JOURNAL OF MEMBRANE SCIENCE
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2022
·
IF 10.53
Hao Zhang,Luke Liu,Manuel Pinelo,Yuhong Huang,Weiqing Zhou,Yinhua Wan,Jianquan Luo
Fabricating high-performance enzyme reactors is requested for achieving efficient and stable bioconversions, but remains challenging, because few of them can possess high enzyme loading, sufficient mixing, and efficient mass transfer at the same time. Herein, we propose to develop a novel enzymatic packed bed membrane reactor (EPBMR) by integrating the advantages of both packed bed reactor (PBR) and enzymatic membrane reactor (EMR). A prototype study is conducted with the simplified enzyme-loaded microsphere-ultrafiltration EMR model (Mic-UF EMR). Invertase and dextranase are used in this work to produce glucose and oligodextran by hydrolysis of sucrose and dextran , respectively. Specifically, the use of microspheres can enlarge the contact area between enzymes and substrates and mitigate membrane fouling induced by free enzymes. Thus, Free&Mic-UF EMR (with both free and immobilized enzymes) exhibits a higher sucrose conversion rate (84%) than the EMR with free invertase (34%) and a negligible decline in sucrose conversion for 36 h continuous operation. Membrane fouling is ameliorated by alkaline cleaning and implementation of covalent bonding strategy. In addition, commercial resins with larger sizes are employed to replace konjac glucomannan microspheres (KGM) which reduce the pressure drop of EMR. Finally, by selecting the UF membrane with proper molecular weight cut-off (MWCO), the dextranase-based Mic-UF EMR system successfully produces oligodextran with desired molecular weight (Mw) and narrow Mw distribution. The outcome of this work not only offers a novel enzyme reactor construction strategy but also provides guidance for regulating the performance of EMR.
研究领域:
生物技术
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化学工程
·
生化工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Flow Chemistry
·
2021
·Vol. 11
·期 2
·171-179
·
IF 2.786
Lan Zhou,Lu Yangcheng
The matching of the micro-dispersion method and the reaction kinetics is of the utmost importance for the fast and strongly exothermic nitration reaction. Taken as an example to discuss this issue, a synthesis of 3,4-dichloronitrobenzene via the nitration of o -dichlorobenzene with mixed acid of nitric acid and sulfuric acid was conducted in continuous-flow reactors in this work. Through combining the adiabatic reaction environment, the use of a micropacked-bed reactor, and introducing partial product circulation, it successfully achieved the matching of the micro-dispersion state and the reaction kinetic characteristics. The good two-phase dispersion state can be maintained throughout the micropacked-bed reactor and the reaction can be completed within 5 s with above 89% selectivity under adiabatic condition. Furthermore, the reaction activation energy was conveniently obtained by processing the temperature distribution data, providing a fundamental to realize a reliable design of the nitration reaction process with high efficiency and inherent safety. Graphical abstract
研究领域:
化学工程
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反应动力学
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产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 86
·70139-70146
·
IF 3.84
Xing-Long Li,Tao Pan,Jin Deng,Yao Fu,Hua-Jian Xu
Furfural is a biomass-based bulk chemical and its derivatives have potential applications as renewable fuels and chemicals. A water-tolerant and stable solid acid catalyst modified hydrated tantalum oxide (TA-p) was developed for catalytic conversion of D-xylose to furfural in water–organic solvent biphasic system. This process was performed both in a batch reactor and a continuous fixed-bed reactor. In the batch process, D-xylose conversion and furfural yield were significantly affected by the organic solvent, reaction temperature and reaction time. 1-Butanol, which could be obtained through the fermentation of biomass-based carbohydrates, was selected as organic phase and the highest furfural yield of 59% was achieved with D-xylose conversion of 96% at 180 °C in the continuous process. Moreover, the long-time stability test for 80 h under the optimal conditions showed the excellent stability of TA-p catalyst.
