JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Sha Li,Wei Wen,Yu Hao,Jun Zheng,Xingao Zhang
Hydrazine (N 2 H 4 ) and sulfur dioxide (SO 2 ) play important roles in environmental systems and organisms, but their excessive intake can lead to several diseases. Due to the lack of two-color probes capable of distinguishing between N 2 H 4 and SO 2 , we synthesized a novel dual-channal NIR fluorescent probe PI-CO-NH based on piperazine-substituted benzopyranone salts and coumarin carboxylic acids, which is a dual-function probe capable of detecting SO 2 and N 2 H 4 . When detecting N 2 H 4 , PI-CO-NH showed rapid fluorescence changes at 447 nm and 633 nm emission channels, and both blue and red light gradually increased with the increase of N 2 H 4 concentration. After adding SO 2 , the double bond on benzopyrrole was broken and the red fluorescence was quenched. The experimental results show that the probe has high selectivity and sensitivity, excellent stabilit and high cell permeability, can visualize N 2 H 4 and SO 2 in living cells, and has good mitochondrial targeting ability. More importantly, the ability of PI-CO-NH to detect N 2 H 4 in different soil samples and test strips was successfully verified. These experiments not only validate the applicability of the probe in complex environment, but also provide a strong basis for the future application of PI-CO-NH in environmental pollution detection.
研究领域:
生物化学
·
环境科学
·
医学检测
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DOI
高影响因子
INORGANIC CHEMISTRY
·
2021
·
IF 5.165
Xiaojun He,Feng Ding,Xiaoshuai Sun,Yujing Zheng,Wei Xu,Lisong Ye,Hong Chen,Jianliang Shen
Zinc/Zn(II) is an essential trace element for humans and acts as an important substance that maintains the normal growth, development, and metabolism of the body. Excess or deficient Zn(II) can cause abnormal metabolism in the human body, leading to a series of diseases. Moreover, biosystems have complex homeostasis systems, especially harsh pH (OH–) environments. Thus, investigating the variation in the levels of Zn(II) and OH– is extremely important in clinical, medical, and environmental testing. Nevertheless, the lack of practical and convenient fluorescence imaging tools limits the tracing of Zn(II) and OH– in biosystems. In this work, a well-designed dual-channel fluorescent signal response chemosensor (DACH-fhba) was assembled for selective sensing of Zn(II) and OH– in the biosystem using a fluorescence turn-on strategy. On encountering Zn(II), the chemosensor emitted a blue fluorescence signal (455 nm). Meanwhile, the bright green fluorescence signal (530 nm) increased with OH– addition simultaneously. With the blue/green dual fluorescence response of DACH-fhba, the sensor exhibited high stability and reversibility. Notably, the bioimaging revealed that DACH-fhba successfully tracked Zn(II) and OH– in live cells, larval zebrafish, and plants. Further results implied that DACH-fhba can be used to achieve visual detection of Zn(II) and OH– in organisms. Altogether, this work is conducive to the monitoring of Zn(II) and OH– in organisms and promotes the understanding of the function of Zn(II) and OH– in biosystems.
研究领域:
生物化学
·
医学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Jinli Fu,Shu Zhou,Sisi Tang,Xiaodan Wu,Pengfei Zhao,Kangling Tang,Yu Chen,Zhaoxia Yang,Zhaohui Zhang
The rapid and simple synthesis of highly efficient multi-emission luminescent metal-organic frameworks (LMOFs) with down/up-conversion fluorescence properties becomes critical in potential optical sensing. Here, we present a luminescent dual-ligand co-assisted strategy to one-pot prepare LMOFs for multiplex fluorescence sensing using carbon dots (CDs) and 2-methylimidazole (2-MIM) as dual-ligand to induce Zn 2+ on zeolitic imidazolate framework-8 surface (CDs/MIM-Zn). The CDs/MIM-Zn exhibited efficient fluorescence-enhanced dual channels triple-emission with Stokes and anti-Stokes type excitation profiles. With the assistance of Stokes and anti-Stokes type optical property of CDs and the intrinsic porosity of MOFs structure, a rapid and sensitive dual-channel detection excited at 370 nm and 790 nm was performed to fluorescence turn off-on detect metal ions and reduced glutathione in real blood samples with the satisfactory recoveries of 98.3–106.4%. The dual channels triple-emission strategy ensured multiplex detection of more analytes in parallel.
