CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xiaomin Tan,Yanjuan Huang,Yong Luo,Zijun Lin,Xianmin Shi,Zishan Zeng,Jie Chen,Chunshun Zhao
Colon cancer remains the second leading cause of cancer-related deaths, necessitating advanced early diagnostic tools to improve survival outcomes. Leveraging the tumor-specific overexpression of hydrogen sulfide (H 2 S) in colon cancer, various H 2 S-responsive probes have been developed. However, conventional H 2 S fluorescent probes suffer from interference by commonly expressed nitroreductase (NTR) in normal colonic tissues due to shared activation mechanisms. Here, we proposed an innovative size-exclusion strategy utilizing metal-organic cages (MOCs) to engineer a highly-selective H 2 S probe (MOC-HSN1). MOCs was synthesized by coordinating BPAB with palladium ions, and a classic H 2 S-responsive probe HSN1 was concurrently loaded to form MOC-HSN1. With a molecular weight of 10–20 kDa, the MOCs function as a molecular size sieve, which selectively excludes NTR (200–220 kDa) from the outside while permitting H 2 S (34 Da) molecules to enter the inside and activate HSN1, thereby improving the selectivity of H 2 S imaging to achieve precise colon cancer diagnosis. Results showed that MOC-HSN1 had good stability, biocompatibility, and it enabled precise qualitative and quantitative H 2 S detection in vitro, in cells, and in vivo. This size-discrimination strategy effectively circumvents NTR interference, positioning MOC-HSN1 as a translatable platform for integration with fluorescence endoscopy, with potential to augment early colorectal cancer localization and diagnosis in clinical settings.
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Advanced Healthcare Materials
·
2023
·Vol. 12
·期 15
·2203078
·
IF 10
Yanhao Li,Chengfang Hu,Bo Hu,Jian Tian,Gang Zhao,Chuandong Cai,Yuange Li,Zhenyu Sun,Shuo Wang,Sa Pang,Rong Bao,Zaijing Tao,Huajiang Chen,Jinglei Wu,Shen Liu
The prevention and treatment of post-traumatic peritendinous adhesion (PA) have always been a great difficulty for orthopedic surgeons. Current treatments include resecting surgery, non-steroidal anti-inflammatory drugs (NSAIDs) usage and implantable membranes, often target single disease pathogenic processes, resulting in unfavorable therapeutic outcomes. Here a polylactic acid (PLA)-dicumarol conjugates-electrospun nanofiber membrane (ENM) (PCD) is generated, which can achieve spatial accuracy and temporal sustainability in drug release. It is further demonstrated that PCD possesses a significantly higher and more sustainable drug release profile than traditional drug-loading ENM. By providing a physical barrier and continuous releasing of dicumarol, PCD implantation significantly reduces tissue adhesion by 25%, decreases fibroblasts activity and inhibits key fibrogenic cytokine transforming growth factor beta (TGF β ) production by 30%, and improves the biomechanical tendon property by 14.69%. Mechanistically, PCD potently inhibits the connexin43 (Cx43) and thereby tunes down the fibroblastic TGF β /Smad3 signaling pathway. Thus, this approach leverages the anti-adhesion effect of dicumarol and drug release properties of grafted copolymer ENM by esters to provide a promising therapeutic strategy for patients who suffer from PA.
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ANALYTICAL CHEMISTRY
·
2022
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IF 8.008
Yi-Xuan Fu,Wu-Yingzheng Guo,Nan Wang,Yi-Jie Dai,Zi-Ye Zhang,Xin-Lin Sun,Wen-Chao Yang,Guang-Fu Yang
Plant diseases caused by bacteria have become one of the serious problems that threaten human food security, which led to the remarkable reduction of agricultural yields and economic loss. Nitroreductase (NTR), as an important biomarker, is highly expressed in bacteria, and the level of NTR is closely related to the progression of pathogen infection. Therefore, the design of small-molecule fluorescent sensors targeting NTR is of great significance for the detection and diagnosis of plant pathogenic bacteria. In this study, a new fluorescent sensor targeting NTR was discovered and then successfully applied to the imaging of zebrafish and pathogenic bacteria. Most importantly, the developed sensor achieved the real-time diagnosis of Brassica napus L. infected with bacteria, which provides a promising tool for examining the temporal and spatial infection of plant pathogens in precision agriculture.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xianghan Zhang,Zhiqing Gao,Yuqiong Xia,Qunyan Dong,Yutian Cao,Qian Jia,Fang Sun,Zheng Li,Chu Tang,Jie Yu
Nitroreductase (NTR) detection in tumor is critical because NTR level is correlated with hypoxia degree and cancer prognosis. With the feature of high sensitivity and selectivity, fluorescence organic probes for NTR detection exhibited a promising future for tumor hypoxia detection. However, the discovery and design of such probes have been impeded due to the lack of the understanding of spatial match and mismatch of these probes with NTR. Here, we have developed two new nitrophenyl-functionalized trimethincyanine (Cy3) probes with para- or meta- positions of nitro-group in phenyl ring. Para-nitrophenyl substituted Cy3 (pNP-Cy3) exhibited a remarkable response to NTR (20-fold fluorescence enhancement) with good selectivity and sensitivity. Experimental and theoretical analysis verified that the substituent position of nitro group on phenyl ring of dyes altered the spatial arrangement of nitro-substituent group, thereby modulated the spatial match and mismatch between Cy3 dyes and binding domain of NTR, and consequently led to a different fluorescent turn-on response. In tumor-bearing mice model, hypoxia status of A549 xenografted tumor of mice was successfully delineated by using pNP-Cy3. These results may provide a clue for designing new cyanine-derived NTR probe to monitor NTR-overexpressed hypoxia cancer cells.
