Food Bioscience
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2026
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IF 6.2
Jingwen Li,Hossain M. Zabed,Yufei Zhang,Junhua Yun,Yuehui Tian,Guoyan Zhang,Xianghui Qi
Liquiritigenin (LIG) is a natural flavonoid with diverse bioactivities, rendering it attractive for applications in food, pharmaceuticals, nutraceuticals, and cosmetics. However, its de novo biosynthesis in microbial hosts faces several major challenges, including the lack of efficient enzymes compatible with heterologous systems, insufficient supply of precursor amino acids, suboptimal cofactor availability and electron transfer efficiency. To evaluate these constraints, Saccharomyces cerevisiae was engineered through systematic stepwise comparison of p -coumaric acid-forming routes, downstream enzyme sources, aromatic amino acids precursor supply, NADPH availability, and fermentation conditions. The best-performing strain QL14 produced 4.09 mg/L LIG in shake-flask fermentation and 8.42 mg/L LIG in 5-L fed-batch fermentation. However, naringenin (NAR) remained the predominant downstream product. These results indicate that increased pathway input improved overall flavanone output but was insufficient to redirect flux toward the CHR-dependent LIG-forming branch. Therefore, downstream branch selectivity, rather than precursor or NADPH supply alone, represents the major constraint in the current yeast system. This work provides a basis for future CHR-focused engineering and controllable LIG/NAR co-production.
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FOOD SCIENCE AND BIOTECHNOLOGY
·
2026
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IF 3.9
Liu Yating,Zhong Jie,Gan Na,Chen Lijuan,Liu Peiran,Xu Junjie,Geng Fang,Li Tingting,Wu Di
To tackle the high volatility, hydrophobicity and instability that limited cinnamaldehyde (CMA) in practical applications, CMA nanoemulsion (CNE) synergistically stabilized by cinnamic acid (CA) and Tween-80 were successfully prepared and characterized by ultrasonic emulsification treatment. The results showed that the CNE particle size was uniform (105.54 ± 2.14 nm). The CMA in CNE showed better slow-release properties under sealed conditions (22.68 ± 2.81% on day 10). The heat stability, freeze–thaw stability and storage stability of CNE showed good stability during 10 d storage period. Sensory and physicochemical property analyses indicated that the CNE was released headspace under non-contact conditions, reducing the impact on the flavor of chilled beef. It also effectively mitigated the increase in pH, juice loss and the increase in total volatile base nitrogen (TVB-N), thiobarbituric acid-reactive substance (TBARS), and total number of microbial colonies (TNC) of chilled beef during refrigeration. Graphical abstract
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LANGMUIR
·
2026
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IF 3.9
Meikun Hu,Jiaming Feng,Haoqian Wang,Zhihang Wang,Nong Wang
Stimulus-responsive emulsions and gels are powerful platforms for intelligent soft materials, yet the reversible light-controlled modulation of their macroscopic properties has remained elusive. Here we report a multicomponent aqueous formulation─comprising cetyltrimethylammonium bromide (CTAB), cosurfactants, oil, water, and para-aminoazobenzene (AAB)─that, depending on water content, enables robust and reversible control of both viscoelasticity and emulsion stability with light. Within a defined composition window, the system forms entangled wormlike micelles that impart gel-like viscoelasticity. Ultraviolet irradiation disrupts this network, reducing viscosity by orders of magnitude, while visible light restores the original structure. At higher water content, the same formulation produces stable oil-in-water emulsions whose integrity can be reversibly switched by photoisomerization-driven interfacial rearrangements. This dual-level responsiveness demonstrates a simple and general strategy to couple molecular photoisomerization with macroscopic flow and phase behavior, opening new opportunities for adaptive formulations, reconfigurable soft matter, and light-controlled delivery technologies.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 5
·2247-2257
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IF 3.3
Jiao Wu,Bingjun Han,Xiaoyu Chen,Jinglin Gao,Shan Zhao,Liping Sun,Shijie Wang
Summary Stingless bee honey is a precious food product with therapeutic benefits. In this study, we determined the quality of Malaysian stingless bee honeys ( Heterotrigona itama and Tetrigona binghami ) by the physicochemical characteristics, phenolic compounds, and organic acids. Our results revealed that the major phenolic compounds in all honey samples were chlorogenic acid and myricetin, and the major organic acids contained gluconic acid and trans -cinnamic acid. The microbial community in honeys characterised using 16S rRNA and ITS amplicon sequencing techniques showed that Lactobacillus was assumed to be the predominant bacterium in stingless bee honeys. Furthermore, the antibacterial activity of honeys was examined in order to further demonstrate their health benefits. Our results are intended to pave the road for stingless bee honeys' probiotic capacity to be extended into our daily life.
