CHEMOSPHERE
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2024
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IF 8.1
Xijuan Tu,Chunping Du,Yuchang He,Ji Yang,Jiaxu Chen,Qian Jin,Lingfei Xie,Yuqing Zuo,Shaokang Huang,Wenbin Chen
The methodology of sugaring out–assisted liquid–liquid extraction (SULLE) coupled with high-performance liquid chromatography–fluorescence detection was devised for quantifying bisphenol A (BPA) and bisphenol B (BPB) in beeswax. The effectiveness of SULLE was methodically explored and proved superior to the salting out–assisted liquid–liquid extraction approach for beeswax sample preparation. The analytical performance underwent comprehensive validation, revealing detection limits of 10 μg/kg for BPA and 20 μg/kg for BPB. The method developed was employed to analyse commercial beeswax (n = 15), beeswax foundation (n = 15) and wild-build comb wax (n = 26) samples. The analysis revealed BPA presence in four commercial beeswax samples and three beeswax foundation samples, with the highest detected residue content being 88 ± 7 μg/kg. For BPB, two beeswax foundation samples were positive, with concentrations below the limits of quantification and 85 ± 4 μg/kg, respectively. No bisphenols were detected in wild-build comb wax. Furthermore, the bisphenol removal efficacy of two recycling methods—boiling in water and methanol extraction—was assessed. The findings indicated that after four recycling cycles using water boiling, 9.6% of BPA and 29.2% of BPB remained in the beeswax. Whereas methanol extraction resulted in approximately 7% residual after one recycling process. A long-term study over 210 days revealed the slow degradation of bisphenols in comb beeswax. This degradation fitted well with a first-order model, indicating half-lives (DT 50 ) of 139 days for BPA and 151 days for BPB, respectively. This research provides the first report on bisphenol contamination in beeswax. The low removal rate during the recycling process and the gradual degradation in beeswax underscore the significance of bisphenol contamination and migration in bee hives along with their potential risk to pollinators warranting concern. Furthermore, the developed SULLE method shows promise in preparing beeswax samples to analyse other analytes.
研究领域:
环境科学
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分析化学
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生态学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 38
·e54439
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IF 3
Zhoufeng Wang,Junwei Hu,Bolin Wang,Yingying Liu,Xiubo Long
A series of novel aromatic copolyesters derived from bisphenol B (BPB) and phenol red (PR) were synthesized by interfacial polymerization. The number- and weight-average molecular weight ( M n and M w ) of the obtained amorphous aromatic copolyesters ranged from 1.06–1.80 × 10 4 to 2.01–6.17 × 10 4 g/mol, respectively. Density functional theory calculations showed that the reactivity of BPB was similar to that of bisphenol A. The reaction activation energy of BPB with terephthaloyl and isophthaloyl chloride (TPC and IPC) was 8.23 kcal/mol, and PR with IPC and TPC was 59.30 kcal/mol. Under the influence of the ethyl side group of BPB and the pendant group of PR, the solubility of the aromatic copolymer was improved, and the glass transition temperature ( T g ) was 125.1–195.3°C, while T max could still be maintained above 525.72°C. The optical transmittance of the copolyesters at 450 nm ( T 450 ) was 69.29%–99.41%, and the tensile strength and Young's modulus were in the range of 46.79–58.59 MPa and 1.39–1.86 GPa respectively. All aromatic copolyesters exhibit good mechanical and processability properties and have great application potential as high-performance plastics.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 19
·9507
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IF 4.9
Sen Zhao,Heliang Ni,Yuan Xiao,Jing Du,Yudong Han,Wenying Wang,Shuang Tang,Mingxi Yu
Accumulating evidence indicates that bisphenol A (BPA) analogs, including bisphenol B (BPB) and bisphenol S (BPS), disrupt testicular function and contribute to male reproductive dysfunction (MRD). However, whether BPA analogs are involved in MRD among middle-aged men remains inconclusive. Therefore, we selected cryptorchidism, erectile dysfunction, premature ejaculation, and testicular tumors as representative MRD conditions in middle-aged individuals, aiming to explore the molecular mechanisms that may be disrupted by bisphenols (BPs). By using GeneCards, STRING and Cytoscape, TP53, AKT1, and MYC were pinpointed as core targets associated with MRD. Enrichment analysis suggested that BPs may induce MRD by disrupting steroidogenesis. UPLC-MS/MS analysis showed that both BPB and BPS exhibit specific accumulation in the testes. Following 20-day exposure to 0.3 or 0.6 mg/kg body weight/day BPB or BPS, testosterone levels and the expression of hub genes were decreased. The molecular docking results demonstrated that both BPB and BPS can directly bind to members of the cytochrome P450 family, potentially interfering with sex hormone biosynthesis. Our study identified the targets and mechanisms through which BPB and BPS induce MRD in middle-aged males, thereby providing insights for the safety assessment of BPs.