研究领域:
生物化学
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催化化学
✓ 已验证
DOI
高影响因子
LAB ON A CHIP
·
2021
·Vol. 21
·期 6
·1109-1117
·
IF 6.799
Fang Wang,Jiaomeng Zhu,Xuejia Hu,Longfei Chen,Yunfeng Zuo,Yi Yang,Fenghua Jiang,Chengjun Sun,Weihong Zhao,Xiaotian Han
Determining the nitrate levels is critical for water quality monitoring, and traditional methods are limited by high toxicity and low detection efficiency. Here, rapid nitrate determination was realized using a portable device based on innovative three-dimensional double microstructured assisted reactors (DMARs). On-chip nitrate reduction and chromogenic reaction were conducted in the DMARs, and the reaction products then flowed into a PMMA optical detection chip for absorbance measurement. A significant enhancement of reaction rate and efficiency was observed in the DMARs due to their sizeable surface-area-to-volume ratios and hydrodynamics in the microchannels. The highest reduction ratio of 94.8% was realized by optimizing experimental parameters, which is greatly improved compared to conventional zinc–cadmium based approaches. Besides, modular optical detection improves the reliability of the portable device, and a smartphone was used to achieve a portable and convenient nitrate analysis. Different water samples were successfully analysed using the portable device based on DMARs. The results demonstrated that the device features fast detection (115 s per sample), low reagent consumptions (26.8 μL per sample), particularly low consumptions of toxic reagents (0.38 μL per sample), good reproducibility and low relative standard deviations (RSDs, 0.5–1.38%). Predictably, the portable lab-on-chip device based on microstructured assisted reactors will find more applications in the field of water quality monitoring in the near future.
研究领域:
环境科学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Lei Cao,Yuanling Li,Peining Li,Xueting Zhang,Lei Ni,Li Qi,Haitao Wen,Xinbo Zhang,Yufeng Zhang
The present study operated the novel moving bed biofilm reactor-nanofiltration-membrane bioreactor (MBBR-NF-MBR) with loose polyamide NF membranes for the first time to treat roxithromycin (ROX) wastewater. Results showed that both MBBR-NF-MBRs achieved superior COD removal of 98.4% and 97.2% and excellent removal of ROX at 74.1% and 65.5%, respectively. The main membrane fouling mechanism was reversible fouling caused by the combination of abundant polysaccharides, proteins and Ca-P precipitates, which could be effectively removed by acidic cleaning. Sorption and biodegradation were the main removal routes of ROX in MBBR . Partial retention of loose NF membrane contributed to microbial metabolism and increased microbial diversity, especially the genera Hyphomicrobium in attached biofilm, which was reasonable for ROX removal. The cleavage of cladinose, demethylation, phosphorylation and β-oxidation in macrolactone ring were the main biotransformation reactions of ROX. This study provides novel insights for micropollutants wastewater treatment by using loose NF membrane in MBR.
研究领域:
环境工程
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废水处理
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Sustainable Chemistry and Pharmacy
·
2023
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IF 6
Chen-Xi He,Yu-Shi Shen,Mei-Yan Ling,Xiao-Hui Yao,Tao Chen,Wei-Guo Zhao,Dong-yang Zhang
Continuous fluid catalysis technology is widely used in industry as traditional fluid reactors have unstable reaction efficiency and poor production conversion. Herein, a continuous fluid reactor with porous directional channel was prepared from natural wood (xylem reactor). When the reactor was used for continuous fluid catalysis, fluid from adjacent channels collided through the pores in the inner walls of the directional channels, creating fluid disturbances that caused full contact between the fluid and enzymes in the reactor. Thus, the porous directional channel structure improved mass transfer and catalytic efficiency, which led to a high biological conversion rate. The xylem reactor exhibited satisfactory catalytic performance and catalyzed the hydrolysis of polydatin at 60 °C for 4 h with an optimum conversion of 99.44%. Additionally, the xylem reactor prevented pressure buildup (0.34 kPa) and displayed cycling stability. The xylem reactor with porous directional channel structure exhibited excellent application potential suitable for sustainable development and green chemistry owing to its low preparation cost, minimal environmental impact and high efficiency.
