Animals
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2022
·Vol. 12
·期 18
·2480
·
IF 3.231
Cheng Zhang,Weihao Li,Ligong Chen,Zhaoliang Chen,Xuejing Wang,Qianqian Xu,Hailong Zhang,Huan Chen,Juxiang Liu
Simple SummaryDeveloping safe and effective antibiotic growth promoters (AGPs) substitutes is particularly important to improve animal health and production performance. As an essential plant oil, the oregano oil's main bioactive substance is carvacrol, which has been proven to have antioxidant, anti-inflammatory, antibacterial, and antiviral properties. The sanguinarine from macleaya cordata is the primary bioactive substance. Sanguinarine has anti-tumor, immune-enhancing, antibacterial, and anti-inflammatory effects. However, it has not been reported whether the compatibility of oregano oil and macleaya cordata extract could produce better results. This study is the first to report the effect of oregano oil combined with macleaya cordata oral solution on the growth of broilers. The oregano oil combined with macleaya cordata oral solution significantly improved the growth performance of broilers. At the same time, serum biochemical indices, serum antioxidant indices, serum immune indices, serum cytokines, and intestinal morphology were significantly improved. In summary, our results demonstrated that the mixed solution of oregano oil and macleaya cordata has substantial potential to be an alternative to AGPs for broilers to reduce costs and improve benefits. This study provides basic data and technical support for further research.AbstractThe abuse of AGPs in animal husbandry has led to severe problems such as drug resistance and ecological, and environmental destruction, which seriously threaten human health and public health security. In recent years, extracts of oregano oil and macleaya cordata have become a hot spot in the research and application of AGP substitutes for their safety and high efficiency. This study is the first to report the effect of oregano oil combined with macleaya cordata oral solution on broiler growth performance. A total of 960 one-day-old broiler chickens were randomly divided into four treatment groups (240 chickens per group). Each treatment group was divided into six replicate groups (40 birds per replicate group). There were four groups in this study: the solvent control group, the oregano essential oil combined with macleaya cordata extract oral solution group (OS group), the oregano essential oil oral solution group (OEO group), and the macleaya cordata extract oral solution group (MCE group). Two chickens from each replicate group were collected and mixed into a composite sample. Six composite samples were obtained for each treatment group. The results showed that the oregano oil combined with macleaya cordata oral solution significantly improved the growth performance of broiler chickens. At the same time, serum biochemical indices, serum antioxidant indices, serum immune indices, serum cytokines, and intestinal morphology were significantly improved by the OS group. This study shows that oregano oil combined with macleaya cordata oral solution has substantial potential to be an alternative to AGPs for broilers.Keywords:oregano oil;macleaya cordata;oral solution;broilers;growth performance
研究领域:
动物科学
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药理学
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兽医学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Hui Wang,Mingyu Wang,Jinku Zhang,Di Wu,Zhezhe Qi,Xinbei Dong,Xing Zhang,Hongyuan Yan,Yehong Han
A urea-melamine-hexamethylenetetramine resin (UMHR) was synthesized using water as the reaction medium, with hexamethylenetetramine serving as an environmentally friendly crosslinking agent in place of traditional formaldehyde. The resin was optimized as an efficient solid-phase extraction (SPE) adsorbent through optimization of synthetic parameters. A precise detection method for berberine hydrochloride in Pudilan antiphlogistic oral liquid was established by optimizing extraction parameters. The method exhibited excellent linearity, with a correlation coefficient ( r ≥ 0.9998) over a concentration range of 0.40–100.0 μg/mL. The limits of detection (LOD) and quantitation (LOQ) were 0.094 μg/mL and 0.314 μg/ mL, respectively. Recoveries ranged from 87.9 % to 97.3 %, with relative standard deviations (RSDs) below 3.2 %. This study presents a novel and environmentally friendly analytical approach for the quality control of traditional Chinese medicine preparations.
