RSC Advances
·
2018
·Vol. 8
·期 35
·19642-19650
·
IF 2.936
Fancui Meng,Zhuangzhuang Qiao,Yan Yao,Jianbin Luo
Polyurethane with pendant azide groups on the soft segment (PU-GAP) was prepared in this study to further increase the content of reactive azide groups and improve their surfaces enrichment for further functionalization. Polymer diols with pendant azide groups (GAP) were prepared by transforming the pendant chlorine groups at polyepichlorohydrin (PECH) into azide groups with sodium azide. The prepared PECH, GAP and PU-GAP was characterized by GPC, 1H NMR and FTIR. Propargylic mPEG (mPEG-alkyne) was used as model surface modification reagents which was grafted on the prepared azido containing polyurethane films via click chemistry. The surface morphology, chemical composition and wettabilities were studied by SEM, XPS and water contact angle (WCA) analysis, respectively. SEM results demonstrated different surface topologies between mPEG modified PU surface and original PU surface. XPS and WCA analysis proved the successful grafting of mPEG on the pendant azide groups of PUs. The mPEG modified PU surfaces demonstrated good antifouling activities against model bacteria and mPEG with larger molecular weights modified surfaces showed better resistance efficiency to attachment of bacteria. Therefore, the surface reactive polyurethane we prepared can be a universal platform for further functionalization according actual applications.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2020
·Vol. 27
·期 6
·1-11
·
IF 2.426
Qiu Xibo,Zhai Hong,Li Longfei,Dong Peng,Bai Ying,Peng Ai-Yun,Quan Daping
The intention of this work was to study the polymerization of a cyclic carbonate monomer bearing one azido group, i.e. 2-(azidemethyl)-2-methyltrimethylene carbonate (AMTC), to disclose the characteristics of the resulted polymers and to explore the feasibility of their post-functionalization with alkynyl compounds via Click reaction. Homopolymerization of AMTC was first studied using stannous octoate (Sn(Oct) 2 ) as the catalyst and dodecanol as the initiator, which had the characteristic of active polymerization. Copolymerization of AMTC with L -lactide ( L -LA) was then investigated under the same reaction condition, in which L- LA exhibited higher reactivity than AMTC. The reactivity of AMTC was low not only in its homopolymerization but also in its copolymerization with L -LA, while the conversion of AMTC increased by extending the reaction time. The copolymer P(AMTC-co- L LA) (PLAC) had the semicrystalline characteristic due to the relative long block-like segments of P L LA ( \( {\overline{L}}_{\mathrm{L}}=35.5 \) ). Based on these results, copolymerization of AMTC with L -LA using PEG 4K as macroinitiator was further studied to afford PLAC-PEG-PLAC copolymers. The copolymerization could be well controlled at low ratio of [M] 0 /[I] 0 (from 10 to 50) and molar fraction of AMTC in feed ( f AMTC = 0.1, 0.2). The pendent azido groups of PLAC-PEG-PLAC could undergo post-functionalization through azide-alkyne click reaction easily and efficiently.
研究领域:
高分子化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Jing Hu,Yu-Hang Zhang,Wei Chen,Zheng-Wu Bai
In order to covert azidoamylose into ureidoamylose derivatives in a facile way, 6-azido-6-deoxyamylose was reacted with triphenylphosphine , and then with water and a phenyl isocyanate in turn. The obtained product was amylose 2,3-bis(phenylcarbamate)-6-deoxy-6-(phenylurea), which was synthesized through three reactions in a one-pot method. The essential point to ensure the method be successful was that the water should be fed at an appropriate amount. The prepared amylose 2,3-bis(phenylcarbamate)-6-deoxy-6-(phenylurea)s were characterized by FT-IR, 1 H NMR and elemental analysis , substantiating the reduction of the azido group and the subsequent derivatization of the resulted amino group and the hydroxyl group in amylose were fully performed. In addition, the amylose 2,3-bis(phenylcarbamate)-6-deoxy-6-(phenylurea)s were coated on macroporous 3-aminopropyl silica gel to afford chiral stationary phases (CSPs) for high-performance liquid chromatography. The enantioseparations of the CSPs were evaluated, and the results indicated that the CSPs possessed chiral recognition ability towards some chiral compounds.
