PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Zepeng Yang,Huangling Wang,Chen Huang,Cui Wang,Zimeng Geng,Fei Lyu
Asian longhorned beetle ( Anoplophora glabripennis ) has caused significant economic and ecological damage. However, environmentally friendly control strategies remain limited. Elucidating the adult's feeding preference is critical for developing effective and sustainable pest management approaches. Therefore, we firstly compared the feeding preference of adults on the Salix babylonica branch with diameters of 0.5-cm, 2-cm, 4-cm, 8-cm, 16-cm. The results showed that both female and male significantly preferred 2-cm branches over the others. Subsequently, we analyzed metabolites allocation patterns across these branch types, identifying a total of 2216 metabolites. Further analyses showed that 1500 differentially accumulated metabolites (DAMs) in branches with diameters of 0.5-cm, 4-cm, 8-cm, 16-cm compared to 2-cm samples. These DAMs were significantly enriched in flavonoid, flavone and flavonol biosynthesis pathways when comparing the 2-cm branches to others. The contents of 10 DAMs in flavonoid, flavone and flavonol biosynthesis pathways showed a significant negative correlation with feeding consumptions. To evaluate the antifeeding effects of flavonoid-related metabolites, four economically viable and practically flavonoid-related metabolites (+)-taxifolin, naringenin, kaempferol, and phlorizin were selected for feeding bioassays. They significantly inhibited feeding consumption, with (+)-taxifolin, naringenin, and phlorizin reducing by up to 70 %. Dose-response experiments confirmed varying concentrations of these compounds significantly suppressed feeding consumption. Our findings demonstrate that the allocation patterns of flavonoid compounds in branches of different diameters regulate the feeding preferences of A. glabripennis . This study explores a metabolomics-driven approach that centers on size-structured flavonoid reprogramming in S. babylonica branches, aiming to establish a sustainable strategy for disrupting A. glabripennis 's feeding preference.
研究领域:
昆虫学
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植物生理学
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化学生态学
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次生代谢
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林业害虫管理
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分子生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2015
·
IF 2.837
Kai Yang,Shan-Shan Guo,Zhu-Feng Geng,Chun-Xue You,Wen-Juan Zhang,Yin-Ping Li,Cheng-Fang Wang,Shu-Shan Du,Zhi-Wei Deng
Five new sulphur-containing amides ( 2 , 5 – 8 ) and three known analogue compounds ( 1 , 3 – 4 ) were isolated from the leaves of Glycosmis lucida which were collected in Yunnan province, China. Their structures were elucidated with NMR spectroscopy and mass spectrometry. The compounds were identified as N -( p -hydroxyphenethyl)-3-(methylsulfonyl)-propenamide ( 1 ), N -[2-(4- hydroxyphenyl)-ethyl]-3-methanesulfonyl- N -methyl-propionamide ( 2 ), methylgerambullin ( 3 ), methylisogerambullone ( 4 ), methylgerambullindiol ( 5 ), N -{2-[4-(6,7-hydroxy-3,7-dimethyl-oct- 2-enyloxy)-phenyl]-ethyl}-3-methanesulfonyl- N -methyl-acrylamide ( 6 ), N -{2-[4-(6,7-dihydroxy- 3,7-dimethyl-oct-2-enyloxy)-phenyl]-ethyl}-3-methanesulfonyl- N -methyl-propionamide ( 7 ), ( E )- N -{2-[4-(6-hydroxy-7-dimethyl-oct-2-enyloxy)-phenyl]-ethyl}-3-methanesulfinyl- N -methyl- acrylamide ( 8 ). Feeding deterrent activities of these surphur-containing derivatives against Tribolium castaneum were assayed for the first time. Compound 2 , 3 , 6 possessed significant feeding deterrent activities against T. castaneum adults (EC 50 = 311.6, 244.9 and 108.3 ppm, respectively). These results demonstrated that some sulphur-containing amides are promising candidates as insect antifeedant agents.
