FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Min Feng,Steven Suryoprabowo,Hong Tao,Liqiang Liu,Qiankun Zheng,Hua Kuang
Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO.
研究领域:
兽医学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2021
·
IF 3.232
Hongfei Liu,Xiaoya Xie,Chao Chen,Caleb Kesse Firempong,Yingshu Feng,Limin Zhao,Xuezhi Yin
Objective The purpose of the present study was to prepare a clonidine hydrochloride (CH) sustained-release suspension.Methods The processes involved in the drug formulation included drug loading, impregnating, and suspension preparation. Clonidine hydrochloride drug–resin complexes (CH-DRC) were prepared using the bath method and the CH-DRC impregnated before the microencapsulation process. Based on the bottom spray fluidized bed coating method, the CH microencapsulated drug–resin complexes (CH-MC) were also prepared using Surelease® (the suspension of ethyl cellulose aqueous dispersion) as the coating material. The effects of coating (process/formulation) on the in vitro release of coating microcapsule were evaluated via single factor investigation and orthogonal design optimization. The CH-MC with optimized formulation was further dispersed in a suitable medium to obtain a sustained-release suspension. Rats were given commercial CH ordinary tablets and the CH sustained-release suspension via intragastric administration. The plasma concentration–time curve and related pharmacokinetic parameters were investigated using the non-compartment model.Results The Tmax of the CH sustained-release suspension was delayed from 2 h to 5 h compared with the CH ordinary tablets. Similarly, the Cmax was reduced from 32.138 µg·mL−1 to 18.150 µg·mL−1 with the concentration–time curve being more gentle compared with the commercially CH ordinary tablets. After oral administration, the relative bioavailability of CH sustained-release suspension (AUC0–24 of 137.703 µg·h·mL−1) to its CH ordinary tablets (AUC0–24 of 123.337 µg·h·mL−1) was 111.65%.Conclusions The findings showed that the CH sustained-release suspension for oral administration was successfully formulated.
研究领域:
药剂学
·
药物递送系统
·
生物药剂学
✓ 已验证
DOI
高影响因子
MACROMOLECULES
·
2024
·
IF 5.1
Chi Zhang,Jiang-An You,Xue Wang,Rui Hou,Xiaofeng Li,Yuxuan Sun,Guorui Qin,Shenghai Li,Suobo Zhang
By examining the structural patterns of the imine monomers engaged in polycondensation, a series of poly(ether ether ketone) precursor polymers (PEEKi-N-acyl) modified with N-acyl Imine groups were designed in this work. Contrary to poly(ether ether ketone) (PEEK), these precursor polymers have superior solubility, enabling their production in solution and processing of membrane components. The membrane components’ substance could undergo an imine hydrolysis reaction in an acidic environment, turning them back into PEEK and giving them solvent resistance. In addition, the homogeneous polymer membranes of this series exhibit spontaneous pore formation under the action of ethanol, which was caused by solvent-induced internal stress relaxation. By utilizing the properties of this type of polymer, precursor polymers can be processed into PEEK sponge-like porous membranes, expanding the processing morphology and application range of PEEK.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Linlin Chen,Xiaofei Hu,Yaning Sun,Yunrui Xing,Gaiping Zhang
In this study, a novel hapten was designed and successfully synthesized to prepared the artificial immunogen against xylazine (XYL), then the monoclonal antibody 1H2-2C6 was obtained and applied to a lateral flow immunoassay (LFI) strip for rapid detection of XYL in milk. The visual detection limit (cutoff value) of the LFI strip was 5 ng/mL, the linear regression formula was Y = −0.521lg(X) + 0.382 ( R 2 = 0.995, n = 6), the linear detection range was 0.16–2.23 ng/mL and the detection limit (LOD) was 0.1 ng/mL. The recovery rates were in the range from 88.95% to 91.45%, the highest coefficient of variation was 8.89% (n = 3). The LFI strip was also validated by LC-MS/MS, as revealed, there were no significance differences between the two methods (the confidence interval was 95%). In brief, the LFI strip provides a reliable, sensitive and portable testing tool for screening of XYL on-site.
研究领域:
分析化学
·
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）