Cancer Medicine
·
2024
·Vol. 13
·期 17
·e70197
·
IF 2.9
Yidan Ma,Yifei Wang,Lei He,Jun Du,Lin Li,Zhixin Bie,Yuanming Li,Xiaomao Xu,Wei Zhou,Xiaonan Wu,Li Yang,Jing Di,Chenyang Li,Xiaoguang Li,Dongge Liu,Zheng Wang
Backgroud Supernatants from various cytological samples, including body cavity effusion, sputum, bronchoalveolar lavage fluid (BALF), and needle aspiration, have been validated for detecting genetic alterations using cell-free DNA (cfDNA) in patients with non-small cell lung cancer (NSCLC). However, the sensitivity of fusion variations detection remains challenging. The protection of cell-free RNA (cfRNA) is critical for resolving the issue. Methods A protective solution (PS) was applied for preserving cfRNA in cytological supernatant (CS), and the quality of protected cfRNA was assessed by cycle threshold (CT) values from reverse transcription quantitative polymerase chain reaction (RT-qPCR). Furthermore, we collected an additional set of malignant cytological and matched tumor samples from 84 NSCLC patients, cfDNA & cfRNA extraction and double detection for driver gene mutations was validated using the multi-gene mutations detection by RT-qPCR. Results Under the optimal protection system, 91.0% (101/111) of cfRNA were protected effectively. Among the 84 NSCLC patient samples, seven cytological samples failed the tests. In comparison with tumor samples, the overall sensitivity and specificity of detecting driver genes of supernatant cfDNA and cfRNA were 93.8% (74/77) and 100% (77/77), respectively. Notably, when focusing exclusively on patients with fusion gene changes, both sensitivity and specificity reached 100% (11/11) for EML4-ALK, ROS1, RET fusions, and MET ex14 skipping. Conclusion These findings suggest that cfDNA & cfRNA extraction and double detection strategy recommended in this study improve the accuracy of driver genes mutations test, especially for RNA-based assay.
研究领域:
医学
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分子生物学
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DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Zixuan Shang,Pengfei Ma,Imran Mahmood Khan,Ningru Yang,Zhouping Wang
Currently, the technology for detecting antibiotics is developing rapidly, with good sensitivity and selectivity. However, some detection methods still have issues with cumbersome pretreatment and high experimental costs. Herein, A “turn-off” bifunctional DNA tweezer was constructed through the design of DNA sequence and self-assembly technology for the dual detection of Enrofloxacin (ENR) and Kanamycin (KANA) in a food matrix. The operation is simple, which increases the detection speed and reduces the detection cost. The two ends of the bifunctional DNA tweezers are connected with specific split aptamer sequences, and the split aptamer forms a specific ternary complex with the target. The tweezers “clip” ENR and KANA at their respective ends, causing a structural change. Under the optimal experimental conditions, the linear range of detection ENR is 0.2 ng/mL-1000 ng/mL with an LOD of 0.18 ng/mL, the linear range of detection KANA is 0.5 ng/mL-1000 ng/mL with an LOD of 0.36 ng/mL. The bifunctional DNA tweezers involved provide a new idea for the construction of DNA nanomachines, which is also suitable for the recognition and detection of other small molecules.
研究领域:
生物化学
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食品科学
·
纳米技术
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Bangjie Yin,Zheng Zhang,Yingchun Wang,Hui Zeng,Jin Xu,Hongzhou Li,Yan Li,Min Zhang
The growing demand for tailored detectors in capillary electrophoresis (CE), addressing tasks like field deployment or dual-detection analysis, emphasizes the necessity for compact detection cells. In this work, we propose cost-effective and user-friendly additive manufacturing (3D-printing) approaches to produce such miniaturized detection cells suitable for a range of CE applications. Firstly, capacitively-coupled contactless conductivity detection (C 4 D) cells of different sizes are fabricated by casting low-melting-point alloy into 3D-printed molds. Various designs of Faraday shields are integrated within the cells and compared. A mini-C 4 D cell (9.5×7.0×7.5 mm 3 ) is produced, with limits of detection for alkaline cations ranging from 8-12 μM in a short–capillary based CE application. Secondly, ultraviolet photometric (UV-PD) detection cells are fabricated using 3D printing. These cells feature two narrow slits with a width of 60 μm, which are positioned along the path of incident and transmission light to facilitate collimation. A deep UV-LED (235 nm or 255 nm) is employed as the light source, and black resin is determined to be the optimal material for 3D printing the UV-PD cell, owing to its superior UV light absorption capabilities. The UV-PD cell is connected to the LED and photodetector through two optical fibers, making it easy to switch the light source and detector. The effective pathlength and stray light percentage for detecting on a 75 μm id capillary are 74 μm and 0.5 %, respectively. Thirdly, a dual-detection cell that combined C 4 D and UV-PD at a single detection point is proposed. The performance of direct detection by C 4 D and indirect detection by UV-PD is compared for detecting organic acids. The strategies for developing cost-effective compact detection cells facilitate the versatile integration of multiple detection methods in CE analysis.
