npj Clean Water
·
2024
·Vol. 7
·期 1
·1-13
·
IF 10.4
Chen Chao,Yang Yu,Choo Kwang-Ho,Ng How Yong,Takizawa Satoshi,Hou Li-an
Membrane biofouling poses an ongoing challenge in seawater reverse osmosis (SWRO) desalination. Here we delved into the impact of seasonal variations in microbial communities on membrane biofouling and innovatively fabricated quorum sensing inhibitors (acylase (AC) and methyl anthranilate (MA))-modified membranes to combat it. Results indicated that Proteobacteria dominated in seawater and membrane biofilm across all seasons, while other phyla varied seasonally. At the class level, the two leading bacteria on the membrane were Gamma- and Alphaproteobacteria , constituting 14–48% and 4–27%, respectively. Genera like Arcobacter , Vibrio , and Rhodobacteraceae were identified as keystone species that exhibited a significant positive correlation with extracellular polymeric substance (EPS) and biofilm formation, leading to a substantive reduction in membrane flux by 70 to 77%. The introduction of AC and MA inhibitors on the membrane suppressed keystone bacteria Rhodobacteraceae and Arcobacter and affected their metabolism, thereby significantly reducing EPS by 65–69% and 55–59%, respectively, and alleviating membrane flux decline by 30–32% and 18–22%, respectively, compared to the pristine membrane. These findings shed new light on the seasonal patterns of membrane biofouling and provide valuable insights into anti-biofouling strategies based on QS inhibition for collaborative biofilm formation.
研究领域:
环境科学
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微生物学
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水处理
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Hengzhen Ye,Yanjie Zhang,Lu Wei,Hao Feng,Qiongyao Fu,Zhiqiang Guo
Background Chromium (Cr) mainly has two stable forms: Cr 3+ and Cr 6+ . Cr and its compound are widely used in the printing, dyeing, leather making, and metallurgy industry. They are evitably released into the environment and pose a significant threat to creatures, for instance, the excessive chromium (Cr) burden in the marine ecosystem is often harmful to fish. Intestinal microbiota greatly affects fish performance, but how waterborne Cr affects fish intestinal microbiota is unclear. To test the hypothesis that the waterborne Cr exposure could significantly affect fish’ intestinal microbiota homeostasis, and the effect was highly dependent on Cr concentration and speciation, the juvenile leopard coral grouper Plectropomus leopardus were exposed to waterborne Cr 3+ and Cr 6+ (0.1, 0.5 ppm) for 7 days, and the intestinal microbiota was determined by Amplicon sequencing of the 16S rRNA. Results In all Cr treatment groups, the alpha diversity of intestinal microbiota communities of P. leopardus was decreased. The phyla Proteobacteria, Firmicutes, Actinobacteria, Chloroflexi and Bacteroidetes were the dominant intestinal microbiota. The Chao index diversity significantly declined in Cr treatment group, indicating the intestinal microbiota community structure was changed. Among the dominant intestinal microbiota, Proteobacteria was most sensitive to Cr exposure, and it increased after xposure. The PICRUSt predicted that 0.5 ppm Cr 3+ expousure caused metabolism disordered in the intestinal of P. leopardus . Conclusions Waterborne Cr 3+ and Cr 6+ significantly disturbed intestinal microbiota homeostasis in P. leopardus , including their diversity, composition, and community structure. The metabolism level of intestinal microbiota in P. leopardus was decreased by Cr 3+ exposure. High concentrations of Cr 3+ may pose potential risks to the intestinal homeostasis of P. leopardus .
研究领域:
环境科学
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微生物学
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水生毒理学
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海洋生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Qingqing Cao,Yan Li,Yan Kang,Zizhang Guo
Polycyclic aromatic hydrocarbons (PAHs), as hazardous pollutants, could be removed by constructed wetlands (CWs). While the traditional substrate of CWs has a weak adsorption capacity for PAHs, in this study, the carbonous fillers—activated carbon (AC) and biochar—were added into the substrate of surface flow CWs to improve the removal performance of benzofluoranthrene (BbFA), a typical PAH. The results showed that the BbFA removal efficiencies in CWs with the addition of AC and biochar were 11.8 and 1.2% higher than those in the Control group, respectively. Simultaneously, the removal efficiencies of NO3––N were 42.8 and 68.4% in these two CWs, while the BbFA content in the substrate and plants with the addition of carbon was lower than that in the Control group. The addition of carbonous filler reduced the absorption of PAHs by plants in CWs and enhanced microbial degradation. The microbial community results showed that the relative abundance of Proteobacteria, especially γ-proteobacteria, was higher with the addition of fillers, which related to PAH degradation.