研究领域:
有机化学
·
高分子化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 25
·11380-11389
·
IF 3.591
Rui Yang,Yifei Liu,Zhen Dong,Haiyan Li,Zhiwen Ye
As an environmentally friendly energetic group, the azide group can not only control the melting point but also increase the energy of the compound. Therefore, the design and synthesis of energetic compounds with azido groups have long been a focus of research in the field of energetic materials. 3-R-4-(5-methyleneazide-1,2,4-oxadiazol-3-yl)furazan energetic materials were synthesized and fully characterized. Using computational chemistry methods, the interactions of some compounds were studied, and the physical and chemical properties of all of compounds were examined. The results show that 3-amino-4-(5-methyleneazide-1,2,4-oxadiazol-3-yl)furazan (2) has a melting point similar to that of TNT and can be a potential carrier for molten-cast explosives. Hydroxylamine salt (4) has an excellent detonation velocity and detonation pressure of 9090 m s−1 and 35.76 GPa, respectively, and its energy is significantly higher than that of RDX, enabling replacement of RDX as a high-energy explosive. Although the detonation performance of compound 4 is slightly lower than that of HMX, its sensitivity is better than that of HMX, which shows that compound 4 still has a high application value. Non-covalent interactions (NCIs) and Hirshfeld surface models indicate that hydrogen bonding and π–π interactions are the key factors that reduce the sensitivity of compounds. Therefore, our results for 3-R-4-(5-methyleneazide-1,2,4-oxadiazol-3-yl)furazan energetic materials suggest that it may be competitive for application in both molten-cast explosives and high-energy explosives.
研究领域:
化学
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材料科学
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爆炸物研究
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Zhanwu Hou,Xiao Han,Zhen Wang,Sana Ghazanfar,Jeffy Yang,Huadong Liu
Nascent proteome presents dynamic changes in response to a certain stimulus. Thus, monitoring nascent proteome is critical to uncovering the involved biological mechanism. But the low-abundance of nascent proteome against an overwhelming pre-existing proteome limits its identification and quantification. Herein, we present a novel strategy to enrich nascent proteome from whole cell lysate for further analysis by mass spectrometry. We employed a terminal alkyne and disulfide functionalized agarose resin to capture nascent proteome which had been labeled by L-azidohomoalanine. Results from the western blot, silver staining and pulse metabolic labeling suggested that the nascent proteome could be enriched efficiently. Applied to Hela cells, the method identified about 700 nascent proteins with good correlation with previous reports. The above indicates that our strategy can be used to reveal the proteome dynamics of biological processes.
研究领域:
蛋白质组学
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细胞生物学
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生物化学
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质谱
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产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2020
·Vol. 221
·期 18
·2000157
·
IF 2.335
Ming Gao,Hang Lu,Rong-Hao Song,Lin Ye,Ai-Ying Zhang,Zeng-Guo Feng
To highlight whether γ-cyclodextrins (CDs) facilitate propargylamine monosubstituted β-CDs (PA-β-CDs) as end stoppers to get threaded onto a distal azide terminated poly(2-hydroxyethylmethacrylate) (PHEMA) homopolymer (PH-46-2N 3 ) to create linear and hyperbranched polyrotaxanes (PRs) via the in situ copper-catalyzed azide/alkyne cycloaddition (CuAAC), PH-46-2N 3 is self-assembled with a varying amount of γ-CDs in water and then subjected the CuAAC with PA-β-CDs to end-cap the resulting γ-CD-PHEMA polypseudorotaxanes (PPRs) into the γ-CD-PHEMA PRs. It demonstrates that γ-CDs cannot promote PA-β-CDs to be entrapped on the PHEMA chain most likely due to their different cavity size and molecular framework and linear PRs are always formed with up to 29% γ-CD coverage ratio along the PHEMA axis thereof.
