CHEMOSPHERE
·
2023
·
IF 8.8
Xiaorong Wu,Shiqi Li,Meijia Zhang,Shengjun Bai,Yangyue Ni,Qiaoqiao Xu,Yun Fan,Chuncheng Lu,Zhipeng Xu,Chenbo Ji,Guizhen Du,Yufeng Qin
Bisphenol AP (BPAP), a structural analog of bisphenol A (BPA), has been widely detected in environment and biota. BPAP was reported to interfere with hormone and metabolism, while limited data were available about its effects on neurobehavior, especially exposure to it during early-life time. A mouse model of early-life BPAP exposure was established to evaluate the long-term neurobehaviors in offspring . Collectively, early-life BPAP exposure caused anxiety-like behaviors and impaired learning and memory in adult offspring. Through brain bulk RNA-sequencing (RNA-seq), we found differential expressed genes were enriched in pathways related to behaviors and neurodevelopment, which were consistent with the observed phenotype. Besides, single-nucleus RNA-sequencing (snRNA-seq) showed BPAP exposure altered the transcriptome of microglia in hippocampus. Mechanistically, BPAP exposure induced inflammations in hippocampus through upregulating Iba-1 and activating the microglia. In addition, we observed that BPAP exposure could activate peripheral immunity and promote proportion of macrophages and activation of dendritic cells in the offspring. In conclusion, early-life exposure to BPAP impaired neurobehaviors in adult offspring accompanied with excessive activation of hippocampal microglia. Our findings provide new clues to the underlying mechanisms of BPAP's neurotoxic effects and therefore more cautions should be taken about BPAP.
研究领域:
毒理学
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神经科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2021
·
IF 2.826
Huimin Li,Shaofan Guo,Lu Wang
This paper reports a new voltammetric method for detection of bisphenol AP (BPAP) with high sensitivity, which is based on a new electrochemical sensor from a three-component composite-modified glassy carbon electrode. The composite was characterised by various surface, structural and electrochemical analysis techniques, such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), Infrared Spectrum (IR), X-ray diffraction (XRD), Energy Dispersive X-Ray Spectroscopy (EDX) and Cyclic voltammograms (CV). The sensor had a well voltammetric response to BPAP, and the linear response ranges from 0.005 μM ~7 μM with the detection limits of 2 nM (S/N = 3). Finally, this system was utilised for determining BPAP in industrial wastewater using the standard addition method.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Tian-Zhu GUAN,Ning LI,Ya GAO,Ya-Jun GAO,Ming YANG,Qiong LI,Li-Xia XIAO
The hazardous effects of bisphenol compounds on organisms have attracted wide attention. Hence, elaborating of the specific binding mode of emerging contaminants to macromolecules provides a pivotal insight into the interaction mechanism. In the present study, the structure changes of bovine serum albumin (BSA) upon binding with bisphenol AP (BPAP) were explored via multi-spectroscopies under simulated physiological conditions. Stern-Volmer calculation showed BPAP quenched the endogenous fluorescence of BSA by static quenching. Thermodynamic results with respect to temperatures indicate that the van der Waals force and hydrogen bond interactions play a pivotal role in BSA-BPAP complex formation. Circular dichroism and Fourier transform infrared spectroscopies also quantitatively confirm the secondary structural changes of the complex. Explicit topological analysis was theoretically conducted by the wavefunction analysis method. Electrostatic potential map pinpointed the global surface minimum and maximum of BPAP, which correspond to −26.86 and 52.50 kcal mol −1 around oxygen atom and hydrogen atom, respectively. To acquire clearer perspective, local orbital localizer and electron localization function were calculated to visually represent the electron delocalization in BPAP. More specifically, molecular docking indicated that BPAP was located in the hydrophobic cavity of BSA. Herein, the experimental and theoretical results reveal BPAP and BSA interaction mechanism in detail. These insights have great value in warning the toxicity assessment of BPAP on BSA in humans and wildlife.