研究领域:
生殖生物学
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环境毒理学
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分子内分泌学
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药代动力学
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生物标志物研究
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DOI
PubMed
高影响因子
MEASUREMENT
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2019
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IF 2.791
Yi Xing,Guanlan Wu,Ying Ma,Yangyang Yu,Xing Yuan,Xiaolin Zhu
A simple and highly sensitive electrochemical sensor for the determination of bisphenol B (BPB) based on a glassy carbon electrode (GCE) modified with Prussian blue (PB) and carboxylated multi-walled carbon nanotubes (MWNTs-COOH) was constructed. The PB/MWCNTs-COOH/GCE was characterized by emission scanning electron microscopy, X-ray diffraction, electrochemical impedance spectroscopy, and cyclic voltammetry. Due to the synergistic effect, the PB/MWCNTs-COOH/GCE remarkably enhanced the oxidation of BPB, which improved the anodic peak current of BPB significantly. Under the optimum conditions, the linear response range of BPB was between 5 × 10 −8 and 1.75 × 10 −4 M with the detection limit (S/N = 3) of 5 × 10 −10 M. Additionally, the PB/MWCNTs-COOH/GCE revealed excellent stability, repeatability and reproducibility. Moreover, it was successfully applied in the determination of BPB in real samples. This study provided a sensitive and simple sensor for the quantitative detection of BPB in the environment.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
Food Analytical Methods
·
2018
·Vol. 12
·期 3
·705-711
·
IF 2.245
Tu Xijuan,Wu Siyuan,Liu Weiyi,Gao Zhaosheng,Huang Shaokang,Chen Wenbin
An analytical method based on sugaring-out assisted liquid-liquid extraction (SULLE) and HPLC-fluorescence detection has been developed for the determination of bisphenol A (BPA) and bisphenol B (BPB) in royal jelly. Experimental parameters of SULLE including the extraction solvent, amount of phase separation agent, initial concentration of acetonitrile (ACN), and sample size were investigated. The optimal SULLE procedure involves 0.3 g sample extracted by 2 mL ACN-water mixture (50%, v / v ), followed by phase separation with the addition of 0.4 g glucose. The obtained extract was injected into the reversed-phase HPLC system without clean-up step. The chromatography separation of BPA and BPB from royal jelly matrix was achieved in the mobile phase composed of 40% ACN and 60% water with isocratic elution at 1.0 mL/min. Limits of quantification for BPA and BPB were 40 μg/kg and 45 μg/kg, respectively, and limits of detection for BPA and BPB were 16 μg/kg and 18 μg/kg, respectively. Average recoveries of BPA and BPB at three spiked levels were in the range of 88.32~93.59% and 95.14~97.48%, respectively. Precision expressed as relative standard deviations (RSDs) in the interday and intraday analysis were all lower than 5%. The developed method was applied to the analysis of ten commercial and eight raw royal jelly samples. Results indicated that none of BPA and BPB were detected in these 18 samples. The proposed method is simple, fast, and sensitive, and could be used for the monitoring of BPA and BPB contamination in royal jelly.
