen
In recent years, phosphodiesterase type 5 enzyme inhibitors, including tadalafil, sildenafil, and their analogues have emerged as illegal ingredients in dietary supplements. It can cause serious clinical side effects in consumers, resulting in diverse symptoms. To rapidly screen illegal drugs in dietary supplements, a dual-labeled probe time-resolved fluorescence immunochromatography assay (DL-TRFICA) based on two broad-spectrum antibodies labeled with time-resolved fluorescent microspheres was established for the simultaneous qualitative and quantitative detection of nine sildenafil-like and three tadalafil-like adulterants. The half-maximal inhibitory concentration (IC 50 ) of sildenafil-like and tadalafil-like adulterants were 2.49–5.88 and 2.15–2.78 ng mL −1 , the limit of detection were 0.08–1.66 and 0.59–1.85 ng mL −1 , and the visualization value (cut-off value) were 31.25–250 and 50–62.50 ng mL −1 , respectively. Notably, the potential practical applications of DL-TRFICA evaluated by conducting a quantitative evaluation of sildenafil and tadalafil adulterants in dietary supplement samples (herbal spirit drinks, healthcare oral liquids, and herbal medicine pills) demonstrated a recovery rate ranging from 80.0% to 125.8% with a coefficient of variation of less than 15%, which concluded that the DL-TRFICA approach could be used in real sample detection.
研究领域:
药物分析
·
分析化学
·
食品安全
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Rong Hu,Wei Wen,Qingling Wang,Huayu Xiong,Xiuhua Zhang,Haoshuang Gu,Shengfu Wang
A novel platform based on a hairpin oligonucleotide (HO) switch, gold nanoparticles (AuNPs), and enzyme signal amplification for the ultrasensitive detection of mucin 1 protein (MUC1) was developed in this assay. This HO aptamers and horseradish peroxidase (HRP) were immobilised on the AuNPs to yield HO–AuNP–HRP conjugates. AuNPs were used as labels and bridges between the HO and HRP. HRP was also used as label for catalysing the oxidation of o-phenylenediamine by H 2 O 2 . The reaction product was 2,3-diaminophenazine (DAP), which was reduced and could be detected at surface of modified electrode . The reduction signal of DAP was used as a probe for the sensitive detection . After the recognition between oligonucleotide and MUC1, biotin was exposed. Biotin, along with the conjugate , was captured by streptavidin onto the surface of modified electrode. Therefore, the detection of target MUC1 which was a membrane-associated glycoprotein of the mucin family could be sensitively transduced via detection of the electrochemical reduction signal of DAP. Compared to other aptasensors , this biosensor has a good linear correlation ranges from 8.8 nM to 353.3 nM and a lower detection limit of 2.2 nM for MUC1. The proposed method provided a new electrochemical approach for the detection of MUC1.
研究领域:
生物传感器
·
分析化学
·
癌症诊断
·
纳米技术
·
生物化学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Liangrui Lv,Wei Yuan,Juanjuan Hu,Xuyuan Chen,Tingting Zhang,Jie Cheng,Xiaoying Wang
Protein tumor marker (PTM) analysis is of great significance for the early diagnosis and process monitoring of tumors. However, the clinical reference value of a single PTM is restricted, and existing tests for multiple PTMs majorly suffer from complex processes and poor sensitivity. Herein, a cancerous inhibitor of protein phosphatase 2A (CIP2A) and protein c-Myc (AGc-Myc) with a positive feedback path in oral squamous cell carcinoma (OSCC) was chosen as a dual PTM, and a magnetic electrochemical immunosensor (MEI) for their simultaneous detection was successfully established. Fe3O4 coated with a gold nanoparticle (MAu)-functionalized magnetic electrode (f-MGE) was of benefit to immobilize two capture antibodies (CAb) through internal forces and external magnetic fields. By a mechanical alloying (MA) method, two photoelectric materials osmosed bimetallic-matrix nanospheres (Bi-MPE), including luminol and Ru(bpy)32+ osmosed AuNPs–AgNPs nanoalloy (Lu@AuAg and Ru@AuAg), were prepared as innovative signal probes. Lu@AuAg and Ru@AuAg not only exhibit superior electrochemical properties than photoelectric material osmosed monometallic-matrix nanospheres but can directly label detection antibodies (DAb) without complex reaction conditions and additional functionalization. After immunological reactions, the distinguishable differential voltammetry pulse signals were obtained at +0.48 and +1.13 V, corresponding to AGc-Myc and CIP2A, respectively. The proposed assay showed a good linear response range from 5 pg L–1 to 5 ng L–1 for AGc-Myc and 50 pg L–1 to 50 ng L–1 for CIP2A, and a low limit of detection of 2.5 pg L–1 and 25 pg mL–1, respectively. The MEI also exhibited satisfactory selectivity, stability, and reproducibility. Moreover, when the proposed method and the ELISA kit were simultaneously applied to saliva samples of OSCC patients, the obtained results were completely consistent, reflecting the clinical practicability of this work.
研究领域:
生物化学
·
生物技术
·
分析化学
·
医学诊断
·
纳米技术
·
电化学
·
癌症研究
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）