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ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Yaping Li,Ningning Sun,Songxin Ma,Xin Zhang,Yingfeng Wang,Xingru Li
Magnetic thermo-responsive branched polymer (Fe 3 O 4 @poly(glycidyl methacrylate)@poly( N -isopropylacrylamide)) was fabricated for the first time and applied for microwave-assisted magnetic solid phase extraction of phenolic acids in olive oil samples followed by ultra-high performance liquid chromatography-tandem mass spectrometry analysis in multiple reaction monitoring mode. Owing to the controllable molecular weight of poly(glycidyl methacrylate) synthesized by atom transfer radical polymerization and the thermo-responsive characteristic of poly( N -isopropylacrylamide), extraction performance could be efficiently tuned and enhanced. The whole sample pretreatment process was accomplished within 1 min with the help of the microwave. The nanocomposites were characterized by transmission electron microscope , scanning electron microscope, Fourier transform infrared spectroscopy , thermogravimetric analysis , vibrating sample magnetometer, water contact angles and dynamic light scattering . The adsorption experimental data fitted well with the Freundlich isotherm model and followed the pseudo-second-order kinetic model . The factors affecting the extraction process including adsorbent amount, adsorption time, sample volume, desorption conditions and interferents were investigated and optimized. Under the most favorable conditions, the developed method showed good linearity (R 2 ≥ 97.98%) in the range of 0.2–30 μg L −1 , low limits of detection (0.005–0.030 μg L −1 ) and limits of quantification (0.016–0.098 μg L −1 ) as well as satisfactory precision (RSDs≤4.85%). Our proposed method was successfully used for determination of phenolic acids in olive oil samples and satisfactory recoveries at three spiked concentration levels were in the range of 84.6–108.1% with RSDs less than 9.20%. Coupled with principal component analysis , our developed method proved promising for fast and convenient differentiation between extra virgin olive oils and refined olive oils.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 20
·12365
·
IF 6.208
Xiang Zhou,Yi-Hong Fu,Ya-Yu Zou,Jiao Meng,Gui-Ping Ou-Yang,Qiang-Sheng Ge,Zhen-Chao Wang
To develop novel microtubule-binding agents for cancer therapy, an array ofN-cinnamoyl-N’-(substituted)acryloyl hydrazide derivatives were facilely synthesized through a two-step process. Initially, the antiproliferative activity of these title compounds was explored against A549, 98 PC-3 and HepG2 cancer cell lines. Notably, compoundI23exhibited the best antiproliferative activity against three cancer lines with IC50values ranging from 3.36 to 5.99 μM and concurrently afforded a lower cytotoxicity towards the NRK-52E cells. Anticancer mechanism investigations suggested that the highly bioactive compoundI23could potentially promote the protofilament assembly of tubulin, thus eventually leading to the stagnation of the G2/M phase cell cycle of HepG2 cells. Moreover, compoundI23also disrupted cancer cell migration and significantly induced HepG2 cells apoptosis in a dosage-dependent manner. Additionally, the in silico analysis indicated that compoundI23exhibited an acceptable pharmacokinetic profile. Overall, these easily preparedN-cinnamoyl-N’-(substituted)acryloyl hydrazide derivatives could serve as potential microtubule-interacting agents, probably as novel microtubule-stabilizers.Keywords:cinnamic acid derivatives;antiproliferative activity;microtubule;tubulin stabilizer
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JOURNAL OF TEXTURE STUDIES
·
2022
·Vol. 53
·期 5
·662-671
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IF 3.942
Xuehui Cao,Ge Bai,Jianrong Zheng,Danshi Zhu,Jianrong Li