研究领域:
分析化学
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食品安全
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DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
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2024
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IF 4.4
Xuening Ma,Qianchao Shao,Shuxian Huang,Weigao Zhang,Hu Liu,Xu Jiayi,Xunan Zhao,Peiqi Li,Da Shao,YuanQing Bu,Dan Weng
The increasing incidence of cancer underscore the necessity of investigating contributors such as endocrine-disrupting chemicals (EDCs), including bisphenol A (BPA). Although BPA's risks are well-documented, comprehensive studies on its substitutes, such as bisphenol B (BPB), are limited. Dysregulated lipid metabolism is a hallmark of cancer progression. Our previous work demonstrated that BPA and bisphenol S (BPS) disrupt lipid metabolism via the peroxisome proliferator-activated receptor γ (PPARγ) pathway. We hence hypothesized that BPB might similarly perturb lipid metabolism and promote tumor growth. BPB's impact on lipid metabolism was investigated in vitro and in vivo using B16 melanoma cancer cells. Our findings indicate BPB exposure significantly increased lipid metabolism in B16 cells, enhancing cell proliferation and migration, and promoting tumor development in mice. Utilizing siRNA transfection or chemical inhibitor, we found that stearoyl-CoA desaturase-1 (SCD1), a key enzyme in lipid synthesis pathway, was required for BPB-induced lipid accumulation and cancer cell migration. Docking analysis revealed BPB may activate gene expression related to lipid metabolism and angiogenesis by interacting with PPARγ and hypoxia-inducible factor-1α (HIF-1α). This study illuminates BPB's potential role in advancing melanoma through lipid metabolism manipulation, highlighting the need for further research into the safety of BPA substitutes and their impact on cancer development.
研究领域:
肿瘤学
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分子生物学
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环境健康
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细胞生物学
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DOI
PubMed
高影响因子
Frontiers in Cell and Developmental Biology
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2020
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IF 5.186
Zhang Shou-Xin,Ding Zhi-Ming,Ahmad Muhammad Jamil,Wang Yong-Sheng,Duan Ze-Qun,Miao Yi-Liang,Xiong Jia-Jun,Huo Li-Jun
Bisphenol B (BPB), a substitute of bisphenol A (BPA), is widely used in the polycarbonate plastic and resins production. However, BPB proved to be not a safe alternative to BPA, and as an endocrine disruptor, it can harm the health of humans and animals. In the present study, we explored the effects of BPB on mouse oocyte meiotic maturation in vitro. We found that 150 μM of BPB significantly compromised the first polar body extrusion (PBE) and disrupted the cell cycle progression with meiotic arrest. The spindle assembly and chromosome alignment were disordered after BPB exposure, which was further demonstrated by the aberrant localization of p-MAPK. Also, BPB exposure increased the acetylation levels of α-tubulin. As a result, the spindle assemble checkpoint (SAC) was continuously provoked, contributing to meiotic arrest. We further demonstrated that BPB severely induced DNA damage, but the ROS and ATP production were not altered. Furthermore, the epigenetic modifications were changed after BPB exposure, as indicated by increased K3K9me3 and H3K27me3 levels. Besides, the pattern of estrogen receptor α (ERα) dynamics was disrupted with a mass gathering on the spindle in BPB-exposed oocytes. Our collective results indicated that exposure to BPB compromised meiotic maturation and damaged oocyte quality by affecting spindle assembly and chromosome alignment, acetylation of α-tubulin, DNA damage, epigenetic modifications, and ERα dynamics in mouse oocytes.