研究领域:
植物化学
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昆虫学
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天然产物研究
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PEST SCIENCE
·
2024
·
IF 4.3
Liu Yu-Xiao,Han Wen-Hao,Wang Jun-Xia,Zhang Feng-Bin,Ji Shun-Xia,Zhong Yu-Wei,Liu Shu-Sheng,Wang Xiao-Wei
Plants face an array of insect herbivores and have evolved complex defense approaches against various insect feeding strategies. However, little is known about how plants respond to successive attacks by herbivores with different feeding modes and coordinate their diverse defense mechanisms. In this study, we unveil that inducible jasmonic acid (JA) accumulation in response to leaf-chewing insects augments plant resistance and repellence to sequential leaf-chewing insect (caterpillar) and phloem-feeding insect (whitefly) infestations. Conversely, constitutive and whitefly inducible salicylic acid (SA) accumulation exclusively bolsters defense against later-stage whitefly invasion. Through assessments of herbivore performance and preferences on wild-type, JA-deficient, and SA-deficient plants, we show that JA/SA levels regulate plant resistance to both initial and sequential herbivores. Notably, JA or SA accumulation due to caterpillar or whitefly attacks does not substantially affect constitutive levels of the other compound, despite their antagonistic crosstalk. Furthermore, exogenous JA application in tobacco elicits efficient defense against successive caterpillar and whitefly assaults, surpassing SA's efficacy, albeit with associated growth penalties. Our discoveries demonstrate that plants can tailor their defense strategies against initial and sequential insects with different feeding modes. This customized defense is facilitated by JA/SA responses and their intricate cross-talk while taking account of the growth-defense trade-off.
研究领域:
植物学
·
生态学
✓ 已验证
产品关联
DOI
高影响因子
STAR Protocols
·
2021
·
IF 0
Yunjing Wang,Qian Gong,Fan Huang,Linfang He,Yule Liu
Summary Wounding evokes transient increases in cytosolic calcium (Ca 2+ ) concentration. Visualizing real-time Ca 2+ flux provides new insights into Ca 2+ -signaling pathways. Here, we outline a protocol to detect insect feeding-induced Ca 2+ flux elevation in Nicotiana benthamiana leaves based on the GCaMP3 reporter system by Leica fluorescence stereo microscopes (LFSM). LFSM combines super-fast manual screening with high-end imaging capabilities. Through this protocol, we can clearly observe the calcium flow after aphid’s piercing-sucking. Additionally, we describe a protocol to quantify Ca 2+ level using LFSM. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) .
研究领域:
植物生物学
·
分子生物学
·
昆虫学
✓ 已验证
DOI
PubMed
PLANT CELL AND ENVIRONMENT
·
2024
·
IF 6
Huan Chang,Chengli Li,Tengfei Zhu,Shouping Cai,Jie Chen,Fangfang Zhan,Liqiong Zeng,Yu Fang,Gongfu Ye,Jian Li,Jun Su
Lymantria xylina is the most important defoliator, damaging the effective coastal windbreak tree species Casuarina equisetifolia . However, the underlying genetic mechanisms through which C . equisetifolia responds to L . xylina attacks remain unknown. Here, we compared the transcriptional, phytohormone and metabolic differences between susceptible (S) and resistant (R) C . equisetifolia cultivars in response to L . xylina feeding. The main L . xylina -induced resistance in C . equisetifolia was a jasmonate (JA) response and JA synthesis was highly induced by L . xylina feeding at both the transcriptional and metabolic levels, thus promoting flavonoid accumulation. The JA response was highly activated by L . xylina feeding on the R but not in the S cultivar, although the JA signalling pathway was intact in both cultivars. We found a single amino acid mutation in the homologues of glutamate receptor-like protein 3.6 (CeGLR3.6 T807I ) in the S cultivar. Compared with the GLR3.6 homologues in the R cultivar, phosphorylation of CeGLR3.6 T807I was not induced by insect feeding, leading to a decreased JA response in the S cultivar. Collectively, this study provides new insights into the function of CeGLR3.6 in regulating the JA response of C . equisetifolia to L . xylina feeding.
研究领域:
植物科学
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分子生物学
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昆虫学
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遗传学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Yi Wang,Wei-Na Kong,Rui-Wei Shen,Hang Chen,Yu-Song Zhao,Jie Li,Rui-Yan Ma
BACKGROUND Grapholita molesta is an important pest of rosaceous fruit trees. Female oviposition affects larval infestation levels in orchards. This behavior, shaped by larval feeding experiences and female olfactory responses, points to an experience-modulated semiochemical strategy based on host plant secondary metabolites. To elucidate the combined effects of non-volatile and volatile components on female oviposition, we analyzed the volatile profiles of peach, pear, and apple shoots/fruits under intact, mechanically injured, and larval damage states throughout the growing season, along with female oviposition preferences, and compared olfactory responses (electroantennogram (EAG) and wind-tunnel experiments) and oviposition bioassays with benzaldehyde (amygdalin hydrolysate) between females with two larval amygdalin-feeding experiences. RESULTS Female oviposition preference and benzaldehyde emissions, both modulated by host factors, exhibited synchronous fluctuations. In EAG and wind-tunnel experiments, females not fed amygdalin during larval stages exhibited a strong attraction to benzaldehyde at concentrations of 10 and 0.1 μg/μL; however, females from larvae fed amygdalin showed a weaker long-distance orientation but greater responses to benzaldehyde during close-range approach and landing behaviors. Females fed amygdalin during larval stages were more likely to be responsive to lower concentrations of benzaldehyde (0.01, 0.1, and 1.0 μg/μL) for oviposition. In contrast, close-range attraction and oviposition behaviors were stronger for females that developed from amygdalin-fed larvae than those that were not fed amygdalin. CONCLUSION Dietary exposure to amygdalin during the larval stages increased the olfactory responses of females to benzaldehyde. Female oviposition behavior is co-regulated by larval experience derived from amygdalin ingestion and benzaldehyde as a host semiochemical signal. © 2025 Society of Chemical Industry.
