FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jing Wei,Jingtao Cui,Yanchi Zhou,Tong Li,Qiaozhu Wei,Tian Li,Weimin Zhang
The effects of cis – trans isomerization on the oxidation products of oleic acid (composites and structure characterization) were investigated. The reduction of thermal oxidation reaction of methyl elaidate (ME) was 6.72 % lower than that of methyl oleate (MO), and the oxidation products (core aldehydes) first increased and then decreased with the prolongation of thermal oxidation time. ME exhibited better oxidation stability than MO in free radicals, hydroperoxides, double bonds, aldehydes, and glycerides. The oxidation products were mainly 11-oxo-9-undecenoate (initial stage) and methyl 9-oxononanoate (later stage), following the free radical chain reaction mechanism. The H8 in double bond was more easily dehydrogenated in the chain initiation stage than H11, followed by the formation of core aldehyde through three intermediates and two transition states each pathway. ME required a higher energy barrier (1.3–13.6 kJ/mol) than MO reaction, making it more difficult for ME to undergo thermal oxidation reaction. The transition state 4 and 5 were rate determining steps of chain initiation reaction and proliferation reaction, respectively. This study was of great significance to further control the formation of these trans fats and their oxidation products.
研究领域:
食品科学
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化学动力学
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脂质氧化
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DOI
PubMed
高影响因子
CyTA-Journal of Food
·
2018
·
IF 1.371
Yingmei Wu,Wenfeng Li,Yalong Lu,Qiu Wu,Xingbin Yang
High fructose (HF) ingestion is a common risk factor for the development of nonalcoholic fatty liver disease (NAFLD), which has become a serious health problem. The underlying mechanism of HF-induced NAFLD needs to be further understood and an effective prevention needs to be established urgently. Gas chromatography-mass spectrometer-based metabonomic method was employed to characterize the serum metabolic profile of HF-induced NAFLD in mice. β-Hydroxybutyric acid, elaidic acid and oleic acid that contributed to energy and lipid metabolism were confirmed as potential biomarkers for HF-induced NAFLD. With these changed metabolic pathways as possible drug targets, different administrations revealed that co-administration of stachyose and tea polyphenols was a more effective method than treatment of stachyose or tea polyphenols alone for preventing HF-induced negative changes of metabolic pathways in mice. These findings suggest that β-hydroxybutyric acid, elaidic acid and oleic acid are potential biomarkers in HF-induced NAFLD, and co-administration is a novel preventive strategy.
研究领域:
营养学
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代谢组学
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药理学
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生物化学
·
食品科学
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Jing Lu,Ziheng Chen,Xiujuan Bu,Shanshan Chen,Shuang Guan
Elaidic acid (EA, C18:1 trans) is a kind of principal Trans fatty acid (TFA) and is widely found in processed food. Pyroptosis is a form of programmed cell death , distinct from apoptosis and traditional necrosis. Excessive pyroptosis could induce body injury and serious inflammation. However, the effect of EA on pyroptosis has not been reported. In the study, we found that EA exposure caused liver damage and hepatocyte pyroptosis by testing GSDMD-N, Caspase 1 , IL-18, and IL-1β in mice and HepG2 cells. Further exploring the mechanisms, we found that EA-induced pyroptosis depended on Cathepsin B (CTSB)-mediated NLRP3 inflammasome activation. Cell autophagy was closely related to lysosomes . Our study revealed that EA promoted hepatocyte autophagy, and activated autophagy induced lysosomal membrane permeabilization (LMP) and CTSB leakage . Inhibition of autophagy by 3-MA mitigated the CTSB leak, reduced the activation of the NLRP3 inflammasome, and then attenuated the EA-induced pyroptosis. In summary, these results indicated that EA induced hepatocyte pyroptosis via autophagy-CTSB-NLRP3 inflammasome pathway. The study revealed new insights into the toxicity mechanism of EA.