研究领域:
分析化学
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药物分析
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环境化学
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DOI
PubMed
高影响因子
RSC Advances
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2022
·Vol. 12
·期 46
·30076-30084
·
IF 4.036
Ruiming Xiao,Hongzhang Chen,Hongbei Han,Guangjuan Luo,Ying Lin
Compound oral liquid (COL), made from functional herbal foods, has gained immense popularity in China for healthcare. However, the interaction between the nutrients in COL and gut microbiota is still unclear. In our study, the content of total flavonoids, polyphenols, and proteins was increased and the total sugar reduced by crushing raw ingredients to 10 mesh (COL-C). After 24 h incubation with supplemented COL by human gut microbiota, the results of 16S rRNA high-throughput sequencing revealed that Faecalibacterium, Collinsella, Bifidobacterium, Megamonas, Lactobacillus, Phascolarctobacterium, and Dialister were enriched by COL. In particular, the latter three genera were observed to be significantly enriched after incubation with COL-C. Meanwhile, the abundance of Dorea, Clostridium XIVa, and Escherichia/Shigella was inhibited by COL. Moreover, the increased levels of acetate, propionate, and butyrate in COL were jointly contributed by supplementary carbohydrates and the enrichment of short-chain fatty acid (SCFA)-producing bacteria. In summary, our results indicated that the optimized extraction facilitated the nutrients to be dissolved out and enhanced the potential prebiotic effects for promoting the abundance of probiotics, suggesting that the nutrients in COL-C might improve the microbial structure by strengthening the metabolism of beneficial bacteria and restricting the conditioned pathogens more efficiently.
研究领域:
微生物学
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营养学
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食品科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Ziru Dai,Baolan He,Saiyi Zhong,Mengyuan Xie,Jianfu Qiao,Qi Wang
With increasing environmental pressures and lifestyle changes, suboptimal skin health-such as impaired barrier function and photoaging-are becoming more prevalent. Functional foods have emerged as promising dietary interventions due to their efficacy, safety, and convenience. Oral liquids are favored for their ambient-temperature preparation, low energy demand, absence of organic solvents, and ability to preserve the bioactivity of natural compounds. In this study, we developed a composite oral solution based on oyster polysaccharides, tangerine peel, and sodium hyaluronate. Scanning electron microscopy (SEM) results demonstrated that the particles in the OP-TP-HA oral solution exhibited a uniform, nearly spherical morphology and good dispersion. ABTS radical scavenging rate of OP-TP-HA oral solution was 93.71 %. Further cell-based assays demonstrated enhanced cell proliferation and adhesion in the oral solution. Notably, it significantly promoted zebrafish tail fin regeneration, demonstrating its potential for skin health promotion. These findings provide a basis for the development of natural functional foods for skin repair.
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PubMed
高影响因子
Micromachines
·
2023
·Vol. 14
·期 7
·1368
·
IF 3.4
Yan Lang,Shuru Xu,Chunbin Zhang
In this paper, a molybdenum disulfide fluorescent probe with an Fe3+fluorescent system was first synthesized by the hydrothermal method for the detection of iron ion concentration in oral solution of protein succinate. It was characterized by infrared, fluorescence, X-ray photoelectron spectroscopy, scanning electron microscopy, and transmission electron microscopy. The probes were found to have good stability, photobleaching, and storage stability. The effects of dilution, pH, reaction time, and iron ion concentration on the fluorescent system were also investigated. The relative fluorescence intensity [(I0− I)/I0] showed a good linear relationship with the iron ion concentration in the range of 0–50 μM, with the linear equation [(I0− I)/I0] = 0.0148[Fe3+] + 0.0833 (r2= 0.9943, n = 11) and the detection limit of 2.43 μM. The reaction mechanism was also explored, as well as its ion selectivity, reversibility, accuracy, precision, and concentration of Fe ions in the actual sample. It was found that the probe can selectively detect Fe ions with a certain degree of reversibility, accuracy, precision, and ideal recovery, and it can be used for the determination of Fe3+in proteosuccinic acid oral solution.Keywords:MoS2quantum dots;fluorescent probe;Fe3+;performance;protein succinic acid oral solution
研究领域:
分析化学
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材料科学
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生物医学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Linke Xue,Linke Chen,Jing Dong,Lemei Cai,Yiru Wang,Xi Chen
Medicine safety has become a large concern and prompts an urgent need to develop a rapid, simple and sensitive analytical method, which can monitor excessive preservatives in medicine. In this work, dispersive liquid-liquid microextraction (DLLME) was combined with surface enhanced Raman scattering (SERS) for a quick analysis of a kind of preservatives, sodium benzoate , in ibuprofen oral solution. The experimental parameters affecting DLLME were systematically investigated. Under the optimal conditions, the whole procedure, including DLLME and the SERS analysis, could be carried out within 10 min. A good linearity between the concentration of sodium benzoate ranging from 10 to 500 mg L −1 and the SERS signal intensity could be obtained, and the correlation coefficient (R 2 ) 0.9986. The method detection limit was 0.56 mg L −1 . The relative standard deviation was less than 6.33% for ten replicates at the same sample concentrations. The analytical results prove that the method is suitable for rapid determination of sodium benzoate in ibuprofen oral samples.
