ANALYTICAL LETTERS
·
2025
·
IF 1.8
Xiu Fan,Xiaoyan Li,Yihua Wang,Dawei Xu,Liping Zhao,Ruilan Yuan,Chonghua Hao,Chunyu Wang
Staphylococcus aureus (S. aureus) is globally recognized as a foodborne pathogen that causes food poisoning by secreting enterotoxins. In this study, a novel dual-recognition strategy for detecting S. aureus was developed, employing vancomycin-functionalized magnetic beads (Van-MBs) for target capture and canine immunoglobulin G (IgG) to selectively and sensitively bind to staphylococcal protein A (SPA) on the bacterial surface. Dual-recognition strategies based on vancomycin and porcine IgG have previously been developed for the detection of S. aureus. However, porcine IgG can also bind to protein G, which poses the risk of generating false-positive signals from protein G-producing Streptococci. In contrast, canine IgG demonstrates a higher affinity for protein A than for protein G. When S. aureus is present in the sample matrix, the Van-MBs and biotin-labeled canine IgG successfully form a sandwich complex. Then, streptavidin-conjugated horseradish peroxidase (HRP) is added, which binds to the sandwich structure. HRP then catalyzes the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), resulting in the formation of a colorimetric signal that was proportional to the concentration of S. aureus within the concentration range of 6.3 × 103–4.0 × 105 CFU/mL with a detection limit of 1.9 × 103 CFU/mL. By substituting porcine IgG with canine IgG, this method eliminates cross-reactivity with protein G-expressing Streptococci, achieving specificity over non-target species. This approach highlights its potential for application in diverse sample matrices, including clinical and biological specimens.
研究领域:
微生物学
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免疫学
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食品卫生
·
诊断检测
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Shuhuai Li,Xionghui Ma,Chaohai Pang,Haibo Li,Chunhua Liu,Zhi Xu,Jinhui Luo,Yan Yang
The use of dual recognition and multiple detection modes is an attractive strategy for realising sensors with improved selectivity and accuracy. Herein, a molecularly imprinted polymer (MIP)-based sensor is developed for amoxicillin detection based on two detection modes (fluorescence and electrochemiluminescence) and dual recognition. First, graphene oxide loaded with CdTe quantum dots/gold nanoparticles (GO/CdTe/Au NPs) is coated onto an indium tin oxide (ITO) electrode. Then, 4-mercapto-calix[6]arene is bonded to GO/CdTe/Au NPs as the first recognition element, which then form a host–guest complex with the target molecule amoxicillin. Subsequently, as the second recognition element, an MIP is prepared on the ITO electrode. After amoxicillin is removed from the MIP, specific identification sites for amoxicillin are obtained. Furthermore, the GO/CdTe/Au NPs can generate fluorescence and electrochemiluminescence signals that are effectively quenched by amoxicillin. Therefore, on/off switching of these signals can be achieved through the elution or adsorption of amoxicillin. The dual detection modes are complementary and provide mutual authentication, which can improve the detection accuracy and application scope. Moreover, the dual recognition sites for amoxicillin, improve detection selectivity. The fluorescence and electrochemiluminescence modes have detection ranges of 5–1000 × 10 −11 mol L −1 and 5–1500 × 10 −11 mol L −1 , respectively, with detection limits of 9.2 × 10 −12 mol L −1 and 8.3 × 10 −12 mol L −1 , respectively.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
DOI
高影响因子
Journal of the American Chemical Society
·
2025
·
IF 15.6
Yeqing Xu,Abuduheiremu Awati,Shan Zhou,Runhao Zhang,Xin Zhang,Hui Zeng,Yaxin Guo,Yuanbo Song,Chengmin Hu,Lei Xie,Qirui Liang,Kang Liang,Lei Jiang,Dongyuan Zhao,Biao Kong
Pesticides are vital for crop and seafood production but leave persistent residues that pose risks to ecosystems and human health through bioaccumulation. The detection of pesticide residues requires highly sensitive and selective technologies. Herein, a nanochannel sensor capable of dual recognition of ionic charge and molecular conformation based on molecular imprinting technology (MIT) is presented, offering a significant improvement in selectivity and sensitivity over traditional nanopore sensors. The MIT-based nanochannels with imprinting sites tailored to pesticide molecules go beyond recognizing the molecular size and surface functional groups, enabling the detection of molecular configurations. In this research, the approach enables the detection of 10 pesticide molecules with detection limits (LODs) ranging from 12.9 to 26.9 pM, achieving two orders of magnitude lower than fluorescence-based methods. Density functional theory (DFT) and molecular dynamics (MD) simulations revealed hydrogen bonding as the dominant interaction in the imprinting process. This versatile nanochannel construction method, proposed for the first time, provides dual recognition capabilities and is expected to advance nanochannel sensing while promoting sustainable environmental development.
