JOURNAL OF RARE EARTHS
·
2023
·
IF 4.9
Danyu Zhang,Yuxuan Luo,Junyu Chen,Lianjie Li,Hai Guo
Dual-excitation and dual-emission Y 4 GeO 8 :Bi 3+ ,Sm 3+ phosphors were manufactured by traditional solid-phase sintering technique. The X-ray diffraction, morphology, photoluminescence, energy transfer process and temperature sensing properties of Y 4 GeO 8 :Bi 3+ ,Sm 3+ samples were comprehensively evaluated. The Y 4 GeO 8 :Bi 3+ ,Sm 3+ phosphors exhibit characteristic emissions of Bi 3+ ( 3 P 1 → 1 S 0 ) and Sm 3+ ( 4 G 5/2 → 6 H) under both 290 and 347 nm excitations. In fluorescence intensity ratio and Commission International de L'Eclairage coordinates modes, Y 4 GeO 8 :Bi 3+ ,Sm 3+ samples present excellent temperature measurement performance. The maximum relative sensitivity ( S r-max ) values of the former are 1.55 %/K (460 K, 290 nm excitation) and 0.82 %/K (506 K, 347 nm excitation). The S r-max ( x ) values of the latter are 0.21 %/K (437 K, 290 nm excitation) and 0.15 %/K (513 K, 347 nm excitation). These results illustrate that Y 4 GeO 8 :Bi 3+ ,Sm 3+ phosphors can be used as a candidate material for a dual-mode optical thermometer under dual-excitation.
研究领域:
材料科学
·
光学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Wenfang Liu,Haishuang Jia,Jie Zhang,Ling Shao,Jun Wang,Dawei Fang
In this work, a novel dual-excitation and dual-emission fluorescent probe , CDs–COO–Fluorescein (CDs–COO–F), for the specific detection of hydrazine (N 2 H 4 ) was designed and synthesized through esterification reaction of carbon dots (CDs) and fluorescein (F). The prepared CDs were characterized by TEM, XPS , XRD and FT-IR and the optical properties of CDs and CDs–COO–F were described by UV–vis and fluorescence spectra. On the other hand, the affecting factors including hydrazine (N 2 H 4 ) concentration and interfering substances on the hydrazine (N 2 H 4 ) detection were researched. The high sensitivity and high selectivity of CDs–COO–F in detection hydrazine (N 2 H 4 ) could be confirmed. Two good linear responses of fluorescence emission intensities of CDs–COO–F at 462 nm and 515 nm as functions of hydrazine (N 2 H 4 ) concentration were obtained, respectively. The limits of detection hydrazine (N 2 H 4 ) were 0.504 μM and 0.269 μM under 370 nm and 480 nm excitation wavelength lights, respectively. The detecting mechanism of hydrazine (N 2 H 4 ) by CDs–COO–F was deduced from the experimental results. Cell imaging and cytotoxicity experiments of CDs–COO–F were measured. The experiment results show that the fluorescent probe CDs–COO–F can be applied to detect hydrazine (N 2 H 4 ) in aqueous solution and living cells.
研究领域:
分析化学
·
生物医学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yan Zhao,Xinquan Yu,Xiao Liu,Dailiang Zhang,He Li,Hanlin Zhou,Weiheng Kong,Fengli Qu
Single-excitation ratio fluorescent probes have enabled the output signal with high signal-to-noise ratio, but are still plagued with technique challenges, including signal distortion and limited application scenario. Herein, a dual-excitation near-infrared (NIR) fluorescent probe P1 of coumarin derivatives is constructed, showing high signal output ability in the visible region and high tissue penetration depth ability in the NIR region. As NIR probe P1 selectively recognizes ClO–, the emission signal in the visible region (480 nm) of P1 is enhanced during the recognition process. Meanwhile, the NIR emission (830 nm) of the conjugated system is weakened, finally realizing that ClO– triggered the dual-excitation (720/400 nm) ratio fluorescence signal detection and monitoring. The signal of detection in vitro has high responsiveness. Meanwhile, in the process of NIR monitoring in vivo, positive contrast imaging of fluorescence is constructed, which can accurately monitor ClO– changes over time. The current dual-excitation fluorescence-based data calibration and/or comparison method improves the application of the traditional single-excitation ratio fluorescence strategy and provide innovative detection tools for accurate measurement of fluorescence detection, with detection/monitoring modes suitable for different physiological environments.