研究领域:
化学
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zixuan Teng,Huimin Shangguan,Longfei Liu,Shihu Zhang,Guanlin Li,Zishi Cheng,Fengpei Qi,Xingjiang Liu
The ubiquity of diverse material entities in environmental matrices renders the deployment of unifunctional fluorescent indicators inadequate. Consequently, this study introduces a ratiometric dual-emission fluorescent sensor ( Probe CP ), synthesized by conjugating phenothiazine coumarin to hydroxycoumarin through a piperazine linker for concurrent detection of HClO and H 2 S. Upon interaction with HClO, the phenothiazine unit's sulfur atom undergoes oxidation to sulfoxide, facilitating a shift from red to green fluorescence in a ratiometric manner. Concurrently, at the opposite terminus of Probe CP , 2,4-dinitroanisole serves as the reactive moiety for H 2 S recognition; it restores the blue emission characteristic of 7-hydroxycoumarin while maintaining the red fluorescence emanating from phenothiazine coumarin as an internal standard for ratio-based assessment. Exhibiting elevated specificity and sensitivity coupled with minimal detection thresholds (0.0506 μM for HClO and 1.7292 μM for H 2 S) alongside rapid equilibration periods (3 min for HClO and half an hour for H 2 S), this sensor was efficaciously employed in cellular environments and within zebrafish models as well as imaging applications pertaining to alcohol-induced hepatic injury in murine subjects.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
APPLIED OPTICS
·
2020
·Vol. 59
·期 3
·601-606
·
IF 1.961
Ting Liu,Wenqi Wang,He Ding,Zhiqun Liu,Suzhi Zhang,Dingrong Yi
In order to meet the needs of on-site, accurate, fast, and remote detection, we design a smartphone-based handheld dual-channel optical fiber fluorescence sensor (DOFFS), which is composed of a semiconductor laser for exciting fluorescence signals, a smartphone with a dual-bandpass filter for collecting fluorescence signals, a fiber coupler for transmitting light, and batteries for laser power supply. All the components are integrated into a 3D printed shell, on the side of which there are two fiber flanges used for fiber probe connection. The fluorescence signals of green and red quantum dots modified on the fiber probes can be captured by the smartphone camera and calculated by a self-developed Android application. The comparisons of single-channel and dual-channel fluorescence signals with pH show that the performance of the sensor is good. The proposed sensor not only can simultaneously detect dual-channel signals for fast detection needs, but it also is handheld with a small size of ${79} \times {57} \times {154}\;{{\rm mm}^3}$ and inner power supply, and the fiber probes can be easily replaced, supporting remote and on-site applications. It is a potential tool for many occasions in many fields.
研究领域:
生物医学工程
·
光学传感
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DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Lian Xu,Yan Xiao,Kun Yu,Hongshuo Pan,Jiayi Xu,Yiyun Guan,Mengke Wang,Xiangyu Xu,Hao Wang
The improper application of nonsteroidal anti-inflammatory drugs (NSAIDs) presents significant health hazards via vector food contamination. A critical limitation of these traditional existing approaches is their inability to concurrently discern and distinguish among diverse NSAIDs, presenting a notable gap in the analytical capabilities within this domain. Herein, a creative dual-channel fluorescence sensor array was developed for the rapid discrimination and determination of NSAIDs, utilizing complexes of cucurbit[8]uril (CB[8]) with three distinct modified poly(ethylenimines) (PEIs) to address this challenge. The array successfully differentiated and identified 19 NSAIDs with 97% accuracy at a concentration of 1 mM. In addition, it also achieved analyses of individual NSAIDs across a range of concentrations, NSAID mixtures, and impurities of aspirin using statistical analysis methods. More importantly, the approach effectively detected NSAIDs in complex matrices, such as milk and urine, demonstrating its potential for real-world applications.
