JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
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IF 2.8
Luyao Yu,Xiaoqian Chen,Yingxia Guo,Jiansong You,Meiyun Shi,Yalin Xi,Lei Yin
An ultra-high performance liquid chromatography tandem mass spectrometry cubed (UHPLC/MS 3 ) assay coupled with protein precipitation and counter-extraction for detection of tricyclic glycopeptide vancomycin in human plasma was established and validated in this study. After protein precipitation and counter-extraction with dichloromethane, chromatographic separation of vancomycin and norvancomycin were performed on a reversed phase column (XBridge Peptide BEH C 18 column, 2.1 × 100 mm I.D, 3.5 μm). The transition (parent ions → fragment ions → further fragment ions) at m / z 725.3 → 144.1 → 100.1 was used for quantification of vancomycin. The transition (parent ions → fragment ions) at m / z 718.3 → 144.2 was used for detection of norvancomycin. The linear range of the developed analytical method for quantification of vancomycin was 0.5–100 µg/mL (r = 0.9989). The range of intra- and inter-day precisions of the assay among low, medium and high concentrations is between 1.88 % and 6.33 %. The sensitivity of the analytical method was significantly improved by using MS 3 technique as monitoring mode and counter-extraction with dichloromethane followed by protein precipitation as sample processing assay. The developed UHPLC/MS 3 assay was successfully applied for clinical therapeutic drug monitoring (TDM) of vancomycin in 45 human plasma samples.
研究领域:
分析化学
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临床药理学
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药物代谢动力学
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DOI
PubMed
高影响因子
Chemical Engineering and Processing-Process Intensification
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2022
·
IF 4.264
Mengfei Tian,Xianming Meng,Naveed Ahmad,Chunying Li,Zhanyu Yuan,Zidan Luo,Yu Zhang,Chunyu Liang,Chunjian Zhao
Juglone was first preliminary purified by back extraction (BE) which also completed the sustainability of microemulsion as a solvent for extraction of juglone in J. mandshurica Maxim. branches. Then, magnetic solid phase extraction (MSPE) based on bubble column (BC) was proposed to further purify the juglone, GO/ND/Fe 3 O 4 as adsorbent, which broke through the limitation that MSPE is only suitable for trace enrichment. Thus, a BE combined with MSPE based on BC (BE-MSPE/BC) technology to separate and purify juglone from J. mandshurica Maxim. was developed. Under the optimal conditions (optimal adsorption conditions: 0.5 h of adsorption time, 30 mg/mL of solid-liquid ratio, 5.5 of pH, 60 L/min of air flow; optimal desorption conditions: 0.4 h of desorption time, methanol of desorption agent, 100% of desorbent volume fraction, 8 of pH, 40 mg/mL of solid-liquid ratio, 80 L/min of air flow rate), the recovery yield and purity of juglone obtained by BE-MSPE/BC were 80.57% and 72.38%, respectively. BE-MSPE/BC, as a green separation and purification technology that can easily achieve large-scale and sustainable production, is expected to be an alternative method to separate and purify active components in biomass materials and products.
研究领域:
植物化学
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分析化学
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环境科学
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DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
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2017
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IF 3.752
Youan Ji,Yucui Hou,Shuhang Ren,Congfei Yao,Weize Wu
Forming deep eutectic solvents (DESs) by quaternary ammonium salts (QASs) and phenolic compounds to extract phenolic compounds from oil mixtures is effective. However, a small amount of neutral oil is entrained in the DESs. In this work, we proposed an anti-extraction method to remove the neutral oil entrained in DESs. Low-carbon alkanes (LCAs, including n -hexane, cyclohexane , and n -nonane) were added to model DESs to remove neutral oil. We found that the LCAs could effectively remove neutral oil entrained in the DESs. Of the three LCAs, n -hexane showed the best performance, and the removal rate of neutral oil could reach as high as 92.2%. The separation process could complete within 15 min, and the removal rate of neutral oil did not change significantly with temperature. In addition, n -hexane could effectively remove neutral oils in DESs formed by real coal tar and choline chloride. The neutral oil content in the phenolic compound product decreased to only 1.9 wt%, which greatly improves the purity of the phenolic compound product.