研究领域:
环境科学
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水处理
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污染控制
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yaqin Wang,Lingjia Fan,Jintao Huang,Jingjing Liang,Xingnan Wang,Yichen Ren,Hongcai Li,Tianli Yue,Zhenpeng Gao
To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ). The number of viable bacteria in the juice reached 2.8 × 10 8 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β -carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity. The gut microbiota of mice shifted by increasing Lactobacillus and Bifidobacterium and protected the gut barrier by reducing Proteobacteria after 48 h of fermentation. Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.
研究领域:
食品科学
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微生物学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2023
·Vol. 13
·期 3
·451
·
IF 4.1
Fujian Qin,Jiankang Li,Tianxiao Mao,Shuo Feng,Jing Li,Maode Lai
2-hydroxybutyric acid (2HB) serves as an important regulatory factor in a variety of diseases. The circulating level of 2HB in serum is significantly higher in multiple diseases, such as cancer and type 2 diabetes (T2D). However, there is currently no systematic study on 2HB-producing bacteria that demonstrates whether gut bacteria contribute to the circulating 2HB pool. To address this question, we used BLASTP to reveal the taxonomic profiling of 2HB-producing bacteria in the human microbiome, which are mainly distributed in the phylum Proteobacteria and Firmicutes. In vitro experiments showed that most gut bacteria (21/32) have at least one path to produce 2HB, which includes Aspartic acid, methionine, threonine, and 2-aminobutyric acid. Particularly,Fusobacterium nucleatumhas the strongest ability to synthesize 2HB, which is sufficient to alter colon 2HB concentration in mice. Nevertheless, neither antibiotic (ABX) norFusobacterium nucleatumgavage significantly affected mouse serum 2HB levels during the time course of this study. Taken together, our study presents the profiles of 2HB-producing bacteria and demonstrates that gut microbiota was a major contributor to 2HB concentration in the intestinal lumen but a relatively minor contributor to serum 2HB concentration.Keywords:2-hydroxybutyric acid;gut microbiota;HMP;taxonomic identification;Fusobacterium nucleatum
研究领域:
微生物学
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分子生物学
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医学研究
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Congli Cui,Mingju Li,Xiaoyang He,Na Ji,Yang Qin,Lei Dai,Liu Xiong,Qingjie Sun
This research prepared functional-resistant starch capsules by constructing multiple calcium alginate shells around calcium alginate/starch beads. Scanning electron microscopy revealed a dense structure for each layer of the calcium alginate shell, tightly encapsulating the calcium alginate/starch beads. The rapidly digestive starch content of three-layer calcium alginate shelled starch capsules decreased to 0.50 ± 1.14%, while the resistant starch content increased to 78.25 ± 1.55%. The estimated glycemic index (eGI) experiment suggested that the two-layer shell capsules (eGI: 59.91) and Three-layer shell capsules (eGI: 47.75) were classified as low-glycemic index foods . Furthermore, calcium alginate/corn starch multilayer capsules showed reduced microbial gas production and increased concentrations of propionic acid , isobutyric acid, and valeric acid compared to lactulose in vitro fecal fermentation experiment. Moreover, three-layer capsules and beads promoted a relative abundance of Bacteroidetes but inhibited the reproduction of Proteobacteria and Actinobacteria compared with lactulose and corn starch. These results suggest that constructing multi-layered sodium alginate shells for starch beads can effectively increase the digestive resistance of starch within the capsules and control its fermentation in the colon. This study will have a profound impact on the development of RS1-type resistant starch and be beneficial in the design and production of functional foods for prevention and assistance in the treatment of chronic diseases such as diabetes and obesity.