研究领域:
生物工程
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绿色化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Lulu Wang,Xuhong Guo,Fang Zhao
The luminescent solar concentrator photo microreactor (LSC-PM), a microreactor utilizing luminophores to convert the high-energy photons in sunlight to those that can be absorbed by the photoreaction, holds promising prospects in solar photochemical synthesis, which is to harness sunlight to directly drive photoreactions. Herein, we proposed two new types of LSC-PMs, i.e., the fluoropolymer tube-in-tube reactor and the glass triple-channel reactor, both of which implemented the idea of having luminophores be dissolved in a solvent to form the luminescent fluid, instead of being immobilized in the solid lightguide. The two reactors achieved convenient reuse of the photodegraded luminescent fluid and rapid screening and change of the luminophore types to satisfy the demands of different photoreactions. According to the test of a synthetically significant reaction performed in the triple-channel reactor, it was estimated that a CO2 emission reduction of 15 kg could be realized to produce 1 g of N-formylpiperidine under sunlight as compared to employing an artificial light source. The low-cost and easily assembled tube-in-tube reactor and the deliberately designed triple-channel reactor, with both excellent chemical and irradiation resistance and high flexibility in the choice of luminophore types, could both serve as potential options for different applications in solar photochemical synthesis.
研究领域:
化学工程
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光化学
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可再生能源
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Zhiming Ding,Danfeng Wang,Gang Wang,Runjuan Du,Chuanting Fan,Yaheng Zhang,Jie Zhang,Zhiyong Tang
Electrophotocatalysis, a burgeoning and sustainable technique in the field of organic synthesis, faces challenges in practical implementation due to technological and equipment limitations. This work introduces the design and engineering of an electrophotocatalytic flow microreactor for efficient synthesis of phenol from benzene in one step. The study explored the effects of microreactor configuration and various parameters, such as applied voltage, light intensity, and the concentrations of oxidant DDQ and electrolyte TBABF4. The innovative microreactor, characterized by a compact electrode gap and an expansive optical window, achieved a high phenol yield of 21.2% within a short residence time of 10 min, a significant improvement over the 14.3% yield obtained by a batch reactor in 6 h. The microreactor delivered a remarkable phenol productivity of 116.8 mmol h–1 L–1, outperforming all previous electrophotocatalytic synthesis records. Furthermore, a techno-economic analysis demonstrated that small-scale electrophotocatalytic synthesis of phenol reduced the per-unit production cost, displaying economic advantages competitive with the traditional cumene method. This work provides valuable insights into the engineering of flow microreactors for electrophotocatalysis and offers guidance for the potential industrial-scale application of electrophotocatalytic flow reactions.
研究领域:
有机合成
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化学工程
·
可持续技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Nan Wu,Siming Wang,Ye Yang,Jiayi Song,Ping Su,Yi Yang
A novel type of trypsin capillary microreactor was developed based on a DNA-directed immobilization (DDI) technique applied to a fused-silica capillary modified with polyamidoamine (PAMAM) dendrimers. Trypsin binding to the inner wall of the capillary was confirmed by confocal laser scanning microscopy. The properties of the trypsin–DNA conjugated, PAMAM-modified capillary microreactor were investigated by monitoring hydrolysis of N α-benzoyl- L -arginine ethyl ester. Through the hybridization and dehybridization of the DNA, the inner wall of the capillary functionalized with trypsin can be regenerated, thus indicating the renewability of this enzyme microreactor. In addition, these results demonstrated that introduction of PAMAM enabled higher amounts of trypsin to be immobilized, markedly improving the enzymolysis efficiency, compared with traditional modified capillaries. The digestion performance of the trypsin capillary microreactor was further evaluated by digesting cytochrome C, and a peptide numbers of 8, and a sequence coverage of 59% were obtained. This renewable and efficient immobilized trypsin capillary microreactor combines advantages of both DDI technology and PAMAM, and is potentially adaptable to high-throughput enzyme assays in biochemical and clinical research.