研究领域:
分析化学
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生物分析
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仪器科学与技术
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yahang Li,Hanrui Zhou,Qing Song,Mingzhu Zou,Ying Wei,Qingguo Zhang
The emergence of flexible, painless sensors for biomolecule measurement represents a growing trend in biomedical research. In this context, a highly sensitive flexible sweat sensor, incorporating MnO 2 -TMB@PAM aggregates employing dual-detection routes via visualization and UV–vis spectrometry, has been developed. The sensor utilizes bovine serum albumin (BSA) templated MnO 2 nanoparticles (NPs) as a catalyst for glucose detection. Unlike natural enzymes, this nanozyme is not only cost-effective and stable but also exhibits tandem enzyme-like activities, which facilitates quick and straightforward glucose detection. MnO 2 NPs are synthesized in situ on the surface of polyacrylamide (PAM), ensuring high stability and increasing the contact area of reactors, thereby enhancing the catalytic efficiency of MnO 2 NPs . Additionally, 3,3′,5,5′-tetramethylbenzidine (TMB) is modified on the hydrogel to create an integrated sensing patch (referred to as MnO 2 -TMB@PAM). This patch enables fast and convenient sensing routes, positioning it as a potential candidate for point-of-care testing (POCT) devices. Due to the excellent flexibility and transmittance of PAM , the sensing patch allows for rapid colorimetric analysis of biomolecules such as glucose and dopamine via UV–vis spectrophotometry, as well as visual detection with the naked eyes. Consequently, a high-sensitive, selective, and visually active flexible sensor has been established based on colorimetric and visual methods, facilitating efficient sensing of biomarkers from in vitro biological fluids.
研究领域:
生物医学工程
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材料科学
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分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaozhou Li,Tianyue Yang,Youtao Song,Jinghai Zhu,Deli Wang,Wei Li
Here we describe a surface-enhanced Raman scattering (SERS)-based immunochromatographic assay (ICA) method for the dual detection of two pyrethroid pesticides cypermethrin and esfenvalerate. In the proposed ICA-SERS methods, antibody conjugated gold nanoparticles (AuNPs) were used as the SERS substrate while fluorescence tags labeled on AuNPs were used as the Raman reporters for SERS. Through the immuno-specific combination of antigen and antibody, Raman reporter labeled AuNPs were immobilized on the test line of the ICA strip and facilitated the SERS measurement. And by immobilizing two test lines designed for the detection of two pesticides, a simultaneous dual detection was accomplished. The LODs of the ICA-SERS system was 2.3 × 10 −4 and 2.6 × 10 -5 ng/mL for cypermethrin and esfenvalerate, respectively. The sensitivity was 3 to 4 times higher than ELISA and fluorescent-based ICA methods using the same indirect competitive immunoassay methods. For the three types of samples (tap water, river water, and milk), the recoveries ranged from 94.9% to 112%, with RSD values ranged from 1.3% to 13.5%. The research reveals that this ICA-SERS method possesses high specificity, sensitivity, and reproducibility, and can be reliably used to detect dual pesticides in real samples. An R Shiny-based application 2plex-speclysis was built and used for all the data analyses in this paper. The application was designed to incorporate all the analyses including spectral preprocessing, performance evaluation, and PCA analysis, and make the spectral analysis accessible for people without programming experience.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Tingting Lei,Yating Xu,Yuanyu Tang,Siyi Tan,Jiafu Xiao,Shaojing Zhao,Minhuan Lan
Achieving highly selective visual detection of thiram is essential for ensuring food safety and environmental protection. Here, we developed a smartphone-integrated colorimetric and fluorometric dual-mode sensor based on copper-doped carbon dots (Cu-CDs). The Cu-CDs were synthesized via hydrothermal treatment of L-tryptophan and CuCl2·2H2O. These Cu-CDs exhibited two fluorescence emission peaks at 350 nm and 450 nm, with a fluorescence quantum yield of 20.8%. Cu2+ on the surface of the Cu-CDs could selectively bind to thiram to form a Cu2+-thiram complex, which quenched the fluorescence at 450 nm of Cu-CDs via the inner filter effect and dynamic quenching effect. In contrast, the fluorescence at 350 nm was changed slightly. The color of the solution was changed from colorless to yellow. These changes allowed for a fluorometric and colorimetric dual-mode optical response. The proposed sensor possessed high sensitivity and selectivity and demonstrated reliable performance in real water samples. Its integration with a smartphone further enabled convenient, on-site detection. This work presented a robust and cost-effective strategy for monitoring thiram in applications related to food safety and environmental protection.
