Yu Li,Yejing Wang,Kai Song,Lina Liu,Rui Cai,Gang Tao,Ping Zhao,Haimeng Zhou,Huawei He
Adenosine kinase (ADK) plays an important role in the growth and development of organisms. A convenient, quick, reliable, sensitive and low-cost assay for ADK activity is of great significance. Here, we found the reaction system with bromothymol blue as the pH indicator had a maximum absorption peak at 614 nm. The absorbance change in 614 nm was positively correlated with the generated hydrogen ions in the reaction catalyzed by ADK. Then, we demonstrated this assay was feasible for ADK activity. Further, we analyzed the effects of buffer, bromothymol blue concentrations on the sensitivity of the assay, and investigated the sensitivity of ADK contents and adenosine concentration on the assay. Finally, we calculated the K m and Vmax of ADK from Bombyx mori with this assay. Our results suggested this assay was quick, convenient, reliable, sensitive and economic for the activity of ADK. It is an excellent alternative for the conventional ADK assays.
Miao Xu,Shen Wang,Shengyu Zhou,Wenhai Sun,Zhenmin Ding,Xiang Zhang,Jiupeng Zhao,Yao Li
In this study, a novel TiO 2 @C@Prussian Blue core-shell nanorod arrays film was constructed through two-step hydrothermal and electrodeposition methods. Such a designed structure could increase the specific surface area, reactive site, and conductivity of Prussian Blue (PB) and reduce the diffusion distance of ions due to the nano-architecture arrays of TiO 2 and the high conductivity of the carbon layer, thus leading to the remarkably improved storage capacity and reaction speed of ions during the electrochromic (EC) process of PB. Moreover, porous morphology and strong adhesion on the FTO substrate of the TiO 2 nanorod arrays could alleviate the lattice stress and volume expansion and enhance the binding force, thus improving the cycle stability of EC. Given these benefits, the TiO 2 @C@PB films display a large optical modulation range (67%), fast switching speed (t c /t b = 1.25 s/2.71 s), and high coloration efficiency (129.4 cm 2 /C). In particular, the EC cycle stability is remarkably improved (96.3% after 5000 cycles), implying a promising application in high-performance EC devices (ECDs).
危险品化学品经营许可证（带存储）