Analytical Methods
·
2014
·Vol. 6
·期 10
·3285-3290
·
IF 1.938
Yingtang Li,Chao Yang,Jinyan Ning,Yaling Yang
In this work, cloud point extraction (CPE) was developed for the preconcentration and separation of bisphenol A (BPA), bisphenol AF (BPAF) and tetrabromobisphenol A (TBBPA) prior to high-performance liquid chromatography (HPLC) analysis. Firstly, alcohol ethoxylate (AEO9) was applied as the extractant and octanol worked as the cloud point revulsant and synergic reagent for extraction, which decreased the cloud point temperature (CPT) of AEO9 from 75 °C to 30 °C and assisted the subsequent extraction. The parameters influencing the CPE efficiency, such as AEO9 concentration, octanol volume, pH value, extraction time and temperature were systematically evaluated. Under the optimum conditions, the linear range of BPA, BPAF and TBBPA was from 0.05 to 25 mg L−1. All correlation coefficients of the calibration curves were more than 0.9988. The relative standard deviations (RSD, n = 5) were 1.6–4.7%. The limits of detection (LOD) were in the range of 0.27–0.32 μg L−1 and the limits of quantification (LOQ) were in the range of 0.71–0.98 μg L−1. The proposed method was successfully applied to the extraction of BPA, BPAF and TBBPA in river water samples as a rapid, simple, and sensitive method.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 6
·5804-5813
·
IF 2.914
Meng Zhiyuan,Zhu Wentao,Wang Dezhen,Li Ruisheng,Jia Ming,Yan Sen,Yan Jin,Zhou Zhiqiang
The widespread application of bisphenols (BPs) in the industry has made them ubiquitous in the environment, causing potential environmental risks. Its unknown impacts on human being have received more and more attention. In this study, we have assessed the metabolic effects of perinatal exposure to bisphenol A (BPA) and its substitutes (bisphenol S (BPS), bisphenol F (BPF), and bisphenol AF (BPAF)) in female mice adolescent offspring and female mice adulthood offspring. 1 H NMR-based serum metabolomics showed that metabolic profiles were disturbed with BPA and its three substitutes exposure in female mice adolescent offspring and female mice adulthood offspring. In addition, age-related metabolic effects were found based on changes in serum endogenous metabolites and metabolic pathways. Specifically, metabolic pathway analysis showed that major disturbed metabolic pathways in female mice adulthood offspring compare with female mice adolescent offspring also changed significantly. With the increase of age of the female mice offspring, changes in the metabolic pathways became more obvious in the BPA treatment group. Conversely, partially disturbed metabolic pathways were restored in the BPS, BPF, and BPAF treatment groups. In conclusion, perinatal exposure to BPA and its three substitutes significantly interferes with metabolic profiles and metabolic pathways, and this metabolic effects were age-related. These results offer more detailed information about the age-related metabolic effects of perinatal exposure to BPA, BPS, BPF, and BPAF in female mice offspring and provide data for systematic evaluation of the health risk assessment of BPA and its substitutes.
研究领域:
环境毒理学
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代谢组学
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发育生物学
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生殖毒理学
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分子毒理学
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环境健康
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生命早期暴露
✓ 已验证
DOI
高影响因子
APPLIED CLAY SCIENCE
·
2022
·
IF 5.907
Fayang Guo,Dabao Li,Jeremy B. Fein,Jingcheng Xu,Yingwei Wang,Qiaoyun Huang,Xingmin Rong
Reactions occurring at silicate minerals play a significant role in controlling phenolic endocrine disrupting chemicals' bioavailability and mobility. However, the underlying molecular mechanisms for BPA (bisphenol A) and BPS (bisphenol S) onto montmorillonite (MMT) remain poorly understood. To decipher the molecular-level interfacial configuration and dominant reaction mechanism, batch experiments, X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), X-ray photoelectron spectroscopy (XPS) measurements, and density functional theory (DFT) calculations were performed. Our batch adsorption experiments showed that MMT exhibits a higher adsorption capacity for BPS than for BPA. The XRD analysis also suggested that BPA and BPS could enter the interlayer of MMT. FTIR and XPS results revealed that The Ca atoms and the oxygen atoms in the SiO 4 tetrahedra were involved in BPA and BPS adsorption process via non-covalent interactions. The electrostatic potential calculations suggested that the sulfonyl O site and the hydroxyl H site on the BPS molecule are more favorable for interacting with the MMT surface than the hydroxyl O and H sites of the BPA molecule. DFT calculations further confirmed that stronger binding of BPS than BPA onto the (001) surface and the interlayer of MMT. BPA tends to form Ca ion bridges through hydroxyl groups onto MMT. In contrast, BPS prefers to form Ca ion bridges onto MMT through its sulfonyl group and form hydrogen bonds via hydroxyl groups. This study provides new insights into the molecular-scale interactions that control BPA and BPS adsorption onto an important and environmentally-relevant clay mineral, and which affect the fate and behavior of BPA and BPS in soil systems.
