SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Lei Shang,Xiao Wang,Wei Zhang,Li-Ping Jia,Rong-Na Ma,Wen-Li Jia,Huai-Sheng Wang
Ratiometric electrochemiluminescence (ECL) assays have attracted widespread attentions in biosensing owing to their precise measurements by eliminating the environmental interferences. However, they mostly needed two eligible luminophors, increasing the complexity of the systems and limiting their practical applications. Herein, using luminol as single luminophor, a dual-potential ratiometric ECL strategy was proposed to detect carcinoembryonic antigen (CEA). The luminol exhibited cathodic and anodic emissions on graphene-ionic liquid-platinum (GR-IL-Pt) composites and Ti 3 C 2 MXenes-Au NPs hybrids, respectively. Then, a sandwich ECL sensor was fabricated using GR-IL-Pt composites as matrix to immobilize the primary antibodies of CEA and Ti 3 C 2 MXenes-Au NPs hybrids as platform to load the secondary antibodies. With the presence of CEA, the ratio of anodic ECL to cathodic ECL (ECL anodic /ECL cathodic ) increased obviously, realizing sensitive ratiometric detection of CEA. In addition, the ECL anodic /ECL cathodic was independent with the concentrations of H 2 O 2 , greatly improving the test reliability. The developed ECL sensor exhibited a sensitive detection toward CEA, performing a wide linearity in the range of 0.1 pg mL −1 - 10 ng mL −1 with a low detection limit of 34.58 fg mL -1 (S/N = 3). Furthermore, this strategy exhibited a good practicality to detect CEA in human serums, providing a promising strategy in ECL bioanalysis.
研究领域:
生物传感
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医学诊断
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产品关联
DOI
高影响因子
Analytical Methods
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2021
·Vol. 13
·期 7
·903-909
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IF 2.896
Yiming Liu,Yanan Sun,Minli Yang
A new kind of convenient, low-cost double-potential ratiometric ECL sensing platform for the quantification of Cu2+ was developed with carbon nitride nanosheets (g-C3N4 NSs) and graphene quantum dots (GQDs) as ECL luminophores. g-C3N4 NSs mixed with multi-walled carbon nanotubes (MWCNTs) was immobilized on a glass carbon electrode (GCE) and produced strong cathodic ECL at a potential of −1.2 V (vs. Ag/AgCl), while GQDs in the solution gave anodic ECL at +2.5 V. MWCNTs were used here to amplify the ECL signal of g-C3N4 NSs. The addition of Cu2+ causes the cathodic ECL signal from g-C3N4 to decline, while the anodic ECL signal from GQDs remains unchanged. With the anodic ECL signal as an internal reference, a double-potential ratiometric ECL sensing platform for Cu2+ was established. The ratio of the cathodic signal intensity to anodic signal intensity showed a linear response to the Cu2+ concentration over a range from 5.0 × 10−10 to 1.0 × 10−6 mol L−1 and the detection limit was 0.37 nmol L−1 (3σ/S). Such a construction strategy alleviates the interference from the environment and therefore improves the detection accuracy of Cu2+. Compared with other methods for Cu2+ detection, this method is simpler and more sensitive.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Hongmei Chen,Han Zhang,Ruo Yuan,Shihong Chen
Generally, electrochemiluminescence (ECL) ratiometric assays were based on the energy transfer (ET) between an emitter and a metal nanomaterial or between two different emitters. The choice of suitable energy donor–acceptor pair and the distance dependence of ET would greatly limit the practical application of ratiometric assays. This work explored a novel double-potential ECL ratiometry without the ET for organophosphorus pesticides (OPs) analysis, in which, reduced graphene oxide-CdTe quantum dots (RGO-CdTe QDs) and carboxyl-conjugated polymer dots (PFO dots) were chosen as cathodic and anodic ECL emitters, and the reactant (dissolved O2) and the product (H2O2) in enzymatic reactions served as their coreactants, respectively. With the occurrence of the enzymatic reactions induced by the acetylcholinesterase (AChE) and choline oxidase (ChOx), the cathodic ECL signal from RGO-CdTe QDs was at “signal off” state due to the consumption of dissolved O2. Meanwhile, the anodic ECL signal from PFO dots was at “signal on” state due to the in situ generation of H2O2. In the presence of OPs, the cathodic ECL signal would increase while the anodic ECL signal would decline correspondingly due to the inhibition of OPs on the activity of AChE. Using the reactant and the product in enzymatic reactions as the coreactants of two different ECL emitters, we conveniently achieved the opposite change trend in two ECL signals for the ratiometric detection of OPs, which exhibited a greatly improved accuracy, reliability and sensitivity, thus, showing a great attraction for developing ECL ratiometric systems for the bioanalysis.