研究领域:
生物化学
·
酶学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Processes
·
2019
·Vol. 7
·期 6
·351
·
IF 1.963
Yali Liu,Xiaorong Kang,Han Cheng
The addition of Fe2+is considered an effective method for increasing methane production, but the added Fe2+may not be absorbed by anaerobic microorganisms due to complex chemical reactions. In this study, ethylenediaminetetraacetic acid (EDTA) was used as a ligand of Fe2+(EDTA-Fe) to promote the dissolution of Fe, and the anaerobic performances of kitchen wastes (KWs) in a semi-continuous reactor were studied. The results indicated that the biogas yields and methane contents were enhanced to 594–613 mL·g−1VSadd·d−1and 63.6–64.4% at an organic loading rate (OLR) of 2.5 gVSadd·L−1·d−1due to EDTA-Fe addition. Simultaneously, the EDTA-Fe was more effective than Fe2+in preventing the acidification of KWs with a high OLR (5.0 gVSadd·L−1·d−1). In addition, the sequential extraction results showed that the water-soluble fraction of Fe in the R3 (EDTA-Fe addition) was 1.49-fold of that in the R2 with Fe2+addition. The contents of coenzymes F420 and F430 were also improved 1.09 and 1.11 times, respectively. Mechanism analysis confirmed that the EDTA enhanced methane production and operational stability by promoting the dissolution of Fe and maintaining a high content of water-soluble Fe.Keywords:semi-continuous reactor;EDTA;Fe;kitchen wastes;methane
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Flow Chemistry
·
2018
·Vol. 8
·期 3
·139-146
·
IF 1.658
Liu Yang,Zeng Changfeng,Wang Chongqing,Zhang Lixiong
We design a new continuous diazotization microreaction process in which concentrated acid and sodium nitrite solutions are used. We form oil in water (O/W) microdroplets by mixing amine with sodium nitrite aqueous solution, which react with mineral acid to make the diazotization. In this way, the formed particles are dispersed in the aqueous phase, which can flow with the main stream, and consequently are consumed. Then the fluid can smoothly flow without clogging in the microchannels and we can achieve continuous operation. We use aniline, o-methylaniline, m-methylaniline, and o-ethylaniline as the raw materials, 6 M HCl and 6.5 M H 2 SO 4 as the acid as well as sodium nitrite to demonstrate the feasibility. The resulting diazonium salts are converted to iodo-substitute aromatic compounds to measure the yield. The results show that, SIMM-V2 micromixer is efficient to form the emulsion. Mixing the microdroplets with acid in a co-axial flow way can prevent clogging. In hydrochloric acid medium, high reaction yield can be obtained when the reaction temperature is 4 °C and the residence time is 13–15 s. In sulfuric acid medium, high conversion of aromatic amine of up to 99% and yield of approximately 90% can be achieved at the reaction temperature of 9 °C and the residence time of 14–16 s. This process provides efficient continuous diazotization reactions using concentrated acid and sodium nitrite solutions, minimizing the production of waste water.
研究领域:
化学工程
·
有机合成
✓ 已验证
DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2022
·
IF 8.129
Huiyuan Wang,Tan Li,Jing Su,Kai Miao,Kaige Wang
Lignin hydropyrolysis was investigated in a high-pressure micro-reactor with on-line analysis characterization of products. The influences of atmosphere, pressure, and temperature on product formation during lignin pyrolysis were quantitatively discussed. The presence of hydrogen, elevated pressure and temperature favored the production of condensable vapors and permanent gas while inhibited the formation of char. High-pressure hydrogen boosted the conversion of methoxy phenolics to non-methoxy phenolics, and facilitated the generation of hydrocarbon condensable vapors and hydrocarbon gases. Non-methoxy phenolics were major condensable vapor from lignin hydropyrolysis. Yield of non-methoxy phenolics reached 19.68C% at 3 MPa and 500 °C. The monocyclic and polycyclic aromatic hydrocarbons as well as condensable aliphatic hydrocarbons were observed from lignin hydropyrolysis. Methane was the most abundant gas product from lignin hydropyrolysis, yield of which was 10.27C% at 2 MPa and 600 °C. The formation mechanisms of typical products were proposed and verified with experiments using model compounds. The formation of non-methoxy phenolics, monocyclic aromatic hydrocarbons and aliphatic hydrocarbons is related to the substitution of methoxy (and its methyls) and phenolic hydroxyl groups on benzene rings by alkyls, alkenyls and hydrogen radicals, as well as the hydrogenation of π bonds .
研究领域:
化学工程
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）