RSC Advances
·
2014
·Vol. 4
·期 16
·8191-8199
·
IF 3.708
Haiyan Chen,Min Zhang,Haibo Yang,Weixia Xu,Yuxiang Ma,Yueqing Gu
The unique properties of gold nanoclusters (Au NCs) enable them to be functionalized as fluorescent probes or pro-drugs for biomedical application. A facile approach was introduced to synthesize nanoconjugates (Au–cRGD–MPA), which was composed of Au NCs, cyclic (Arg–Gly–Asp–DTyr–Lys) (cRGD) peptide and a near infrared (NIR) fluorescence dye (MPA). The nanoconjugates exhibited dual fluorescence in the visible range and NIR range simultaneously. The low cytotoxicity of Au–cRGD–MPA was demonstrated in MCF-7 cell line and U87 cell line. The cell affinity investigations verified the outstanding tumor-targeting capability of Au–cRGD–MPA for tumor cells. The biodistribution characteristics and tumor targeting ability of the probes were elucidated in normal nude mice and tumor-bearing mice, respectively. Attributing to the small size (less than 5 nm), the majority of the nanoconjugates metabolized from the living subjects via the kidney-bladder pathway. Additionally, Au–cRGD–MPA displayed favorable tumor-specificity in Integrin αvβ3 over-expressed tumor-bearing (MDA-MB-231) mice. Consequently, the results demonstrated that the dual fluorescence probe is a promising candidate for tumor imaging and disease treatment monitoring.
研究领域:
生物医学工程
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分子影像学
·
纳米医学
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产品关联
DOI
高影响因子
MOLECULAR PHYSICS
·
2024
·
IF 1.6
Gang Wang,Lina Ding,Yang Liu,Yonggang Yang,Yufang Liu
2-(2'-Hydroxyphenyl) benzimidazole (HBI) and its derivatives are widely used in fluorescent probes, luminescent materials, and other sensing materials. In this study, the time-resolved fluorescence behaviour, and excited-state dynamics of HBI and 2-phenyl-1H-benzo[d]imidazole (BI) were studied using femtosecond transient absorption (fs-TA) spectroscopy. The stimulated emission (SE) and excited-state absorption signals of the BI molecule were located at 355 and 437 nm, respectively. For HBI, these signals were located at 475 and 384 nm, respectively. The SE signals of the BI and HBI molecules in their fs-TA spectra were consistent with the experimental short-wave fluorescence at 340 and 460 nm, respectively, in the steady-state spectra. The long-wave signal of HBI at 475 nm indicates its dual fluorescence, which may be caused by proton transfer or conformational torsion after photoexcitation. The large theoretical energy barrier of 40.97 kcal/mol in first excited (S1) state of HBI excludes the latter hypothesis. Additionally, the small energy barrier (1.88 kcal/mol) in the S1 state confirmed the excited-state intramolecular proton transfer (ESIPT). This indicates that ESIPT induces dual fluorescence behaviour of the HBI molecule, which was also demonstrated by the infrared spectra, theoretical fluorescence peaks, and molecular orbitals.
研究领域:
光化学
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物理化学
·
理论化学
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DOI
高影响因子
Soft Matter
·
2018
·Vol. 14
·期 22
·4495-4504
·
IF 3.709
Afeng Yang,Xia Dong,Jie Liang,Yan Zhang,Wenzhi Yang,Tianjun Liu,Jun Yang,Deling Kong,Feng Lv
The real-time tracking and adjustment of the disassembly and status of hydrogels in vivo are important challenges to accurate and precise assessment. In this article, a photothermally controllable, visible, dual fluorescent thermosensitive hydrogel was designed and developed based on a porphyrin–poly(ethylene glycol)/IR-820–α-cyclodextrin hydrogel. Due to the photothermal effect and fluorescence emission of IR-820, it can exert the dual functions of photothermal control and fluorescence imaging tracking. The IR-820 conjugated hydrogel can regulate the hydrogel disassembly by the photothermal effect of IR-820. Furthermore, each component of the hydrogel can be tracked by the fluorescence of IR-820 and porphyrin. Fluorescence imaging tracking and remote photothermal control were merged into the visible and controlled hydrogel disassembly after subcutaneous injection using mice as models. The dual fluorescence imaging visualization of cyclodextrin/poly(ethylene glycol) hydrogels revealed the disassembly process by tracking each component, and the hydrogel disassembly can be efficiently accelerated under laser irradiation with the photothermal effect of IR-820. This affords an important basis for understanding the disassembly process of the poly(ethylene glycol)/α-cyclodextrin hydrogel.