研究领域:
分析化学
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食品安全
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环境监测
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纳米生物技术
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传感器开发
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2020
·Vol. 22
·期 1
·1-13
·
IF 2.132
Zhou Xin,Qu Qing,Wang Lin,Li Lei,Li Shunling,Xia Ke
A novel amine-modified carbon quantum dots (NH 2 -CQDs), which can act as a dual-detection probe for the ascorbic acid (AA) response, was synthesized. The product was obtained via facile and green hydrothermal carbonization of L-histidine. The high nitrogen to carbon ratio of L-histidine warrants a high nitrogen content of these CQDs, and thus to have more free electron pairs. This results in good electron transport capability. The NH 2 -CQDs with an average diameter of 3.67 ± 0.78 nm display blue fluorescence, with excitation/emission maxima at 340/420 nm, and a quantum yield of 15.9%. The fluorescence can be quenched by AA. Moreover, the NH 2 -CQDs can selectively detect AA with electrochemical detection. On the basis of the electrochemical and fluorescent properties of the NH 2 -CQDs, the electrochemistry method and fluorescence analysis for the detection of AA with a detection limit of 2.7 μM and 57 nM are developed. This finding may provide a dual-detection platform, which can be used as a potential candidate for more selective to detect AA in pharmaceuticals, clinics, and food industries.
Schematic representation of the synthesis strategy of amine-modified carbon quantum dots (NH 2 -CQDs) and their electrochemical and fluorescent response to ascorbic acid (AA).
研究领域:
材料科学
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分析化学
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纳米技术
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Xinyi Liu,Dilong Hong,Di Zhang,Yuliang Jiang
Tetracycline and norfloxacin are two important antibiotics known for their effective treatment of inflammation. However, their excessive use inevitably leads to serious environmental hazards. Rapid and highly sensitive detection of their trace amounts is crucial for early prevention of related diseases. While there are numerous reported fluorescent probes capable of highly sensitive detection of tetracycline and norfloxacin individually, very few can simultaneously detect both. Developing a probe capable of detecting both antibiotics simultaneously is particularly necessary to enhance detection efficiency. In this study, carbon quantum dots derived from fragrans were functionalized with Metal-Organic Framework (MOF) materials. This successful approach enabled simultaneous detection of both tetracycline and norfloxacin, with detection limits of 0.15 μM and 0.87 μM, respectively. Furthermore, successful detections were achieved in water, milk and soil samples, yielding excellent results.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Jinxiu Hou,Yi Xu,Shasha Sun,Xiaoli Zhong,Chih-Tsung Yang,Xin Zhou
Label-free SPR is rarely used for biomarker detection in real samples due to its low sensitivity. Herein, we report a phage-based SPR (P-SPR) strategy for the detection of carcinoembryonic antigen (CEA) in undiluted serums. M13 phage is genetically engineered to display an anti-CEA single-chain variable region fragment on pIII protein for antigen targeting and to display the gold binding peptide (GBP) at the pVIII protein for gold nanoparticles (GNPs) binding to form the GNPs decorated M13 phage probe that can amplify the plasmon resonance signal. The proposed P-SPR method demonstrated ultrasensitive detection of CEA within 10 min. Under ideal conditions, P-SPR showed exceptional sensitivity with a limit of detection (LOD) of 0.83 fM, which is approximately 2000 times that of the conventional SPR and four orders of magnitude more sensitive than enzyme-linked immunosorbent assay. In particular, the proposed strategy can cross-validate the SPR results through naked-eye counting of the plaques generated by the P-SPR probes, enabling a reliable dual detection system. In addition, P-SPR scheme has excellent sensing performance for the detection of real samples at concentrations lower than LOD of ELISA. Therefore, the proposed P-SPR strategy holds potential for early detection of tumour biomarkers in liquid biopsy.
