CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Qimeng Zhang,Mingzheng Wang,Guangqin Mu,Haotian Ren,Congshuang He,Qian Xie,Quanxiao Liu,Jigang Wang,Ruitao Cha
Nanocellulose has gained much attention because of its excellent properties. Cationic cellulose nanocrystals (cCNC) shows good adsorptivity toward negative ions and molecules. Phosphate binders are most used to treat hyperphosphatemia and it is significant to develop its alternatives with high specific and low cost in the clinic. Herein, we prepared cCNC and characterized it by FTIR, TEM, dynamic light scattering, and viscosity method. We simulated the binding process of cationic cellulose for phosphate and used it as phosphate binder for hyperphosphatemia therapy to study the phosphate binding effect and evaluate the oral toxicity. Cationic cellulose improved the conditions of mice models and efficiently decreased the level of phosphate in the serum. cCNC had a better binding effect than cationic microcrystalline cellulose both in vitro and in vivo . cCNC could be used as alternatives to phosphate binder for therapy of chronic renal failure and hyperphosphatemia.
研究领域:
材料科学
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医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 2
·293
·
IF 5.2
Pengbo Wang,Dongman Wan,Ting Peng,Yujing Yang,Xuefang Wen,Xianghui Yan,Jiaheng Xia,Qingwen Zhu,Ping Yu,Deming Gong,Zheling Zeng
Cinnamomum camphoraseed kernel oil (CCSKO) is one of the important natural medium chain triglycerides (MCT) resources, with more than 95.00% of medium chain fatty acids found in the world, and has various physiological effects. However, CCSKO has not been generally recognized as a safe oil or new food resource yet. The acute oral toxicity test and a standard battery of genotoxicity tests (mammalian erythrocyte micronucleus test, Ames test, and in vitro mammalian cell TK gene mutation test) of CCSKO as a new edible plant oil were used in the study. The results of the acute oral toxicity test showed that CCSKO was preliminary non-toxic, with an LD50value higher than 21.5 g/kg body weight. In the mammalian erythrocyte micronucleus test, there was no concentration-response relationship between the dose of CCSKO and micronucleus value in polychromatic erythrocytes compared to the negative control group. No genotoxicity was observed in the Ames test in the presence or absence of S9 at 5000 μg/mL. In vitro mammalian cell TK gene mutation test showed that CCSKO did not induce in vitro mammalian cell TK gene mutation in the presence or absence of S9 at 5000 μg/mL. These results indicated that CCSKO is a non-toxic natural medium-chain oil.Keywords:Cinnamomum camphoraseed kernel oil;acute oral toxicity;genotoxicity assessment;medium chain oil;safety assessment
研究领域:
毒理学
·
食品科学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NanoImpact
·
2025
·
IF 5.5
Yun Wang,Chen Li,Te Ba,Shengyuan Wang,Langzhi He,Zhangjian Chen,Ji Pu,Xiaoxing Cui,Guang Jia
Titanium dioxide nanoparticles (TiO 2 NPs) have promising applications in food additives and pharmaceutical dressings, raising concerns about their oral safety. The current studies mainly focus on healthy groups, and the effect of TiO 2 NPs on the patient population is rarely known. Here, a comprehensive toxicity study of TiO 2 NPs (75 ± 15 nm, anatase) in gastric ulcer rats ( male 8-week old Sprague-Dawley rats) is reported following oral exposure at dose of 0, 10, 50, 200 mg/kg body weight per day for 30 days. The gastric ulcer rats were produced by submucosal injection of acetic acid solution into the rat stomach. The healthy rats were used as the normal control. We evaluated nanoparticle biodistribution, systemic toxicity, and gastrointestinal function indices in the rats. Our findings indicate that oral administration of TiO 2 NPs resulted in minimal intestinal absorption and transport with limited systemic organ toxicity. The internalization of TiO2 NPs and activation of mast cells in the stomach tissues, along with the low serum levels of histamine and IgE, suggest a localized allergic reaction rather than a systemic one. Furthermore, the notably reduced plasma levels of D-lactate and the activity of diamine oxidase (DAO) indicated the upregulation of intestinal barrier function. These statistically significant results indicated that gastrointestinal dysfunction was the main performance of the oral toxicity of TiO 2 NPs on gastric ulcer rats, emphasizing the importance of controlling the intake of TiO 2 NPs in patients with gastric ulcers.