研究领域:
环境科学
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分子生物学
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DOI
高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Jie Zhang,Tiehua Zhang,Tianzhu Guan,Ping Ruan,Dayong Ren,Weichang Dai,Hansong Yu,Tiezhu Li
A fluorescence polarization (FP) assay for the simultaneous determination of bisphenol A (BPA), bisphenol F (BPF), bisphenol A diglycidyl ether (BADGE) and bisphenol F diglycidyl ether (BFDGE) was developed. The method was based on the competition between bisphenols (BPs) and fluorescein-labeled dexamethasone derivative (Dex-fl) for mouse peroxisome proliferator-activated receptor α ligand binding domain (mPPARα-LBD). A recombinant soluble protein derivative mPPARα-LBD* was prepared, then in vitro binding of 4 BPs to mPPARα-LBD* was investigated. Fluorescence polarization assay showed that these compounds exhibited different binding potencies with mPPARα-LBD*. Additionally, molecular dynamics simulations were performed to further understand the mechanism of BPs binding affinity for mPPARα-LBD*. Docking results elucidated that the driving forces for the binding of BPs to mPPARα-LBD* were predominantly dependent on hydrophobic and hydrogen-bonding interactions. Comparison of the calculated binding energies vs. experimental binding affinities yielded a good correlation (R 2 = 0.7258). The proposed method has potential for multi-residue detection of BPA, BPF, BADGE, and BFDGE.
研究领域:
生物化学
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环境科学
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毒理学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Xijuan Tu,Chunping Du,Yuchang He,Ji Yang,Jiaxu Chen,Qian Jin,Lingfei Xie,Yuqing Zuo,Shaokang Huang,Wenbin Chen
The methodology of sugaring out–assisted liquid–liquid extraction (SULLE) coupled with high-performance liquid chromatography–fluorescence detection was devised for quantifying bisphenol A (BPA) and bisphenol B (BPB) in beeswax. The effectiveness of SULLE was methodically explored and proved superior to the salting out–assisted liquid–liquid extraction approach for beeswax sample preparation. The analytical performance underwent comprehensive validation, revealing detection limits of 10 μg/kg for BPA and 20 μg/kg for BPB. The method developed was employed to analyse commercial beeswax (n = 15), beeswax foundation (n = 15) and wild-build comb wax (n = 26) samples. The analysis revealed BPA presence in four commercial beeswax samples and three beeswax foundation samples, with the highest detected residue content being 88 ± 7 μg/kg. For BPB, two beeswax foundation samples were positive, with concentrations below the limits of quantification and 85 ± 4 μg/kg, respectively. No bisphenols were detected in wild-build comb wax. Furthermore, the bisphenol removal efficacy of two recycling methods—boiling in water and methanol extraction—was assessed. The findings indicated that after four recycling cycles using water boiling, 9.6% of BPA and 29.2% of BPB remained in the beeswax. Whereas methanol extraction resulted in approximately 7% residual after one recycling process. A long-term study over 210 days revealed the slow degradation of bisphenols in comb beeswax. This degradation fitted well with a first-order model, indicating half-lives (DT 50 ) of 139 days for BPA and 151 days for BPB, respectively. This research provides the first report on bisphenol contamination in beeswax. The low removal rate during the recycling process and the gradual degradation in beeswax underscore the significance of bisphenol contamination and migration in bee hives along with their potential risk to pollinators warranting concern. Furthermore, the developed SULLE method shows promise in preparing beeswax samples to analyse other analytes.
研究领域:
环境科学
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分析化学
·
生态学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2015
·
IF 1.03
Shuangqiao Ju,Yaling Yang,Mousheng Liu
A method for magnetic solid-phase extraction was developed for the preconcentration of bisphenol A, bisphenol AF, tetrabromobisphenol A, and 4-tert-octylphenol from food containers and packaging materials. Cetyltrimethylammonium bromide was added to a solution of magnetic nanoparticles to enhance adsorption of the analytes prior to high-performance liquid chromatography. The effects of the amount of surfactant, the amount of magnetic nanoparticles, the pH, the adsorption time, the desorption solution, and the reuse of the extractant were optimized. The linear dynamic ranges were from 0.05 to 25 milligrams per liter. The limits of detection were between 1.21 and 2.48 micrograms per liter, the limits of quantification were from 4.03 to 8.27 micrograms per liter, and the relative standard deviations were between 2.2 and 4.1 percent. This magnetic solid-phase extraction approach was successfully employed for the analysis of plastics with recoveries from 88.0 to 101.1 percent and relative standard deviations between 2.3 and 5.4 percent.