研究领域:
化学工程
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环境科学
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DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2018
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IF 1.712
Huifang Li,Lijuan Li,Xiaowu Peng,Lianmin Ji,Wu Li
The organic synergists, including Octanol, ethyl acetate (EA), butyl acetate (BA), methyl isobutyl ketone (MIBK), diisobutyl ketone (DIBK), N , N -bis(2-ethylhexyl) acetamide (N523) and 8-hydroxylquiolate, were added to the TBP–FeCl 3 extraction system to extract lithium from brine. The effects of concentration of organic synergist and total organic extractant, molar ratio of Fe/Li, phase ratio, counter-current extraction and the acidity of stripping agent on lithium extraction were investigated to optimize the extraction conditions. Under the optimize conditions, the results of counter-current extraction showed the mixed extraction system was the preponderance on the lithium extraction. Especially the separation of lithium in organic phase and aqueous phase and the separation mass ratio of Mg/Li increased greatly. An extraction mechanism was proposed based on the analysis of FT-IR spectra and Raman spectra.
研究领域:
化学工程
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材料科学
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DOI
高影响因子
Separations
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2025
·Vol. 12
·期 11
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IF 2.7
Zhaojin Zhang,Subhan Mahmood,Yu Cao,Shun Yao
At present, ionic liquids (ILs) are increasingly being used to extract natural products as green solvents, but their residues can lead to risks in terms of further use for the extracted herbal materials. Therefore, it is necessary to remove them with simple and effective methods. For example, after the toxic anthraquinones inPolygonum multiflorumare removed by extraction with the IL of [C4Bim][PTSA], it needs to be recovered and reused, and the useful stilbene glycosides should not suffer from obvious loss as they are the main functional components. In this study, an ultrasonic method withn-propanol was used to remove the residual [C4Bim][PTSA] in the solid powders ofPolygonum multiflorumthat had been extracted for anthraquinones. After single-factor optimization, the removal conditions were as follows: the removal temperature was 303.15 K, the solid–liquid ratio was 1:200 (w (1 g):v (200 mL)), the ultrasonic time was 40 min, and there were four operations. Under these conditions, ILs could be completely removed with almost no loss of stilbene glycosides in solid powders. After that, the IL in the extracting solution and scrubbing solution was recovered by the back-extraction method, and an IL with high purity could be obtained for reuse. The total recovery efficiency of the IL reached more than 98%. Then gas chromatography (GC) was conducted for the determination of residual ethanol andn-propanol in the solid powders ofPolygonum multiflorum, which could be used to quickly detect the contents of two organic solvents within three minutes. Besides that, the method could also be applied to the determination of residual organic solvents in the raw materials ofPolygonum multiflorum, and the results showed that the residue of ethanol andn-propanol in the solid powders were in accordance with the general provisions of the currentChinese Pharmacopoeia. According to the developed procedures and optimized conditions, the recovered IL could be reused in five runs at least. General applicability and greenness assessment for the developed process also proved that it is an ideal method, which has potential in large-scale application.
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DOI
高影响因子
ACS Omega
·
2023
·
IF 4.1
Daniu Cai,Guangcai Zhang,Dongliang Hu,Jianyang Li,Minggong Wang,Yingwu Zhang,Junsheng Yuan
For the separation of metal ions, ionic liquid-based aqueous biphasic systems (IL-ABSs) offer a promising alternative to solvent extraction. However, the incorporation of an extensive quantity of inorganic salts restricts their practical application. Because heavy metal wastewater often contains high concentrations of inorganic salts, it offers good prospects for the application of IL-ABSs in the separation of heavy metals. In this work, an IL-ABS was formed by tributyltetradecylphosphonium chloride ([P44414]Cl), and simulated high-salinity wastewater (NaCl and Na2SO4 as the main inorganic salts) was used for the separation of heavy metals. The phase diagram results indicated that the formation of a two-phase system required a relatively high salt concentration. The extraction process followed the mechanism of anion exchange; thus, heavy metals such as zinc and cadmium that formed complexes with chloride ions could be effectively extracted (extraction rate >99.5%) with a very fast rate (extraction time <1 min) at a wide pH range (pH = 2–7). After extraction, the metals could be stripped well (stripping rate >99.5%) after contact with the NaOH solution. This research provided a new approach for treating heavy metals in high-salinity effluents, which has the advantages of IL-ABS and avoids the disadvantages of adding large amounts of inorganic salts at the same time.
