Hemerocallis citrina Baroni ( H. citrina Baroni) is widely used in the food and medical industries. However, a comprehensive evaluation of the physicochemical, rheological properties, and hypoglycemic activities of its various parts has not yet been reported. In this study, we extracted polysaccharides from the leaves (HCLP) and stems (HCSP) of H. citrina Baroni. The physicochemical properties were investigated using high-performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), Congo red experiment, Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), thermogravimetric (TG), and scanning electron microscopy (SEM). HCSP exhibited higher total sugar contents, uronic acids, protein, total phenol, and flavonoid contents than HCLP. However, the HCSP extraction yield (2.54 ± 0.68%) was lower than that of HCLP (6.34 ± 0.75%). Both polysaccharides were mainly composed of Mannose (Man) and galactose (Gal), but their contents varied significantly; HCSP contained more glucosamine (GlcN), rhamnose (Rha), arabinose (Ara), gluconic acid (GlcA), galacturonic acid (GalA) and glucose (Glc) than HCLP. The results showed that both HCSP and HCLP displayed macromolecular characteristics with a triple-helix structure. The thermal stability was stronger in HCLP than in HCSP. The rheological results showed that HCLP and HCSP exhibited a pseudoplastic fluid behavior. Compared to HCSP, HCLP had a stronger ability to inhibit α -glucosidase and enhance glucose consumption in HepG2 cells, attributed to its superior thermal stability and higher molecular weight ( M w ). These results indicated that polysaccharides from the leaves and stems of H. citrina Baroni have potential as functional foods and hypoglycemic drugs.
Although Abelia macrotera has long been used as the raw material for the special snack “immortal tofu” by Chinese population, the composition and activity of its leaf and stem are rarely studied. Ultrahigh-performance liquid chromatography-quadrupole (UHPLC-Q)-Exactive Orbitrap mass spectrometry and various experiments were used for in vitro study of its chemical composition and antioxidant, antityrosinase, and anti-inflammatory activities . A fast, accurate, and sensitive UHPLC-Q-Exactive Orbitrap mass spectrometry method has been established and effectively used for the determination of the chemical composition of the leaf and stem of A. macrotera . The biological activities of the leaf and stem extracts of the A. macrotera were determined in vitro by antioxidant and anti-inflammatory methods. A total of 142 chemical constituents including 48 flavonoids, 65 organic acids, 12 phenylpropanoids, and 19 others were identified by UHPLC-Q-Exactive Orbitrap mass spectrometry. The results show that the leaf and stem extracts have wide and significant antioxidant capacities, including scavenging effects of DPPH, ABTS and hydroxyl radicals, and the ability to reduce both iron and copper metal ions. Additionally, they showed a strong inhibition of NO production, with a dose-dependent inhibition. To the best of our knowledge, this is the first study to report the identification and content determination of the chemical components of the A. macrotera leaf and stem. These results will be beneficial to further understand its medicinal value.
危险品化学品经营许可证（带存储）