研究领域:
昆虫行为学
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化学生态学
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农业昆虫学
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害虫综合治理
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嗅觉生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 2
·811-820
·
IF 4.462
Jing Gao,TongLai Tao,Steven P. Arthurs,Fengxian Ye,Xincheng An,Mubasher Hussain,Runqian Mao
Background Herbivores may influence each other directly and through plant mediated inter-specific interactions. The Asian citrus psyllid ( Diaphorina citri Kuwayama) and citrus aphids are key pests that can co-exist on citrus, but their plant-mediated interaction between them is unknown. Here we investigated plant-mediated effect of two citrus aphid species, the polyphagous Aphis spiraecola Patch and the oligophagous Aphis ( Toxoptera ) citricidus (Kirkaldy) on the feeding behavior and reproduction of Diaphorina citri , and explored the underlying mechanisms. Results In comparison with those on aphid free plants, Diaphorina citri had decreased reproduction and reduced phloem feeding on Aphis spiraecola pre-infested plants, while the reproduction and feeding efficiency were increased on Aphis citricidus pre-infested plants. Jasmonic acid (JA) dependent defense was significantly activated by Diaphorina citri feeding on Aphis spiraecola pre-infested plants, but was suppressed by Diaphorina citri feeding on Aphis citricidus pre-infested plants compared with that on aphid free plant. By contrast, only one tested marker gene in salicylic acid (SA) signaling was activated by Diaphorina citri feeding on Aphis spiraecola pre-infested plants. Furthermore, exogenous application of methyl jasmonate, but not SA, conferred resistance against Diaphorina citri in our citrus trials. Conclusion Our results indicate that pre-infestation by two citrus aphid species differentially altered Diaphorina citri induced citrus JA dependent defense, which resulted in different effect on subsequent Diaphorina citri performance. © 2022 Society of Chemical Industry.
研究领域:
植物科学
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昆虫学
·
植物与害虫互作
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分子植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Tengfei Liu,Jianguo Niu,Yuying Gao,Xuan Liu,Jianya Su
Flonicamid is an insecticide with a unique mode of action, primarily inhibiting the feeding behavior of sap-sucking insects. However, its molecular target remains controversial. Reports indicate that both flonicamid and pymetrozine affect insect behavior and mobility. To investigate further, we compared the susceptibility of three insect species ( Myzus persicae , Nilaparvata lugens , and Drosophila melanogaster ) to flonicamid and pymetrozine under two different feeding postures. Results showed that feeding posture did not affect the survival curves or mean lifespans of the three insects. However, insect lifespans were significantly reduced under exposure to either insecticide compared to untreated controls. The effects of the two insecticides on susceptibility and lifespan under different feeding postures were markedly different: feeding posture significantly influenced the sensitivity of aphids, planthoppers, and fruit flies to pymetrozine but had minimal effects on the sensitivity of aphids and planthoppers to flonicamid. Flonicamid had only a minor impact on fruit flies' sensitivity. Survival curve analysis also revealed significant differences under pymetrozine stress, while no such differences were observed under flonicamid stress. These findings indicate that flonicamid exerts different toxicological effects on target insects (aphids and planthoppers) versus non-target insects (fruit flies), depending on feeding posture. This suggests that the toxicological mechanisms of flonicamid differ substantially between target and non-target organisms, with implications that findings in non-target insects may not directly apply to target pests. Our study provides valuable insights and directions for further research into flonicamid's mode of action, aiding in elucidating its underlying mechanisms.