研究领域:
毒理学
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细胞生物学
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分子生物学
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肝病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2023
·
IF 5.4
Dong-mei Li,Fu-cui Zhu,Jie Wei,Jia-xiu Xie,Jun-hui He,Dong-mei Wei,Yi Li,Ke-dao Lai,Li-min Liu,Qi-biao Su,Gui-ning Wei,Bin Wang,Yan-cheng Liu
Ethnopharmacological relevance Polyrhachis vicina Roger ( P. vicina ), a traditional Chinese medicinal animal, has been used to treat rheumatoid arthritis , hepatitis, cancer, and other conditions. Due to its anti-inflammatory properties, our previous pharmacological investigations have demonstrated that it is effective against cancer, depression, and hyperuricemia . Nevertheless, the key active components and targets of P. vicina in cancers are still unexplored. Aim of the study The study aimed to evaluate the pharmacological treatment mechanism of the active fraction of P. vicina (AFPR) in treating colorectal cancer (CRC) and to further reveal its active ingredients and key targets. Methods To examine the inhibitory impact of AFPR on CRC growth, tumorigenesis assays, cck-8 assays, colony formation assays, and MMP detection were utilized. The primary components of AFPR were identified by GC-MS analysis. The network pharmacology , molecular docking, qRT-PCR, western blotting , CCK-8 assays, colony formation assay, Hoechst staining, Annexin V-FITC/PI double staining, and MMP detection were performed to pick out the active ingredients and potential key targets of AFPR. The function of Elaidic acid on necroptosis was investigated through siRNA interference and the utilization of inhibitors. Elaidic acid's effectiveness to suppress CRC growth in vivo was assessed using a tumorigenesis experiment. Results Studies confirmed that AFPR prevented CRC from growing and evoked cell death. Elaidic acid was the main bioactive ingredient in AFPR that targeted ERK. Elaidic acid greatly affected the ability of SW116 cells to form colonies, produce MMP, and undergo necroptosis. Additionally, Elaidic acid promoted necroptosis predominantly by activating ERK/RIPK1/RIPK3/MLKL. Conclusion According to our findings, Elaidic acid is the main active component of AFPR, which induced necroptosis in CRC through the activation of ERK. It represents a promising alternative therapeutic option for CRC. This work provided experimental support for the therapeutic application of P. vicina Roger in the treatment of CRC.
研究领域:
药理学
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肿瘤学
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传统中药
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Bo Zheng,Hui Wen,Xingwei Xiang,Bin Zheng
This study elucidates how dynamic high-pressure microfluidization (DHPM) synergistically combined with ultrasound (US) pretreatment regulates the molecular assembly, hierarchical structure, and in vitro digestive behaviors of starch-unsaturated fatty acid (UFA) complexes. Fully gelatinized wheat starch (WS) was complexed with trans-oleic acid (EA)/linoleic acid (LA) and processed by US-assisted DHPM. Distinct digestion behaviors were observed, with WS-LA complexes exhibiting a significantly higher resistant starch (RS) content up to 21% than the EA counterparts. Structural analyses revealed that US pretreatment enhanced amylose chain mobility and promoted the formation of single-helical inclusion structures, while subsequent DHPM intensified starch-lipid interactions and drove the self-assembly of compact supramolecular domains. Owing to its near-linear conformation, EA favored deeper helix insertion and higher V -type crystallinity, whereas LA was associated with fewer but potentially more densely organized nanostructures. Correlation analysis demonstrated that digestive resistance and release rate was governed not only by the extent of inclusion complexation, but more critically by the hierarchical organization of assemblies. These findings provide mechanistic insights into structure-digestibility relationships and offer guidance for designing starch-based systems with tailored digestion properties.