研究领域:
毒理学
·
消化病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2012
·
IF 8.349
Yun Wang,Zhangjian Chen,Te Ba,Ji Pu,Tian Chen,Yanshuang Song,Yongen Gu,Qin Qian,Yingying Xu,Kun Xiang,Haifang Wang,Guang Jia
Titanium dioxide nanoparticles (TiO 2 NPs) have potential applications as food additives, but concerns persist about their safety. Children are identified as having the highest exposure and may face the greatest health risks. However, the toxicological sensitivity of TiO 2 NPs in different ages is not clear. Here, a comparative toxicity study of TiO 2 NPs in 3-week (youth) and 8-week (adult) old Sprague-Dawley rats is reported following oral exposure at doses of 0, 10, 50, 200 mg kg −1 body weight per day for 30 days. The organ mass and histology, blood biochemistry and redox state, intestinal function, and biodistribution of NPs are characterized. The results show that TiO 2 NPs induce different toxic effects on young and adult rats. The liver edema, heart injuries and non-allergic mast cell activation in stomach tissues are found in young rats. On the other hand, only slight injury in the liver and kidney and decreased intestinal permeability and molybdenum contents are found in adult rats. Furthermore, TiO 2 NP exposure can provoke reductive stress (i.e., increased reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios) in plasmas through enhancing the glucose and GSH levels in young rats or reducing the glutathione peroxidase (GSH-Px) acitivity and GSSG levels in adult rats. These results suggest that different ages may require different biomarkers for identifying and monitoring oral toxicity of nanoparticles.
研究领域:
毒理学
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纳米生物学
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公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Bilang Cao,Qiang Zhang,Juan Guo,Ruixue Guo,Xiaodan Fan,Yongguang Bi
Grateloupia Livida polysaccharides-functionalized selenium nanoparticles (GLP-SeNPs) have been successfully prepared in a simple redox system of sodium selenite and ascorbic acid. The size, morphology, structure, stability and thermal behavior were analyzed by various characterization methods. These results showed that, GLP-SeNPs (particle size of 115.54 nm) prepared in optimal synthesis conditions (temperature of 45 °C, reaction time of 3 h, GLP concentration of 1.0 mg/mL and ascorbic acid concentration of 0.04 M) obtained by orthogonal experiments were uniform spherical and could be stable for 30 days at 4 °C. GLP-SeNPs exhibited significant scavenging ability on DPPH, ABTS, hydroxyl radical and superoxide anion radical when compared to GLP and Na 2 SeO 3 . GLP-SeNPs showed selective cytotoxicity toward various human cancer cells, but not normal cells. Besides, GLP-SeNPs exhibited low oral acute toxicity. Taken together, GLP-SeNPs might be used as potential diet nutritional supplement or anticancer agent.
研究领域:
纳米技术
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药学
·
生物医学工程
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癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
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2016
·
IF 3.205
Bin Liu,Yanfei Liu,Jie Chai,Xiangquan Hu,Duoming Wu,Binsheng Yang
As a man-made additive, chromium picolinate Cr(pic) 3 has become a popular dietary supplement worldwide. In this paper Cr(pic) 3 and its new derivatives Cr(6-CH 3 -pic) 3 ( 1 ), [Cr(6-NH 2 -pic) 2 (H 2 O) 2 ]NO 3 ( 2 ) and Cr(3-NH 2 -pic) 3 ( 3 ) were synthesized, and complexes 1 and 2 were characterized by X-ray crystal structure (where pic = 2-carboxypyridine). The relationship between the chemical properties and biotoxicity of these complexes was fully discussed: (1) The dynamics stability of chromium picolinate complexes mainly depends on the Cr N bonds length. (2) There is a positive correlation between the dynamics stability, electrochemical potentials and generation of reactive oxygen species through Fenton-like reaction. (3) However, no biological toxicity was observed through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and sub-chronic oral toxicity study for these chromium picolinate compounds. Together, our findings establish a framework for understanding the structure-property-toxicity relationships of the chromium picolinate complexes.