研究领域:
生殖生物学
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毒理学
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细胞生物学
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DOI
PubMed
高影响因子
Nano Today
·
2024
·
IF 13.2
Ke Zhu,Wenlei Qin,Yuwen Chen,Xiaoyin Liang,Haoran Xin,Zhihan Huang,Hector F. Garces,Yaping Gan,Mika Sillanpää,Rongliang Qiu,Guoqing Guan,Kai Yan
Constructing the coordination environment of single-atom catalysts (SACs) can be an attractive technical strategy to regulate the generation of reactive oxygen species for Fenton-like reactions. Herein, we have constructed an asymmetric coordination of Fe single-atom catalyst (Fe SA -NS-PCNS) with abundant N-Fe-S bridge (Fe SA -N 3 S 1 ) for robust Fenton-like reactions. 82.5 % of singlet oxygen ( 1 O 2 ) selectivity and high turnover frequency of bisphenol A degradation (0.568 min −1 ) were achieved at mild conditions. Experimental works and theoretical analyses illustrated that S doping breaks the inert environment of the original N-Fe-N symmetric coordination equilibrium and modulates the electron density of the atomic Fe center, which is beneficial for boosting PMS adsorption and reducing the energy barriers of vital *OH and *O intermediates. The coupling between the Fe SA -N 3 S 1 interface and peroxymonosulfate molecule boosts in-situ electron transfer through the N-Fe-S bridge, which induces more electron flow from the low valence Fe to OH* on the surface of Fe-*O-H, forming a high yield of 1 O 2 . Moreover, we designed the Fenton-like reactions by Fe SA -NS-PCNS membrane reactor for an efficient contaminant removal rate of over 90 % even after 11 cycles. This work provides a novel perspective on developing SACs with asymmetric coordination to regulate reactive oxygen species for the treatment of organic contaminants in water bodies.
研究领域:
催化
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环境化学
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材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Guijiao Zhao,Chenhuan Wang,Min Kang,Lin Hao,Weihua Liu,Zhi Wang,Xiaodong Shi,Qiuhua Wu
Developing effective methods for highly sensitive detection of phenolic endocrine disruptors (EDCs) is especially urgent. Herein, a magnetic hydroxyl-functional porous organic polymer (M-FH-POP) was facilely synthesized by green diazo-couple reaction using basic fuchsin and hesperetin as monomer for the first time. M-FH-POP delivered superior adsorption performance for phenolic EDCs. The adsorption mechanism was hydrogen bonds, hydrophobic interaction and π-π interplay. With M-FH-POP as adsorbent, a magnetic solid phase extraction method was established for extracting trace phenolic EDCs (bisphenol A, 4- tert -butylphenol, bisphenol F and bisphenol B) in water and fish before ultra-high performance liquid chromatography tandem mass spectrometry analysis. The method displayed low detection limit (S/N = 3) of 0.05–0.15 ng mL −1 for water and 0.08–0.3 ng g −1 for fish. The spiked recoveries were 88.3 %–109.8 % with the relative standard deviations of 2.4 %–6.4 %. The method offers a new strategy for sensitive determination of phenolic EDCs in water and fish samples.
研究领域:
环境科学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Juan Wang,Xiangsheng Hong,Wang Liu,Le Zhang,Saihong Yan,Zhitong Li,Jinmiao Zha
As substitutes for bisphenol A (BPA), bisphenol analogs (BPs) have raised concerns due to their frequent environmental detection and unclear safety. Here, the cytotoxicity, endocrine disruption, neurotoxicity, aryl hydrocarbon receptor (AhR) activity, and genotoxicity of nine BPs and BPA were evaluated in three types of cell lines. Over half of the tested BPs exhibited greater cytotoxicity than BPA, with IC50 values showing a linear correlation with Log Kow (R²=0.69). All tested BPs exhibited at least one endocrine-disrupting effect, notably estrogenic, which was observable even at 0.01–0.1 μM. Importantly, BPAF and BPAP exposure had widespread endocrine-suppressing effects. Moreover, all BPs (except BPP) and BPA increased SH-SY5Y cells apoptosis at 1–10 μM. Only BPF and BPP significantly increased 7-ethoxyresorufin-O-deethylase levels, highlighting their notable effects on AhR activity. BPAF significantly induced DNA damage at 1.25 μM, whereas BPA, BPF, and BPP induced damage at 20, 25, and 25 μM, respectively. Finally, ToxPi, a weighted scoring system, was used to rank the comprehensive toxicity of BPs, with 7 of 9 BPs showing higher scores than BPA. Collectively, BPs generally exhibited stronger comprehensive toxicity compared with BPA, emphasizing the urgent need for further research to confirm their potential health implications.