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Bo Zheng,Hui Wen,Xingwei Xiang,Bin Zheng,Tianjie Li
UFA molecular geometry directs hierarchical assembly of starch-lipid systems. • US-DHPM induces UFA-templated starch reassembly from helices to aggregates. • C18 UFAs promote ordered lamellar barriers and enhanced digestion resistance. • Long-chain PUFAs generate strain-induced heterogeneity and rapid digestion. • Digestive resistance is governed by barrier continuity rather than CI alone. The influence of unsaturated fatty acid molecular geometry on the assembly and digestive properties of starch-lipid complexes remains unclear. Here, five UFAs with distinct chain lengths and configurations were incorporated into gelatinized wheat starch via ultrasound-assisted dynamic high-pressure microfluidization to construct hierarchical complexes. Multiscale analyses showed that UFA geometry governed amylose helix inclusion, V-type crystallinity, and lamellar packing. Monounsaturated UFAs promoted compact ordered assemblies, whereas highly unsaturated UFAs generated looser interfacial structures. These differences were reflected in digestion behavior, with oleic-acid-based complexes exhibiting enhanced digestion resistance and delayed lipid release, while certain highly unsaturated systems such as DHA showed accelerated starch hydrolysis and fatty acid liberation. Notably the linoleic acid-based complex exhibited the highest resistant starch content of 20.77% and the slowest fatty acid release with 29.70% of fatty acids still retained after 120 min of digestion. Correlation analysis and molecular dynamics simulations further indicated that digestive resistance depends on the continuity of hierarchical packing rather than complexation efficiency alone. These findings clarify geometry-dependent structure-digestion relationships in starch-lipid complexes and support structure-guided design of systems with tunable digestive properties. Download: Download high-res image (203KB) Download: Download full-size image
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产品关联
DOI
高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Yibo Zhang,Yang Liu,Wei Wang,Chunlai Liu,Hongyu Zhou,Na Wang,Jianglei Hu,Fengwei Shi
Metal-organic framework (MOF)-based composites have received significant attention for catalytic applications and are believed excellent heterogeneous catalysts. In this article, we prepared a new type of material, HPW@MOF-199/SBA-15C (PMS-15C). For this material, the MOF-199 encapsulating Keggin-type phosphotungstic acid (HPW) was grown inside the pores of the SBA-15 functionalized by carboxylic acids. The N 2 adsorption-desorption isotherms and pore size distributions, X-ray powder diffraction, transmission electron microscopy, and scanning electron microscopy analyses showed that the PMS-15C composites had an ordered two-dimensional hexagonal mesoporous structure, in which HPW encapsulating by MOF-199 loaded into the SBA-15C. Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy indicated that HPW was successfully loaded, and the Keggin structure of HPW in PMS-15C remained unchanged. With the reaction of acetic acid and ethanol, the catalytic performance of PMS-15C was studied. Under the optimal conditions, ethyl acetate yield reached 81.4 %. The forward reaction rate was 0.432 mL mol −1 min −1 , and the activation energy was 38.35 kJ mol −1 . And the data were found to be consistent with the second-order reversible reaction kinetic model. The catalytic activity of the PMS-15C-0.2 composites remained stable after five catalytic cycles. In addition, the esterification of n-octanol/acetic acid and ethanol/oleic acid were studied using PMS-15C-0.2 as the catalyst. The results suggested that the catalytic activity of PMS-15C-0.2 was weakened upon increasing the substrates’ alkyl chain length.
研究领域:
材料科学
·
催化化学
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产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Bin Wang,Kai Gao,Yujie Wang,Qiuying Chang,He Yang
Magnesium silicate hydroxide (MSH), a self-healing agent at the nanoscale, is a potential novel additive material for lubricating oil. This study explores the impact mechanism of dispersibility and absorptivity on MSH’s anti-wear performance, using experimental research and molecular dynamics simulations. Results show that MSH alone, as an additive to poly-alpha-olefin lubricating oil, does not exhibit anti-wear effect. However, MSH modified by carboxylic acids, including oleic acid, elaidic acid, or stearic acid, reduces the wear by 44.1%, 31.5%, and 21.0%, respectively. This reduced wear is a result of improved dispersibility of MSH and enhanced adsorption on iron interface. Carboxylic acid connects to the hydroxyl groups of the Mg-O/OH tetrahedron through hydrogen bonds and exist in a form where the alkyl chains deviate from the solid interface. In comparison to elaidic acid and stearic acid, oleic acid can form a denser modified layer on the surface of both MSH and iron, resulting in superior dispersibility of MSH in lubricating oil and stronger adsorption on iron surfaces, ultimately leading to better anti-wear performance.