研究领域:
生物化学
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环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
Sustainable Materials and Technologies
·
2023
·
IF 9.6
Jinghan Guo,Yalin Wu,Yanhui Li,Shuangying Li,Zhi Li,Jingwen Niu,Chunmao Chen,Qingxiang Zhou
A creative electrochemical probe utilizing molecularly imprinted modified magnetic carbon dots was designed and fabricated. Carbon dots are a new kind of green, pollution-free, cheap and easy to obtain nanomaterials . The combination of Fe 3 O 4 and carbon dots improved the defect of ferric oxide being easy to aggregate, and their co-modification significantly enhanced the electrochemical activity of the electrode. Cyclic voltammetry and differential pulse voltammetry were executed to evaluate the electrochemical behavior of the co-modified electrode surface and performance of the probe. The constructed probe exhibited excellent selectivity and sensitivity for determining bisphenol AP in plastic products. The introduction of molecularly imprinted polymers endowed the sensor excellent selectivity, ensuring specific recognition of bisphenol AP. The fabricated sensor demonstrated a good two-stage linear range of 0.07–0.3 μM and 0.3–10 μM, and the detection limit was 0.027 μM. The constructed probe was validated to detect bisphenol AP in self-heating hot pot, instant noodle bucket and disposable plastic cup, and satisfactory results were achieved. These results indicated that the fabricated sensor was a cheap, rapid, and selective sensing-platform for detecting bisphenol AP, and could be employed as a reliable routine tool.
研究领域:
分析化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Yuanyuan Song,Xing Zhang,Binquan Wang,Xiaoxiao Luo,Ke Zhang,Xiaoyan Zhang,Qian Wu,Mingkuan Sun
Bisphenol AP (BPAP), an environmental endocrine disruptor, may cause neurodevelopmental disorders affecting human health. Studies have shown that BPAP impacts hormone synthesis and metabolism, causes social behavior abnormalities, and induces anxiety-like behavioral impairments in mice. However, evidence for the neurobehavioral effects of BPAP is still lacking. Here, we examined the toxic effects of BPAP on neurodevelopment using a Drosophila model. We assessed the role of BPAP exposure in autism-like behavior and explored the underlying mechanisms. Our findings indicated that BPAP exposure reduced pupation and eclosion rates and delayed growth in Drosophila . Furthermore, BPAP exposure caused autism-like behaviors, characterized by increased grooming times and aberrant social interactions, along with abnormalities in locomotor activity, as well as learning and memory ability. Mechanistically, we found that BPAP decreases the number of neuroblasts (NBs) and mature intermediate neural progenitors (INPs) in the 3rd larval brain, impairing axon guidance in the mushroom body of the adult Drosophila brain. Additionally, our transcriptome analysis revealed that BPAP exposure alters the expression of neurodevelopment-related genes ( Nplp3 , sand , lush , and orco ) and affects the estrogen signaling pathway ( Hsp70Ab , Hsp70Bc , Hsp70Ba , and Hsp70Bb ). These changes potentially explain the BPAP-induced autism-like behavior in Drosophila .
研究领域:
神经生物学
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环境科学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 25
·50585
·
IF 3.125
Pengcheng Li,Dawei Yan,Lin Luo,Wanli Tang,Haohao Ren,Yonggang Yan
A series of poly(arylene ether nitrile) copolymers (PENAPs) were synthesized with bisphenol A (BP-A), bisphenol AP (BP-AP) and 2,6-Dichlorobenzonitrile (DCBN) via a nucleophilic substitution polycondensation reaction. FTIR and 1 H-NMR were used to confirm the structure of PENAPs. Glass transition temperature ( T g ) of PENAPs determined by differential scanning calorimetry (DSC) ranged from 154.2 to 200.8°C. The 5% weight lost temperature ( T 5% ) of PENAPs were 418.9–447.7°C. The tensile and DMA test indicated that PENAPs possessed excellent mechanical properties with tensile strength more than 92.8 MPa and storage modulus more than 1.0 GPa at about 150°C. The melt flowability was measured by rheology properties testing ranging from 80 to 1639 GPa at 290°C and under shear frequency 100 Hz, which indicated the copolymers had good flowability and thermal stability. Additionally, PENAPs could be dissolved in many solutions, which meant PENAPs had good solubility and can be processed by solution method.
研究领域:
高分子化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）