研究领域:
化学
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毒理学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
REGULATORY TOXICOLOGY AND PHARMACOLOGY
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2016
·
IF 2.227
Xin Liu,Liangqing Zheng,Rong Zhang,Gang Liu,Shensheng Xiao,Xiaoting Qiao,Yongning Wu,Zhiyong Gong
Nε-(carboxymethyl)lysine (CML) as a novel potential noxious compound in various food products has aroused extensive concern in recent years. This study aimed to investigate the oral acute and subacute toxicity of CML in mice as per OECD 420 and 407 guidelines. Acute administration of 2000 and 5000 mg/kg CML did not induce any mortality within 14 days, nevertheless some toxicological symptoms and histopathological changes were observed. The estimated LD 50 of CML was >5000 mg/kg. In subacute toxicity test , CML was dosed at 200, 500 and 1000 mg/kg in both genders for 28 days. The body weights reduced which was accompanied with the decrease of food consumptions. Hematology parameters viz. RBC , HGB and MCH showed minor alteration but these were still within normal range. Biochemical analysis of hepatic and renal function markers showed significant elevating in AST , ALT , Cr and BUN etc. Histopathological alterations were observed in lung, liver, kidney and spleen. Subacute toxicity of CML involved oxidative stress caused by reducing antioxidant enzyme (SOD and GSH-Px) activities, and significantly increasing lipid peroxide (MDA) level. In conclusion, CML was unlikely to present an acute hazard, but repeated administration could produce deleterious effects on mice especially inducing liver and kidney damage through oxidative stress.
研究领域:
毒理学
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食品科学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Hong Lin,Jianbin Tan,Jing Wang,Chengliang Xie,Bifeng Chen,Mansi Luo,Yun Liu,Wenzhen Liao,Weiling Huang,Hongxia Wang,Ying Jiang,Kexin Wang,Ciyong Lu,Min Zhao
The widespread use of titanium dioxide nanoparticles (TiO2 NPs) in the food industry has brought about human safety risks related to nanotoxicity. In this study, food-related TiO2 NPs (anatase, 40 nm) were given to rats by oral gavage for 90 days at doses of 10, 100, and 1000 mg/kg bw. An additional two satellite groups underwent the same protocol for 45 days and for 90 days followed by a 28 day recovery. TiO2 NPs tended to agglomerate together in H2O, AGJ, and AIJ. No systemic toxicity was observed after 90 day agglomerated TiO2 NP exposure with no Ti distribution in major tissues/organs. Furthermore, TiO2 NP consumption for 90 days had no impact on microbiota diversity; the community structure of the gut microbiota is shifted to some extent at the genus level. Collectively, the NOAEL of agglomerated TiO2 NPs for 90 days of oral administration was 1000 mg/kg bw, the highest dose tested in male and female rats.
研究领域:
毒理学
·
纳米技术
·
食品安全
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2024
·
IF 4.7
Hong Ding,Xuanxiao Lu,Xiaoguo Ji,Shijie Wang,Jiayang Jin,Mengyao Zhao,Xiaofeng Hang,Liming Zhao
Selenium supplements are beneficial to human health, however, concerns regarding the toxicity of inorganic selenium have stimulated research on safer organic compounds. The main objective of this study was to develop a novel glucosamine-selenium compound (Se-GlcN), clarify its structure, and subsequently investigate its oral toxicity and in vitro anti-hepatitis B virus (HBV) activity. Electron microscopy, infrared, ultraviolet spectroscopy, nuclear magnetic resonance and thermogravimetric analyses revealed a unique binding mode of Se-GlcN, with the introduction of the Se-O bond at the C6 position, resulting in the formation of two carboxyl groups. In acute toxicity studies, the median lethal dose (LD 50 ) of Se-GlcN in ICR mice was 92.31 mg/kg body weight (BW), with a 95% confidence interval of 81.88 to 104.07 mg/kg BW. A 30-day subchronic toxicity study showed that 46.16 mg/kg BW Se-GlcN caused livers and kidneys damage in mice, whereas doses of 9.23 mg/kg BW and lower were safe for the livers and kidneys. In vitro studies, Se-GlcN at 1.25 μg/mL exhibited good anti-HBV activity, significantly reducing HBsAg, HBeAg, 3.5 kb HBV RNA and total HBV RNA by 45%, 54%, 84%, 87%, respectively. In conclusion, the Se-GlcN synthesized in this study provides potential possibilities and theoretical references for its use as an organic selenium supplement.
