Analytical Methods
·
2024
·
IF 2.7
Chu Cheng,Qingzhou Cheng,Wei Zhou,Yulong Chen,Wenbin Liu,Zhiling Zhang,Jingsi Ye,Pengfeng Xiao
Eliminating errors in next-generation sequencing has proven to be challenging. Here we present a novel strategy for DNA sequencing, called correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry. This sequencing method involves introducing a mixture of natural nucleotide X, labeled unblocked nucleotide X′, 3′ blocked nucleotide Y*, and labeled 3′ blocked nucleotide Y* into each reaction cycle. By cyclically interrogating a template twice with different nucleotide combinations, two sets of base-encoding are sequentially obtained, enabling accurate deduction of base sequence. We demonstrate the remarkable efficacy of this approach in detecting and correcting sequencing errors, achieving a theoretical error rate of 0.0005%, which is twice as accurate as Sanger sequencing. Furthermore, we show the capability to detect known mutation sites using information from only a single sequencing run. The correctable two-color fluorogenic DNA decoding sequencing approach should enable accurate identification of extremely rare genomic variations in diverse applications in biology and medicine.
研究领域:
分子生物学
·
基因组学
·
医学遗传学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 3
·1258-1264
·
IF 4.036
Zengchen Liu,Like Wang,Baodui Wang,Yahong Chen,Fengshou Tian,Yingying Xue,Yanxia Li,Wenping Zhu,Weijie Yang
As a class of important carbon nanomaterial, carbonized polymer dots (CPDs), also called carbon dots (CDs), have aroused wide interest owing to their unique water solubility, fluorescence properties, and rich surface functional groups. However, the directional tuning of the fluorescence properties of CPDs remains incomplete because of the influence of many factors like diameter, solvent and surface groups. Particularly, most carbonized polymer dots are synthesized in a neutral pH environment. Herein, by modulating the pH (strongly acidic or alkaline) of dextrin water solution, bicolor fluorescence emission (blue and yellow) CPDs were prepared by a hydrothermal reaction. Through systematic characterization, it was found that the different fluorescence properties are regulated by the diameters and surface groups of the carbon cores. Simultaneously, the pH value affected the nucleation process. Based on the excellent fluorescence properties, cell fluorescence imaging and cytotoxicity were tested. The bicolor fluorescence CPDs obtained by tuning the pH provide an important theoretical basis for the design of broadband CPDs.
研究领域:
材料科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Xin Zhang,Beibei Wang,Zhiyu Zheng,Guiqiang Yang,Chao Zhang,Liqiong Liao
Stimuli responsive fluorescence characteristics are significant in some fields, such as sensors, information encryption , and bionic camouflage. In this work, a robust poly(vinyl alcohol) (PVA) hydrogel with photoswitchable dynamic fluorescence behaviors was constructed by incorporating dual-color fluorescent nanoparticles (DCFNs). The DCFNs were composed of the spiropyran-modified β-cyclodextrin (β-CD-SP) and nitrobenzoxadiazolyl (NBD) derivatives, which could exist in the PVA network through non-covalent interaction. The SP moiety functioned as an acceptor and realized the photoisomerization to further quench or recover the fluorescence of the NBD moiety based on the fluorescence resonance energy transfer (FRET) effect. Therefore, under the irradiation of ultraviolet light (UV) or visible light (Vis), the hydrogel showed a fluorescence emission peak of merocyanine (MC) at 660 nm (red) or NBD at 550 nm (green), respectively. Additionally, the pH responsive characteristic of the MC chromophore also endowed the hydrogel with pH responsive fluorescence behaviors. Utilizing these advantages, specific patterns can be displayed on the hydrogel in dual mode through a hollowed-out photomask. The hydrogel could also be utilized as dual-color “building blocks” to construct various 2D or 3D structures. This composite hydrogel has potential application in various fields, such as sensors, optical switch , information storage, and biomimetic components.
