Analytical Methods
·
2021
·Vol. 13
·期 40
·4747-4755
·
IF 2.896
Wendi Lv,Xiaoying Yuan,Chenglu Yan,Qiuting Ma,Bing Wang,Juan Du,Baozhan Zheng,Dan Xiao
Eu3+-Doped nanoceria (Eu:CeO2) with self-integrated catalytic and luminescence sensing functions was synthesized by a simple and gentle one-pot method to build a dual-readout nanozyme platform for organophosphate compound (OPC) sensing in this work. The catalytic degradation of the model substrate of OPC, p-nitrophenyl phosphate (p-NPP), by as-prepared Eu:CeO2 can be completed in 2 min with little influence of temperature and pH values, highlighting the advantages of Eu:CeO2 as an artificial enzyme for dephosphorylation. Most importantly, the characteristic red emission of Eu3+ (592 nm) from Eu:CeO2 can be quenched by p-NPP, accompanied by a color change from colorless to yellow. Based on this, linear ranges of 4–50 μM with a detection limit of 3.3 μM and 1–20 μM with a detection limit of 0.6 μM for p-NPP were obtained by colorimetric and fluorescence methods, respectively. Furthermore, the fluorescence strategy was effectively applied to the determination of ethyl para-nitrophenyl (EPN), one of the most commonly used pesticides, with a detection limit of 5.86 μM. The proposed strategy was also successfully applied to the assay of p-NPP and EPN in real water samples, showing great application prospects in detecting OPC in the environment.
研究领域:
生物化学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 38
·6099-6107
·
IF 4.776
Yifang Gao,Yuan Jiao,Wenjing Lu,Yang Liu,Hui Han,Xiaojuan Gong,Ming Xian,Shaomin Shuang,Chuan Dong
A novel visual probe with red fluorescent carbon dots (CDs) has been facilely fabricated for the sequential detection of chromium(VI) [Cr(VI)] and cysteine (Cys) via a fluorescent and colorimetric double-mode readout assay. The as-prepared CDs exhibit not only exceedingly optical stability, but also excellent biocompatibility and biolabeling potentials. The fluorescence of the CDs can be extinguished dramatically with Cr(VI). After the addition of Cys, the fluorescence of the CDs was restored due to the complexation between Cr(VI) and Cys that leads Cr(VI) to escape from the surface of CDs. The probe displayed high selectivity and sensitivity toward Cr(VI) and Cys over other analytes with a low detection limit of 0.26 μM and 0.14 μM, respectively. Simultaneously, the color of the CDs solution was changed (red–purple–yellow) along with the fluorescence signal of the CDs, and the CDs can be used as a dual response signal sensor. Based on the above results, an “AND” logic gate for red-emitting CDs has been constructed. Moreover, to the best of our knowledge, it is the first red-emissive carbon dots for chromium(VI) and cysteine assays. The probe was extended to cellular imaging. Based on the simplicity, time-saving, and cost-effectiveness of the constructed program, this probe shows great potential in biosensing, bioimaging, and environmental monitoring.
研究领域:
分析化学
·
生物传感
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Jiahui Zhao,Xingfu Bao,Shuang Wang,Shasha Lu,Jian Sun,Xiurong Yang
As a well-known copper-containing oxidase, tyrosinase has been anticipated to serve as the biomarker of skin diseases. We describe here an exquisite label-free fluorescent and colorimetric dual-readout assay of its activity, inspired by the specific oxidation ability of monophenolamine substrates to catecholamines and a unique fluorogenic reaction between resorcinol and catecholamines. By employing commercially available tyramine as the model substrate (dopamine as the product), it is found that the tyrosinase-incubated tyramine solution exhibits obvious pale yellow with intense blue fluorescence in the presence of resorcinol and O2, where the absorbance and fluorescence intensity are directly related to the concentration of added tyrosinase (i.e., the amount of conversion of tyramine to dopamine). The overall process of sensing tyrosinase activity takes less than 100 min at ambient temperature and pressure conditions with exceedingly simple operation procedure, explicit response mechanism, and formation of fluorophore with high quantum yield from scratch. Furthermore, such a convenient, rapid, cost-effective, and highly sensitive dual-readout assay exhibits promising prospect for the tyrosinase activity in extensive bioassays and clinic research as well as in screening potential tyrosinase inhibitors.
