JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2024
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IF 2.8
Huijie Zheng,Hansheng Liu,Xueqi Shi,Shuwen Liu,Weiyi Su,Hao Li
BACKGROUND Cadaverine is an important diamine to synthesize nylon. However, the lower product concentration in the cadaverine production makes the downstream separation more complex and costly, and has become a bottleneck for large-scale production. Therefore, in this study, the salting-out was used to efficiently separate cadaverine in the simulated fermentation broth. RESULTS After analyzing the pH of readily soluble potassium salts, three alkaline salting-out agents—K 3 PO 4 , K 2 CO 3 and K 4 P 2 O 7 —were utilized to perform the salting-out separation of cadaverine. The effects of the type and concentration of salting-out agents and cadaverine concentration on the distribution and selection behavior of cadaverine in the simulated fermentation broth were investigated. Particularly, when the concentration of cadaverine was 10 wt% and the K 3 PO 4 concentration was 500 g/kg, the recovery rate of cadaverine in the simulated fermentation broth with K 3 PO 4 salting-out agent was as high as 99.6%, which was significantly higher than that of other salting-out agents, and only 0.01 wt% cadaverine remained in the aqueous phase. CONCLUSION The salting-out ability of the three inorganic salts to cadaverine in the simulated fermentation broth was K 3 PO 4 >K 2 CO 3 >K 4 P 2 O 7 . Furthermore, within a certain range of cadaverine concentrations, the content of cadaverine in the aqueous phase and the amount of water residue in the organic phase could be calculated using a linear regression equation. Since inorganic salts have the advantages of good stability, low cost, non-toxicity and harmlessness, the salting-out process established in this study shows good potential for industrial application. © 2024 Society of Chemical Industry (SCI).
研究领域:
生物化学
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化工工程
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产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2020
·
IF 3.644
Xiao Yiwen,Zhang Zhibin,Wang Ya,Gao Boliang,Chang Jun,Zhu Du
Succinic acid is an important chemical and raw material widely used in medicine, food, biodegradable materials, fine chemicals, and other industrial fields. However, traditional methods for purifying succinic acid from fermentation broth are costly, poorly efficient, and harmful to the environment. In this study, an efficient method for purifying succinic acid from the fermentation broth of Escherichia coli NZN111 was developed through crystallization and co-crystallization with urea. First, the filtrate was collected by filtering the fermentation broth, and pH was adjusted to 2.0 by supplementing sulfuric acid. Crystallization was carried out at 8°C for 4 h to obtain succinic acid crystals. The recovery rate and purity of succinic acid were 73.4% and over 99%, respectively. Then, urea was added to the remaining solution with a mass ratio of urea to residual succinic acid of 4:1 (murea/mSA). The second crystallization was carried out at pH 2 and 4°C for 12 h to obtain succinic acid-urea co-crystal. The recovery rate of succinic acid residue was 92.0%. The succinic acid-urea crystal was further mixed with phosphorous acid (4.2% of the mass of succinic acid co-crystal) and maintained at 195°C for 6 h to synthesize succinimide, and the yield was >80%. This novel and efficient purification process was characterized by the significantly reduced urea consumption, and high succinic acid recovery (totally 95%), and high succinimide synthesis yield (80%). Thus, this study potentially provided a novel and efficient strategy for the industrial production of succinic acid and succinimide.
研究领域:
生物工程
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化工
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Shengchao Xu,Wanting Ma,Junxiong Zhao,Jiaming Yu,Qifeng Wei,Xiulian Ren
Lactic acid (LA) is a typical high added-value chemical and platform molecule derived from renewable biomass. To realize high efficiency and green separation of LA is of both scientific and practical significance. In this work, we proposed a novel mechanism of in-situ formation of hydrophobic deep eutectic solvents (HDES) to couple the separation of LA and synthesis of HDES . Due to the high solubility of LA as hydrogen bond donor in water, three hydrophobic lactams ( N -Octyl-2-pyrrolidone (NOP), N -Dodecyl-2-pyrrolidone (NDP) and 2-Octyl-4-isothiazolin-3-one (OIT)) were optioned as hydrogen bond acceptors. The effect of lactam molecular structure on separation efficiency was discussed and compared in detail. The compositions of HDES forming in-situ were found to be 1.50 for NOP and NDP to LA while 1.20 for OIT to LA using experiments of slope method and continuous variations method. The key evidence of formation of HDES was confirmed by FT-IR, frontier molecular orbital analysis and interaction region indicator analysis. The multistage counter-current extraction experiment results showed the in-situ formation of HDESs can transfer 99% of LA from fermentation broth to the HDES, and LA was separated completely from the impurities. This work provided a new perspective on lactic acid separation and its potential industrial applications.
