Heng Zhang,Xuran Xu,Chuntao Chen,Xiao Chen,Yang Huang,Dongping Sun
In an attempt to obtain bacterial cellulose (BC) with controllable pores, Ca-alginate particles were introduced into BC fermentation medium. Ca-alginate particles with different diameters were prepared through electrostatic spinning and pulverization. After removing the particles, a continuous and homogeneous network of pores with diameters around 5 μm, 20 μm, and 400 μm were presented inside BC hydrogel. Compared to native BC, the crystallinity, the surface area and water holding capacity (WHC) were all increased when the pore sizes around 5 μm, 20 μm. However, the porous BC with diameters around 400 μm showed poor performance in WHC and mechanical strength. This novel approach described in this paper introduced a new approach for creating controllable porous BC with user-defined control over the physical dimensions of the network, and slightly changed its structure.
Due to the fast degradation time and low storage temperature of nucleic acid, a simple, fast, and highly efficient adsorption process is imperious for the extraction of nucleic acid molecules. In this article, a poly(vinyl alcohol- co -ethylene) (EVOH) nanofiber-based and polyethyleneimine (PEI)-modified chitosan (ENPC) aerogel with high static adsorption capacity of RNA and DNA molecules (812 and 867 mg g −1 , respectively) is proved for enormous potential in adsorption capacity for nucleic acid extraction. A relation between adsorption capacity and the physical morphology of ENPC aerogel membrane is demonstrated with the controllable factors such as chemical components, freeze-drying temperature, pH value of the nucleic acid solution, and membrane density. The mechanism of the anion-exchange adsorption process of nucleic acid is analyzed and fitted well with the pseudo-second-order kinetic model and Langmuir isotherm (monolayer adsorption). The as-obtained ENPC aerogel in the form of a membrane with self-cleaning and flexible properties can be easily tailored and widely utilized in a convenient and efficient extraction process of nucleic acids in both industrial and experimental levels.
危险品化学品经营许可证（带存储）