研究领域:
生物传感器
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纳米技术
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癌症诊断
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分析化学
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分子生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Qingyan Han,Hao Zhang,Andrey Kaplan,Shixing Fan,Yazhou Xu,Chengyun Zhang,Wei Gao,Enjie He,Jun Dong
Surface-enhanced Raman scattering (SERS) with ultrahigh sensitivity has garnered significant attention for quantitative analysis and chemically specific detection. However, conventional SERS platforms, typically structured by depositing plasmonic micro/nanoparticles onto rigid substrates, face limitations in further advancement and applications. In this study, we present a novel method to prepare a flexible SERS film substrate composed of graphene overlayer (G) atop a self-assembled array of silver micropopcorns (Ag MPs) and a polymethyl methacrylate (PMMA) membrane. We show that adding an overlayer of graphene improves substantially overall SERS performance. This flexible G/Ag MPs/PMMA film substrate achieves a detection limit as low as 100 picomolar for rhodamine 6G (Rh6G) molecules and identifies mixtures containing Rh6G (10 −11 M) and crystal violet (CV, 10 −6 M) molecules. Furthermore, the flexible SERS structure enables quantitative detection of thiram, achieving a detection limit of 0.005 ppm, well below the allowable limit in food. This study provides a robust SERS platform that advances fundamental research and facilitates the development of practical applications in quantitative analysis, detection, and biological sensing. Combining graphene and Ag MPs on a flexible PMMA substrate offers a versatile and scalable approach to enhancing SERS performance, paving the way for future innovations.
研究领域:
分析化学
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纳米技术
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材料科学
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光学传感
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化学检测
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Changjin Zhou,Jiahao Zhang,Hongyi Li,Dong Shao,Junlun Zhu,Wei Wen,Xiuhua Zhang,Zhengfang Tian,Shengfu Wang
As a key technology enabling green and sustainable chemical processes, nanozymes have significantly enhanced their catalytic efficiency and economic viability. Therefore, the development of a higher catalytic activity enzyme mimic to construct a portable and easily sensing strategy for sensitive, rapid, and convenient determination of a specific target is exceedingly elemental for an advanced sensing platform. Herein, we designed and prepared copper-doped palladium metallene (Cu–Pd metallene) with multiple enzyme-like activities for a sensitive colorimetric assay. The relevant kinetic parameters of Cu–Pd metallene with three kinds of enzyme-like activities were evaluated. The Michaelis–Menten constant of laccase mimic and oxidase mimic were calculated to be 0.041 and 0.51 mM, which exhibited high affinity to 2,4-dichlorophenol and 3,3′,5,5′-tetramethylbenzidine in the corresponding system, respectively. Inspired by its high catalytic activity, a simple and portable colorimetric sensing platform based on Cu–Pd metallene was developed to detect and distinguish epinephrine, phenolics, and thiram by UV–vis absorption spectrum and smartphone. Among them, the proposed method can detect epinephrine concentration in the range of 1–15 μg mL–1 by laccase-like activity, and a calculated reliable limit of detection is 0.18 μg mL–1. Owing to the inhibitory effect, the colorimetric sensing platform was used to analyze thiram, which presented a concentration range of 10–500 ng mL–1 and an acceptable detection limit of 4.4 ng mL–1. Considering the advantages of accuracy and ease of use of Cu–Pd metallene-based colorimetric sensing platform, it has great potential for real-time monitoring of relevant targets in poor areas.
研究领域:
纳米技术
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分析化学
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生物传感器
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酶模拟
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环境监测
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食品安全
·
即时检测
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）