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 8
·1755-1764
·
IF 2.557
Sheng-Xiang Gong,Xiao-Li Wang,Wei Liu,Ming-Lin Wang,Xia Wang,Zheng-Wu Wang,Ru-Song Zhao
Aminosilanized magnetic carbon microspheres as a novel adsorbent were designed and fabricated. The adsorbent was used for the magnetic solid-phase extraction of bisphenols at trace levels from environmental water samples before liquid chromatography with tandem mass spectrometry analysis. The structure, surface, and magnetic behavior of the as-prepared aminosilanized magnetic carbon microspheres were characterized by elemental analysis, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, powder X-ray diffraction, and vibrating sample magnetometry. The effects of the experimental parameters were investigated by the Plackett–Burman design, and then the parameters that were significant to the extraction efficiencies were optimized through a response surface methodology. The aminosilanized magnetic carbon microspheres exhibited high adsorption efficiency and selectivity for bisphenols. Under optimal conditions, low limits of detection (0.011–2.22 ng/L), and a wide linear range (2–3 orders of magnitude), good repeatability (4.7–7.8%, n = 5), and reproducibility (6.0–8.3%, n = 3) were achieved. The results demonstrate that the novel adsorbent possesses great potentials in the determination of trace levels of bisphenols in environmental water samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Xincong Su,Li Kai,Xiaowen Han,Rongzhi Wang,Xiao Yang,Xuedong Wang,Jin Yan,Qiuhui Qian,Zejun Wang,Huili Wang
Bisphenol S (BPS) and Bisphenol F (BPF), as alternatives to bisphenol A (BPA), are recognized for their endocrine-disrupting properties, but their combined immune toxicity mechanisms remain poorly understood. This study systematically evaluates the individual and joint immune toxicity effects of BPS and BPF through ADMET predictions, transgenic zebrafish models, and molecular docking analyses. The results indicate that equal effect concentration BPS and BPF act through distinct immune pathways: BPS primarily targets macrophages to mediate immune responses, while BPF significantly stimulates neutrophil proliferation and induces a stronger inflammatory response through chemokine signaling. Molecular docking studies show that BPF binds more stably to pro-apoptotic protein Mapk8 and oxidative stress-related protein Hsp90aa1, leading to significantly higher levels of apoptosis and reactive oxygen species (ROS) compared to BPS. The similarity of modes of action (MOA)between BPS and BPF based on relevant immune indicators calculated and experimentally is about 0.3; this quantitative result also proves that modes of action differ widely. Nonetheless, most of the indicators showed superimposed effects in the combined experiments, and it is noteworthy that the oxidative stress indicators (SOD, MDA) showed synergistic effects, suggesting that BPS and BPF, which have very different modes of action, are able to be risk assessed using an additive model with respect to immunity, but may exhibit synergistic risks with respect to oxidative stress. This research demonstrates that BPS and BPF induce immune toxicity via different molecular targets and pathways and highlights the need to account for their synergistic effects in risk assessments. These findings provide important insights into the immune toxicity mechanisms of BPA substitutes and the potential risks of combined exposures.
研究领域:
环境科学
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毒理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2014
·Vol. 25
·期 8
·1512-1519
·
IF 1.253
Li Yingtang,Yang Guorong,Zhao Jiao,Yang Yaling
In this study, a vortex-assisted three-phase hollow fiber liquid-phase microextraction (VA-HF-LPME) coupling with high performance liquid chromatography (HPLC) method was developed for determination of three synthetic endocrine disrupting compounds[EDCs, bisphenol-A (BPA), bisphenol-AF (BPAF) and tetrabromobisphenol-A (TBBPA)] in milk samples. Vortex provided effective and mild mixing of the sample solution and increased the contact between analytes and boundary layers of the hollow fibre, thereby enhancing mass transfer rate and leading to high extraction efficiency of the target analytes. The influencing parameters of VA-HF-LPME sample preparation method, such as organic solvents (acceptor phase), pH of sample solution (donor phase), sample volume, concentration of NaOH, extraction time, and ionic strength were systematically optimized. The instrumental calibration curves of BPA, BPAF and TBBPA show good linear relations (R² > 0.9988) in the concentration range of 0.5-200, 0.5-200 and 1.0-250 µg L-1, respectively. The relative standard deviations (RSD, n = 5) were 1.3-3.7%. The limits of detection (LOD, S/N = 3) were in the range of 0.16-0.35 µg L-1 and the limits of quantification (LOQ, S/N = 10) were in the range of 0.51-1.12 µg L-1. The proposed method was successfully applied to the extraction of EDCs in milk samples.