研究领域:
分析化学
·
食品安全
·
药物检测
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yingqi Yuan,Lina Zhang,Jun Xu,Xinna Zhao,Ning Bi,Yongxin Li,Lei Jia
Contamination from tetracycline (TC) and copper ions (Cu 2+ ) poses a significant threat to aquatic ecosystems and human health. Developing an efficient platform to achieve ultrasensitive and on-site synchronous detection of TC and Cu 2+ is urgently needed. Herein, a dual-channel fluorescent nanoprobe (Eu-CDs@Eu-MOF) was elaborately designed based on europium ions (Eu 3+ ) decorated green-fluorescent carbon dots (CDs) cooperating with red-fluorescent Eu-MOF. This bifunctional nanoprobe exhibited specific response to Cu 2+ in the 275 nm channel, and special recognition ability for TC in the 385 nm channel due to the “antenna effect”. Ultrasensitive cross-interference-free detection of TC and Cu 2+ were realized with the LOD of 3.70 and 6.13 nM, respectively. Satisfactory results were achieved in various real samples detection. Moreover, smartphone-assisted portable paper- and hydrogel kit-based sensors were constructed for fast and on-site visual assay of TC and Cu 2+ . This study provided an effective method for ultrasensitive and simultaneous detection of TC and Cu 2+ , and offered some references for the construction of portable and multifunctional nanosensors for facile use in daily life.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Guoxing Yin,Yabing Gan,Ting Yu,Tingting Niu,Peng Yin,Haimin Chen,Youyu Zhang,Haitao Li,Shouzhuo Yao
A novel mitochondria-targeted fluorescent probe for sensing SO 2 derivatives was constructed by integrating phenanthroimidazole and cyanine moieties. Excited by different channels, this probe exhibited unique dual-channel fluorescence-enhanced emissions and colorimetric response to HSO 3 - quickly (less than 20 s). The sensing process was confirmed to undergo nucleophilic addition of HSO 3 - to C˭C double bond in the probe Mito-PTB via NMR and HRMS analyses. Furthermore, fluorescence co-localization studies demonstrated that the probe Mito-PTB was a mitochondria-targetable fluorescent probe for SO 2 derivatives with good cell membrane permeability . Thus, probe Mito-PTB has the potential applications to explore the role played by SO 2 derivatives in biology.
研究领域:
生物化学
·
细胞生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Sheng Chen,Wenji Wang,Mingming Yan,Qin Tu,Shu-Wei Chen,Tianbao Li,Mao-Sen Yuan,Jinyi Wang
The sensitive detection and quantification of heavy and transition metal ions are particularly important. Among them, mercury(II) (Hg 2+ ) and silver(I) (Ag + ) ions, as the most ubiquitous heavy metal ion pollutants, are difficult to discriminate in the same system because of their similar reactions and coordination abilities. Herein, we designed and synthesized a “turn-on” chemosensor, RBTP , which is an intergrant of rhodol and 2-hydroxy benzothiazole by introducing a benzothiazole unit to the ortho -position of the phenolic hydroxy of rhodol. RBTP showed a sensitive dual-channel fluorescence-enhanced response to Hg 2+ and Ag + at 595 and 520 nm with low detection limits of 0.27 and 0.45 μM, respectively. Studies of their sensing mechanism indicated that the Hg 2+ -induced orange fluorescence at 595 nm originates from an irreversible Hg 2+ -promoted oxadiazole forming reaction and the Ag + -induced green fluorescence at 520 nm originates from special molecular packing of the RBTP -Ag + complex. Furthermore, the potential of probe RBTP as practical biological imaging agents was further confirmed in HepG2 cells. Simultaneously, RBTP exhibited remarkable solid-state luminescence with an aggregation-induced emission (AIE) feature. The AIE mechanism was thoroughly explored using X-ray single-crystal structures and photophysical determinations.
