FOOD CONTROL
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2025
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IF 6.3
Yanping He,Peixuan Li,Xin Zhao,Dongmei Wang,Fuqiang Zhang
The abuse or misuse of fleroxacin (FLE) in aquaculture and livestock may elevate FLE residues in foods, posing a serious threat to human health. Consequently, FLE detection has garnered extensive attention in food science. This study developed a magnetic separation-assisted colorimetric aptasensor for ultrasensitive FLE detection, leveraging the enhanced nanozyme activity of CoOOH nanoflakes with hairpins and dual cascade amplification (CHA-HCR). Under optimal experimental conditions, the proposed aptasensor exhibited high specificity for FLE with a wide linear range (1–100 nM) and a detection limit as low as 758 pM. Furthermore, the colorimetric aptasensor demonstrated robust performance in FLE-spiked honey analysis, achieving 96.70–101.06 % recoveries with 2.42–5.45 % RSDs, which showed strong agreement with commercial ELISA results. This study establishes a simple and effective platform for sensitive FLE detection in honey samples, demonstrating significant potential for practical food safety monitoring.
研究领域:
食品科学
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分析化学
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纳米技术
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环境卫生
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生物传感器
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产品关联
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 6
·1585-1595
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IF 3.641
Chen Ben-Qi,Yang Meng,Liao Wen-Chun,Li Zi-Ying,Chen Zhi-Xiong,Yang Yun-Hui,Yang Tong,Hu Rong
Developing a strategy for the determination of trace ochratoxin A (OTA) in food is very important to ensure food safety. Here, based on a tripod probe, an electrochemical aptasensing platform for detected OTA sensitively was designed, which used the 3D DNA walker by endonuclease Nb.BbvCI
powered and the nanozyme of porous Co3O4 nanodisks loaded Pt nanoparticles (Pt NPs@Co3O4) as a cascaded signal amplifying device. With OTA as a model target, the swing-arm probe of DNA walker was triggered. Next, probe on the magnetic beads was cleaved
and released by Nb.BbvCI to realize the first signal amplification. After that, the partial sequence of the released probe is well assembled with triple-strand DNA by strand reaction and the other sequence exposed as a capture probe was combined with the Pt NPs@Co3O4.
It can efficiently make the decomposition of H2O2, bring about significant current, and the signal was amplified again. The designed electrochemical aptasensor demonstrated outstanding detection linearity in the OTA concentration range of 1 pg/mL to 100 ng/mL, with a
detection limit of 0.194 pg/mL (S/N = 3), with excellent reproducibility and high specificity. It can be used to find OTA in food. This research supplies a potential split-new avenue for the sensitive detection of electrochemical aptasensor.
研究领域:
分析化学
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纳米技术
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食品科学
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生物化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Yuhao Weng,Xiaoqian Chen,Huihong Chen,Huilin Yang,Zhigang Gong,Hongliang Tan
Catalytic bacterial inactivation mediated by peroxidase nanomimics, which converts hydrogen peroxide (H 2 O 2 ) to hydroxyl radical (•OH) with high toxicity, has been emerged as a promising solution to combat bacterial infections and antibiotic resistance. However, the bacterial inactivation efficacy was often restricted by the low catalytic activity of peroxidase nanomimics and the short half-life and limited diffusion distance of •OH. Here, we developed a hydrogel microreactor (GHAP) integrated double cascade reactions to sequentially generate •OH and nitric oxide (NO) for synergistic bacterial inactivation, where NO offers a complementary effect to compensate •OH for incomplete bacterial inactivation. The GHAP was fabricated by the coencapsulation of glucose oxidase (GOx), hemoglobin (Hb) and zeolitic imidazolate framework-8 loaded with arginine (Arg) in polyacrylamide (pAAm) hydrogel microsphere. The presence of glucose can trigger GHAP to sequentially generate •OH and NO though the successive coupling of GOx with Hb and Arg. The pAAm microsphere was demonstrated to enhance cascade catalytic efficiencies and shield GHAP against harsh conditions due to its confinement effect. Importantly, compared with single •OH/NO-generated cascade systems, the superiority of GHAP was highlighted by the synergistic antibacterial effects of •OH and NO, which thereby endows GHAP with a greatly enhanced antibacterial performance and an exceptional broad-spectrum antibacterial ability. In vivo experiments in a mouse model demonstrated that GHAP could be used for wound disinfection with minimal side effects. This work opens a new avenue for the rational design of biomimetic reactors for biomedical applications.
研究领域:
生物医学工程
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材料科学
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微生物学
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药剂学
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DOI
高影响因子
Small
·
2022
·Vol. 18
·期 12
·2106083
·
IF 15.153
Yu Gao,Wenzhan Xu,Si-Wei Zhang,Tianjie Fan,Meng Zhang,Aihua Ran,Xuan Zhang,Feiyu Kang,Guodan Wei
Nowadays, nearly 48.7% near-infrared (NIR) irradiation (>800 nm) of the full solar spectrum has actually not been fully utilized since the state-of-the-art perovskite film usually can only absorb the most UV–vis sunlight radiation. Herein, high efficiency integrated Cs 0.15 FA 0.85 PbI 3 perovskite/organic bulk (PC 61 BM:D18:Y6) heterojunction solar cells with enhanced low energy photon harvest until 931 nm and a high maintained open circuit voltage of 1.04 V is successfully obtained. In particular, the favorable double cascading charge transfer paths pave an interesting possibility to spatially separate electrons upon visible light excitation and holes upon NIR photon absorption simultaneously at interfaces, significantly suppressing non-radiative bimolecular recombination and reaching the photocurrent density as high as 27.48 mA cm −2 and power conversion efficiency of 20.31%. Besides, the strong hydrophobicity of the ternary organic film has effectively prevented ambient humidity penetration and improves the stability of the perovskite in the continuous aging test (humidity > 60%) compared with the control device. This work has opened a significantly new window to improve the NIR light harvest for next generation highly efficient solar cells with full spectrum response.
研究领域:
材料科学
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太阳能电池
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光伏技术
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有机电子学
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钙钛矿太阳能电池
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近红外光吸收
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DOI
高影响因子
危险品化学品经营许可证（带存储）