研究领域:
生物材料学
·
医学工程学
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DOI
PubMed
高影响因子
Small
·
2017
·Vol. 13
·期 26
·1700075
·
IF 8.643
Jiangling He,Youling He,Yonghao Chen,Bingfu Lei,Jianle Zhuang,Yong Xiao,Yeru Liang,Mingtao Zheng,Haoran Zhang,Yingliang Liu
Stable solid-state red fluorescence from organosilane-functionalized carbon dots (CDs) with sizes around 3 nm is reported for the first time. Meanwhile, a novel method is also first reported for the efficient construction of dual-fluorescence morphologies. The quantum yield of these solid-state CDs and their aqueous solution is 9.60 and 50.7%, respectively. The fluorescence lifetime is 4.82 ns for solid-state CDs, and 15.57 ns for their aqueous solution. These CDs are detailedly studied how they can exhibit obvious photoluminescence overcoming the self-quenching in solid state. Luminescent materials are constructed with dual fluorescence based on as-prepared single emissive CDs (red emission) and nonfluorescence media (starch, Al 2 O 3 , and RnOCH 3 COONa), with the characteristic peaks located at nearly 440 and 600 nm. Tunable photoluminescence can be successfully achieved by tuning the mass ratio of CDs to solid matrix (such as starch). These constructed dual-fluorescence CDs/starch composites can also be applied in white light-emitting diodes with UV chips (395 nm), and oxygen sensing.
研究领域:
材料科学
·
光学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Huayin Zhang,Lanjing Wang,Qixun Nian,Shiwen Liu,Jingjing Li,Qian Xu
Spiropyran-functionalized graphitic carbon nitride (MCH@g-C3N4) in acidic conditions was prepared for the first time. MCH@g-C3N4 exhibited dual-wavelength fluorescence emission with two distant bands and distinct solvatochromic behavior owing to the different molecular structures of spiropyran. A three-dimensional organic solvent decoder was fabricated for solvent identification based on the emission wavelength and fluorescence quantum yield of MCH@g-C3N4 in different polar solvents. The ratiometric fluorescence sensing of the water content in organic solvent was realized using water to induce the structural change of spiropyran. A solid-phase MCH@g-C3N4/polyvinylpyrrolidone (PVP) nanofiber mat-based sensor was prepared via cospinning MCH@g-C3N4 with hydrophilic PVP. A fluorescent humidity sensor was fabricated using a commercial syringe equipped with a stainless-steel needle (catcher) to obtain the air inside the grain heap, which enabled the accurate location of the sampling spot. The space between the syringe and piston was used as an enclosed sensing space. Thus, a simple, accurate, and intuitive visual colorimetric method for the internal humidity of a grain heap was realized by analyzing the recorded images.
研究领域:
材料科学
·
传感技术
·
农业科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2018
·
IF 1.703
Yu Wu,Yun Huang,Yulan Wang,Xiang Zou,Jun Wang,Weiping Wu
Developing a fast and sensitive method for the detection of 2,4,6-trinitrophenol (TNP) or Cr2O72– in aqueous solution has received great attention. In this work, a regenerable fluorescent coordination polymer [Zn(L)(H2L)]n (1) has been successfully constructed using a novel Schiff base ligand N'-[(E)-(3,5-dibromo-2-hydroxyphenyl)methylene]isonicotinohydrazide(H2L) and exploited as a dual detection probe for the selective sensing of Cr2O72– and TNP in water media. Competitive fluorometric experiments involving mixtures of anions or nitro compounds demonstrated 1 as an efficient and selective sensor for both Cr2O72– and TNP. The limits of detection for Cr2O72– and 2,4,6-trinitrophenol in aqueous solutions were found to be 11.4 and 1.19 μM, respectively. Moreover, 1 can detect TNP and Cr2O72– in real water samples. All these features clearly demonstrate its great potential of practical TNP and Cr2O72– detection in water media for public safety and security.