研究领域:
环境科学
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生物传感技术
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
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IF 5.31
Shucheng Liu,Jianming Pan,Hengjia Zhu,Guoqing Pan,Fengxian Qiu,Minjia Meng,Juntong Yao,Dong Yuan
In this work, graphene oxide (GO) based molecularly imprinted polymers with double recognition abilities (DR-MIPs) were prepared and considered as adsorbent for the specific recognition and capture of luteolin (LTL). To exhibit the tightest binding hosts for LTL, the double recognition abilities were achieved by adopting 4-vinylphenyl boronic acid (BA) and methacrylic acid (MAA) to be the covalent and non-covalent imprinted monomers, respectively. Then, their functional groups and shape of imprinted sites endowed DR-MIPs with a specific affinity for cis-diol-containing structure, hydroxyl and carbonyl groups of LTL. The results of batch mode experiments indicated kinetic equilibrium time and binding capacity of DR-MIPs were 30 min and 56.27 mg g −1 at 298 K, respectively. The Langmuir isotherm and pseudo-second-order kinetic models were the main adsorption mechanisms for DR-MIPs, and the fast adsorption and large binding amount were resulted from the two-dimensional (2D) structure of GO and enough imprinted sites with double recognition abilities. DR-MIPs also showed excellent recognition ability, and the estimated relative selectivity coefficients ( k ′) for structural analog quercetin (QRT), hydroquinone (HDQ) and p-nitrophenol (P-NP) were 13.73, 18.62 and 19.95, respectively. In addition, DR-MIPs possessed outstanding reusability and enhanced purification property for 85% raw LTL. The purified LTL products achieved approximately 93.47%, and they exhibited the obvious antibacterial performance.
研究领域:
材料科学
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分析化学
✓ 已验证
DOI
高影响因子
Journal of Analytical Science and Technology
·
2025
·Vol. 16
·期 1
·1-10
·
IF 2.8
Zhang Yun,Huo Chunhui,Yang Xiaohong,Cui Yanjie,Hou Yiqing,Guo Qinghe,Wang Qiongzi,Wang Mingyong,Zhang Yi
Staphylococcus aureus ( S. aureus ) is one of the major food-borne pathogenic bacteria that causes epidemic and food poisoning. In this study, a colorimetric sensing platform was developed for the sensitive and selective detection of S. aureus based on the synergy between 3-aminophenylboronic acid-functionalized magnetic beads (APBA-MBs) and horseradish peroxidase labeled anti-protein A IgY (HRP-IgY). Target S. aureus bacteria can be captured on the APBA-MBs and then labelled by HRP-IgY through formation of a sandwich structure. Detection of the bacteria then involves oxidation of the colorimetric HRP substrate, thereby converting S. aureus concentrations into a detectable signal. The optimized method can be performed within 120 min, with a detection limit of 9.4 × 10 2 CFU/mL . The proposed strategy was successfully applied to detection of S. aureus in spring water and apple juice samples. The dual-recognition platform showed selectivity towards S. aureus , and other common foodborne pathogens yielded negative results. The incorporation of IgY into the proposed strategy can address the lack of selectivity observed in previously reported APBA-based methods. Our strategy has potential as a versatile platform for the sensitive detection of various bacteria using corresponding IgY.
研究领域:
食品安全
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生物传感
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病原体检测
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Shuhuai Li,Jianping Li,Xionghui Ma,Chaohai Pang,Guihao Yin,Jinhui Luo
A dual selection/recognition effect is described for cobalt (II) ions (Co 2+ ). It is based on bovine serum albumin-metal-Co 2+ coordination (BSA-Co 2+ ) and the use of a molecularly imprinted polymer (MIP). An electrochemiluminescence (ECL) switch sensor was designed for detecting nanomolar levels of Co 2+ . The BSA-Co 2+ coordination complex was chosen as a template to prepare a MIP modified switch sensor. The coordination reaction between BSA and Co 2+ provides the first step in recognition, and MIP provides the second step for Co 2+ . This leads to a strong improvement in selectivity of the sensor. A multi-walled carbon nanotube/Cu nanoparticles/carbon quantum dots nanocomposite (MWCNT/Cu/C-dots) acts as an ECL device, and the BSA-Co 2+ complex quenches the ECL signal. Therefore, the elution and resorption of BSA-Co 2+ can be used as a switch to control ECL. Additionally, a method was established to detect Co 2+ , with the detection limit as low as 3.07 × 10 −10 mol/L. The method was applied to the analysis of spiked environmental water, soil samples, and agricultural products. The recovery rates of the method were in the range of 87.5–111.3%.