研究领域:
生物化学
·
医学诊断
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Liu Jiang,Yuhao Wu,Haichuan Li,Jiayi Sun,Xiaolong Wang,Yuankui Leng,Liang Guo,Xiaolin Huang,Yonghua Xiong
Background Traditional immunochromatographic assay (ICA) test strips typically utilize colloidal gold nanoparticles as colorimetric signal reporters, but they often suffer from compromised sensitivity and quantitative accuracy, making them inadequate for the stringent demands of trace-level screening. In addition, the heterogeneous nature of food matrices, such as dairy products, meat, and ready-to-eat fruits and vegetables, complicates the accuracy and reliability of ICA for detecting foodborne pathogens. Therefore, enhancing the detection accuracy of ICA is critical to meet the stringent requirements for pathogen detection in complex food samples. Results Here, we synthesized a novel Prussian blue-coated Fe 3 O 4 nanoparticle (Fe 3 O 4 @PB) via a simple in-situ growth method. The synthesized Fe 3 O 4 @PB nanoparticles demonstrate a high magnetization strength and excellent photothermal conversion efficiency, enabling their application as efficient probes in ICA. Leveraging these properties, we developed a dual-readout ICA incorporating both colorimetric and photothermal modalities for the sensitive detection of Salmonella typhimurium in milk and lettuce samples. The detection limit (LOD) for Fe 3 O 4 @PB-ICA reached 2.5 × 10 3 CFU/mL in colorimetric formats and 1.04 × 10 3 CFU/mL in photothermal formats, representing approximately 4-fold and 10-fold improvements compared to AuNP-ICA (LOD = 1 × 10 4 CFU/mL). Furthermore, the average recovery rates ranged from 89.1 % to 111.9 %, while the coefficients of variation below 13.7 %, demonstrating excellent accuracy and precision. Significance The proposed Fe 3 O 4 @PB serves as effective probe for purifying and enriching target analytes, significantly improving the accuracy and sensitivity of colorimetric and photothermal immunochromatographic analysis applications. By leveraging the magnetic and photothermal capabilities of Fe 3 O 4 @PB, the developed Fe 3 O 4 @PB-ICA represents a promising rapid diagnostic platform for the sensitive detection of foodborne pathogens and other analytes.
研究领域:
食品安全检测
·
纳米材料
·
微生物学
·
免疫分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Shuai Zhang,Jincheng Xiong,Boyan Sun,Chunchao Yin,Yongjun Zheng,Xingxing Liu,Yanchen Gao,Xiaomei Wang,Sihan Wang,Haiyang Jiang
Emerging contaminants seriously jeopardize food safety and even human health. This study presents the formation of all-in-one aggregation-induced emission (AlE)-type immunomagnetic ZlF-8 (AlMZ) through an ultra-simple self-assembly method in an aqueous phase, successfully harnessing the functions of immuno-recognition, luminescence, and magnetism, Here, we have achieved a dual enhancement in detection efficiency rapidly enriching targets and performing a triple lateral flow immunoassay (LFlA) for ractopamine, diethylstilbestrol, and chloramphenicol within 40 minutes, The dual readout biosensor was then configured to automatically extract the color intensity values of the three test lines using a smartphone. A portable sensing platform ensures stable temperature and light intensity in non-laboratory environments, providing favorable conditions for immunological detection. This study demonstrated low detection limits of 0.11, 0.14, and 0.01ug/L for ractopamine, diethylstilbestrol, and chloramphenicol, respectively, Applications in pork and tap water showed excellent practicality and stability, with recovery rates ranging from 87.2 % to 112.7 %, emphasizing the potential for applications in food and environmental safety.
研究领域:
食品安全检测
·
环境监测
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Dandan Hu,Tianyu Zhan,Zhiyong Guo,Sui Wang,Yufang Hu
A novel dual-modality readout biosensing method is developed for Cu(II), coenzyme (CoA) and histone acetyltransferase (HAT) analysis based on an electrosynthesized tris(bipyridine)ruthenium(II)-functionalized metal-organic framework (Ru-MOF). In this protocol, the Ru-MOF with a delicate structure serves as both an electrochemiluminescent (ECL) and fluorescent (FL) probe. Interestingly, Cu(II) can hinder the two distinct processes favorably, thus realizing bidirectional detection of Cu(II) via an “on-off” method. Simultaneously, CoA, which contains a sulfhydryl group, can ignite a reverse “off-on” process due to the polymeric interaction of CoA and Cu(II) (2: 1). Similarly, abundant CoA molecules could also be generated regularly by a typical HAT p300-driven acetylation reaction, hence accomplishing HAT p300 activity analysis. Subsequently, an ingenious “on-off” route is exhibited again by the introduction of some small molecules (including anacardic acid and C646), which present an inhibiting effect on HAT p300 activity. Furthermore, quantitative analysis of Cu(II), CoA and HAT p300 (ECL/FL) is achieved brightly with detection limits of 0.19/0.76 nM, 1.3/6.5 nM and 1.5/8.8 nM, respectively. Ultimately, this two-channel biosensor with excellent analytical performance is applied successfully in real samples, demonstrating its potential use in bioanalysis.
研究领域:
分析化学
·
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Jie Wei,Huaming Chen,Haihang Chen,Yunyan Cui,Aori Qileng,Weiwei Qin,Weipeng Liu,Yingju Liu
In this study, a versatile dual-modal readout immunoassay platform was achieved for sensitive and broad-spectrum detection of ochratoxins based on the photocurrent response of flexible CdS/ZnO nanorod arrays/reduced graphene oxide and the localized surface plasmon resonance (LSPR) peak shift of Au nanobipyramids (Au NBPs). By using nanoliposomes as the vehicle to carry the secondary antibody and encapsulate horseradish peroxidase (HRP), the photocurrent change and the peak shift can be greatly amplified. The reaction mechanism was investigated in detail, indicating that HRP can trigger enzymatic bioetching in the presence of H2O2. In the photoelectrochemical detection, the oxidized HRP can etch CdS on the photoelectrode, resulting in the photocurrent change, while in the colorimetric detection, HRP can oxidize H2O2 to produce hydroxyl radicals that can etch Au NBPs to form multiple color changes and LSPR shifts. Compared with the common single-modal immunoassay for ochratoxins, such dual-modal immunoassay is more precise and reliable, owing to the completely independent signal conversion and transmission mechanism. Therefore, we hope that this accurate, simple, and visualized strategy may create a new avenue and provide innovative inspiration for food analysis.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）