研究领域:
食品科学
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营养学
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
·
IF 14.224
Anchen Liu,Wenting Lin,Ruiliang Ming,Wenqi Guan,Xinying Wang,Ningyi Hu,Yuan Ren
Identifying the attenuation characteristics of drugs in sewage and sewers is one of the important factors to improve the accuracy of wastewater-based epidemiology (WBE) application. In this study, 28 drugs including antidepressants, cardiovascular drugs, antihistamines, anticonvulsants and some of their human metabolites were chosen as the targets to study the hydrolysis , adsorption, and biodegradation at different temperatures in sewage and sewers. The interaction between drugs degradation and community structure of biofilm was also investigated. In the simulated sewers, the removal percentages of 12 parent or drug metabolites are 0–20%, such as demethylvenlafaxine, fluvoxamine, etc., which are highly stable chemicals and suitable to be chosen as biomarkers for WBE back-calculation under appropriate circumstances. Fourteen drugs including venlafaxine and citalopram have removal percentages of 20–60%. While paroxetine and sertraline, with removal percentage of 100%, are the most unstable and cannot be used as biomarkers. Among the 28 drugs, there are 25 drugs that have a higher loss rate in the aerobic sewer than that in the anaerobic sewer in this study. During drug exposure in anaerobic biofilms, species abundance first decreased and then increased. Species abundance and diversity in aerobic biofilm generally showed a decreasing trend. In addition, Proteobacteria and Spirochaetota were the dominant phyla in both sewers.
研究领域:
环境科学
·
微生物学
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药理学
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流行病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2023
·
IF 1.6
Jie Zheng,Hao Ding,Lirong Bai,Shuai Zhu,Hongyuan Hui,Xinyue Zhu,Wen Chen,Aijun Hu
The Chinese traditional food, fermented semi-dried sea bass (FSSB), is famous for its unique flavor. The microbiota succession and flavor formation during fermentation remain unknown. This study utilized high-throughput sequencing to investigate the evolving microbiota and chemical determination to explore volatile compounds. The relevant abundance of microorganisms in the middle-fermented stage was significantly (p <.05) higher than that of the post-fermented stage. In addition, the main microbial phyla during fermentation were Firmicutes and Proteobacteria. In conclusion, this study contributes to understanding the changes in flavor substances during fermentation and the varying patterns of change among different floras.
研究领域:
食品科学
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微生物学
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化学分析
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Xinyue Zhang,Tao Lin,Fuchun Jiang,Xue Zhang,Shiyu Wang,Shisheng Zhang
Pipe material and residual chlorine are key factors for the drinking water distribution system, and understanding the biofilm ecosystem is vital for water quality safeguard. The aim of our study was to determine the influence of pipe materials (ductile iron, steel, polyethylene) and chlorination on the biofilm structure and microbial community , as shown by the physicochemical properties , extracellular polymeric substances (EPS) structural characteristics, bacterial community composition, and functional traits. EPS spatial properties were studied based on a semi-quantitative confocal laser scanning microscope (CLSM) description. Regarding the impact of chlorination, residule chlorine (1.0 ± 0.3 mg L −1 free chlorine) could inhibit the bacteria colonization, and initiate a potential response to external disinfectants revealed by the EPS spatial distribution changes and communities variation compared to unchlorinated system. Regarding the impact of pipe material, polyethylene (PE) biofilms displayed lower biomass, loose zoogloea structure, lower proteins and polysaccharides content, and poor microbial diversity in contrast to ductile iron and steel biofilms. Pipe material was the more possible driving factor of the biofilm community composition compared to the chlorination based on principal coordinates analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA). Actinobacteria was dominant in the PE biofilms (45.57%–83.32%), while Alphaproteobacteria (34.30%–73.22%) and Gammaproteobacteria (6.46%–36.82%) were the major classes in the steel and ductile iron biofilms. The genus Rhodococcus was predominant in the PE biofilms. Rhodococcus , Pseudomonas , and Sphingomonas seemed to have a better growth advantage in the chlorinated system and display a stronger disinfectant resistance. Functional sketch prediction indicated the potential impact of pipe material and chlorination on functional pathway abundnce, possible functional pathways associated with infectious disease included. This study provides insights into the impact of pipe material and chlorination on biofilm structure and microbial community and might help to develop monitoring or maintenance strategies to protect the biosafety of the drinking water.