研究领域:
分析化学
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环境监测
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食品安全
·
分子识别
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生物传感
·
纳米材料
·
电化学分析
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Ailing Su,Yuan Liu,Weihan Sun,Chongyang Liang,Weiqing Xu,Alison Rodger,James Piper,Yuling Wang,Shuping Xu
Specifically identifying one-base mismatched gene sequences is of significance for early diagnosis, prognosis, treatment, and evaluation. However, traditional polymerase chain reaction and next-generation sequencing methods require relatively long, carefully implemented analytical procedures. Here, we report a nanoconjugate that can synchronously discriminate two types of KRAS gene mutation due to the dual-recognition mechanism of the CRISPR/dCas system with the surface-enhanced Raman scattering (SERS) response output. This method applied two sets of CRISPR/dCas9 units to collect all KRAS genes via a magnetic force and the specific mutant sequence labeled through SERS nanotags, forming a dual-clamping architecture. This sensing conjugate was sufficient to quantify the amount of the two different mutations simultaneously with a low cross-reactivity. Consequently, we deem that this sensing assay satisfies the great characteristics of a genetic analytical tool with high specificity, accuracy, and simplicity, highlighting its potential as an alternative for screening multigene events and relevant genetic biomarkers.
研究领域:
分子生物学
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纳米技术
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基因工程
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生物医学工程
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癌症研究
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Juan Cai,Huawei Shen,Yonghong Wang,Yang Peng,Sitian Tang,Yalin Zhu,Qian Liu,Baiying Li,Guoming Xie,Wenli Feng
Accurate detection of circulating tumor cells (CTCs) has a pivotal role in the metastasis monitoring and prognosis of tumor. In this work, an ultrasensitive electrochemical cytosensor was developed based on excellent electrocatalytic materials and a dual recognition strategy. Herein, novel branched PtAuRh trimetallic nanospheres (b-PtAuRh TNS) were synthesized for the first time by a facile one-pot method, which had a huge specific surface area and outstanding catalytic activity. B-PtAuRh TNS linked with aptamers targeting mucin1 (MUC1) were served as signal tags to amplify the signal. As electrode modified material, the nanocomposites of Cabot carbon black (BP2000) and AuNPs were used to improve the electron transfer efficiency of electrode. In addition to using b-PtAuRh TNS labeled anti-MUC1 aptamers as signal probes, anti-EpCAM antibodies were worked as capture probes to achieve dual recognition of target cells. In other words, only cells expressing both MUC1 and EpCAM could produce electrochemical signal. The constructed cytosensor presented a wide linear range (5 - 1 × 10 6 cells mL −1 ) and a low detection limit (1 cell mL −1 ). It was worth noting that the proposed cytosensor could detect CTCs in clinical blood samples. To sum up, the developed cytosensor might become a promising detection platform for cancer diagnosis and tumor metastasis.