研究领域:
生物化学
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绿色化学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2020
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IF 5.774
Yan Li,Jian-Ying Dai,Zhi-Long Xiu
The salting-out extraction (SOE) systems based on ionic liquids (ILs) have attracted extensive attention in the separation of bio-based products, in which imidazolium ILs were widely studied. However, the high cost and toxicity have hindered their further industrial application. Hydroxylammonium ILs have the characteristics of cheap raw material, simple synthesis process and low toxicity, but are rarely used in the SOE systems. In this work, five hydroxylammonium ILs (2 cations and 4 carboxylate anions) were synthesized and used in SOE of bio-chemicals. The phase forming abilities of ILs with K 3 PO 4 and H 2 O were affected by the hydrophilicity of anions and cations. With the increase of carbon chain length, phase forming abilities of ILs increased, while the temperature had little influence. The partition behaviors of acetoin, ILs and 2,3-butandiol, and the selectivity of acetoin to organic acids were investigated and compared at different concentrations of ethanolammonium butyrate (EOAB) and K 3 PO 4 . In a SOE system consisted of 6% EOAB-38% K 3 PO 4 (w/w), the recovery of acetoin, IL and 2,3-butandiol was 92.7%, 76.0% and 86.0%, respectively, and the selectivity of acetoin to lactic acid and acetic acid was 16.46 and 3.85, respectively. The ATR-IR spectra showed the hydrogen bonds formed between acetoin and O-H, N-H, –COO – of hydroxylammonium IL played an important role in the efficient extraction of acetoin from fermentation broths.
研究领域:
生物化工
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
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2014
·Vol. 19
·期 7
·9675-9688
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IF 2.095
Pu Jing,Li-Hua Song,Shan-Qi Shen,Shu-Juan Zhao,Jie Pang,Bing-Jun Qian
Red radish (RaphanusL.) pickles are popular appetizers or spices in Asian-style cuisine. However, tons of radish brines are generated as wastes from industrial radish pickle production. In this study, we evaluated the dynamic changes in colour properties, phenolics, anthocyanin profiles, phenolic acid composition, flavonoids, and antioxidant properties in radish brines during lactic acid fermentation. The results showed that five flavonoids detected were four anthocyanins and one kaempferol derivative, including pelargonidin-3-digluoside-5-glucoside derivatives acylated withp-coumaric acid, ferulic acid,p-coumaric and manolic acids, or ferulic and malonic acids. Amounts ranged from 15.5–19.3 µg/mL in total monomeric anthocyanins, and kaempferol-3,7-diglycoside (15–30 µg/mL). 4-Hydroxy-benzoic, gentisic, vanillic, syringic,p-coumaric, ferulic, sinapic and salicylic acids were detected in amounts that varied from 70.2–92.2 µg/mL, whereas the total phenolic content was 206–220 µg/mL. The change in colour of the brine was associated with the accumulation of lactic acid and anthocyanins. The ORAC and Fe2+chelation capacity of radish brines generally decreased, whereas the reducing power measured as FRAP values was increased during the fermentation from day 5 to day 14. This study provided information on the phytochemicals and the antioxidative activities of red radish fermentation waste that might lead to further utilization as nutraceuticals or natural colorants.Keywords:radish;brine;fermentation;phytochemicals;antioxidant
研究领域:
食品科学
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植物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Hao Li,Haoyang Wang,Osama M. Darwesh,Jingjing Du,Shan Liu,Chunli Li,Jing Fang
Adsorption is considered to be a promising butanol recovery method for solving the issue of inhibition in the ABE (acetone-butanol-ethanol) fermentation. As a byproduct in the second generation biobutanol industry, lignin was found to be a good adsorbent for the butanol enrichment. It is conducive to the full utilization of renewable lignocellulose biomass resource. Kinetic and equilibrium experiments indicated that lignin had a satisfactory adsorption rate and capacity that are comparable to those of many synthetic materials. Multicomponent adsorption experiments revealed that lignin had higher adsorption selectivity toward butanol than that of ethanol and acetone. The adsorption capacity of lignin for butanol first increased and then gradually decreased with increasing temperature. And maximum adsorption capacity reached 304.66 mg g −1 at 313 K. The inflection point of temperature is close to the ABE fermentation temperature of 310 K. The condensed butanol by desorption was 145 g L −1 , with a satisfying regeneration performance. 1 H NMR and FT-IR spectra indicated that the aromatic units of lignin formed π -systems with A/B/E. The π -system is particularly significant for butanol due to its longer hydrocarbon chain. These results could contribute to the emerging lignin-based materials for butanol separation.
