INTERNATIONAL MICROBIOLOGY
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2024
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IF 2.3
Wang Minyu,Chu Weihua
Oral opportunistic pathogen Fusobacterium nucleatum can participate in various disease processes through the metabolite hydrogen sulfide, such as halitosis and colorectal cancer. The object of this study is to identify inhibitor capable of suppressing Fn1220, which is the principal hydrogen sulfide-producing enzyme in F . nucleatum . Through this inhibition, we aim to reduce the hydrogen sulfide production of F . nucleatum , consequently diminishing its virulence. Employing molecular docking techniques for inhibitor screening, we identified dencichine as the monomeric compound from Chinese medicine exhibiting the lowest binding energy to Fn1220 among a set of 27,045 candidates, and evaluated in vitro the ability of dencichine to inhibit hydrogen sulfide production using bismuth chloride method. Additionally, we investigated its impact on key virulence factors, including biofilm formation, hemolysis, and adhesion factors of F . nucleatum , using the crystalline violet method, sheep blood method, and RT-qPCR, respectively. Furthermore, we assessed the influence of dencichine on the lifespan of Caenorhabditis elegans . Results showed that dencichine was a suitable inhibitor of the Fn1220 of F . nucleatum , which significantly inhibited the production of virulence factors, e.g., biofilm, hemolysin, FadA, and Fap2 of F . nucleatum and improved the survival of C . elegans . We successfully identified the inhibitor of the enzyme Fn1220, dencichine, which inhibited the production of hydrogen sulfide and attenuated the virulence of F . nucleatum and holds promise as a potential therapeutic avenue for addressing oral diseases, e.g., halitosis in the future.
研究领域:
微生物学
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药理学
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分子生物学
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传染病学
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口腔健康
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
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2025
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IF 5.1
Weijun Deng,Mengke Wang,Jing Hu,Huitao Wen
Discriminative and sensitive detection of low-concentration odorous gases is of great significance for health diagnostics, product quality control and robotics. However, achieving a biomimetic olfactory system in those applications with hydrogel remains a challenge. Here, by learning the mechanism of human olfactory epithelium, we design a flexible and ion-conductive hydrogel based on cellulose as an artificial nose. The hydrogel is soft, flexible, adhesive, self-healing and conductive, enabling the integration to various devices. The artificial nose exhibits high selectivity to H2S over other gas molecules such as NH3, NO2, CO2, CH3CH2OH, CH2O, etc. It has high linear sensitivity (0.34 ppm-1) with a fast response (143 s). More importantly, the sensoring range of this artificial nose moves into human olfactory threshold of H2S. The sensor is operated under low voltage (1 V) and mild temperature (0-25 °C) with sensoring stability for at least 2 weeks. Furthermore, this artificial nose are demonstrated as promising in applications of noninvasive halitosis diagnosis and meat spoilage identification. Our work may inspire odorant sensor development with artificial olfactory system for robotics and other specific applications.
研究领域:
传感器技术
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仿生工程
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材料科学
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分析化学
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健康诊断
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食品质量控制
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机器人技术
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
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2024
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IF 6.3
Xiaoyan Lang,Zhu Zhang,Xiaxia Xing,Xinhua Zhao,Zhenxu Li,Xiaoyu Chen,Yi Zhang,Dachi Yang
Hydrogen sulfide (H 2 S) sensing with ppb concentration detection limit and high selectivity is highly desired in future exhaled diagnoses of halitosis, asthma and down syndrome, however, needs further developing. Here, a ppb-concentration and highly selective H 2 S sensing has been developed with the two-dimensional cobalt and iron oxide nanosheets (CoFe x O y NSTs), which is transformed from leaf-shaped zeolitic imidazolate frameworks {ZIF-L(Co)} by combined Fe 2+ ion-etching and annealing. Typically, as-prepared CoFe x O y NSTs are of ∼ 10 nm thick with rough surface. Beneficially, the CoFe x O y NSTs present outstanding selectivity to H 2 S against other interfering gases and exhibit a detection limit as low as 50 ppb. Remarkably, the sensor prototypes exhibit a high response to 10 ppm H 2 S (R a /R g = 23.8) and shows fast response speed (∼13 s to 10 ppm H 2 S). Theoretically, such excellent H 2 S sensing might be attributed to the high surface ratio of CoFe x O y NSTs providing enriched adsorption sites for H 2 S molecules. Practically, an H 2 S sensing device built with the CoFe x O y NSTs sensor prototype is simulated for detecting H 2 S with reliable response, which is potential in future intelligent healthcare.
研究领域:
材料科学
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纳米技术
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传感器
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医疗技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）