研究领域:
分析化学
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药物分析
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DOI
PubMed
高影响因子
Current Analytical Chemistry
·
2017
·Vol. 13
·期 3
·250-255
·
IF 1.306
Luan Feng,Lu Yuxi,Tang Lili,Liu Huitao
Background: The safety and level of heavy metal ions in the traditional Chinese medicine has become an important topic. Most of the metal ions are carcinogens and lead to serious health concerns by producing free radicals. It is of great significance to develop simple and effective
methods to determine heavy metal ions. The aim of this paper is to develop a CE-indirect UV method for the analysis of the four heavy metal ions for Chinese medicine Shuanghuanglian oral liquid. Methods: The alternative CE method for separation and test of metal ions was developed in
this paper, which can help control the safety and level of heavy metal ions in traditional Chinese medicine Shuanghuanglian oral liquid. And the developed HPCE method showed superior analytical performance, including high sensitivity and good selectivity. Results: The experimental conditions
of the CE separation method were: 5s hydrodynamic injection at 0.5 psi, 20 kV applied voltage, and UV detection at 218 nm. The results show that the migration time of all the four heavy metal ions was less than 6 min. The calibration curves for four heavy metal ions were linear in the range
of 0.5-35 μg mL-1 with linear correlation coefficients of 0.9984-0.9999. The limits of detection were in the range of 0.08-0.61 μg mL-1 along with the recoveries of 93.0% to 109.5%. Conclusion: A simple, rapid and sensitive HPCE method has been developed to separate four heavy metal
ions including Cd2+, Cr3+, Cu2+ and Zn2+ in traditional Chinese medicine Shuanghuanglian oral liquid. The comprehensive analysis performance is satisfactory. The proposed HPCE method provides shorter analysis time and lower detection limit. The results demonstrated that the developed CE method
can be potentially applied as an good alternative for quality control of TCM.
研究领域:
中药分析
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药物安全
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 13
·4130
·
IF 4.927
Qiuhua Zhang,Lingyi Huang,Youjia Wu,Liying Huang,Xiaowen Xu,Renyi Lin
CompoundAnoectochilus roxburghii(Wall.) Lindl. (A. roxburghii) oral liquid (CAROL) is a hospital preparation ofA. roxburghiiandGanoderma lucidum(G. lucidum),which have hepatoprotective effects. Eight active components (five nucleosides/nucleobases and three triterpenoid acids) in CAROL,A. roxburghii, andG. lucidumwere simultaneously detected by high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS). The multiple reaction monitoring (MRM) mode was applied for the detection of analytes. These eight compounds were separated well within 12 min and quantified using the internal standard working curve method. The method showed good linearity (R2> 0.9935) and high sensitivity (limit of detection = 0.29 ng/mL). The analyte recovery ranged from 85.07% to 97.50% (relative standard deviation < 3.31%). The content of the target analytes in four batches of CAROL, and the raw materials ofG. lucidumandA. roxburghiifrom the five regions was determined using this method. The contents of guanosine and ganoderic acid A in four batches of oral liquid were high and stabilized and could be recommended as quality markers (Q-marker) for CAROL. Simultaneous qualitative and quantitative analysis of nucleosides and triterpenoid acids in CAROL,A. roxburghii, andG. lucidumby LC–MS/MS based on the MRM model was reported for the first time. The proposed method provides a sensitive, rapid, and reliable approach for the quality control of Chinese medicinal products.Keywords:LC–MS/MS;Anoectochilus roxburghii(Wall.) Lindl. and its compound oral liquid;Ganoderma lucidum;analysis;quality control