研究领域:
材料科学
·
生物传感
·
仿生学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 44
·15697-15704
·
IF 7.059
Mengyu Deng,Yangdaiyi Zhang,Yufei Wang,Shimei Jiang
A new kind of photoswitchable polymer nanoparticle is prepared using polymerizable alkenyl carbon dots (PCDs) and a spiropyran derivative, together with methyl methacrylate (MMA) as the monomer, and mediated by one-pot miniemulsion polymerization. Switching between two different wavelengths of emitted light can be realized by intraparticle fluorescence resonance energy transfer (FRET). The FRET efficiency could be easily controlled by the feed ratios of the two chromophores and the excitation wavelengths. UV and visible light can be applied to modulate the fluorescence emission of the prepared nanoparticles. Upon visible light irradiation, the nanoparticles emit blue-green fluorescence of the CDs while spiropyran is in its closed form (SP). UV irradiation of SP gives rise to an open-ring isomer (MC). An SP-to-MC photoisomerization triggers a CD-to-MC FRET, and the resulting nanoparticles emit red light. Their fluorescence could be reversibly switched between blue-green and red by using UV/vis light irradiation with excellent photoreversibility and high stability. In addition, the novel nanoparticles with reversibly photoswitchable fluorescence could be directly applied in rewritable fluorescence patterning and dual-color fluorescence imaging of living cells.
研究领域:
材料科学
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生物医学工程
·
光学
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产品关联
DOI
高影响因子
Oncotarget
·
2017
·
IF 5.168
Guang Yang,Yong Cao,Bin Yan,Qiang Lv,Jianbo Yu,Fusheng Zhao,Zhihong Chen,Heran Yang,Mengxi Chen,Zaishun Jin
Upconversion nanoparticles are a new type of fluorescent marker in biomedical imaging that can convert a longer wavelength (such as near-infrared fluorescence) into a shorter wavelength (such as visible light). Mantle cell lymphoma, which is derived from B-cell lymphoma, is a subtype of non-Hodgkin's lymphoma, and the immune phenotype is a mature B-cell phenotype (CD20+, CD5+). To develop the use of nanomaterials as specific markers for the medical imaging of mantle cell lymphoma, we modified the surface of UCNPs by oxidation so that the CD20 or CD5 antibody could covalently attach to the upconversion nanoparticles to form antibody-UCNP conjugates. These antibody-UCNP conjugates were used as fluorescent probes to detect the CD20 or CD5 antigen. Due to the excessive expression of these antigens on the surface of MCL cells and successful strong connection between the antibody and UCNPs, the latter could specifically combine with mantle cell lymphoma cells. Upon near-infrared excitation at 980 nm, cells labelled with UCNPs emitted bright upconversion fluorescence.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yingao Yang,Ruipeng Chen,Yifen Guo,Jiaxin Zhang,Shuyue Ren,Huanying Zhou,Zhixian Gao
A two-color fluorescent sensing strategy based on a functionalized tetrahedral DNAzyme nanotweezer (FTDN) was developed to detect ochratoxin A (OTA) utilizing the multifunctional properties of DNA nanotechnology. The FTDN enables rapid OTA detection directly through a Cy5 fluorescent group, modified to respond to the target signal. Additionally, FTDN exhibits DNAzyme cutting activity in the presence of Mg 2 ⁺ ions, enabling it to traverse DNA nanoflower-functionalized magnetic beads. This process results in the continuous cleavage of DNA nanoflowers labeled with numerous FAM fluorescent groups, thereby amplifying the detection signal and enhancing OTA sensitivity. The linear ranges for the Cy5 and FAM signals in response to OTA were 5–1000 ng/mL and 0.05–100 ng/mL, respectively, with corresponding limits of detection (LOD) of 1.59 ng/mL and 0.03 ng/mL. This study demonstrates that dual-color fluorescence via Cy5 and FAM can effectively verify OTA detection in food, significantly reducing false-positive and false-negative rates. The proposed platform offers sensitive and accurate detection of mycotoxins in food and can be adapted for monitoring other trace contaminants by simply altering the aptamer.
研究领域:
分析化学
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纳米技术
·
食品科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 8
·期 1
·240-246
·
IF 6.641
Xiaobin Wang,Zhuoyi Li,Wen Ying,Danke Chen,Peipei Li,Zheng Deng,Xinsheng Peng
Fluorescent proteins (FPs) with good photostability and outstanding photoluminescence features are very promising luminous materials for the fabrication of white-light-emitting diodes (WLEDs). However, the requirement of an aqueous environment and poor thermal stability have strongly restricted their wide applications in lighting devices. In this paper, we present a facile strategy to encapsulate R-phycoerythrin (R-PE) fluorescent proteins into a blue metal organic framework (MOF) HSB-W1 (HSB = hydrogenated Schiff base) thin film through a facile solid-confinement conversion process. As a result, R-PE proteins embedded into HSB-W1 crystals are denatured, but exhibit dual color fluorescence emissions including green (518 nm) and red (600, 647 nm) light, while the original single orange light (578 nm) is significantly suppressed. After careful adjustment of R-PE content, the resulting R-PE@HSB-W1 thin film emits high-quality white light with nearly ideal Commission Internationale de I’Eclairage (CIE) coordinates of (0.33, 0.34), a high color rendering index (CRI) value of approximately 85 and a moderately correlated color temperature (CCT) value of 5740 K. Such a strategy can be widely utilized for other fluorescent molecules and luminescent MOFs to design white-light-emitting materials.