研究领域:
高分子化学
·
超分子化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Min Chen,Yang Li,Rui Han,Qiuji Chen,Lingyun Jiang,Xiliang Luo
Herein, an electrochemical immunosensor capable of detecting targets in human serum with ultralow fouling and high sensitivity was successfully constructed through a click reaction. One designed biotinylated peptide was associated with the other peptide functionalized with biotin and azide groups at its two terminals, to form a branched antifouling peptide through the streptavidin-biotin affinity interaction, and it was attached to the electrode to construct an antifouling interface. Then, the exposed azide group on the branched peptide was used to link the antibody modified with 5′-dibenzocyclooctyne (DBCO), and the immunosensor was thus constructed with the aid of the click reaction between the azide group and the DBCO. The developed antifouling immunosensor demonstrated a wide linear response range for carcinoembryonic antigen (CEA) and a low limit of detection of 40 fg mL −1 , which is much superior to the sensing performance of the immunosensor prepared through the normal EDC/NHS method. The immunosensor was capable of assaying CEA in undiluted serum samples with satisfying accuracy when compared with a clinically approved method. This work offered an effective strategy to develop various biosensors through the conjugation of antifouling peptides with different antibodies.
研究领域:
分析化学
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生物化学
·
生物传感器
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电化学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2023
·
IF 4.6
Ming Gao,Chun-jiang He,Xue Geng,Lin Ye,Ai-ying Zhang,Zeng-guo Feng
When γ-CDs were self-assembled with a distal 2-chloropropionyl terminated poly (ethylene glycol) (PEG-2Cl) in aqueous solution, two distinct polypseudorotaxanes (PPRs), a single stranded one and a double stranded one were identified from the upper clear solution and the lower precipitates, respectively. Moreover, if the chlorine groups in PEG-2Cl were converted into the azido groups to give a bifunctional azido terminated PEG (PEG-2N 3 ) and then subjected to the inclusion complexation with γ-CDs, two similar double stranded PPRs were lyophilized from the upper and lower layer products. To probe whether the parallel or bent double stranded PPRs were created from the self-assembly of γ-CDs with PEG-2N 3 , the in situ copper-catalyzed azide/alkyne cycloaddition (CuAAC) was conducted to transform the resulting PPRs into the PRs using propargylamine monosubstituted β-CDs (PA-β-CDs) as bulky stoppers. After thorough purification, both the upper and lower layer products lyophilized therefrom were revealed the same parallel double stranded PRs by spectral and thermal analyses.
研究领域:
材料科学
·
聚合物化学
·
超分子化学
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Lei Luo,Fei Liu,Zhiying Li,Siyuan Yue,Liang Wang,Shiling Zhang,Shanmeng Lin,Jinyan Luo,Meng Wang,Yichuan Zhang,Mohamed Abdelrahim,Qi Xing,Jin Geng
Nanoparticles (NPs) containing light-responsive polymers and imaging agents show great promise for controlled drug delivery. However, most light-responsive NPs rely on short-wavelength excitation, resulting in poor tissue penetration and potential cytotoxicity. Moreover, excessively sensitive NPs may prematurely release drugs during storage and circulation, diminishing their efficacy and causing off-target toxicity. Herein, we report visible-light-responsive NPs composed of an amphiphilic block copolymer containing responsive 4-acrylamide benzenesulfonyl azide (ABSA) and hydrophilic N,N′-dimethylacrylamide (DMA) units. The polymer pDMA-ABSA was loaded with the chemotherapy drug dasatinib and zinc tetraphenylporphyrin (ZnTPP). ZnTPP acted as an imaging reagent and a photosensitizer to reduce ABSA upon visible light irradiation, converting hydrophobic units to hydrophilic units and disrupting NPs to trigger drug release. These NPs enabled real-time fluorescence imaging in cells and exhibited synergistic chemophotodynamic therapy against multiple cancer cell lines. Our light-responsive NP platform holds great promise for controlled drug delivery and cancer theranostics, circumventing the limitations of traditional photosensitive nanosystems.