研究领域:
毒理学
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环境科学
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生物医学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Li Wang,Lin Liu,Runan Chen,Ya Jiao,Kaixin Zhao,Yongli Liu,Guifen Zhu
Highly selective removal of residual cephalosporin antibiotics from complex systems is crucial for human health and ecological environment protection. Herein, a newly molecularly imprinted polymer adsorbent (CPDs-NH 2 @MIP) with enhanced selectivity for ceftiofur sodium (CTFS) was developed by using the special carbonized polymer dots (CPDs-NH 2 ) as functional monomer . The CPDs-NH 2 has a nano-spherical structure and functionalized groups (C C, −NH 2 ) via the incomplete carbonization polymerization of citric acid , acrylamide and ethylenediamine , which can accurately interact with CTFS by overcoming steric hindrance , resulting in more precisely imprinted sites and reducing non-imprinted regions in MIP. The presented CPDs-NH 2 @MIP exhibited excellent adsorption capacity for CTFS (68.62 mg g −1 ), achieving equilibrium within 10 min, and highly selectivity in mixed solution containing five coexisting substances, with an imprinted factor (5.61). Compared with commercial adsorbents and MIPs prepared with traditional chain functional monomers, the CPDs-NH 2 @MIP showed significant advantage in selective recognition and separation of target. Analysis of microstructure and mechanism proved that usage of the spherical functional monomer generated precise imprinting sites and dense structure in CPDs-NH 2 @MIP, which effectively enhanced the selectivity in complex system combined with hydrogen bonding interaction. The idea of designing and using spherical functional monomer will promote the practicality of molecularly imprinted polymer adsorbents.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Zhenjun Xiao,Junlong Zhang,Yijie Lin,Kaixuan Zheng,Zili Lin,Mengyang Niu,Wanlin Lin,Yuchun Hong,Zhongyang Li,Ping Chen,Qingbin Song,Wenying Lv,Guoguang Liu
Developing new technologies to address emerging contaminants are urgently needed. To this end, a novel advanced oxidation system (US/CaSO 3 /PDS) utilizing ultrasound to activate persulfate (PDS) and calcium sulfite (CaSO 3 ) for the effective degradation of emerging contaminants was constructed in this study. The pseudo first-order kinetics constant of Phoxim (PHO) can reach 4.3 × 10 −2 min −1 in US/CaSO 3 /PDS system, revealing the prospects for the treatment of pollutants in water. Electron paramagnetic resonance spectra demonstrated that persulfate anion radicals, hydroperoxyl radicals, hydroxyl radicals, and singlet oxygen were produced in the system. Further, quenching experiments and reactive species probes were used to verify the chemical reaction mechanism of this novel system, and the experimental results highlight the key role of 1 O 2 in the degradation of pollutants thanks to the participation of oxygen. In addition, 11 main products in the PHO degradation process were identified by liquid chromatography-high-resolution precise mass spectrometry, and the possible degradation path of PHO in US/CaSO 3 /PDS system was proposed. US/CaSO 3 /PDS system had high pollutant removal efficiency against a variety of emerging pollutants and was applicable in real water matrix. This study provides a new scheme for the development of advanced oxidation technology based on PDS activation for the removal of refractory emerging pollutants in water.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）