研究领域:
材料科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 11
·14932-14943
·
IF 2.478
Deng Huawei,Liu Jun,Zhang Hang,Li Chaorui,Liu Zhen,Chen Dihu
Multimodal imaging has attracted tremendous attention in biotechnology and nanomedicine applications. Monoclinic Gd 2 O 3 :Dy 3+ nanoparticles (NPs) with dual-color fluorescence imaging (FI) and magnetic resonance imaging (MRI) have been successfully synthesized by employing laser ablation in liquid (LAL) technique. The synthesized nanoparticles can be used as yellow fluorescence nanoprobe as well as blue ones due to their sharp and strong yellow and blue emissions. Magnetic resonance imaging (MRI) measurement shows that the longitudinal relaxivity (r 1 ) of the Gd 2 O 3 :1%Dy 3+ NPs is 4.5 times higher than that of the commercial Gd-DTPA. The experiment of in vitro cell fluorescence imaging and magnetic resonance imaging of female Balb/c mouse indicates that the Gd 2 O 3 :Dy 3+ NPs synthesized by LAL technique are promising candidates of dual-color FI nanoprobe and MRI contrast agent in further clinical applications.
研究领域:
生物技术
·
纳米医学
·
材料科学
·
医学成像
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 3
·675-682
·
IF 4.2
Chenhui Hao,Jinxiu Wei,Shenfei Zong,Zhuyuan Wang,Hong Wang,Yiping Cui
Ag+ ions are widely used in various fields of human life due to their unique properties and they threaten the environment and human health. The traditional methods for Ag+ detection commonly suffer from disadvantages including limited sensitivity, expensive equipment and complicated operating steps. Herein, we developed a highly specific dual-color fluorescence co-localization (DFC) strategy based on the C–Ag+–C structure for Ag+ detection. In this strategy, Ag+ ions can be captured to form C–Ag+–C base pairs, and these ions enable single-stranded DNAs to form double strands. The DFC strategy can exclude nonspecific interaction sites and greatly improve the sensitivity and specificity. By DFC of the QDs and Cy5 linked to the DNA strands, highly sensitive Ag+ detection was achieved in the concentration range from 0.14 pM to 200 nM, with a limit of detection (LOD) of 0.14 pM. Moreover, this method has been applied for the detection of Ag+ ions in real environmental samples with satisfactory recoveries. We believe that the DFC strategy is promising for Ag+ detection.
研究领域:
分析化学
·
环境科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Liuyue Xiao,Tianhao Li,Hui Ling,Yanjing Yang,Shian Zhong
The hybrid ratiometric fluorescence sensors (GKHMIPs & RKHMIPs) encompass luminescent substances incorporated within a core-shell architecture. These sensors manifest a diverse array of hues in response to variations in the concentration of alpha-fetoprotein, accomplished through disparities in the quantum dots (QDs)-protein response. This study is devoted to comprehending the principles governing the alteration of fluorescence during the recognition process of the ratiometric fluorescence sensor. A compensation mechanism, rooted in the correlation between fluorescence and protein response, is postulated through a fluorescence interaction model (multi-FRET model). Multi-FRET model can be divided into FRET process and Fluorescence compensate process (FCP), FCP occurs when electron orbits are filled, hindering the fluorescence resonance energy transfer process and leading to the return of excited state electrons to the ground state by alternative pathways. Based on the multi-FRET model we demonstrate the Sherpa formular to calculating the fluorescence-target relation. To assess the visual impact, the CIE-Lab model is introduced to validate the rationale behind the multi-color colorimetric model. The influence of different mixing ratios on the visualization effect is elucidated using the concentration fluorescence response model, and the optimal relationship between wavelength combinations in the tricolor mixing model is proposed.
研究领域:
生物传感器
·
分析化学
·
分子生物学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）