JOURNAL OF BIOTECHNOLOGY
·
2024
·
IF 4.1
Bingbing Xia,Zhiwei Li,Jinao Wei,Ganpeng Hu,Jingwen Yang,Hongbin Zhang,Xueqin Hu
Dextranases are hydrolases that exclusively catalyze the disruption of α−1,6 glycosidic bonds . A series of variant enzymes were obtained by comparing the sequences of dextranases from different sources and introducing sequence substitutions. A correlation was found between the number of amino acids in the 397–401 region and the hydrolytic process. When there were no more than 5 amino acids in the 397–401 region, the enzyme first hydrolyzed the dextran T70 to a low molecular weight dextran with a molecular weight of about 5000, then IMOs 1 appeared in the system if the degradation continued, showing a clear sequential relationship. And when there are more than 5 amino acids in the 397–401 region, IMOs were produced at the beginning of hydrolysis and continue to increase throughout the hydrolytic process. At the same time, we investigated the enzymatic properties of the variants and found that the hydrolytic rate of A-Ca was 11 times higher than that of the original enzyme. The proportion of IMOs produced by A-Ca was 80.68%, which was nearly10% higher than the original enzyme, providing a new enzyme for the industrial preparation of IMOs.
研究领域:
生物化学
·
酶学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Hongying Su,Qingming Jia,Shaoyun Shan
Polysaccharide-based microgels with high water content, excellent biocompatibility and controllable particle size have been widely studied as ideal candidates for drug release and delivery. In this study, microgels based on dextran were developed via the Schiff base formation between aldehyded dextran and ethylenediamine in a water-in-oil (W/O) microemulsion. Particle size of the resulted microgel was controllable between 800 and 1100 nm by modulating the amount of the employed co-surfactants (Span 80/Tween 80). Furthermore, fluoresceins ( e.g. , aminofluorescein) and drugs ( e.g. , doxorubicin) with free amino groups can be conjugated onto the network of the dextran-based microgel via Schiff base linkages. Since the Schiff base linkages are degradable via hydrolysis and their stability decreases with the environmental pH decreases, the resulted Schiff bases contained microgel showed a pH dependent degradation profile. These results indicated that the pH-sensitive microgel based on dextran could be used as promising drug delivery systems for biomedical applications.
研究领域:
生物材料
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Yan Chen,Li Liao,Jin Liu,Hongxia Gao,Xing Chen,Lexiang Zhang,Liqiang Zou
Vanillin is widely used as a food flavoring agent for its pleasant aroma and biological properties, but its volatility during high-temperature processing limits its effectiveness. In this study, ternary complex microparticles consisting of vanillin/γ-cyclodextrin/dextran (V/C/D) were prepared and applied in bread manufacturing. The retention rate of vanillin during bread baking increased from 44.25% to 69.92% as the proportion of dextran in the complex microparticles (V/C/D 1:9:0-V/C/D 1:5:4) was enlarged. Furthermore, enhanced vanillin retention within the bread improved its resistance to oxidation and spoilage during storage. Incorporating vanillin-complexed microparticles into bread delayed increases in acid value, peroxide value, and total colony count during storage at 37 °C, extending the bread's shelf life from 4 to 6 days. Vanillin retained in the bread inhibited microbial growth, significantly reducing the relative abundance of Bacillus (from 81.98% in blank bread to 7.54% in V/C/D 1:5:4) and Aspergillus flavus (from 99.94% in blank bread to 83.00% in V/C/D 1:5:4) during storage. The results from this research could offer valuable insights into enhancing the quality of natural flavorings and baked goods.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Wen Li,Liyuan Yun,Yunjiao Zhao,Zijian Zhi,Esther Mwizerwa Muhindo,Xiaoyuan Geng,Rui Liu,Tao Wu,Wenjie Sui,Min Zhang