研究领域:
环境化学
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废水处理
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分离技术
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2011
·Vol. 173
·期 1
·267-273
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IF 2.578
Wang Yang,Luo Xiaoyu,Tang Jie,Hu Xiaoya
A method is presented for matrix separation, preconcentration and determination by hydride generation atomic fluorescence spectrometry of trace amounts of Se(IV). It is based on solidified floating drops of 1-undecanol that are capable of extracting the target analyte after chelation with a water soluble ligand and subsequent ultrasound-assisted back-extraction into a aqueous solution. Hydride generation was then accomplished by reaction with a solution of sodium borohydride. Under optimized conditions, an enrichment factor of 15 and a linear calibration plot in the range from 0.01 to 5.0 μg L −1 were achieved using a 10.0 mL sample. The detection limit (3σ) is 7.0 ng L −1 , and the relative standard deviation (RSD) is 2.1% at 1.0 μg L −1 ( n = 11). The method was applied to determination of Se(IV) in different real water samples through recovery experiments and subsequently validated against two certified reference materials.
A solidified floating organic drop microextraction coupled with hydride generation atomic fluorescence spectrometry for the determination of Se(IV) is described.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2015
·Vol. 9
·期 3
·699-705
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IF 1.956
Yue Mei-E,Li Qian,Xu Jie,Jiang Ting-Fu
A rapid, simple, and sensitive method has been developed for the analysis of triazine herbicides in honey samples by using salt de-emulsification dispersive liquid-liquid microextraction and back-extraction (SD-DLLME-BE) coupled with sweeping in micellar electrokinetic chromatography (MEKC). In the newly developed method, chloroform can be generated by the reaction of chloral hydrate and sodium hydroxide, and the chloroform droplets dispersed into bulk aqueous solution can be swiftly carried to the bottom of the centrifuge tube with the addition of calcium chloride. Then, back-extraction was utilized to extract the analytes into an aqueous solution prior to capillary electrophoresis (CE) analysis. The advantages of the new in situ DLLME lie in dispersive solvent-free and no centrifugation operation compared with the conventional DLLME. Several important parameters influencing the preconcentration and extraction efficiency of SD-DLLME-BE such as sodium dodecyl sulfate (SDS) concentration, pH, chloral hydrate and sodium hydroxide content, extraction time, and calcium chloride content were optimized. Under the optimized conditions, the enrichment factors were achieved in the range from 459 to 636. The proposed method provided a good linearity, low limits of detection (8.56 ng/mL), and good repeatability of the extractions (relative standard deviations (RSDs) below 8.8 %, n = 6). Honey samples were analyzed by the proposed method, and obtained results indicated that the proposed method provides acceptable recoveries and precisions.
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
JOM
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2018
·Vol. 70
·期 12
·2837-2845
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IF 2.145
Zhang Weiguang,Zhang Ting-An,Lv Guozhi,Zhou Weihua,Cao Xuejiao,Zhu Hangyu
The solvent extraction and separation process of Sc(III) and Fe(III) from a strongly acidic and highly concentrated ferric solution using mixtures of di(2-ethylhexyl) phosphate (D2EHPA) and tri-n-butyl phosphate (TBP) in sulfonated kerosene was studied. The effects of various parameters, including D2EHPA concentration, dosage of TBP, and phase ratio, were investigated for the extraction process. The results indicated that the extraction rate of Sc(III) was 99.72% with 1.09% Fe(III) co-extracted after two stages of counter-current extraction under optimal conditions. Moreover, saturation capacity and slope analysis were used to determine the reaction mechanism. Sc(III) is extracted in the form of HSc(SO 4 ) 2 ·4HL. Further separation of Sc(III) and Fe(III) was realized in a scrubbing and stripping process. First, 98.67% of the co-extracted iron in the loaded organic phase was scrubbed with a dilute HCl solution by a three-stage counter-current scrubbing. Then, 85.00% of Sc(III) can be stripped efficiently with a 2-mol/L NaOH solution saturated in 1-mol/L NaCl by three-stage cross-current stripping.