Freezing is a way to preserve the quality of fruit for a long time. Nanguo pear stored at low temperature is prone to browning and lignification. In this study, freeze–thaw cycles were used to simulate the temperature fluctuation in the process, storage, and transportation. The texture properties were taken as the research focus to analyze the lignification phenomenon of Nanguo pear under freeze–thaw cycles. The results showed that freeze–thaw treatment significantly reduced the firmness and propectin content of Nanguo pear, increased the content of stone cells in the fruit, but also destroyed the size of stone cells in the fruit. However, with the increase of freezing–thawing cycles, the content of lignin, stone cell content, and PAL activity increased significantly, while the content of chlorogenic acid increased first and then decreased. These results are helpful to further understand the correlation between texture change with fruit firmness and formation mechanism of stone cells during freeze–thaw cycles of Nanguo pear.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Jing Wang,Mengru Li,Hui Wang,Wenjing Huang,Fang Li,Lili Wang,Chi-Tang Ho,Yanyan Zhang,Liang Zhang,Xiaoting Zhai,Xiaochun Wan
Aroma extract dilution analysis was performed on volatile fractions extracted from a freshly prepared Dahongpao (DHP) tea infusion using solvent-assisted flavor evaporation, yielding 65 odor-active domains with flavor dilution factors ranging between 32 and 32,768. In addition, six aromatic substances were captured by headspace analysis. Quantitation of 54 compounds by an internal standard method and stable isotope dilution assays revealed that the concentrations of 32 odorants exceeded their respective orthonasal odor threshold values in tea infusion. The results of odor activity values (OAVs) suggested that 2-metylbutanal (malty) and γ-hexalactone (coconut-like) had the highest OAVs (248 and 154). Eight odorants including γ-hexalactone (OAV 154), methyl 2-methylbutanoate (59), phenylacetic acid (7.2), acetylpyrazine (5.7), 2-methoxyphenol (3.4), p-cresol (2.7), 2,6-diethylpyrazine (2.7), and vanillin (1.8) were newly identified as key odorants in DHP tea infusion. An aroma recombination model in a non-volatile matrix extracted from tea infusion satisfactorily mimicked the overall aroma of DHP tea infusion, thereby confirming the identification and quantitative experiments. Omission experiments verified the obvious significance of 6-methyl-5-hepten-2-one (OAV 91), 2-ethyl-3,5-dimethylpyrazine (19), 4-hydroxy-2,5-dimethylfuran-3(2H)-one (13), and acetylpyrazine (5.7) as key odorants for the special roasty and caramel-like aroma of DHP tea.
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Ya zhu Wang,Jin Zheng,Muhammad Nawaz,Fan Yang,Jiajun Hu,Min-Tian Gao
In this study, it was found that soluble polysaccharides (SPs) from rice straw were conjugated with high levels of phenolic compounds . A gradient ethanol precipitation method was used to separate conjugates with different phenolic compound components. These conjugates were mainly composed of disaccharides or trisaccharides and phenolic acids that were mainly p- coumaric acid, ferulic acid, vanillic acid, and vanillin. SPs with more conjugates exhibited better effects on Saccharomyces cerevisiae and ethanol fermentation . The change in the proportion of conjugates in SPs significantly affected the accumulation of intracellular trehalose and glycerol content, indicating the effect of the conjugates on intracellular osmotic stress. Moreover, SPs with more conjugates generated lower intracellular hydrogen peroxide (H 2 O 2 ) levels. Thus, oligosaccharide and phenolic compound conjugates in SPs promoted yeast growth and ethanol fermentation. For instance, OD value reached 9.4, which was 1.3 times higher than the control group, and ethanol content at 36 h was 36.5 g/L, which was 1.3 times higher than the control. Osmotic pressure alleviation and lowered intracellular H 2 O 2 levels by conjugates are key mechanisms of the SPs during yeast growth and ethanol fermentation.