研究领域:
材料科学
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yanan Li,Jian Cao,Chuanxiao Wang,Yiyi Qiao,Jiayu Gao,Linlin Wang,Xiao Zhang,Le Wang,Yuanhao Li,Zhicheng Zuo
Serum albumin is the most abundant protein in mammalian plasma. It occupies an extremely important position in organisms as an early indicator of the development of many diseases. The development of fluorescence sensing technology has revolutionized the drawbacks of traditional methods. In this strategy, we designed and synthesized three dual fluorophore organic sensors TPA-IPA-T1, TPA-QL-T2, and TPA-Py-T3 for the detection of serum albumin. The rotation of the C–C bond of sensors is restricted and the molecular structure forms a rigid plane that contributes to the fluorescent emission intensity of the sensor. The fluorescent skeletons were successfully constructed by triphenylamine composed the electron-withdrawing nitrogen-containing compounds benzopyrrole (indole), benzopyridine (quinoline), and pyridine, respectively, with piperazine as a rigid connecting bridge. The addition of human serum albumin (HSA) turned on the fluorescent emission of the sensor near 580–600 nm. The color of the sensor solution changed from colorless to pink and bright orange, respectively. The fluorescent intensity increased by 24, 67, and 31 fold, respectively. The sensors have the advantages of sensitivity and fast response, strong anti-interference ability, low detection limit and good water solubility. Drug experiments in artificial urine demonstrate the characteristics of sensors, which has a very high potential for biological feasibility and medical applications. The sensors facilitaty the binding of the HSA through electrostatic interactions. The sensors were explored to be able to efficiently bind the IB structural domain of HSA, which had a good meaningful of avoiding the interference of commonly drugs in clinical medicine and in artificial urine on the detection of HSA.
研究领域:
生物化学
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医学诊断
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分析化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 1
·297-306
·
IF 3.719
Liang Wanjun,Liu Zhengqing,Li Dan,Wu Xiaoping,Liu Shaopu,He Youqiu
We describe a novel fluorescent bioprobe for the sensitive and selective detection of double-stranded DNA (dsDNA). It consists of quantum dots (Q-dots) whose fluorescence is quenched (through photoinduced electron transfer) on covering them with the Al(III)-pefloxacin complex. It is found, however, that dsDNA has an affinity for this complex that is so strong that it can break up the binding to the quantum dots, this leading to a complete recovery of the fluorescence of both the Q-dots and the Al(III)-pefloxacin complex. Dual fluorescence (peaking at 420 and 547 nm) is observed under a single excitation wavelength of 350 nm. Neither ribonucleic acid, bovine serum albumin, nor biologically relevant metal ions are capable of giving this effect. The detection limit (3σ/k) for herring sperm DNA is 15.3 μg L −1 if determined via the fluorescence of the Q-dots, and 48 μg L −1 via the Al(III)-pefloxacin complex. The method was applied to the determination of hsDNA in synthetic samples and gave excellent results.
Al 3+ -pefloxacin mesylate (QDs-Al 3+ -PFLX) complex acts as an excellent quencher of the fluorescence of QDs, which was attributed to the photoinduced electron transfer from QDs to Al 3+ -PFLX complex. Due to the strong and specific binding of double-stranded DNA with Al 3+ -PFLX complex, the fluorescence intensity of QDs-Al 3+ -PFLX dyads system is restored
研究领域:
生物化学
·
分子生物学
·
分析化学
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产品关联
DOI
高影响因子
Nanomaterials
·
2021
·Vol. 11
·期 12
·3283
·
IF 5.076
Wenbin Yang,Siqi Niu,Yao Wang,Linjun Huang,Shichao Wang,Ketul C. Popat,Matt J. Kipper,Laurence A. Belfiore,Jianguo Tang
In this work, titania (TiO2) nanoparticles modified by Eu(TTA)3Phen complexes (ETP) were prepared by a simple solvothermal method developing a fluorescence Mn7+pollutant sensing system. The characterization results indicate that the ETP cause structural deformation and redshifts of the UV-visible light absorptions of host TiO2nanoparticles. The ETP also reduce the crystallinity and crystallite size of TiO2nanoparticles. Compared with TiO2nanoparticles modified with Eu3+(TiO2-Eu3+), TiO2nanoparticles modified with ETP (TiO2-ETP) exhibit significantly stronger photoluminescence under the excitation of 394 nm. Under UV excitation, TiO2-ETP nanoparticles showed blue and red emission corresponding to TiO2and Eu3+. In addition, as the concentration of ETP in TiO2nanoparticles increases, the PL intensity at 612 nm also increases. When ETP-modified TiO2nanoparticles are added to an aqueous solution containing Mn7+, the fluorescence intensity of both TiO2and ETP decreases. The evolution of the fluorescence intensity ratio (I1/I2) of TiO2and ETP is linearly related to the concentration of Mn7+. The sensitivity of fluorescence intensity to Mn7+concentration enables the design of dual fluorescence ratio solid particle sensors. The method proposed here is simple, accurate, efficient, and not affected by the environmental conditions.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