Dextran is widely used as a model polysaccharide to study the interactions between polysaccharides and small molecule sugars. This study examined water sorption isotherms and glass transition temperatures ( T g ) of mixtures of dextran with trehalose , lactose and sucrose for understanding how different disaccharides affect dextran's processing adaptation and storage performance relevant to water sorption. At the same chemical composition , monolayer water ( m 0 ) was dextran & sucrose (dex&suc) > dextran & trehalose (dex&tre) > dextran & lactose (dex&lac). The higher ratios of dextran, the higher was the Guggenheim constant ( C ) value of the mixtures. The compatibility of dextran and disaccharides was dex&tre > dex&lac > dex&suc. Gordon–Taylor model showed dex&tre (2:1) best inhibited water plasticization . Furthermore, trehalose and lactose were more effective in preventing morphological changes of hydrous matrices in the mixtures. The crystallinities of samples at 43% relative humidity (RH) were slightly lower than that at 11% RH.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2011
·
IF 3.463
Haibo Li,Rui Niu,Jinliang Yang,Jun Nie,Dongzhi Yang
A biodegradable tissue adhesive was prepared from dextran urethane (Dex-U), which could be photocrosslinked under the UV irradiation, and steadily adhere to surface of gelatin and mouse skin, which simulate the human tissue. The structures of above Dex-U were characterized by FTIR, 1 H NMR spectroscopy and XRD. The adhesion properties of result products was evaluated by lap-shear test and burst pressure test. The highest adhesion strength and burst pressure could reach to 2.99 MPa and 35 mmHg, respectively. The photopolymerization process of Dex-U was monitored by real time infrared spectroscopy (RTIR), and the surface tension of Dex-U solution was tested by du Nouy Ring method. The cytotoxicity of Dex-U also was evaluated. The relationship between all above properties and degree of substitution of Dex-U was assessed. The adhesive strength of Dex-U is significantly higher than that of fibrin glue. The obtained products present the potential to serve as tissue adhesive in medical area in the future.
研究领域:
生物材料学
·
医学工程
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Yanfang Hu,Liang He,Jianxun Ding,Diankui Sun,Li Chen,Xuesi Chen
Graphene-based nanocarriers show great potential in photo-chemotherapy, however, to prepare desired reduced graphene oxide (rGO) nanoparticles in a facile way is still a challenge. Herein, a novel strategy has been presented to prepare rGO nanoparticle using dextran (Dex) as a reducing agent. In this strategy, Dex was directly conjugated on rGO by hydrogen bond and then self-assemble to form rGO/Dex nanoparticles. After decorated by dextran, rGO-based nanoparticles not only show excellent biocompatibility but also can load anticancer drug for photo-chemotherapy. The data of fourier transform infrared (FT-IR) analysis, Raman spectrum analysis, thermos-gravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), the transmission electron microscope (TEM) image and dynamic light scattering (DLS) measurements powerfully proved that the stable rGO-based nanoparticles with desired nanosize have been successfully prepared. To verify the photo-chemotherapy, anticancer drug, doxorubicin (DOX), has been loaded on rGO/Dex nanoparticles (rGO/DOX/Dex). And RGD, a kind of oligopeptide which can improve the intracellular uptake by α v β 3 recognition, also has been introduced (rGO/DOX/RDex). Compared with single chemotherapy, rGO/DOX/Dex and rGO/DOX/RDex combining the local specific chemotherapy and external near-infrared (NIR) photo-thermal therapy show higher therapeutic efficacy, endowing the desired rGO-based nanoparticle with great potential for cancer treatments.
研究领域:
纳米医学
·
肿瘤学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 4
·2931-2937
·
IF 1.354
Zhu Changhao,Ma Xiaoe,Ma Dandan,Zhang Tianzhu,Gu Ning
For computed tomography (CT) angiography and drug release application, a kind of novel dextran hydrogel microspheres were prepared. β-cyclodextrin (β-CD) grafted poly(methyl vinyl ether-altmaleic acid) (PMVE-alt-MA-g-β-CD) and succinic acid modified
dextran (Dex-SA) were first prepared, and then PMVE-alt-MA-g-β-CD was further used as the cross-linking agent to cross link Dex-SA for the formation of dextran hydrogel microspheres by using an inverse suspension polymerization method for the potential interventional embolization.