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DRUG DELIVERY
·
2021
·
IF 6.419
Bingfeng Liang,Dangxia Zhou
Although podophyllotoxin (POD) demonstrates high efficiency to inhibit various cancers, its clinic application is limited to poor bioavailability. Nanoparticles derived from homodimeric prodrugs with high drug loading potential are emerging as promising nanomedicines. However, complete intracellular drug release remains a major hindrance to the use of homodimeric prodrugs-based nanomedicine. We sought to develop a reactive oxygen species (ROS) responsive POD dimeric prodrug by incorporating vitamin K3 (VK3) and Pluronic F127 to synthesize a spheroid nanoparticle (PTV-NPs). PTV-NPs with high POD content could release drugs under the ROS enrichment microenvironment in cancer cells. The released VK3 could produce abundant ROS selectively in tumor cells catalyzed by the overexpressed NAD(P)H: quinone oxidoreductase-1 (NQO1) enzyme. In turn, the resultant high ROS concentration promoted the conversion of POD dimeric prodrug to POD monomer, thereby achieving the selective killing of cancer cells with weak system toxicity. In vitro and in vivo studies consistently confirmed that PTV-NPs exhibit high drug loading potential and upstanding bioavailability. They are also effectively internalized by tumor cells, induce abundant intracellular ROS generation, and have high tumor-specific cytotoxicity. This ROS-responsive dimeric prodrug nanoplatform characterized by selective self-amplification drug release may hold promise in the field of antitumor drug delivery.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yamei Ma,Zhen Zhang,Shengchao Gao,Yue Zhan,Zihao Wei,Ying Liu
In this study, the performance of microbial fuel cells (MFCs) in azo-dye decolorization coupled with electricity generation was evaluated via the model exoelectrogens G. sulfurreducens PCA. The results showed that G. sulfurreducens PCA was capable of decolorizing Congo red (CR) while producing electricity in MFCs. The extracellular reduction of CR by G. sulfurreducens PCA requires the addition of carbon substrate (20 mM NaAc) to generate electrons and then transfer them to the exterior of the cell through respiratory chain proteins. The removal efficiency of 300 mg L −1 CR in the anodic chamber MFCs (98.5%) was found to be 2.30 times higher than that of traditional anaerobic decolorization (42.9%), whereas the maximum output voltage of MFC (0.184 ± 0.011 V) was reduced by 65.2% when compared to that without the addition of CR (0.529 ± 0.014 V). By reducing the initial CR concentration from 300 mg L −1 to 200 mg L −1 and 100 mg L −1 , the maximum output voltage of MFCs systems was increased to 0.260 ± 0.042 V and 0.392 ± 0.034 V, respectively, which were 41.3% and 113.0% higher than the 300 mg L −1 CR. Thus, our study stressed that the efficient azo dye decolorization in conjunction with bioelectricity generation could be realized using the MFCs system with model exoelectrogens G. sulfurreducens PCA as the biocatalyst.
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BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Xing Yan,Ming-Jun Zhu
Microbial fuel cell (MFC) with lignocellulose as an electron donor is considered a sustainable biorefinery. However, low lignocellulose degradation and energy output restrict the scale of application. Herein, the extracellular electron transfer (EET) capacity of Acetivibrio thermocellus DSM 1313 with lignocellulose as substrate was shown to be mediated by the self-produced flavin, and its intracellular electron transfer went through the whole respiratory chain. Thermophilic MFC with resazurin exhibited an increase in the open circuit voltage by 37.78%, and a 2.60 folds increase in power density of 77.85 mW/m 2 , respectively. Differential pulse voltammetry and electrochemical impedance spectroscopy analysis indicated that resazurin decreased the solution and anode charge transfer resistance, and enhanced the extracellular electrochemical activity. Furthermore, resazurin resulted in a lower redox potential, allowing preferential electron transfer to resazurin rather than flavin. This research establishes a resazurin-mediated thermophilic MFC with lignocellulose as substrate, which provides novel idea on the biomass refinery.