研究领域:
生物医学工程
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纳米技术
·
癌症研究
·
电化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2022
·
IF 6.212
Xiaobin Wang,Wei Guo,Xiaoling Wang,Qing Hua,Feiyan Tang,Xin Li,Feng Luan,Zhiyang Zhang,Chunyuan Tian,Xuming Zhuang,Lijun Zhao
In the present study, a dual recognition strategy for ultrasensitive detection of Hg 2+ was successfully developed for the first time based on aptamer functionalized sulfur quantum dots (Apt-SQDs). The developed Apt-SQDs not only retained the good fluorescence properties of quantum dots but also overcame the problem of poor selectivity of SQDs for heavy metal ions. This system used the dual recognition strategy, including the combination of S x 2− and Hg 2+ and T-Hg 2+ -T structures to excellently identify and capture Hg 2+ , and an ultrahigh sensitivity fluorescent aptasensor was fabricated. The fluorescent aptasensor had a good response to Hg 2+ at concentrations ranging of 10 −15 to 10 −7 M with an ultralow limit of detection of 0.3 fM, and the response to other metal ions was far less than that to Hg 2+ . It was successfully applied to detect Hg 2+ in nearby environmental water samples (tap water, lake water and river water) with a good recovery rate. Moreover, portable test papers that would be useful for Hg 2+ monitoring in environmental water were designed. The dual recognition strategy not only achieves ultrasensitive fluorescent detection of Hg 2+ but also provides a new insight into the further expansion of the application of SQDs.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ding Yang,Yuanyuan Hui,Yingying Liu,Weizhe Wang,Chao He,Aiqing Zhao,Lusha Wei,Bini Wang
Aflatoxin M 1 (AFM 1 ) is a highly carcinogenic and toxic compound that is commonly found in dairy foods such as milk. We developed an electrochemical MIP (molecularly imprinted polymer) and aptamer (Apt) based aptasensor (Apt-Au@PEIM/AFM 1 /MIP–Apt/AuNPs)/GCE) with dual-recognition signal amplification to detect AFM 1 in milk. The MIP–Apt/AuNP/GCE sensor was prepared by synthesizing an MIP film through the electropolymerization of resorcinol on gold nanoparticles-modified electrodes. The dropwise addition of our cApt-Au@PEIM signal amplification probe could enhance the current signal response and improve the sensitivity of the sensor to detect AFM 1 . The AFM 1 present in milk could prevent the probe from binding to the sensor, thereby drastically reducing the current signal. Under optimal experimental conditions, the sensor exhibits a linear range of 0.01–200 nM and a limit of detection of 0.07 nM (S/N = 3). The developed dual-recognition aptasensor would provide a novel and rapid method for specific detection of AFM 1 in milk.
研究领域:
食品安全
·
分析化学
·
电化学
·
生物传感器
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Mingzhen Zhu,Shuyun Meng,Shuda Liu,Yuqing Li,Na Dong,Zuo Chen,Wenjia Li,Dong Liu,Tianyan You
Sandwich-type biosensor allows better accuracy for detection, yet dual recognition with two free bioreceptors is prone to interference and requires extended assay time. This study reported a ratiometric electrochemical aptasensor with a confined sandwich structure via the reunion of two split aptamers for 17β-estradiol (E2) detection. A sandwich-type bioreceptor was synthesized to confine dual recognition by connecting two split aptamers with thymine (T) bases, and the number of T bases showed significant influence on its performance. For biosensing, the ferrocene (Fc)-labelled sandwich-type bioreceptor caught E2 to produce an enlarged current signal of Fc (I Fc ); methylene blue (MB)-loaded zeolitic imidazolate framework-8 nanocomposite, absorbed by porous laser-induced graphene to act as substrate electrode, output a stable current signal of MB (I MB ) for reference. Using I Fc /I MB as a yardstick, the fabricated ratiometric electrochemical aptasensor offered a linear range of 10 pg mL −1 -500 ng mL −1 with a detection limit of 7.60 pg mL −1 for E2 detection as well as high selectivity, stability, and reproducibility. The sandwich-type bioreceptor by split aptamer reunion provided a high-performance recognition element for biosensors, and this work should pave a new way to obtain highly efficient bioreceptors.
研究领域:
生物传感器
·
分析化学
·
生物化学
·
纳米技术
·
环境监测
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Ya Cao,Yuhao Dai,Hong Chen,Yingying Tang,Xu Chen,Ying Wang,Jing Zhao,Xiaoli Zhu
In this work, DNA-templated silver nanoclusters (DNA-AgNCs) with unique fluorescent and electrochemical properties are prepared as dual signal probes for both qualitative imaging and quantitative detection of cancer cells in an integrated system . ITO electrode that has good light transmittance and electric conductivity is employed as a substrate for dual analysis of cancer cells. ITO electrode is firstly modified by AS1141 aptamer , which could selectively bind to nucleolin overexpressed on the surface of a model breast cancer cell, MCF-7 cell line. The composite of mucin 1 antibody (anti-MUC1) and DNA-AgNCs then binds to MUC1 on the surface of captured MCF-7 cell, forming a sandwich-like structure. Therefore, our method allows noninvasive fluorescence imaging and amplified electrochemical detection using a single labeling platform, providing a biocompatible and highly specific method for adequate analysis of cancer cells. Experimental results demonstrate that strong red fluorescence of DNA-AgNCs clearly displays the loading of cancer cells on ITO electrode after dual recognition, and amplified electrochemical signals of DNA-AgNCs enable improved sensitivity toward quantitative analysis with a detection limit of 3 cells.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）