研究领域:
生物工程
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环境科学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2018
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IF 3.927
Zhen Li,Ling Yan,Jinjie Zhou,Xiaoli Wang,Yaqin Sun,Zhi-Long Xiu
The separation of 1,3-propanediol (1,3-PD), butyric acid (BA) and acetic acid (HAc) from the fermentation broth was studied by two-step salting-out extraction. In the first salting-out extraction, the partition coefficient and recovery of BA reached 42.21 and 96.42%, respectively, under the optimal condition of 25 wt% NaH 2 PO 4 /30 wt% n-butyl acetate. Subsequently, 91.28% of BA in the organic phase could be recovered through back-extraction when sodium carbonate solution was mixed with organic phase with initial phase ratio of n-butyl acetate solution to alkaline solution being 2:1, in which the molar ratio of sodium carbonate to organic acids was 3:5. Finally, 50% (v/v) ethanol (95%) was added to the bottom phase for the second step salting-out extraction. And the partition coefficient and recovery of 1,3-PD were 9.40 and 95.50%, and those of HAc were 7.46 and 94.40%, respectively. All the cells and most of the proteins (97.16%) could be removed. Effective separation of 1,3-PD from BA was realized by two-step salting-out extraction, which provides a potential method for separation of 1,3-PD, BA and HAc on an industrial scale.
研究领域:
生物工程
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化学工程
✓ 已验证
DOI
高影响因子
PROCESS BIOCHEMISTRY
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2021
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IF 3.757
Lining Zheng,Cheng Zhang,Xian Wu,Ling Liu,Hao Zhang
Tomato gray mold, caused by Botrytis cinerea , is one of the most devastating diseases affecting tomato plants. In this study, Pantoea jilinensis D25 fermentation broth (PJFB) was used to inhibit B. cinerea growth. The optimum fermentation time was 7 days. PJFB antifungal activity decreased when PJFB was heated to ≥60°C, whereas it was the strongest at pH 8. Moreover, ultraviolet treatment for a long time reduced its antifungal effect. PJFB, applied at 10 % (v/v), could inhibit B. cinerea growth by inhibiting its mycelial diameter, conidial production, and mycelial dry weight with the inhibition rates of 57.3 %, 51.2 %, and 78.6 %, respectively. PJFB could also increase conductivity and malondialdehyde (MDA) content in B. cinerea . Furthermore, PJFB inhibited B. cinerea on not only the leaf tissues but also the seedlings of tomato. The secondary metabolites in PJFB were determined through gas chromatography–mass spectrometry. Through laboratory toxicity analysis, we found that the major compounds in PJFB were 1,2-benzenedicarboxylic acid bis(2-methylpropyl) ester (14.3098 %), 2,4-di- tert -butylphenol (3.8036 %), 2,6,10,14-tetramethyl pentadecane (2.9947 %), and 1,3-dimethyl naphthalene (1.8586 %).
研究领域:
植物病理学
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微生物学
✓ 已验证
DOI
高影响因子
Biotechnology Journal
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2024
·Vol. 19
·期 6
·2400040
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IF 3.2
Shibo Jiang,Ying Lin,Suiping Zheng
IMP (inosinic acid) is a crucial intermediate in the purine metabolic pathway and is continuously synthesized in all cells. Besides its role as a precursor for DNA and RNA, IMP also plays a critical or essential role in cell growth, energy storage, conversion, and metabolism. In our study, we utilized the circularly permuted fluorescent protein (cpFP) and IMP dehydrogenase to screen and develop the IMP biosensor, IMPCP1. By introducing a mutation in the catalytically active site of IMPCP1, from Cys to Ala, we disrupted its ability to catalyze IMP while retaining its capability to bind to IMP without affecting the IMP concentration in the sample. To immobilize IMPCP1, we employed the SpyCatcher/SpyTag system and securely attached it to Magarose-Epoxy, resulting in the development of the IMP rapid test kit, referred to as IMPTK. The biosensor integrated into IMPTK offers enhanced stability, resistance to degradation activity, and specific recognition of IMP. It is also resistant to peroxides and temperature changes. IMPTK serves as a rapid and stable assay for analyzing IMP concentrations in fermentation broth. Within the linear range of IMP concentrations, it can be utilized as a substitute for HPLC. The IMPTK biosensor provides a reliable and efficient alternative for monitoring IMP levels, offering advantages such as speed, stability, and resistance to environmental factors.