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yuan Deng,Kai Li,Qingqing Guan,Tianding Hu,Liang He
The improvement of the photothermal stability and mechanical properties for cellulose nanofibers (CNFs) is a highly desirable yet challenging research topic due to its wide applications. Featuring the 2D-organized structure and various characteristics, layered double hydroxides (LDHs) shows tremendous potential in improving the photothermal stability and mechanical properties of CNFs matrix. Herein, a series of ZnAl-LDHs nanosheets intercalated with different organic molecules were synthesized and subsequently doped into CNFs matrix for preparing CNFs/LDHs composited films. It was showed that doping UV-adsorber intercalated ZnAl-LDHs into the CNFs matrix can significantly enhance UV-shielding ability of CNFs/LDHs composited films due to the synergistic effect between organic anions and the interaction between organic anions and metal cations in LDHs laminates. And the CNFs/HUL (HUL = HALS/UV-P-LDHs) composited film exhibited outstanding UV-shielding ability (UV-shielding rate > 60%) among the considered films, which is comparable to that of the reported materials. Compared with the pristine CNFs film, compositing with HUL rendered the increasing of tensile strength from 80 to 110 MPa. The superior photothermal stability and mechanical properties of CNFs/HUL composited film highlight its great application potential in the fields of coating and packaging.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Rongkun Zhu,Zengcheng Du,Mingfang Zhu,Huanru Liang,Shumei Wang,Qing Zhou,Rui Li,Yulan Li,Chaoying Zeng,Wenhao Liu,Qiqing Chen
A ratiometric fluorescence sensor based on molecularly imprinted polymers embedded with double perovskite quantum dots (green fluorescent CsPbBr 3 (GPQDs) and red fluorescent CsPbI 3 (RPQDs)) were prepared for the visible and fluorescent determination of Rhein. Double perovskite quantum dots were synthesized by a one-pot method with in-situ hydrolysis, and the fluorescent probes containing GPQDs and RPQDs with molecularly imprinted cavities (MIPs@RPQDs/GPQDs@SiO 2 ) were synthesized by sol gel method. And the synthesized MIPs@RPQDs/GPQDs@SiO 2 can emit two stable and independent fluorescence peaks at 520 nm with green fluorescence and at 695 nm with red fluorescence respectively. Since Rhein could specifically quench the red fluorescence without affecting the green fluorescence at 520 nm, a ratiomitric fluorescence sensor could be and was constructed with GPQDs as the internal standard signal and the RPQDs as the response signal. The sensor had a good linear relationship with the concentration of Rhein between 15 and 135 nM, and the detection limit was 1.90 nM (S/N = 3). Moreover, Rhein could be detected by observing the color transformation of the fluorescent probe directly with naked eyes. In addition, Rhein in the traditional Chinese medicine Rheum palmatum L. had been detected by the constructed ratiometric fluorescene probe with satisfactory.
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JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Yuandong Yu,Guizhao Liang
Zein, a mixture protein existed in maize endosperm, has desirable biocompatibility and bioadhesion, which has been extensively applied as carrier materials in food and pharmaceutical industry. However, to the best of our knowledge, there is no published paper that has systematically explored the binding between phenolic acids and zein on the basis of the regression model. In this study, we investigated interaction behaviors of 19 phenolic acids with zein via fluorescence spectroscopy and partial least squares regression (PLS) modeling, among which trans -ferulic acid (hydroxycinnamic acid with the highest pK A values) and 3,5-dinitrosalicylic acid (hydroxybenzoic acid with the lowest pK A values) were selected to further investigate their interaction mechanisms based on molecular modeling methods. The fluorescence spectra results showed that these 19 phenolic acids can cause obvious fluorescence quenching of zein, and the binding capacities of hydroxycinnamic acid were generally better than those of hydroxybenzoic acid. Molecular modeling results exhibited that the hydrogen bonding interaction and van der Waal force played crucial roles in maintaining the stable binding of these complexes; the formation of hydrogen bonds between Tyr-192 and carboxyl group on hydroxycinnamic acid was suggested to be the critical interaction causing stronger fluorescence quenching. The PLS modeling revealed that molecular descriptors P1s, Ks, ASP, Km, L/BW, P1m, JGI7, P1e, P1v, X3A had positive effects to the pK A of 19 phenolic acids, while P2s, P2m, L2s, PW4, JGI3 had negative effects. These findings suggest that zein should have the potential application as the polyphenol delivery system, and provide information for engineering zein to become more efficiently polyphenol carriers.
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