研究领域:
环境科学
·
微生物学
·
水利工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Zhenhua Yin,Juanjuan Zhang,Qingfeng Guo,Jiale Zhi,Xia Zhang,Wei Zhang,Lin Chen
Psoralea corylifolia polysaccharide (PPs) was the bioactive ingredient that conferred health benefits. In this study, simulated digestion and anaerobic fermentation models were employed to explore the digestion and fermentation characteristics of PPs and its effect on the intestinal microbiota. The results revealed that while the total and reducing sugar contents of PPs increased after simulated digestion, their molecular weight, monosaccharide composition, and overall structure remained unchanged, indicating that PPs was not degraded during digestion. On the other hand, during anaerobic fermentation, PPs was metabolized by intestinal microbiota, leading to the production of acetic acid and propionic acid, which subsequently reduced the pH of the fecal culture solution. Additionally, PPs modulated the composition of the intestinal flora, significantly decreasing the relative abundance of Proteobacteria while increasing the relative abundance of Bacteroidota and Firmicutes to varying degrees. This study highlighted the potential of PPs for development as functional foods and therapeutic agents.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Yanping Hou,Guiyun Yuan,Shanming Qin,Lingli Tu,Yimin Yan,Zebin Yu,Hongfei Lin,Yongli Chen,Hongxiang Zhu,Hainong Song,Shuangfei Wang
To further enhance the bio-photoelectrochemical system (BPES) performance for nitrofurazone (NFZ) degradation and current output, the g-C 3 N 4 /CdS photocathode was optimized, and microbial community shift from inoculation to the BPES was analyzed. Results showed that photocathode with g-C 3 N 4 /CdS (mass ratio of 1:9) loading of 7.5 mg/cm 2 exhibited the best performance, with NFZ removal of 83.14% (within 4 h) and current of ~9 mA in the BPES. Proteobacteria accounted for the largest proportion: 66.53% (inoculation), 71.89% (microbial electrolysis cell (MEC) anode), 74.67% (BPES anode) and 57.31% (BPES cathode), respectively. In addition, Geobacter was the most dominant genus in MEC and BPES anode and cathode, which occupied 31.64%, 67.73% and 41.34%, respectively. The microbial compositions of BPES anode and cathode were similar, but different from that of MEC anode. Notably, Rhodopseudomonas , a photosynthetic species, was detected in the BPES. Cognition of microbial community in the BPES is important for advancing its development.
研究领域:
环境科学
·
微生物学
·
电化学
·
水处理
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 12
·2791
·
IF 4.2
Haijun Xu,Zhu Liu,Wen Xu,Yafei Zhang
The extract ofDendrobium huoshanense, a traditional Chinese medicinal and food homologous plant belonging to the family Orchidaceae, was previously reported to have hypoglycemic and antioxidant effects. In this study, the direct effects of polysaccharide (DHP) and non-polysaccharide (NDHP) components ofD. huoshanense, as well as its water extract (DHWE) were compared with that of metformin (an antidiabetic drug) on the gut microbiota (collected from fecal flora) of rats with streptozotocin-induced type 1 diabetes (T1D) using an in vitro fermentation method. The results showed that DHWE, DHP, and NDHP reduced pH and increased bacterial proliferation and short-chain fatty acid (SCFA) content in fermentation broth. DHWE, DHP, NDHP and metformin promoted the production of acetic and propionic acid, acetic acid, propionic acid and butyric acid, and propionic acid, respectively. DHWE, DHP, and NDHP reduced the abundance ofProteobacteria(subdominant pathogenic bacteria) and increased the abundance ofFirmicutes(dominant beneficial gut bacteria). NDHP also reduced the abundance ofBacteroidetes(beneficial and conditional pathogenic). Metformin increased the abundance ofProteobacteriaand reduced the abundance ofFirmicutesandBacteroidetes. At the genus level, NDHP promoted the proliferation ofMegamonasandMegasphaeraand decreased harmful bacteria (e.g.,Klebsiella), and DHP increased the abundance ofPrevotellaceae(opportunistic and usually harmless). By contrast, metformin increased the abundance of harmful bacteria (e.g.,Citrobacter) and reduced the abundance of beneficial bacteria (e.g.,Oscillospira). Our study indicates that DHWE, DHP, and NDHP are potentially more beneficial than metformin on the gut microbiota of T1D rats in vitro.
研究领域:
药理学
·
微生物学
·
糖尿病研究
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）