研究领域:
昆虫学
·
毒理学
·
农药科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Qing Gao,Nuo Jin,Zhifan Shen,Jingran Guo,Haiping Lu,Shanjie Han,Wenhan Xiao,Jing Lu,Yonggen Lou
Plants perceive signals associated with herbivore eggs and in response initiate ovicidal defence. However, which phytohormone pathways regulate this defence and which defensive compounds dominate it remains largely unknown. Here, we found that the hatching rate of eggs of white-backed planthopper (WBPH) Sogatella furcifera was significantly lower on a japonica rice variety P81 than an indica rice variety NB44. When infested by WBPH, P81 plants showed higher jasmonic acid (JA) and abscisic acid (ABA) responses than did NB44 plants; moreover, P81 plants produced the ovicidal compound benzyl benzoate and exhibited higher levels of some flavonoids, phenolamides, and volatiles than were found in NB44 plants. Impairing the ABA-signalling pathway, especially the JA-signalling pathway in P81 plants enhanced the survival of WBPH eggs. Decreasing levels of some flavonoids and phenolamides in P81 plants promoted WBPH egg survival. In vitro bioassays revealed that both naringenin and sakuranetin promote the ovicidal effect of benzyl benzoate on WBPH. The results demonstrate that JA- and ABA-signalling pathways jointly regulate the rice ovicidal defence against WBPH, and that benzyl benzoate, as well as some other compounds, such as naringenin and sakuranetin, contribute to the mortality of WBPH eggs.
研究领域:
植物科学
·
昆虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 1
·173-182
·
IF 4.462
Dongbiao Lü,Yanlu Dong,Zizheng Yan,Xueying Liu,Yongjun Zhang,Daibin Yang,Kanglai He,Zhenying Wang,Ping Wang,Xiangqun Yuan,Yiping Li
BACKGROUND Spodoptera frugiperda is an important invasive agricultural pest that causes huge economic losses worldwide. Gut microorganisms play a vital role in host feeding, digestion, nutrition, immunity, growth and insecticide resistance. Illumina high-throughput sequencing was used to study the gut microbial community dynamics across the life cycle (egg, 1st to 6th instar larvae, pupae, and male and female adults) of S. frugiperda fed on maize leaves. Furthermore, the gut microbial community and food intake of the 5th instar S. frugiperda larvae were studied after feeding them antibiotics. RESULTS Enterobacteriaceae and Enterococcaceae dominated the gut during growth and feeding of the larvae. The relative abundance of Enterobacteriaceae was higher in the 4th and 6th instar larvae. With the increase in larval feeding, the relative abundance of Enterococcaceae gradually increased. In addition, principal coordinate analysis and linear discriminant effect size analysis confirmed differences in the structure of gut microbiota at different developmental stages. After antibiotic treatment, the relative abundance of Firmicutes, Proteobacteria and Fusobacteriota decreased. The relative abundance of Enterococcus and Klebsiella decreased significantly. Antibiotic treatment inhibited the gut flora of S. frugiperda , which decreased larval food intake and body weight gain, and prolonged the larval stage. CONCLUSION The composition of the gut bacterial community plays an important role in the growth, development, and feeding of S. frugiperda . The results have a certain theoretical value for the development of bio-pesticides targeting intestinal flora. © 2022 Society of Chemical Industry.
研究领域:
昆虫学
·
微生物学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Insect Science
·
2025
·
IF 3
Delong Kong,Ruyue Zhai,Qiling Liang,Hui Guo,Zitong Wang,Changlu Wang,Tongen Ye,Junli Xiao,Lanfeng Wang,Lufei Xie,Tao Ma,Chunsheng Jia,Desen Wang
Desiccant dusts have been shown to be effective materials in the management of bed bugs ( Cimex spp.). Past research primarily focused on exploring the direct lethal effect of dusts against bed bugs, whereas information on their sublethal effects on bed bugs are limited. In this study, we evaluated the lethal effect of 3 dust products (Johnson's ® Baby powder, Vazor DE powder ™ , and CimeXa™; abbreviated as Baby powder, DE, and CimeXa) against the tropical bed bug, Cimex hemipterus (F.), under laboratory conditions. Results showed that the 3 dust products caused 97%–100% mortality to tropical bed bugs within 14 d, both in no-choice and choice experiments. However, in a brief exposure experiment (crossing a 2.5-cm-wide band), Baby powder exposure caused a significantly lower mortality (23%) than DE (88%) and CimeXa (100%). The more effective dusts (DE and CimeXa) were selected for further evaluation of their sublethal effects on C. hemipterus . DE and CimeXa caused significantly higher mortality (48%–58%) through horizontal transfer than that of control (6%). Brief exposure to DE and CimeXa dusts did not affect tropical bed bug activity and their response to CO 2 . However, CimeXa-exposed tropical bed bugs exhibited reduced feeding and lowered survival rate after feeding. Moreover, both DE and CimeXa dust bands showed strong barrier effects on the crawling of tropical bed bugs. Our results indicate that both DE and CimeXa have great potential for preventing C. hemipterus from reaching protected areas and for controlling C. hemipterus .
研究领域:
昆虫学
·
害虫管理
·
杀虫剂毒理学
·
应用生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）