研究领域:
生物化学
·
医学影像
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Inorganics
·
2023
·Vol. 11
·期 6
·226
·
IF 2.9
Ya-Ting Gao,Shuai Chang,Bin-Bin Chen,Da-Wei Li
Constructing optical nanoprobes with superior performance is highly desirable for sensitive and accurate assays. Herein, we develop a facile room-temperature strategy for the fabrication of green emissive carbon nanoclusters (CNCs) with dual-exciting centers for the dual-channel sensing of hemin. The formation of the CNCs is attributed to the crosslinking polymerization of the precursors driven by the Schiff base reaction between ethylenediamine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. Most importantly, the proposed CNCs have a unique excitation-independent green emission (518 nm) with two excitation centers at 260 nm (channel 1) and 410 nm (channel 2). The dual-exciting central emission can serve as dual-channel fluorescence (FL) signals for highly sensitive and reliable detection of hemin based on the inner filter effect. Because of the great spectral overlap difference between the absorption spectrum of hemin and the excitation lights of the CNCs in the two channels, hemin has a different quenching effect on FL emission from different channels. The dual-channel signals of the CNCs can detect hemin in the range of 0.075–10 μM (channel 1) and 0.25–10 μM (channel 2), respectively. These findings not only offer new guidance for the facile synthesis of dual-exciting central CNCs but also establish a reliable sensing platform for the analysis of hemin in complex matrixes.
研究领域:
纳米材料科学
·
分析化学
·
生物医学工程
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DOI
高影响因子
Biomedical Optics Express
·
2016
·Vol. 7
·期 6
·2174-2185
·
IF 3.344
Xuanyuan Wen,Baoju Wang,Ruitao Wu,Nana Li,Sailing He,Qiuqiang Zhan
Simultaneous deep macroscopic imaging and microscopic imaging is in urgent demand, but is challenging to achieve experimentally due to the lack of proper fluorescent probes. Herein, we have designed and successfully synthesized simplex Er3+-doped upconversion nanoparticles (UCNPs) with double excitation bands for simultaneous deep macroscopic and microscopic imaging. The material structure and the excitation wavelength of Er3+-singly doped UCNPs were further optimized to enhance the upconversion emission efficiency. After optimization, we found that NaYF4:30%Er3+@NaYF4:2%Er3+ could simultaneously achieve efficient two-photon excitation (2PE) macroscopic tissue imaging and three-photon excitation (3PE) deep microscopic when excited by 808 nm continuous wave (CW) and 1480 nm CW lasers, respectively. In vitro cell imaging and in vivo imaging have also been implemented to demonstrate the feasibility and potential of the proposed simplex Er3+-doped UCNPs as bioprobe.
研究领域:
生物医学工程
·
材料科学
·
光学成像
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 42
·16478-16489
·
IF 3.069
Wenfang Liu,Bingqiang Wang,Haishuang Jia,Jun Wang,Youtao Song
Based on the esterification of 2-(2-hydroxyphenyl)benzothiazole (HBT) and a rare-earth metal ion complex EuIII-dtpa, a novel dual-excitation fluorescence probe, EuIII-dtpa-bis(HBT), was developed for the detection of hydrazine (N2H4). The structure of dtpa-bis(HBT) was characterized via FT-IR and NMR, and its optical properties were studied via UV-vis absorption and fluorescence spectroscopy. Influencing factors including solution acidity, interfering substances and N2H4 concentrations were considered for the detection of N2H4 using EuIII-dtpa-bis(HBT). EuIII-dtpa-bis(N2H4) and HBT emit significant fluorescence at 470 nm (λex = 270 nm) and 480 nm (λex = 325 nm), respectively. The detection limit of EuIII-dtpa-bis(HBT) with N2H4 was 0.283 μM (14 ppb) and 0.182 μM (9 ppb) upon excitation at 270 nm and 325 nm, which is close to and lower than the EPA standard (10 ppb), respectively. The mechanism for the detection of N2H4 by EuIII-dtpa-bis(HBT) was deduced from the experimental results and theoretical calculations. Also, cytotoxicity and cell imaging experiments of EuIII-dtpa-bis(HBT) were performed. The experimental results showed that the fluorescent probe EuIII-dtpa-bis(HBT) can be applied to detect N2H4 in aqueous solution and living cells.