The average diameter of the dextran hydrogel microspheres was 35 μm with 90% ranging from 20 μm to 50 μm. The obtained microspheres showed a rather high swelling rate and loading capacity of drug doxorubicin hydrochloride with content of 9.2 wt%. The results of
in vitro experiments showed that about 35.5% of the total amount of the encapsulated doxorubicin hydrochloride can be released after 4 h at 37 °C. The microspheres had a good mechanical stiffness with Young’s modulus of about 20.0 kPa. Iodine molecules (I2) can
be incorporated within the cavity of grafted cyclodextrin only through simply soaking in I2 aqueous solution, and these I2-loaded microspheres can preliminarily realize the function of CT angiography. This kind of dextran hydrogel microspheres with good biocompatibility would be
a promising embolization material.
研究领域:
生物材料学
·
医学影像学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Zhi-Wei Guo,Heng-Juan Li,Ning Peng,Ying-Qiu Li,Yan Liang,Yu-Ru Zhao,Cai-Yue Wang,Zi-Yue Wang,Chenying Wang,Xidong Ren
BACKGROUND Pea protein isolate (PPI) is gaining increasing popularity in the food industry. It provides a diverse range of health benefits, such as hypoallergenic and gluten-free characteristics. However, the functional performance of PPI is hindered by its low solubility and poor stability. Therefore, in this article, PPI and dextran (DX) of different molecular weights were grafted to investigate the effects of grafting DX with different molecular weights on the interface properties and antioxidant properties of PPI. Additionally, the stability and digestive properties of the glycated PPI nanoemulsion system were explored. RESULTS The result showed that the grafting degree of PPI–DX conjugates (PPI–DC) decreased with an increase in the molecular weight of DX. Surface hydrophobicity, antioxidant activity and solubility of PPI–DC were significantly improved after grafting compared with PPI and PPI–DX mixtures (PPI–DM). Astaxanthin-loaded emulsions stabilized by grafted conjugates had smaller droplets and higher astaxanthin encapsulation rate compared to PPI emulsions. In vitro digestion demonstrated that the bioavailability of PPI–DC emulsions was higher than of PPI emulsion. Furthermore, after 24 days of storage, retention rate of astaxanthin-loaded emulsions prepared by conjugates remained above 70%, surpassing that of PPI emulsion. CONCLUSION These results indicated that DX grafting can improve the emulsion properties of PPI. In addition, the DX with a molecular weight of 5 kDa showed the most significant improvement. This study contributes to the advancement of natural emulsifiers by modifying PPI through glycation, and furnishes a valuable reference for its utilization in functional foods. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Polymers
·
2021
·Vol. 13
·期 9
·1515
·
IF 4.329
Xiukun Xue,Yanjuan Wu,Xiao Xu,Ben Xu,Zhaowei Chen,Tianduo Li
Polymeric prodrugs, synthesized by conjugating chemotherapeutic agents to functional polymers, have been extensively investigated and employed for safer and more efficacious cancer therapy. By rational design, a pH and reduction dual-sensitive dextran-di-drugs conjugate (oDex-g-Pt+DOX) was synthesized by the covalent conjugation of Pt (IV) prodrug and doxorubicin (DOX) to an oxidized dextran (oDex). Pt (IV) prodrug and DOX were linked by the versatile efficient esterification reactions and Schiff base reaction, respectively. oDex-g-Pt+DOX could self-assemble into nanoparticles with an average diameter at around 180 nm. The acidic and reductive (GSH) environment induced degradation and drug release behavior of the resulting nanoparticles (oDex-g-Pt+DOX NPs) were systematically investigated by optical experiment, DLS analysis, TEM measurement, and in vitro drugs release experiment. Effective cellular uptake of the oDex-g-Pt+DOX NPs was identified by the human cervical carcinoma HeLa cells via confocal laser scanning microscopy. Furthermore, oDex-g-Pt+DOX NPs displayed a comparable antiproliferative activity than the simple combination of free cisplatin and DOX (Cis+DOX) as the extension of time. More importantly, oDex-g-Pt+DOX NPs exhibited remarkable reversal ability of tumor resistance compared to the cisplatin in cisplatin-resistant lung carcinoma A549 cells. Take advantage of the acidic and reductive microenvironment of tumors, this smart polymer-dual-drugs conjugate could serve as a promising and effective nanomedicine for combination chemotherapy.Keywords:polymeric prodrug;dual-sensitive;combination chemotherapy;drug conjugation;dextran