环境科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Huateng Zhang,Jing Su,Yuxin Lin,Haixiu Bai,Jiaxiang Liu,Hui Chen,Lupei Du,Lichuan Gu,Minyong Li
Chalcone refers to an aromatic ketone and an enone that constitutes the central core for various important biological compounds in drug discovery. Moreover, the firefly luciferase (Fluc) as the bioluminescent reporter has been widely used in life science research and high-throughput screening (HTS). However, Fluc might suffer from direct inhibition by HTS compounds resulting in the occurrence of “false positives.” In the current research, we discovered a series of chalcone compounds as Fluc inhibitors with favorable potency both in vitro and in vivo. Moreover, our compound 3i showed remarkable systemic inhibition in transgenic mice. Both enzymatic kinetics study and cocrystal structure demonstrated that compound 3i is competitive for substrate aminoluciferin, while noncompetitive for ATP. Besides, compound 3i exhibited excellent selectivity as a promising quenching agent in a simulated dual-luciferase reporter assay. We believed that our research would contribute to improving scientists’ awareness of the Fluc inhibitors, pay attention to the bias results, and even expand the utilization of bioluminescence in life science research.
研究领域:
药理学
·
分子生物学
·
生命科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2025
·
IF 7
Liang Yunyan,Zhou Xiaomeng,Liu Wenfeng,Shang Li
Rapid and effective differentiation of cancerous cells is essential for early diagnosis and treatment of tumors. Here, a multichannel sensor array is developed based on four surface-engineered gold-silver nanoclusters (AuAgNCs) to discriminate multiple cancerous and normal cells. Different binding affinities between various cells and AuAgNCs lead to changes in the intrinsic fluorescence of AuAgNCs as well as their peroxide-like activity. Based on the distinct response of dual fluorescence signals of four AuAgNCs, a multichannel sensor array is established, which is capable of accurately identifying both normal and cancerous cells, even cells of different subtypes and homologous cells from various individuals with high sensitivity. Moreover, this fluorescent clusterzyme-based sensor array enables quantitative detection of cancerous cells (e.g., HeLa cells) with good performance, which holds great potential as a promising platform for further cancer diagnosis.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Wen Tian,Songcheng Yu,Kaiyang Zhang,Tao Liu,Lihua Ding,Peng Zhang
The CRISPR/Cas12a system holds significant promise for biomedical applications. Nevertheless, the commonly used reporter, fluorophore–quencher-labeled substrates, is hindered by labor-intensive synthesis procedures and high costs, while also relying on a single-photon method and being vulnerable to environmental interference. Herein, a label-free dual-fluorescent functional nucleic acid (DFFNA) was engineered, comprising an aptamer domain for auramine O (AO) recognition and a dSpacer-integrated DNA duplex region for 5,6,7-trimethyl-1,8-naphthyridin-2-amine (ATMND) binding. The fluorescence of AO and ATMND can be enhanced and quenched, respectively, when bound to DFFNAs. The fluorescence intensity ratio between ATMND and AO increased significantly following the cleavage of DFFNAs by activated Cas12a, thus offering a universal, label-free, ratiometric fluorescent reporter for the CRISPR/Cas12a system. To explore the application of the DFFNA-based CRISPR/Cas12a system, a novel biosensor was developed to detect site-specific DNA methylation. It employs a methylation-sensitive restriction enzyme to recognize methylation sites, Cas12a for site-specific DNA identification and signal amplification, and DFFNAs to produce ratiometric fluorescence. The assay demonstrated remarkable specificity and sensitivity, with a limit of detection of 152 pM, due to the high resolution and trans -cleavage activity of Cas12a. The rationally designed and label-free DFFNAs enhance stability, increase flexibility, and reduce cost. The observable color change and smartphone imaging capability facilitate portable, point-of-care testing. Specifically, the biosensor demonstrated excellent specificity by differentiating colorectal cancer patients from healthy individuals. Consequently, this work presents a superior label-free and ratiometric fluorescent reporter for the CRISPR/Cas12a system, which offers a promising strategy for DNA methylation detection in clinical settings.
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产品关联
DOI
PubMed
高影响因子
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