ANALYTICAL CHEMISTRY
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2023
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IF 7.4
Yin Zhao,Dongjuan Chen,Zhichen Xu,Tao Li,Jiang Zhu,Rui Hu,Guoyong Xu,Ying Li,Yunhuang Yang,Maili Liu
Fast, simplified, and multiplexed detection of human papillomaviruses (HPVs) is of great importance for both clinical management and population screening. However, current HPV detection methods often require sophisticated instruments and laborious procedures to detect multiple targets. In this work, we developed a simple microfluidic dual-droplet device (M-D3) for the simultaneous detection of HPV16 and HPV18 by combining the CRISPR-Cas12a system and multiplexed recombinase polymerase amplification (RPA) assay. A new approach of combining pressure/vacuum was proposed for efficient droplet generation with minimal sample consumption. Two groups of droplets that separately encapsulate the relevant Cas12a/crRNA and the fluorescent green or red reporters are parallelly generated, followed by automatic imaging to discriminate the HPV subtypes based on the specific fluorescence of the droplets. The M-D3 platform performs with high sensitivity (∼0.02 nM for unamplified plasmids) and specificity in detecting HPV16 and HPV18 DNA. By combining the RPA and Cas12a assay, M-D3 allows on-chip detection of HPV16 and HPV18 DNA simultaneously within 30 min, reaching a detection limit of 10–18 M (∼1 copy/reaction). Moreover, the outstanding performance of M-D3 was validated in testing 20 clinical patient samples with HPV infection risk, showing a sensitivity of 92.3% and a specificity of 100%. By integrating the dual-droplet generator, CRISPR-Cas12a, and multiplexed RPA, the M-D3 platform provides an efficient way to discriminate the two most harmful HPV subtypes and holds great potential in the applications of multiplexed nucleic acid testing.
研究领域:
分子生物学
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医学检测
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
THERMOCHIMICA ACTA
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2021
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IF 3.115
Baozhong Zhu,Weiqi Chen,Yunlan Sun,Peng Guo,Jianzhong Liu
Nanofluid fuels can be used in scramjet . However, the interactions among fuel droplets in spray and combustion process are still a gray area. The study on the evaporation and combustion of the double droplets can better demonstrate the role of nanofluid droplets. In this paper, the evaporation and combustion characteristics of the double droplets of aluminum/n-heptane based nanofluid fuels were investigated by a droplet-suspension method, whose results were compared to those of the single droplet at 973 K and 0.1 MPa. The effect of the distance of the double droplets on their evaporation behavior, ignition delay time, combustion phenomena, and micro-explosion time were studied. The influence of thermocouple wire was also analyzed. The double droplets have a synergistic effect on the evaporation behavior, ignition delay time, and micro-explosion. The ignition delay time of double droplets of the aluminum/n-heptane based nanofluid fuel droplet has debased 25.3% compared with that of the single droplet, while the maximum micro-explosion duration has increased 7.2%. With the increase of the distance of both droplets, the synergistic effect is first strengthened and then weakened.
研究领域:
能源工程
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燃烧科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
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IF 5.518
Meifang Liu,Qiang Chen,Jie Li,Yiyang Liu,Xiaoyu Yang,Fanghua Zhu,Zhibing He
Driven by the need to prepare stable double droplets (the precursor of polymer shells for the laser-driven inertial confined fusion (ICF)) with surfactant concentration as little as possible, a synergistic noncovalent interaction route of the hydrophilic and hydrophobic interactions, hydrogen bonding and electrostatic repulsion to improve the oil-water interface performance has been proposed. Both the adsorption layer thickness and the adsorbed amount of the surfactants on the oil-water interface increase due to the formation of the hydrogen bonding between the poly(vinyl alcohol) (PVA) and polyelectrolytes (polystyrene sulfonate sodium (PSSS) or poly(acrylic acid) sodium salt (PAAS)), leading to the improvement of the interface viscoelasticity and the decrease of the interfacial tension compared with that of the polyelectrolytes alone. Therefore, owing to the synergistic noncovalent interactions, the stability of millimeter-scale double droplets is improved and monodisperse polymer shells have been successfully prepared from these stable double droplets by solvent evaporation method. This work provides more in-depth insights of the synergistic effects of the surfactants on the double droplets stability and gives some guidance on the optimization of the surfactants, benefiting the preparation of monodisperse polymer shells for ICF.
研究领域:
材料科学
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能源科学
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物理化学
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2019
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IF 3.375
Yongli Sun,Xiang Gao,Na Yang,Xiaowei Tantai,Xiaoming Xiao,Bin Jiang,Luhong Zhang
With the aim of simultaneously achieving synthesis and morphology control of three-dimensional (3D) hierarchical layered double hydroxide (LDH) by more efficient and environmentally friendly methods, a facile and cost-efficient double-drop coprecipitation method was creatively applied to fabricate 3D flowerlike microstructures (Al2O3-MgAl-LDHs) using γ-Al2O3 as a sacrificial template. The morphology evolution process and growth characteristics of Al2O3-MgAl-LDHs were investigated in detail, revealing that γ-Al2O3 not only provides an effective support for the crystal growth of LDH but also is involved in the LDH layer formation process through self-decomposition. More importantly, LDH morphologies can be regulated by varying the reaction conditions, such as the γ-Al2O3 concentration, Mg2+/Al3+ molar ratio, and pH. Additionally, a possible formation mechanism for 3D hierarchical flowerlike Al2O3-MgAl-LDHs was illustrated. Remarkably, owing to the high specific surface area and low/medium/high-strength basic sites, the as-obtained LDH after calcination exhibited excellent catalytic activity and reusability in the transesterification of glycerol with dimethyl carbonate.
研究领域:
材料科学
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催化
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化学工程
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Energetic Materials
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2024
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IF 1.7
Ying Wu,Yong Liu,Shuai Hou,Qizhi Zhao,Han Gao,Xiao Yan
4,4’-Azo-1,2,4-triazole (atrz) is a new high nitrogen energetic material with high energy density, high gas yield and wide application prospects, but the low synthesis temperature and long time are the main reasons limiting its application. To achieve efficient synthesis of atrz, the double drop synthesis method was designed to solve the problem of unstable concentration of oxidant (HClO) due to batch feeding. The reaction temperature, reaction time, and molar ratio of reactants of n(4-amino-1,2,4-triazole):n(ClO−) were studied and optimized using the single factor variable method; Characterization and analysis of atrz using theoretical calculations with elemental analysis, infrared spectroscopy, crystal morphology, and nuclear magnetic resonance; The thermal properties and stability of atrz were tested and analyzed using TG-DTA. The results showed that the reaction time of the double drop synthesis method was shortened from 5 hours to 1.5 hours under the conditions of similar yield, and the efficiency was increased by 2.3 times. The optimized process was a reaction temperature of 5°C-10°C, reaction time of 1.5 hours, n(4-amino-1,2,4-triazole):n(ClO−) of 1:2, and the yield reached 73%. The product was white, needle-like crystals, which were determined to be atrz by means of characterization and theoretical calculations. Atrz’s decomposition peak temperature is 309.0°C, and when the temperature is below 271.7°C, atrz is thermally stable. The activation energies of atrz calculated by Kissinger and Flynn-Wall-Ozawa methods were 195.0 kJ·mol−1 and 194.6 kJ·mol−1.
研究领域:
化学
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材料科学
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能材料
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理论计算
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热分析
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）