研究领域:
分析化学
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生物医学工程
·
环境科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yongda Wang,Zheng Hu,Jia Li,Yunhan Zhao,Meng Kou,Feng Qin,Zhiguo Zhang
A dual-excitation laser modulation technique for the sensitive measurement of photosensitizers in tumor is proposed. The technique is based on the difference in optical absorption properties of photosensitizers and biological tissues at 405 nm and 450 nm. The 405 nm and 450 nm laser beams, with the same modulation frequency and 180° phase difference, were coupled into a single fiber simultaneously. A periodic signal with a frequency of 1 kHz was generated in alternating excitation, and was processed by phase sensitive detectors. The weak photosensitizer fluorescence masked by tissue autofluorescence can be effectively separated through the proposed method, so that the final signal intensity is only related to the concentration of photosensitizer. The method with a sensitivity of 0.22 mL/ng was determined by measuring the low concentration of protoporphyrin IX (PpIX) solution, and the detection limit of instrument reached 0.5 ng/mL. The puncturable optical probe with a diameter of 675 µm ensures that PpIX in can be detected with spatial resolution, which was performed in the measurement of PpIX in tumor bearing nude mice .
研究领域:
生物医学工程
·
肿瘤学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yijian Zhao,Qiuyue Wang,Yani Li,Meiyi Liu,Xiaochen Qin,Zhaoyue Shen,Jvzhe Li,Zhiyi Yao
The development of a sensory signal amplification approach is very crucial for rapid and precise detection of aflatoxin B1 (AFB1). However, such approaches remain scarce due to the weak interactions between AFB1 and the sensing probes. Herein, the first example of a dual-excitation fluorescent platform for antibody-free AFB1 detection is reported, which is assembled by an ordered π–π stack of cationic perylene derivative (PTHA) and tris(2,2′-bipyridine)ruthenium(II) [Ru(bpy3)2+]. Taking advantage of stepwise assembly and multiple binding sites of the nanoprobe, its ability for capturing AFB1 is significantly improved driven by multiple noncovalent interactions. Interestingly, dual-excitation fluorescent sensing mode with signal superposition and self-calibration is activated in the supramolecular coassembly process. Under excitation of 365 nm and 440 nm, the platform exhibits specific recognition toward AFB1 and the limit of detection is determined to be 0.12 ng mL–1. Notably, the dual-excitation platform demonstrates exceptional sensitivity enhancements of 106-fold, revealing that the self-calibrated reference improves the sensitivity and accuracy of analytical method significantly. The applications of our platform not only crack the problem of precise AFB1 detection via supramolecular coassembly strategy but also provide a universal sensitization strategy for ultrasensitive analysis in complex environments.
研究领域:
分析化学
·
食品安全
·
生物传感
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Li Chen,Niu Shengjie,Yu Siwen,Li Hang,Zhang Shengrui,Cao Xiaoyan,Li Lihua,Dai Huiping,Sun Jiangjiang,Li Hua,Wang Qin
The freshness of food is directly connected with safety, hygiene, and human health. Accurate quantification of biogenic amine level is crucial for assessing the food quality. Therefore, a dual-excitation ratiometric fluorescent probe MCCN based on hemicyanine dye for the examination of biogenic amines was constructed. After the addition of biogenic amines to the MCCN solution, an elimination reaction occurred after a nucleophilic addition reaction to form Schiff base MC-CA , resulting in colorimetric and ratiometric fluorescence responses. The probe MCCN solution was blue. After successively adding biogenic amines (cadaverine, n-butylamine and spermidine), the fluorescence emission intensity of probe MCCN at 709 nm reduced under excitation at 650 nm, meanwhile a new fluorescence emission peak emerged at 545 nm gradually increased under excitation at 450 nm, accompanying by a visible color variation from blue to yellow. The probe MCCN also displayed remarkable selectivity toward biogenic amines over other analytes. Based on the above excellent characteristics, the probe MCCN was successfully utilized to detect biogenic amines in the extract supernatant of shrimp and fish during the spoilage process. In addition, photographing the MCCN -loaded food extract supernatant enabled the correlation of color parameters with storage time, affording an accurate and simple method to reveal food spoilage process. The excellent sensing performance makes the MCCN probe a convenient and accurate screening platform for real-time assessing food freshness.