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Molecular Medicine Reports
·
2024
·Vol. 29
·期 6
·1-11
·
IF 3.4
Wenjie Ge,Xian Zhang,Qing Wang,Jianjie Mao,Pengfei Jia,Jianping Cai
Knee osteoarthritis (KOA) is a major cause of disability in elderly individuals. Dicoumarol is a coumarin‑like compound derived from sweet clover [Melilotus officinalis (L.) Pall]. It has been suggested that dicoumarol exhibits various types of pharmacological activities, including anticoagulant, antitumor and antibacterial effects. Due to its various biological activities, dicoumarol has a potential protective effect against OA. Therefore, the present study aimed to assess the effects of dicoumarol on knee osteoarthritis. In the present study, dicoumarol was found to protect rat synoviocytes from lipopolysaccharide (LPS)‑induced cell apoptosis. Western blot analysis showed that dicoumarol significantly reduced the protein expression levels of fibrosis‑related markers and inflammatory cytokines (Tgfb, Timp, Col1a, Il1b and Il18). The inhibitory rates of these proteins were all >50% (P<0.01) compared with those in the LPS and ATP‑induced group. Consistently, the mRNA expression levels of these markers and cytokines were decreased to normal levels by dicoumarol after the treatment of rat synovial fibroblasts with LPS and ATP. Mechanistic studies demonstrated that dicoumarol did not affect NF‑κB signaling, but it did directly interact with NOD‑like receptor protein 3 (NLRP3) to promote its protein degradation, which could be reversed by MG132, but not NH4Cl. The protein half‑life of NLRP3 was accelerated from 26.1 to 4.3 h by dicoumarol. Subsequently, dicoumarol could alleviate KOA in vivo; knee joint diameter was decreased from 11.03 to 9.93 mm. Furthermore, the inflammation and fibrosis of the knee joints were inhibited in rats. In conclusion, the present findings demonstrated that dicoumarol could impede the progression of KOA by inhibiting NLRP3 activation, providing a potential treatment strategy for KOA.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Linna Chang,Yan Qin,Zhou Shen,Di Wu,Xiaoping Yin,Leyong Zeng
Reactive oxygen species (ROS) are critical in tumor therapy, but the antioxidant tumor microenvironment severely restricts the ROS-modulated antitumor efficacy. In this study, a photothermal-promoted cascade nanozyme (MnO 2 @Lap/PAH/L-Arg@Au) was constructed by modifying β-lapachone (β-Lap), poly(allylamine hydrochloride) (PAH), and L-arginine (L-Arg) and depositing gold nanoparticles (AuNPs) on hollow manganese dioxide (MnO 2 ), enhancing ROS-modulated catalytic/gas combination therapy. The pH- and glutathione-responsive hollow MnO 2 degradation released β-Lap to elevate the generation of hydrogen peroxide (H 2 O 2 ), which substantially activated and AuNPs-mediated hydroxyl radical (·OH) generation. Moreover, H 2 O 2 /·OH induces L-Arg to produce highly toxic nitric oxide (NO), and the Au nanoshell with high photothermal conversion efficiency (38.6 %) further enhanced ROS/NO generation. By reducing adenosine triphosphate production and inducing mitochondrial dysfunction, the combination of photothermal/catalytic/gas therapy greatly triggered cell apoptosis and inhibited tumor growth. Thus, the developed MnO 2 @Lap/PAH/L-Arg@Au nanozyme established a dynamic cascade pathway of “ROS-driven NO release”, and achieved photothermal-promoted catalytic/gas therapy.
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ACS Nano
·
2025
·
IF 16
Yanjing Yun,Weitao Yang,Yan Xu,Kexin Bian,Dinghua Liu,Hui Wang,Chang Liu,Shuang Wu,Bingbo Zhang
Hypoxia, a hallmark of the tumor microenvironment, promotes cancer aggressiveness and treatment resistance by activating HIF-mediated signaling pathways while compromising the efficacy of radiotherapy, chemotherapy, and targeted therapy. Current hypoxia imaging techniques suffer from invasiveness, limited specificity, or insufficient spatial resolution. To address these challenges, we developed hypoxia-responsive magnetic resonance imaging (MRI) nanoprobes (FAP NPs) composed of 4,4′-azobisbenzoic acid (AZB) and Fe3+. These nanoprobes exploit the overexpression of azoreductase in hypoxic tumors, triggering the selective reduction of the azo bond and the subsequent release of paramagnetic Fe3+ ions. This structural transformation amplifies proton relaxivity by 12-fold (r1: 0.1–1.2 mM–1 s–1), significantly enhancing T1-weighted MRI contrast for precise hypoxia mapping. In vivo studies showed that FAP NPs detect hypoxia with high sensitivity in pancreatic, colorectal, and breast tumors of varying sizes (5–14 mm) and allow quantitative monitoring of changes in hypoxia levels following pancreatic tumor chemotherapy. Furthermore, FAP NPs stratified tumors into normoxic and hypoxic subgroups, accurately predicting radiotherapy efficacy. By integrating enzyme-responsive activation with Fe3+-mediated signal amplification, FAP NPs provide a noninvasive platform for real-time hypoxia assessment, guiding personalized therapeutic strategies and optimizing outcomes in solid tumors.
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