研究领域:
化学工程
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分析化学
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环境科学
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产品关联
DOI
高影响因子
International Journal of Minerals Metallurgy and Materials
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2023
·Vol. 30
·期 5
·877-885
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IF 4.8
Wang Junlian,Liu Lu,Xu Wen,Liu Hui,Xu Guodong,Huang Kun,Yu Fengshan,Huang Guoyong
Platinum group metals (PGMs), especially Pd, Pt, and Rh, have drawn great attention due to their unique features. Direct separation of Pd and Pt from highly acidic automobile catalyst leach liquors is disturbed by various factors. This work investigates the effect of various parameters including the acidity, extractant concentration, phase ratio A/O, and diluents on the Pd and Pt extraction and their stripping behaviors. The results show that the Pd and Pt are successfully separated from simulated leach liquor of spent automobile catalysts with monothio-Cyanex 272 and trioctylamine (TOA). Monothio-Cyanex 272 shows strong extractability and specific selectivity for Pd, and only one single stage is needed to recover more than 99.9% of Pd, leaving behind all the Pt, Rh, and base metals of Fe, Mg, Ce, Ni, Cu, and Co in the raffinate. The loaded Pd is efficiently stripped by acidic thiourea solutions. TOA shows strong extractability for Pt and Fe at acidity of 6 mol·L −1 HCl. More than 99.9% of Pt and all of the Fe are extracted into the organic phase after two stages of countercurrent extraction. Diluted HCl easily scrubs the loaded base metals (Fe, Cu, and Co). The loaded Pt is efficiently stripped by 1.0 mol·L −1 thiourea and 0.05–0.1 mol·L −1 NaOH solutions. Monothio-Cyanex 272 and TOA can realize the separation of Pd and Pt from highly acidic leach liquor of spent automobile catalysts.
研究领域:
冶金学
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化学工程
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环境科学
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DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·560-565
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IF 1.938
Yun-Kai Lv,Wei Zhang,Meng-Meng Guo,Fang-Fang Zhao,Xiao-Xue Du
A home-made centrifugal microextraction tube (CMET) was developed and applied to cloud point extraction coupled with microwave-assisted back extraction and gas chromatographic separation of six phthalate esters (PAEs) from mineral water. All phthalate esters were entrapped in the micelles of the non-ionic surfactant Triton X-114 and removed from the bulk phase by centrifugation. The obtained surfactant-rich phase was treated with water-immiscible solvents, and the target analytes were back extracted by short-term microwave application and determined by GC-FID directly. The whole process was finished in the CMET. The proposed method demonstrated good performance concerning linearity (R2 = 0.9977-0.9998), precision (2.3–5.7%), the limits of detection and quantitation (LOD, 11.5-19.3 μg L−1; LOQ, 37.0-63.3 μg L−1), spiked recoveries (89.1–96.3%) and enrichment factor (71-85). The proposed method was successfully applied for the determination of trace amount of phthalate esters in mineral water. DBP and BBP were found in actual mineral water samples.
研究领域:
分析化学
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环境毒理学
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食品安全
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分子监测
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样品前处理
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2020
·
IF 5.774
Qiyu Meng,Jiemiao Yu,Liangrong Yang,Yanqing Li,Mo Xian,Huizhou Liu
Effective isolation of 1,2,4-butanetriol (BT) from the fermentation broth with low BT concentration was achieved based on anionic reactive extraction with a combined extractant consisted of boronic acid carrier and trioctylmetylammonium Aliquat® 336 (A336) dissolved in the organic phase. The influence of various diluents, pH values, the ratio of phenylboronic acid derivatives (PBAD) to BT (α PBAD/BT ), and A336 to BT (β A336/BT ) on extraction property was investigated in detail. For model BT solutions, satisfactory extraction efficiency of 92% was obtained under the conditions of α PBAD/BT = 1.5, β A336/BT = 2, with dichloromethane as a diluent. Aqueous hydrochloric acid solutions were employed to strip BT from the organic phase. For actual fermentation broth, the extraction and stripping efficiency under the optimum conditions were 74% and 69%, respectively. Besides, compared with the original fermentation broth, 1,2,4-butanetriol has been concentrated by 4.6 times with the reactive extraction coupled hydrolysis.
研究领域:
化学工程
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生物技术
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有机化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）