研究领域:
分析化学
·
环境科学
·
生物成像
✓ 已验证
DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 14
·9529-9537
·
IF 3.9
Ping Yang,Tingfei Shi,Shuangquan Zhu,Kuiliang Li,Hong Zhao
In this study, we investigated the detection of latent fingerprints (LFPs) using green light- and near-infrared (NIR) light-induced up/down-conversion dual-channel composites. Upconverted yttrium aluminium garnet (YAG) was prepared using a citric acid-assisted sol–gel method. After loading rhodamine 6G (RhD-6) into mesoporous silica nanospheres (MSNs), the MSNs-RhD-6 composites were coated with the as-synthesised YAG via electrostatic adsorption using the layer-by-layer method, demonstrating reversible switching between yellow and green light waves under 525 nm green light or 980 nm laser excitation. To evaluate the effectiveness of YAG-MSNs-RhD-6 powder in criminal investigations, we conducted simulations for different fingerprint scenarios. The results indicated that even after prolonged aging (up to 20 days), exposure to water, or high-temperature baking, the fingerprints remained clearly visible in the images. The detection of LFPs on various substrate surfaces exhibited high contrast, with the details of the fingerprints easily observable even after appropriate magnification. This study opens a new path for green light- and near-infrared light-induced up/down-conversion dual-channel composites for optical applications.
研究领域:
材料科学
·
刑事科学技术
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jiaqi Wang,Yuan Fu,Dong Liang,Jiangbo Pan,YiChang Jing,Changhe Liao,Di Wang,Shuangying Wei,Chengyu Wang,Jian Li
Carbonized polymer dots (CPDs), a new nanofluorescent materials inheriting the advantages of CDs, have been widely studied for their excellent physicochemical stability and tunable fluorescence properties, and have extensive application in the fields of optoelectronic devices and environmental monitoring. In this study, nitrogen-doped CPDs (CMC-M-CPDs) were synthesized via hydrothermal reaction using carboxymethyl cellulose (CMC) and melamine (M), which allowed N doping to improve the luminescence of CPDs through cross-linking and reduction of the energy gap. Due to the excitation-dependent, the luminescence wavelength of CPDs can be tuned, so these CPDs exhibit two optimal emission centers in the fluorescence spectra, which originate from the carbon core and the surface, respectively. A significant quenching effect on the luminescence of CMC-M-CPDs at 457 nm and 515 nm was exhibited due to static quenching of Cr(VI). Therefore, dual-channel fluorescence detection of Cr(VI) can be achieved, allowing detection using both excitation wavelengths to be carried out concurrently and mutually verified in complex environments. This improves the accuracy of detection, with limits of detection (LOD) of 60.15 nM and 93.95 nM, respectively. The CMC-M-CPDs demonstrate excellent selectivity and anti-interference in the detection of Cr(VI), with a quenching response occurring within 1 s. This dual-channel fluorescent probe can also be applied for sensitive detection in tap water and Songhua River water. Furthermore, the fluorescence of quenched CPDs can be restored by adding ascorbic acid (AA), achieving a maximum recovery efficiency of 96.6 %.
研究领域:
环境监测
·
纳米材料
·
分析化学
·
荧光探针
·
光学传感
✓ 已验证
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Yu Chen,Yumin Wang,Xiang Hua Wu,Bo Liu,Jun Feng Zhang
Biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play important roles in various physiological and pathological processes. However, it is still a great challenge to selectively detect Cys/Hcy and GSH in biological samples due to the similarity of structures and re-activities of these biothiols. In this paper, a new dual-channel near infrared-7-nitro-1,2,3-benzoxadiazole ( NIR-NBD ) fluorescent probe has been developed for the discriminative detection of Cys/Hcy and GSH. The probe is composed of two functional parts: a near-infrared dye of dicyanoisophorone, as fluorophore precursor, and NBD as both fluorophore precursor and sensing group. It was observed that NIR-NBD probe displayed two different fluorescent signals in response to biothiols, green-red for Cys/Hcy and red for GSH. It also showed a near infrared emission (756 nm), low detection limit, fast response, high stability, selectivity and sensitivity. Furthermore, NIR-NBD was effectively applied for the discriminative detection of Cys/Hcy and GSH in HeLa cells .
研究领域:
化学
·
生物化学
·
细胞生物学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）