研究领域:
药用植物学
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分析化学
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药学
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中药学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2019
·
IF 0.931
Ting-Ting ZOU,Jin-Yi YANG,Zhen-Lin XU,Hong WANG,Yuan-Ming SUN,Hong-Tao LEI,Xue-Cai TAN,Yu-Dong SHEN
To monitor illegal addition of tadalafil and its analogues in health food, a new polyclonal antibody was prepared by immunizing New Zealand white rabbits with a common skeleton structure hapten combined with an unsaturated linear alkyl arm hapten strategy, which could simultaneously recognize tadalafil, amino tadalafil and N -Desmethyl tadalafil. A microwell lateral flow immune- chromatographic assay ( mw LFIA) method combined with simple sample pretreatment was developed. The mw LFIA showed that the LOD for three tadalafil drugs was between 0.05 ng mL −1 and 0.30 ng mL −1 , the cut-off values were between 50 ng mL −1 and 100 ng mL −1 . Cross-reactivity (CR) results showed no interference from substances with similar structures, indicating a good specificity against tadalafil, amino tadalafil and N -Desmethyl tadalafil. The average recoveries of three drugs in spiked health wine and oral liquid ranged from 78.7% to 117.8% with an RSD of less than 15%, showing a good aggreement with the results of HPLC-MS/MS method. The developed mw LFIA strip was suitable for the rapid detection of tadalafil and its analogues in health wine and oral liquid samples.
研究领域:
食品科学
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分析化学
·
免疫学
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Lele Gao,Liang Zhong,Tingting Feng,Jianan Yue,Qingqing Lu,Lian Li,Aoli Wu,Guimei Lin,Qiuxia He,Kechun Liu,Guiyun Cao,Zhaoqing Meng,Lei Nie,Hengchang Zang
The modernization and globalization of traditional Chinese medicine (TCM) face challenges such as unclear active compounds and inadequate quality control. Taking Xuefu Zhuyu Oral Liquid (XZOL) as an example, this study proposed an artificial intelligence (AI) -driven strategy for active compounds discovery and non-destructive quality control. Firstly, the multi-wavelength fusion high-performance liquid chromatography (HPLC) fingerprints were constructed to comprehensively characterize the chemical composition of XZOL. Secondly, the pro-angiogenesis effects of XZOL were evaluated in a PTK787-induced intersegmental vessels (ISVs) injury zebrafish model. Then, spectrum-effect relationship models, incorporating gray relational analysis (GRA), partial least squares regression (PLSR), backpropagation artificial neural networks (BP-ANN), and convolutional neural networks (CNN), discovered seven pro-angiogenesis active compounds (Hydroxysafflor Yellow A, Paeoniflorin, Ferulic Acid, Narirutin, Naringin, Hesperidin, and Neohesperidin). Furthermore, the efficacy of these compounds was further validated through network pharmacology, molecular docking, and zebrafish. Finally, a rapid and non-destructive quality control system based on near infrared spectroscopy (NIRS) was established. This system effectively distinguished expired and normal samples by combining Hotelling T 2 and Distance to Model X (DModX) statistics of multivariate statistical process control (MSPC), and accurately predicted the content of above active compounds by CNN model integration with bidirectional long short-term memory (Bi-LSTM) and multi-head self-attention (MHSA) networks. This study underscores the potential of AI-driven strategy to enhance TCM standardization and global recognition by providing an active compounds-based holistic quality control strategy of TCM.