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DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 37
·4246-4255
·
IF 2.896
Xiaofeng Li,Yuejing Bao,Xiaorui Dong,Lihong Shi,Shaomin Shuang
Dual-excitation and dual-emission carbon dots (CDs) have been prepared by simple one-step hydrothermal treatment of p-phenylenediamine and 5-aminosalicylic acid. The as-prepared CDs emit bright green fluorescence under excitation at 320–400 nm and bright orange fluorescence under excitation at 490–560 nm. Interestingly, the CDs can be employed as a dual-excitation and dual-emission fluorescent probe for Fe3+ detection in aqueous solution and living cells. Furthermore, the obtained CDs can function as a promising dual-excitation and dual-emission temperature sensor. Additionally, the CDs can be utilized for lysosomal targeting.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
生物传感器
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Jie Zhang,Haishuang Jia,Wenfang Liu,Jun Wang,Dawei Fang
Cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H 2 S) are essential active substance in human body. The identification and detection of biothiols and H 2 S are of great significance for the early diagnosis of certain diseases. In this paper, a new dual excitation and dual emission fluorescent probe , carbon quantum dot-O-7-nitrobenzo-2-oxa-1,3-diazole (CQDs-O-NBD), for the detection of biothiols and H 2 S was synthesized. The carbon quantum dots (CQDs-OH) (λ ex/em maxima = 377 nm/467 nm) were synthesized by a microwave-assisted method using hydroquinone as the carbon source. Through the reaction of the phenolic hydroxyl group of the carbon quantum dots and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), the CQDs-O-NBD was synthesized. Due to the static quenching (SQ) and the photo-induced electron transfer (PET) from CQDs to NBD, the fluorescence of the CQDs-O-NBD is very weak. After adding biothiols and H 2 S, the ether bond of the CQDs-O-NBD was broken through nucleophilic substitution reaction , which led to the release of the CQDs-OH (channel 1 is turned on) and generation of a new compound NBD-thiols (channel 2). The CQDs-O-NBD as a probe shows the high selectivity and sensitivity for Cys/Hcy/GSH/NaHS (LOD of 0.24 μM/0.21 μM/0.19 μM/0.18 μM) over other aminos and ions by channel 1. The new formed compounds from NBD and Cys/Hcy have strong fluorescence under 470 nm wavelength light excitation (channel 2 is turned on), while the formed compounds from NBD and GSH/NaHS have no fluorescence (no channel 2), so the CQDs-O-NBD can discriminate Cys/Hcy (LOD: 0.07 μM/0.06 μM) from GSH/NaHS. In addition, the CQDs-O-NBD shows low toxicity and can be applied to the detection of Cys in fetal bovine serum and imaging in living cells.
研究领域:
分析化学
·
生物化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ouwen Xu,Shuyu Wan,Yuheng Zhang,Jiawei Li,Xiashi Zhu
A dual-"on-off-on" fluorescent probe based on nitrogen-doped carbon quantum dots (BG-NCDs) with the dual-excitation property was developed, which had a blue emission peak at 398 nm and a green emission peak at 518nm when excited at 298nm and 404nm, respectively. BG-NCDs were characterized by TEM, XPS, and FT-IR. The results showed that (1) the Fe (III) and ascorbic acid (AA) could “turn off/turn on” the blue fluorescence of BG-NCDs, the blue fluorescence of BG-NCDs was used as an “on-off-on” fluorescent probe to detecting the Fe (III) and AA in the linear ranges of 0.04–20.00 mg/L and 0.57–10.00 mg/L, the LODs were as low as 0.012 mg/L and 0.19 mg/L; (2) the presence of Cu (II) and glutathione (GSH) could “turn off/turn on” the green fluorescence of BG-NCDs, the “on-off-on” process of green fluorescence of BG-NCDs could be established as a fluorescence probe to detecting Cu (II) and GSH in the linear ranges of 1.08–16.00 mg/L and 2.46–32.00 mg/L, with LODs of 0.36 mg/L and 0.86 mg/L. The method was applied to determination analytes in real samples with satisfactory results. BG-NCDs displayed low cytotoxicity and was multicolored imaging DU145 cells successfully.
研究领域:
材料科学
·
分析化学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）