研究领域:
药物化学
·
毒理学
·
病毒学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-15
·
IF 3.8
Wu Ying,Wu Zhiyi,Gao Yinyin,Fan Yixuan,Dong Yao,Zhang Yinan,Gai Zhonghui,Gu Shaobin
This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods and pharmaceuticals. We began with whole genome sequencing of BC99, followed by a comprehensive safety assessment comprising genome analysis, hemolysis, cytotoxicity, antibiotic susceptibility tests, and cell adhesion and tolerance studies, along with acute and subacute oral toxicity studies in animal models. BC99 was isolated from a well-characterized collection originating from the feces of a healthy infant. Our results indicated no hemolytic activity on Columbia blood agar plates and broad antibiotic sensitivity, including to gentamicin, ampicillin, chloramphenicol, ciprofloxacin, and others. Cytotoxicity testing confirmed no adverse effects on HT-29 cells and significant adhesive properties to intestinal epithelial cells. Tolerance tests demonstrated over 90% viability of BC99 under simulated gastrointestinal conditions. In vivo studies in mice and rats confirmed the absence of adverse effects following oral administration. Collectively, these findings support BC99’s robust tolerance to gastrointestinal environments, strong adhesion capabilities, and a broad spectrum of antibiotic resistance, underlining its potential as a safe and effective agent for gut microbiota modulation and host health enhancement.
研究领域:
微生物学
·
基因组学
·
毒理学
·
药理学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
·
2014
·
IF 2.491
Hai-yan Tang,Qing-gui Xiao,Hong-bin Xu,Yi Zhang
The hypoglycemic activity of chromium methionine (CrMet) in alloxan-induced diabetic (AID) mice was investigated and compared with those of chromium trichloride hexahydrate (CrCl 3 ·6H 2 O) and chromium nicotinate (CrNic) through a 15-day feeding experiment. The acute oral toxicity of CrMet was also investigated in ICR (Institute for Cancer Research) mice by a single oral gavage. The anti-diabetic activity of CrMet was explored in detail from the aspects of body weight (BW), blood glucose, triglyceride, total cholesterol, liver glycogen levels, aspartate transaminase (AST) and alanine transaminase (ALT) levels. The obtained results showed that CrMet had beneficial effects on glucose and lipid metabolism, and might possess hepatoprotective efficacy for diabetes. Daily treatment with 500 and 1000 μg Cr/kg BW of CrMet in AID mice for 15 days indicated that this low-molecular-weight organic chromium complex had better bioavailability and more beneficial effects on diabetics than CrCl 3 ·6H 2 O. CrMet also had advantage over CrNic in the control of AST and ALT activities. Acute toxicity studies revealed that CrMet had low toxicity potential and relatively high safety margins in mice with the LD 50 value higher than 10.0 g/kg BW. These findings suggest that CrMet might be of potential value in the therapy and protection of diabetes.
研究领域:
药理学
·
毒理学
·
内分泌学
·
营养科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Rong Wu,Minmin Zou,Jiarui Cao,Manman Shi,Guobin Jiang,Fuliang Cao,Erzheng Su
Ginkgo seeds are rich in nutrients and bioactive compounds, but they cause poisoning after excessive consumption, thus limiting their application. In this work, enzymatic degradation technology was employed to remove ginkgotoxins presented in ginkgo seeds, including 4’-O-methylpyridoxine (MPN) and MPN-5’-glucoside (MPNG). Through the screening of four oxidases and ten reaction mediators, the laccase-syringaldehyde system was successfully established. After optimizing the reaction conditions using response surface methodology (RSM), ginkgotoxins in both raw and boiled ginkgo seed powder was completely degraded at a temperature of 40 °C and a solvent to solid ratio (60 mL/g) of the ginkgo seed powder suspension within 3 h. Furthermore, the main nutritional components of detoxified ginkgo seed powder did not exhibit significant changes, but there was inevitably a decrease in the content of bioactive compounds. Finally, an acute oral toxicity assessment demonstrated that the detoxified ginkgo seed powder was virtually non-toxic. Therefore, enzymatic degradation using the laccase-syringaldehyde system has the potential to be a simple and effective approach for removing ginkgotoxins from ginkgo seed powder, thereby meeting the safety requirements for ginkgo products.
研究领域:
食品科学
·
毒理学
·
酶学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）