研究领域:
材料科学
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润滑工程
·
表面化学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Tianli Guo,Hongrui Hou,Yufan Liu,Ling Chen,Bo Zheng
This study emphasized on structural alteration of rice starch-unsaturated fatty acid complexes by adding trans-2-dodecaenoic acid (t12), trans-oleic acid (t18), cis-oleic acid (c18) and linoleic acid (loa) with different concentration under high-pressure homogenization treatment, and further illustrated the underlying mechanism of modulating digestibility. Results showed that the complex primarily presented as V6 or type IIa polymorph; complex index, content of ordered structure and thermal stability appeared to be positively correlated to the concentration of unsaturated fatty acids. t12 was too mobile to form single helix, leading to the formation of loose matrix; t18 fitted better within the cavity of starch than c18, and formed structural domain with higher compactness and thermal stability; Rloa had lower complex index but higher degree of short-range order, and tended to form alternating amorphous and crystalline structure. The digestibility was higher in the order of Rloa, Rt18, Rc18 and Rt12.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2019
·Vol. 9
·期 13
·3380-3387
·
IF 5.726
Xiaochao Zhang,Jiabing Wei,Guangmin Ren,Changming Zhang,Zhanfeng Zheng,Caimei Fan
This work reported for the first time a systematic investigation on a BiOBr photocatalyzed oleic acid isomerization process in different (air, N2 and O2) atmospheres and proposed the cis–trans isomerization mechanism to effectively restrain the formation of trans fatty acids with deleterious effects on human health. The phase structures, optical properties, and morphologies of as-prepared BiOBr were characterized by XRD, UV-vis DRS, SEM and TEM. Besides, the photocatalyzed isomerization products over BiOBr in different atmospheres were analyzed with FT-IR and 1H NMR for comparison. The results demonstrate that there are significant differences in the photocatalytic isomerization process of oleic acid in different atmospheres, and especially the N2 atmosphere is the most favorable atmosphere, while the presence of O2 will inhibit the formation of elaidic acid. Moreover, density functional theory (DFT) was employed to calculate the charge density and energy difference of ground states between cis- and trans-isomers of oleic acid. Combining the theoretical and experimental findings, the radical cation and photo-induced h+ action mechanisms in the photocatalytic isomerization process were discussed and proposed. Our findings should provide new insights and basic data for understanding the photocatalytic isomerization of unsaturated fatty acids.
研究领域:
食品科学
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化学工程
·
生物化学
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产品关联
DOI
高影响因子
SENSORS
·
2018
·Vol. 18
·期 10
·3252
·
IF 2.475
Feng Zhang,Xiaojie Wang,Xu Jie,Weili Wei
Fatty acids (FAs) are of interest to the areas of food science and medicine because they are important dietary sources of fuel for animals and play important roles in many biological processes. The health effects of FAs are different due to the diversity of olefinic bonds in the alkyl chains including number, position and configuration. However, the discrimination of FAs is difficult from a chemical sensing perspective due to the lack of diversity in terms of functional groups. Until now, only a few chemosensors have been developed for selective sensing of FAs based on their overall shape, however they are still limited in discrimination of FAs with subtle structural differences, moreover, they cannot be used for rapid and in situ inspections. Herein, for the first time, we designed a test paper for in situ colorimetric inspection for FAs based on the combination of the highly selective binding of Ag+to olefinic bonds and Ag+mediated color variation of 3,3′,5,5′,-tetramethylbenzidine. As a result, the sensor exhibited high sensitivity and good selectivity for five FAs with subtle structural differences. Furthermore, our method described herein was successfully applied to monitor the structural variations of FAs and quality changes in mixture edible hot pot oils with heat treatment in time course. Hence, the test paper presented herein holds great potential in the inspection of fats and edible oils in food industries.Keywords:test paper;colorimetric sensor;fatty acid;edible oil
研究领域:
食品科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Jingqing Wu,Ying Ge,Weidong Qin
A method was developed for determining free fatty acids in edible plant oils by incorporation of running-buffer-mediated liquid–liquid extraction and polyamidoamine-dendrimer-assisted capillary electrophoresis–capacitively coupled contactless conductivity detection. The recoveries for the extraction were in the range of 90.1% and 110.3%. Addition of dendrimer to the running buffer improved the separation of fatty acids. Under the optimized buffer conditions, i.e., 3 mM pelargonic acid, 39 mM tris(hydroxymethyl)aminomethane, 30 mM polyoxyethylene 23 lauryl ether, 35% acetonitrile, 15% 2-propanol, 2.5% 1-octanol, and 300 μM polyamidoamine generation 2 at apparent pH 8.53, the 10 model fatty acids were separated in 18 min with detection limits ranging from 0.46 to 3.28 μM. The successful determination of fatty acids in real samples suggests that the method is simple, cost-effective, and easy to operate and is suitable for scanning free fatty acid in edible plant oils.
研究领域:
分析化学
·
食品科学
·
电泳技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）