研究领域:
分析化学
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环境科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chao Li,Juan Lu,Yanjia Guo,Yujia Song,Ruidan Li,Hanyue Jiang,Wenzhuo Wang,Pengfei Han,Guangping Ma,Jing Wang,Li Tian
In this paper, a novel dual-potential ratiometric electrochemiluminescence (RECL) sensor was constructed and applied to the ultrasensitive detection of kanamycin (KAN). The RECL sensor was constructed using porous graphite phase carbon nitride (PCN) and luminol, which serve as the luminescent reagents at the cathode and anode, respectively. PCN not only functioned as the luminescent reagent for the cathode but also served as a carrier for the Pt Au NPs. Simultaneously, Ti 3 CN acted as a carrier for the anode luminescent reagent, luminol, thereby enhancing the ECL signals at anode. Under optimal conditions, the ratio of the anode to cathode ECL signals (I luminol /I PCN ) showing excellent linear relationship with the logarithm of KAN concentration (lgC KAN ) within the range of 5.0 × 10 −14 to 5.0 × 10 −8 mol/L, with a detection limit as low as 1.67 × 10 −14 mol/L, making it suitable for detecting KAN in milk samples.
研究领域:
分析化学
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食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Hongguo Zhai,Yue Wang,Lingjun Geng,Qi Guo,Yuhao Zhang,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
In this paper, an electrochemiluminescence (ECL) biosensor was constructed using Bi 2 S 3 @Au nanoflowers as the based nanomaterial and [email protected] and CdS QDs as independent ECL emission signal respectively. As the substrate of the working electrode, Bi 2 S 3 @Au nanoflowers improved the effective area of electrode and accelerated electron transfer rate between gold nanoparticles and aptamer, provided a good interface environment for the loading of luminescent materials. Then, the [email protected] functionalized DNA2 probe was used as an independent ECL signal source under positive potential and recognized Cd(II), while the CdS QDs functionalized DNA3 probe was used as an independent ECL signal source under negative potential and recognized ampicillin. The simultaneous detection of Cd(II) and ampicillin in different concentrations are realized. This sensor not only has good selectivity and high sensitivity in real sample detection, but also open up a novel way to construct multi-target ECL biosensor for simultaneous detection.
研究领域:
分析化学
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环境科学
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食品安全
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纳米材料
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Jie Huang,Zhirong Liu,Dejuan Huang,Tianxiang Jin,Yong Qian
In order to efficiently extract uranium from uranium-containing wastewater, a novel acid doped polypyrrole/carbon felt (PA-PPy/CF) electrode was prepared via a facile electrodeposition method. For this material, PA and PPy combined to form a stable chemical structure by a charge compensation mechanism. The electrochemical characterization results showed that PA-PPy can significantly accelerate the electrochemical reduction rate of uranium ions. Moreover, a double potential step technique (DPST) was applied to prevent water splitting and maintained the electrocatalytic reduction activity of the surface groups during the electrochemical adsorption process . The removal efficiency obtained by the DPST method was six times higher than that obtained by the conventional chemical adsorption . When the concentrations of uranyl nitrate were 10, 20, 50, and 100 mg/L, the removal efficiencies of uranium were 98.8%, 98.1%, 94.6%, and 93.7%, and the adsorption capacities of uranium were 164.7, 326.9, 788.5, and 1562.0 mg/g, respectively. This material also showed an excellent recycling performance and remarkable selectivity for uranium ions. This work may shed light on the development of removal system for uranium (VI).
研究领域:
环境科学
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材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）