研究领域:
中药学
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生物信息学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2017
·Vol. 41
·期 5
·e13180
·
IF 0.791
Yawei Zhang,Fengyuan He,Dengwu Li,Dongmei Wang
The effects of high temperature steam (HTS) treatment on liquid prepared from the leaves of Zanthoxylum bungeanum Maxim (ZBOL) were studied with by comparing other three treatment methods. After HTS treatment, the microbial content met the standard requirements, the ratios of the phytochemical content were all higher than 84.5%, the antioxidant activities had decreased by <9.57%, no obvious changes were observed in the sensory characteristics, and the results were all better than other treatments. Furthermore, ZBOL showed excellent chemical stability in assays in terms of the use of high temperature (60 ± 2°C) and strong illumination (4,500 ± 500 lx), and prediction and verification tests determined the shelf life of ZBOL to be 12 months (25°C). In summary, HTS was demonstrated to be an efficient treatment method of ZBOL, and these results can be used for the utilization of Z. bungeanum leaves in an industrial setting. Practical application This work provides an efficient method for treatment of oral liquid prepared with flavonoids and antioxidants from the leaves of Zanthoxylum bungeanum Maxim (ZBOL). Herein, the quality information of ZBOL is provided, and a theoretical basis is established for the market promotion of ZBOL and the comprehensive utilization of the resources of Z. bungeanum leaves.
研究领域:
食品科学
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药理学
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植物化学
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
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IF 4.8
Yanjun Yang,Zhouyang Qian,Chenhui Wu,Yue Cheng,Bing Yang,Jianguo Shao,Jing Zhao,Xiangjun Zhu,Xiaobin Jia,Liang Feng
Ethnopharmacological relevance Pudilan Xiaoyan Oral Liquid (PDL) is a proprietary Chinese medicinal preparation approved by the State for treating acute pharyngitis in both adults and children (Approval No. Z20030095). It is worth noting that children exhibit unique physiopathological characteristics compared to adults. However, the in vivo regulatory characteristics of PDL in treating acute pharyngitis in children remain incompletely understood. Aim of the study The differential absorption and metabolism characteristics of the main pharmacological components in PDL in young and adult rats were investigated with a view to providing a reference for preclinical data of PDL in medication for children. Materials and Methods This study utilized UPLC-Q-TOF-MS to investigate the pharmacodynamic material basis of PDL. The focus was on the gastrointestinal digestion and absorption characteristics of organic acid components in PDL (PDL-OAC), known as the primary pharmacodynamic components in this formulation. The research combined in vitro dynamic simulation and a Quadruple single-pass intestinal perfusion model to examine these characteristics. The permeability properties of PDL-OAC were evaluated using an artificial parallel membrane model. Additionally, an acute pharyngitis model was established to evaluate the histopathological condition of the pharynx in young rats using H&E staining. The levels of IL-1 β , TNF- α , IL-6, and IL-10 in blood and pharyngeal tissue homogenates of young rats were quantified using ELISA kits. Results A total of 91 components were identified in PDL, including 33 organic acids, 24 flavonoids, 14 alkaloids, 5 terpenoids and coumarins, 3 sugars, and 12 amino acids. The PDL-OAC exhibited a significant reduction in IL-1 β , TNF- α , IL-6, and IL-10 levels in the pharyngeal tissues of young rats with acute pharyngitis. Results from dynamic simulation studies of gastrointestinal fluids revealed that the PDL-OAC (Specifically chlorogenic acid (CGA), gallic acid (GA), chicoric acid (CRA), and caffeic acid (CA)) were effectively stabilized in the gastrointestinal fluids of both children and adults in vitro . Young rats, characterized by thinner intestinal walls and higher permeability, efficiently absorbed the four organic acids across the entire intestinal segment. The absorption of CGA, GA, and CRA followed a concentration-dependent pattern, with CGA and GA absorption being influenced by exocytosis. Conclusion The efficacy of the PDL-OAC in treating acute pharyngitis was demonstrated in young rats. The absorption rate of these components was observed to be faster in young rats compared to adult rats, underscoring the need for dedicated studies on the drug's usage in children. This research provides valuable insights for the appropriate clinical use of PDL in pediatric patients.
研究领域:
药理学
·
民族药理学
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儿科
·
药学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）