研究领域:
肿瘤学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 101
·期 15
·6355-6367
·
IF 3.639
Shiyuan Luo,Abdullah Saadi,Kai Fu,Maierhaba Taxipalati,Lingli Deng
BACKGROUND In recent years, there has been considerable interest in the use of biopolymer electrospun nanofibers for various food applications due to the biocompatibility, biodegradability, and high loading capacity. Herein, we fabricated and characterized novel hybrid electrospun fibers from dextran (50%, w/v ) and zein (0–30%, w/v ) solutions, and the effects of various zein concentrations on the properties of the hybrid electrospun fibers were investigated. RESULTS When zein was added at low concentrations (5% and 10%), dextran and zein showed poor miscibility, as reflected by significantly decreased viscosity of the solutions, and the poor mechanical properties of the derived fiber membranes. When zein was added at medium concentrations (15–25%), hydrogen bonds were formed between dextran and zein molecules, as indicated by the red shift of Fourier-transform infrared bands and β-sheet to α-helix structural transformations. The fiber membranes electrospun from a solution with 25% zein showed the most hydrophobic surface, with a water contact angle of 116.9°. The homogenous dispersion of dextran and zein resulted in improved mechanical properties for fibers electrospun from a solution with 30% zein. Curcumin encapsulating dextran/zein electrospun fibers exhibited effective radical scavenging activity and ferric reducing power, along with the desired controlled release behavior for curcumin delivery. CONCLUSION Food grade dextran/zein hybrid electrospun fibers demonstrated tunable properties, and appear to be promising as delivery systems for bioactive and edible antimicrobial food packaging. © 2021 Society of Chemical Industry.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 135
·期 6
·45831
·
IF 1.86
Jing Zhang,Liqun Chen,Jiada Chen,Dan Wu,Jie Feng
We demonstrated an extremely facile way to fabricate inorganic–organic microgels with pH sensitivity and fluorescence. Aqueous dextran microgels are crosslinked by ZnO quantum dots (QDs). The ZnO@Dextran microgels were synthesized by simply mixing amino-modified ZnO (ZnO QDs) with carboxymethyl dextran (CMD) while stirring. The hybrid microgels showed an average diameter of ∼5 μm and strong fluorescence under ultraviolet (365 nm) irradiation. Up to 79.3 wt % of ZnO QDs were loaded into microgels. The ZnO QDs crosslinkage in the hybrid microgels structure enabled the microgels to degrade under mild acidic environment due to pH sensitivity of ZnO QDs. After loading of doxorubicin (Dox), the microgels were used as drug carriers for pH-controlled release of Dox. The degradation of the microgels and the release of loaded cargos could be monitored by detecting fluorescence intensity of the microgels. Moreover, owing to the cytotoxicity of ZnO QDs at their destination, drug-loaded ZnO@Dextran microgels can be used for synergistic therapy. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 45831.
研究领域:
材料科学
·
生物医学工程
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Yu Zhao,Xinge Zhang,Yanan Wang,Zhongming Wu,Jinxia An,Zhentan Lu,Lin Mei,Chaoxing Li
Many synthetic hydrogels for drug delivery have been based on polyethylene glycol which is non-natural, non-biodegradable and only terminal-functionalizable. The polysaccharides dextran and chitosan not only are highly hydrophilic, biodegradable and pendant-functionalizable, but also more closely mimic the nature extracellular matrix glycosaminoglycans. Here, a biomimetic hydrogel based on chitosan and dextran was synthesized by the Michael addition reaction. The hydrogels have good swelling and cytocompatibility against NIH3T3. Moreover, vancomycin-loaded hydrogels were formed in situ, and could kill both Gram-positive bacteria and Gram-negative bacteria, indicating that the hydrogel as a wound dressing could provide protection against bacterial infection. Notably, the drug release was controlled via modifying the compositions. Therefore, the biomimetic polysaccharide hydrogels as a promising carrier have potential application for wound healing.
研究领域:
生物材料学
·
药物递送系统
·
感染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）