研究领域:
生物化学
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生物技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2019
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IF 7.015
Zhihao Si,Di Cai,Shufeng Li,Changwei Zhang,Peiyong Qin,Tianwei Tan
Recently, zeolitic imidazolate framework-8 (ZIF-8) has been recognized as one of the most potential doping particles for pervaporation separation of organics because of its unique pore structure and high surface area. However, in this work, ZIF-8-based mixed matrix membrane (MMM) was found to have poor stability in separating acetone-butanol-ethanol (ABE) fermentation broth due to the organic acids caused the degradation of ZIF-8 framework. To improve the stability of ZIF-8-based MMM in practical application, ZIF-8-derived nanoporous carbon (ZNC) was prepared via direct carbonization and used to replace ZIF-8 in MMM. Results indicated the acid-stability, specific surface area and pore volume of ZNC were all remarkably improved compared with those of ZIF-8. The ZNC-based MMM showed high hydrophobicity and good compatibility between particles and polymer. More attractively, during 100 h of continuous operation for ABE recovery from fermentation broth, ZNC incorporated MMM exhibited a stable performance with an average total flux of 1870 g m −2 h −1 and a n -butanol separation factor of 20.
研究领域:
化学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2024
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IF 5.1
Aobin Li,Maosong Wang,Huimin Sang,Wei Ke,Minghui Qiu,Qi Zhang,Xianfu Chen,Yiqun Fan
Ceramic membranes are increasingly recognized for their effectiveness in processing fermentation broths. However, developing ceramic membranes with superior anti-fouling properties remains a challenge for large-scale applications. Iron oxide nanomaterials have gained significant research interest as a cost-effective and readily available solution for mitigating membrane fouling , thanks to their excellent hydrophilicity. This study introduces ceramic membranes enhanced with iron oxide via a facile sol-gel technique. The membrane's resistance to fouling, hydrophilicity, adhesion force, and surface potential were assessed. Post-modification, a consistent distribution of iron elements on the membrane surface was achieved, with negligible alteration in pore size. Although the pure water permeance (450 ± 45 L m −2 h −1 ·bar −1 ) did not change significantly, the permeance of the modified membrane (225 L m −2 h −1 ·bar −1 ) was nearly 20 % higher than that of the original membrane when separating a 1000 ppm oil-in-water emulsion, with both membranes achieving 99 % oil droplet rejection. Moreover, the flux recovery rate of the modified membrane surpassed that of the original membrane by approximately 30 %, indicating a significant improvement in anti-fouling performance. Upscaling experiments further validated the modified membrane's anti-fouling performance and capability to rapidly process broths with high oil content. Overall, this study presents a novel anti-fouling ceramic membrane for the treatment of oil-containing fermentation systems.
研究领域:
生物工程
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材料科学
✓ 已验证
DOI
高影响因子
FUEL
·
2019
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IF 5.128
Shaoqu Xie,Chuhan Fu,Wenli Song,Yulei Zhang,Conghua Yi
Developing a simple and efficient process for the conversion of biomass to petrol, diesel and jet fuels are our urgent mission due to the limitation of fossil fuels. Biobutanol is an important biomass-derived biofuel that can be converted to longer-chain petrol-range, diesel-range and jet-range fuels. Basing on the obtained concentrated acetone-1-butanol-ethanol (ABE) fermentation broth through a prefractionator, we report a palladium-catalysed alkylation of acetone with ethanol and 1-butanol to more efficiently produce C 5 –C 11 fuel precursors in a reactor, where all the precursors and reactants were separated simultaneously by the salting-out effect of K 3 PO 4 . The precursors include ketones and a small amount of alcohols that can be reduced to alkanes for use as liquid transportation fuels. Tuning the reaction conditions can obtain different proportions of C 5 –C 7 products (monoalkylation) and C 9 –C 11 products (double alkylation) and the overall product contents accounted for up to 82 wt%. More than 99.5% of water from the concentrated ABE fermentation broth was removed by the salting-out effect of K 3 PO 4.
研究领域:
生物化学
·
能源科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）