研究领域:
材料科学
·
药学
·
生物医学工程
·
纳米技术
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Junbin Liao,Xinyan Yu,Nengxiu Pan,Jun Li,Jiangnan Shen,Congjie Gao
Fabricating stable mono-/multi-valent anion-selective membranes with high perm-selectivity for electrodialysis (ED) applications is critical but challengeable. In this work, we have fabricated a series of novel homogeneous monovalent anion-selective amphoteric ion-exchange membranes (AIEMs, PAES-im-Xc) based on a long-side-chain type imidazolium-functionalized poly(arylene ether sulfone) cross-linked with 4,4′-diazostilbene-2,2′-disulfonic acid disodium salt (DAS) for ED. By tuning the mass percent of DAS added, low swelling ratio but appreciably high anion perm-selectivity (in Cl – /SO 4 2– system) of optimized AIEM are achieved to values of 8.0% and 47.12 at current density of 2.5 mA cm –2 , respectively. In particular, at an enhanced current density of 5.0 mA cm –2 , its perm-selectivity reaches 12.5, significantly outperforming commercial monovalent anion-selective Neosepta ACS (5.27). The appreciably high perm-selectivity possibly arises from pore-size sieving effect from dense matrix structure with cross-linkings and strong electrostatic repulsion between negative fixed –SO 3 – groups and negative divalent ions of SO 4 2– in feeding solution. The superior performances might be particularly interesting findings that can facilitate the development of advanced mono-/bi-valent anion-selective membranes in applications of sea water desalination, resource recovery and water treatment/pretreatment etc..
研究领域:
材料科学
·
环境工程
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
Food & Nutrition Research
·
2022
·
IF 3.221
Tian Su,Zhen Wang,Zhengyi Zhang,Zhanwu Hou,Xiao Han,Fei Yang,Huadong Liu
Background Resveratrol, a well-known natural compound and nutrient, activates the deacetylation ability of SIRT1, demonstrating p53-dependent apoptosis functions in many diseases. However, the nascent proteomic fluctuation caused by resveratrol is still unclear. Objective In this study, we investigated the effect of resveratrol on the nascent proteome and transcriptome initiated by SIRT1 activation, and we explored the mechanism of resveratrol in HEK 293T cells. Methods Bioorthogonal noncanonical amino acid tagging (BONCAT) is a method used to metabolically label nascent proteins. In this strategy, L-azidohomoalanine (AHA) was used to replace methionine (Met) under different conditions. Taking advantage of the click reaction between AHA and terminal alkyne- and disulfide-functionalized agarose resin (TAD resin), we were able to efficiently separate stimulation responsive proteins from the pre-existing proteome. Resveratrol responsive proteins were identified by Liquid Chromatograph-Mass Spectrometer/Mass Spectrometer (LC-MS/MS). Furthermore, changes in mRNA levels were analyzed by transcriptome sequencing. Results Integrational analysis revealed a resveratrol response in HEK 293T cells and showed that Hsp60 was downregulated at both the nascent protein and mRNA levels. Knockdown of SIRT1 and Hsp60 provides evidence that resveratrol downregulated Hsp60 through SIRT1 and that Hsp60 decreased p53 through the Akt pathway. Conclusions This study revealed dynamic changes in the nascent proteome and transcriptome in response to resveratrol in HEK 293T cells and demonstrated that resveratrol downregulates Hsp60 by activating SIRT1, which may be a possible mechanism by which resveratrol prevents p53-dependent apoptosis by regulating Hsp60.
研究领域:
分子生物学
·
细胞生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2022
·
IF 0
Haili Zhao,Tao Chen,Tong Wu,Linsheng Xie,Yulu Ma,Jin Sha
Functional surfaces that enable both spatial and temporal control of biomolecules immobilization have attracted enormous attention for various fields including smart biointerface materials, high-throughput bioarrays, and fundamental research in the biosciences. Here, a flexible and promising method was presented for regulating the spatiotemporal arrangement of multiple biomolecules by constructing the topographically and chemically diverse polymer brushes patterned surfaces. A series of polymer brushes patterned surfaces, including antifouling brushes patterned surface, epoxy-presenting brushes patterned surface without and with antifouling background layer, were fabricated to control the spatial distribution of protein and cell adhesion through specific and nonspecific means. The fluorescence measurements demonstrated the effectiveness of spatially regulating the density of surface-immobilized protein through controlling the areal thickness of the poly (glycidyl methacrylate) (PGMA) brush patterns, leading to various complex patterns featuring well-defined biomolecule concentration gradients. Furthermore, a multiplexed surface bearing epoxy groups and azido groups with various areal densities was fabricated for regulating the spatiotemporal arrangement of different proteins, enabling binary biomolecules patterns with higher degrees of functionality and complexity. The presented strategy for the spatiotemporal control of biomolecules immobilization would boost the development of dynamic and multifunctional biosystems.
研究领域:
智能生物界面材料
·
高通量生物芯片
·
生物科学
✓ 已验证
DOI
PubMed
危险品化学品经营许可证（带存储）