研究领域:
分析化学
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环境科学
·
食品安全
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产品关联
DOI
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2025
·Vol. 197
·期 8
·1-13
·
IF 3
Luo Huili,Pan Xiaopeng,Yang Yixuan,Huang Yilu,Xiang Yuhang,Li Anxin,Zhao Jia
Bisphenol analogues (BPs), the endocrine-disrupting compounds, have been detected in water, sediment, and even agricultural products. Irrigation is the main way for material migration, and it has been suggested as one of the sources of BPs. But the attention to irrigation water and sediment, and further to the ecological risks, is rare. In a hilly irrigation area in southern China, the partition and accumulation, contribution sources, and potential ecological risk of four bisphenols (BPA, bisphenol S, F, and AF) in the irrigation canal were investigated in the spring of 2023 and 2024. All four BPs were detected in the irrigation water and sediment. BPF was the dominant of 19.6–60.9 ng/L in water, while BPA was still the prominent compound (46.2–54.4% of ∑BPs) in sediment. BPs in the irrigation canal were related to the direct discharge of aquaculture, domestic, and planting drainage. Although low risks for each compound, the comprehensive risk (∑RQ) of sediments went into medium risk. BPF and BPA were the main risk contributors. BPs in sediments increased steadily during the investigation. The special accumulative point was where slow flow maintained, especially caused by the hilly change. This comprehensive study of BPs in hilly irrigation canals should be valuable for reference in similar areas. And it will be beneficial for environmental protection and production safety.
研究领域:
环境科学
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水污染研究
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生态毒理学
·
农业环境管理
·
内分泌干扰物
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 36
·16107-16118
·
IF 2.7
Zhoufeng Wang,Bolin Wang,Yingying Liu,Xiubo Long,Wenlong Yao
A series of linear block polymers and random polymers were synthesized by interfacial polycondensation using two bisphenols with similar structures, bisphenol S (BPS) and bisphenol A (BPA), and terephthaloyl chloride and isophthaloyl chloride. Random polymers and block polymers with the expected topology were structurally characterized by 1H NMR, FTIR, GPC, WAXD, and Raman spectroscopy. The results demonstrated that there was no significant difference in the thermal stability of the block and random polyarylates, which was due to the fact that the covalent bonds of the linked monomers did not change substantially, and the bond energies were the same. However, due to the alteration of the molecular sequence structure, the block polymer exhibited a lower glass transition temperature, a pronounced melting peak, and better solubility. With physical cross-linking points consisting of microcrystalline phases formed by the relatively regular molecular chain structure, the block polyarylates exhibited a higher modulus of elasticity than the random polyarylates. This illustrated that it is possible to prepare new functional polyarylates without changing the monomer composition, but only by manipulating the reaction process.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 3
·951-959
·
IF 4.58
Zhang Yuhuan,Wang Yanru,Zhu Wenxin,Wang Jing,Yue Xiaoyue,Liu Wei,Zhang Daohong,Wang Jianlong
The capability of multi-level logic operations in biological diagnosis is far from being fully realized. In particular, the simplification of logic judgments to realize rapid, on-site complex diagnoses is still a key issue in bioelectronics. The authors here describe the construction of a colorimetric multilevel DNA circuit that incorporates two IMPLY gates and a sample extraction technology (SET) with anti-BPA aptamer as a co-recognizing element and the color of gold nanoparticles as the output signal. The circuit can perform multi-bioanalysis of the plasticizers and hormone mimetics bisphenol A (BPA) and bisphenol S (BPS). The assay is based on the finding that the aptamer against BPA can recognize both BPA and BPS. This DNA logic detection system is simple, fast, sensitive and selective because of the utilization of a co-recognition element which decreased input numbers. This, in turn, cascades multiplex Boolean logic gates and simplifies the diagnostic challenge. Both BPA and BPS can be determined by photometry at 620 nm, the limit of detection being 1.5 ng⋅mL −1 for BPA and 1.3 ng⋅mL −1 for BPS. Application to (spiked) water samples shows good analytical performance in terms of recovery (95.4 to 106.8%), linear range (4.4 to 66 ng⋅mL −1 for BPA, 2.5 to 75 ng⋅mL −1 for BPS) and correlation coefficients (>0.98). In our perception, the method represents a simple route for the fabrication of colorimetric multi-level DNA circuits. It may shorten the gap between bioelectronic logic circuits and high throughput real sample analysis.
Illustration of sample extraction treatments (SET) of bisphenol A (BPA) and bisphenol S (BPS) and the three-input IMPLY1-IMPLY2 logic operation with BPA, BPS and anti-BPA aptamer as inputs.
研究领域:
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）